Restriction map for
pFN21AE2624
(Circular) (Six-base) MAPSORT of: pFN21AE2624.seq Check: 5934 from: 1 to: 5485
pFN21AE2624 5485 bp
With 182 enzymes: SgfI *
May 26, 2016 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2586 2586
Size: 5485
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4932 5200 1632
Size: 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2341 2614 2695 3228 4800 2341
Size: 273 81 533 1572 3026
Fragments arranged by size:
3026 1572 533 273 81
ApaI G_GGCC'C
Cuts at: 2110 2170 2607 2110
Size: 60 437 4988
Fragments arranged by size:
4988 437 60
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2567 2794 3020 3172 5281 1493
Size: 1074 227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 227 226 152
AscI GG'CGCG_CC
Cuts at: 2179 2179
Size: 5485
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2779 2810 1870
Size: 73 836 31 4545
Fragments arranged by size:
4545 836 73 31
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2233 2246 2296 2410
Size: 325 217 627 253 193 13 50 114
Cuts at: 2410 2806 4261 618
Size: 396 1455 1842
Fragments arranged by size:
1842 1455 627 396 325 253 217 193
114 50 13
BanII G_rGCy'C
Cuts at: 1391 2110 2170 2391 2607 2804 1391
Size: 719 60 221 216 197 4072
Fragments arranged by size:
4072 719 221 216 197 60
BbeI G_GCGC'C
Cuts at: 2044 2237 2300 2414 2044
Size: 193 63 114 5115
Fragments arranged by size:
5115 193 114 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4096 136
Size: 107 122 71 631 605 343 2081 1525
Fragments arranged by size:
2081 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2170 2607 3546 4702 1790
Size: 320 60 437 939 1156 2573
Fragments arranged by size:
2573 1156 939 437 320 60
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 4303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3216 4174 442
Size: 416 53 83 399 24 1799 958 1753
Fragments arranged by size:
1799 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 4145 1239
Size: 1049 1857 2579
Fragments arranged by size:
2579 1857 1049
Bpu10I CC'TnA_GC
Cuts at: 2744 2744
Size: 5485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3610 4475 4773 3610
Size: 865 298 4322
Fragments arranged by size:
4322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 4148 882
Size: 617 1119 1530 2219
Fragments arranged by size:
2219 1530 1119 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 2234 2273 2297
Size: 53 83 186 936 508 193 39 24
Cuts at: 2297 2411 2421 3292 3674 4309 275
Size: 114 10 871 382 635 1451
Fragments arranged by size:
1451 936 871 635 508 382 193 186
114 83 53 39 24 10
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5260
Fragments arranged by size:
5260 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2270 2717 1186
Size: 852 232 447 3954
Fragments arranged by size:
3954 852 447 232
BseYI C'CCAG_C
Cuts at: 2095 2203 2237 2527 2607 4688 5184 2095
Size: 108 34 290 80 2081 496 2396
Fragments arranged by size:
2396 2081 496 290 108 80 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2558 2786 1163
Size: 573 618 204 228 3862
Fragments arranged by size:
3862 618 573 228 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2174 2488 2935 3696 4724 1540
Size: 275 186 173 314 447 761 1028 2301
Fragments arranged by size:
2301 1028 761 447 314 275 186 173
BsiHKAI G_wGCw'C
Cuts at: 2670 3546 3631 4702 2670
Size: 876 85 1071 3453
Fragments arranged by size:
3453 1071 876 85
BsmI GAATG_Cn'
Cuts at: 2617 2996 3089 2617
Size: 379 93 5013
Fragments arranged by size:
5013 379 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2170 2391 2607 2670 2804 3546
Size: 399 320 60 221 216 63 134 742
Cuts at: 3546 3631 4702 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 320 221 216 134
85 63 60
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 2586 2698 2840 844
Size: 1622 120 112 142 3489
Fragments arranged by size:
3489 1622 142 120 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 3372 1394
Size: 429 812 737 3507
Fragments arranged by size:
3507 812 737 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2299 2581 4129 4316 1700
Size: 110 489 282 1548 187 2869
Fragments arranged by size:
2869 1548 489 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 2112 2179 1925
Size: 187 67 5231
Fragments arranged by size:
5231 187 67
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 2713 794
Size: 1547 372 3566
Fragments arranged by size:
3566 1547 372
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480
Size: 843 367 385 17 1452 86 12 346
Cuts at: 5480 1972
Size: 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2359 513
Size: 552 939 6 349 3639
Fragments arranged by size:
3639 939 552 349 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2171 2301
Size: 54 1425 50 275 18 177 164 130
Cuts at: 2301 2362 2485 2932 3821 5450 8
Size: 61 123 447 889 1629 43
Fragments arranged by size:
1629 1425 889 447 275 177 164 130
123 61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2171 2485 2932 1537
Size: 275 359 314 447 4090
Fragments arranged by size:
4090 447 359 314 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 3415 1353
Size: 1292 770 3423
Fragments arranged by size:
3423 1292 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 2786 4072 4446 4592 387
Size: 1064 138 582 615 1286 374 146 1280
Fragments arranged by size:
1286 1280 1064 615 582 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 3548 4145 4932 5200 1239
Size: 393 656 1260 597 787 268 1524
Fragments arranged by size:
1524 1260 787 656 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2747 2438
Size: 309 5176
EcoO109I rG'GnC_Cy
Cuts at: 2100 2166 2280 2491 2532 2604 2776 2100
Size: 66 114 211 41 72 172 4809
Fragments arranged by size:
4809 211 172 114 72 66 41
EcoRI G'AATT_C
Cuts at: 2567 2794 3172 2567
Size: 227 378 4880
Fragments arranged by size:
4880 378 227
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2044 2237 2264 2300 2414 2696 4632 1678
Size: 366 193 27 36 114 282 1936 2531
Fragments arranged by size:
2531 1936 366 282 193 114 36 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373
Size: 32 135 32 2554 32 42 32 2626
Fragments arranged by size:
2626 2554 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2829 3075 669
Size: 988 281 442 449 246 3079
Fragments arranged by size:
3079 988 449 442 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2040 2233 2296 2410 2040
Size: 193 63 114 5115
Fragments arranged by size:
5115 193 114 63
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3663
Fragments arranged by size:
3663 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 3802 3961 518
Size: 648 2277 359 159 2042
Fragments arranged by size:
2277 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2186 2469 2614 2901 3578 4726 4971
Size: 406 174 283 145 287 677 1148 245
Cuts at: 4971 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 406 287 283 245 174
145
NaeI GCC'GGC
Cuts at: 2301 2583 2301
Size: 282 5203
NarI GG'CG_CC
Cuts at: 2041 2234 2297 2411 2041
Size: 193 63 114 5115
Fragments arranged by size:
5115 193 114 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3994
Fragments arranged by size:
3994 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NgoMIV G'CCGG_C
Cuts at: 2299 2581 2299
Size: 282 5203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2843 4388 1132
Size: 97 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5485
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 1108
Size: 1653 3832
PfoI T'CCnGG_A
Cuts at: 2121 2493 2121
Size: 372 5113
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2280 2491 2100
Size: 180 211 5094
Fragments arranged by size:
5094 211 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2106 2166 2603 2106
Size: 60 437 4988
Fragments arranged by size:
4988 437 60
PstI C_TGCA'G
Cuts at: 830 2144 2463 2837 830
Size: 1314 319 374 3478
Fragments arranged by size:
3478 1314 374 319
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
PvuII CAG'CTG
Cuts at: 2614 2614
Size: 5485
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SanDI GG'GwC_CC
Cuts at: 2280 2280
Size: 5485
SapI GCTCTTCn'nnn_
Cuts at: 2645 2645
Size: 5485
SbfI CC_TGCA'GG
Cuts at: 2144 2837 2144
Size: 693 4792
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2140 2459 2833 3968 4649 4840 826
Size: 219 1095 319 374 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 681 374 319 219 191
SfoI GGC'GCC
Cuts at: 2042 2235 2298 2412 2042
Size: 193 63 114 5115
Fragments arranged by size:
5115 193 114 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3589 4490 4752 820
Size: 197 926 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 1517
Size: 621 4864
StyI C'CwwG_G
Cuts at: 513 1065 2004 2924 513
Size: 552 939 920 3074
Fragments arranged by size:
3074 939 920 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 2512 3512 3529 3682 3867 4312
Size: 1255 909 23 1000 17 153 185 445
Cuts at: 4312 325
Size: 1498
Fragments arranged by size:
1498 1255 1000 909 445 185 153 23
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2652 3731 5443
Size: 274 80 33 51 1443 675 1079 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1443 1079 675 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SexAI SfiI SrfI SwaI XcmI