Restriction map for
pFN21AE2635
(Circular) (Six-base) MAPSORT of: pFN21AE2635.seq Check: 3273 from: 1 to: 5875
pFN21AE2635 5875 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2605 3938 5322 5590 1632
Size: 624 349 1333 1384 268 1917
Fragments arranged by size:
1917 1384 1333 624 349 268
AflII C'TTAA_G
Cuts at: 820 1017 2696 820
Size: 197 1679 3999
Fragments arranged by size:
3999 1679 197
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 2163
Size: 32 5843
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2064 2198 2241 2318 2999 3184 3410 3562
Size: 571 134 43 77 681 185 226 152
Cuts at: 3562 5671 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 681 571 226 185 152 134
77 43
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2825 3200 1870
Size: 73 882 375 4545
Fragments arranged by size:
4545 882 375 73
BamHI G'GATC_C
Cuts at: 3097 3205 3572 3097
Size: 108 367 5400
Fragments arranged by size:
5400 367 108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2605 3196 4651 618
Size: 325 217 627 818 591 1455 1842
Fragments arranged by size:
1842 1455 818 627 591 325 217
BanII G_rGCy'C
Cuts at: 1391 3194 1391
Size: 1803 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 928
Size: 971 926 3978
Fragments arranged by size:
3978 971 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 3936 5092 1790
Size: 2146 1156 2573
Fragments arranged by size:
2573 2146 1156
BmgBI CAC'GTC
Cuts at: 1318 2452 2899 1318
Size: 1134 447 4294
Fragments arranged by size:
4294 1134 447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 4535 1239
Size: 951 2345 2579
Fragments arranged by size:
2579 2345 951
Bpu10I CC'TnA_GC
Cuts at: 2520 2810 2520
Size: 290 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2727 3123 4000 4865 5163 2727
Size: 396 877 865 298 3439
Fragments arranged by size:
3439 877 865 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 3123 5869 3123
Size: 2746 3129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2163 2193 1178
Size: 30 165 30 760 30 4860
Fragments arranged by size:
4860 760 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5875
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3936 4021 5092 3936
Size: 85 1071 4719
Fragments arranged by size:
4719 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3194 3936 4021 5092 1391
Size: 399 1404 742 85 1071 2174
Fragments arranged by size:
2174 1404 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2529 3764 5494 2529
Size: 1235 1730 2910
Fragments arranged by size:
2910 1730 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2425 3230 844
Size: 1581 805 3489
Fragments arranged by size:
3489 1581 805
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 3762 1394
Size: 429 1007 932 3507
Fragments arranged by size:
3507 1007 932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3088 4370 4544 65
Size: 1183 1840 1282 174 1396
Fragments arranged by size:
1840 1396 1282 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2669 794
Size: 1875 4000
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2150 1184
Size: 966 4909
BstYI r'GATC_y
Cuts at: 1972 3097 3205 3572 3957 3974 5426 5512 5524
Size: 1125 108 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1125 385 367 346 108 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3439
Fragments arranged by size:
3439 1884 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2667 3391 4185 4205 2667
Size: 724 794 20 4337
Fragments arranged by size:
4337 794 724 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 2323 3179 2323
Size: 856 5019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2952 3322 4211
Size: 54 1425 50 275 18 1122 370 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1122 889 370 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 3805 1353
Size: 1049 1403 3423
Fragments arranged by size:
3423 1403 1049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2006 2675 4462 4836 4982 387
Size: 1064 138 417 669 1787 374 146 1280
Fragments arranged by size:
1787 1280 1064 669 417 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2256 2605 3938 4535 5322 5590
Size: 393 558 66 349 1333 597 787 268
Cuts at: 5590 1239
Size: 1524
Fragments arranged by size:
1524 1333 787 597 558 393 349 268
66
EcoO109I rG'GnC_Cy
Cuts at: 2444 2524 2444
Size: 80 5795
EcoRI G'AATT_C
Cuts at: 2064 3184 3562 2064
Size: 1120 378 4377
Fragments arranged by size:
4377 1120 378
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2495 5022 1678
Size: 817 2527 2531
Fragments arranged by size:
2531 2527 817
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373
Size: 32 135 32 2944 32 42 32 2626
Fragments arranged by size:
2944 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 10
Size: 54 1425 343 1122 2931
Fragments arranged by size:
2931 1425 1122 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3031 3833 4192 4351 518
Size: 648 1320 545 802 359 159 2042
Fragments arranged by size:
2042 1320 802 648 545 359 159
MspA1I CmG'CkG
Cuts at: 1606 3291 3968 5116 5361 1606
Size: 1685 677 1148 245 2120
Fragments arranged by size:
2120 1685 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3439
Fragments arranged by size:
3439 1884 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2142 2142
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2163 2195 3944 3976 5457 5489 2163
Size: 32 1749 32 1481 32 2549
Fragments arranged by size:
2549 1749 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2890 1613
Size: 1277 4598
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspXI vC'TCGA_Gb
Cuts at: 2825 2825
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2356 3227 830
Size: 1526 871 3478
Fragments arranged by size:
3478 1526 871
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2352 2595 3223 4358 5039 5230 826
Size: 219 1307 243 628 1135 681 191 1471
Fragments arranged by size:
1471 1307 1135 681 628 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 2742 2825 3138 3979 4880
Size: 197 926 753 46 83 313 841 901
Cuts at: 4880 5142 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 841 753 313 262 197
83 46
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2940 2949 3314 513
Size: 552 1875 9 365 3074
Fragments arranged by size:
3074 1875 552 365 9
SwaI ATTT'AAAT
Cuts at: 2323 2323
Size: 5875
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2169 3139 3902 3919 4072 4257 4702
Size: 1255 589 970 763 17 153 185 445
Cuts at: 4702 325
Size: 1498
Fragments arranged by size:
1498 1255 970 763 589 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2261 2458 4121
Size: 274 80 33 51 1443 284 197 1663
Cuts at: 4121 5833 96
Size: 1712 138
Fragments arranged by size:
1712 1663 1443 284 274 197 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2321 2353 5835 5867 2121
Size: 32 168 32 3482 32 2129
Fragments arranged by size:
3482 2129 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2947 2989 2947
Size: 42 5833
XhoI C'TCGA_G
Cuts at: 1943 2825 1943
Size: 882 4993
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 2615 4004 2615
Size: 1389 4486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI