Restriction map for
pFN21AE2622
(Circular) (Six-base) MAPSORT of: pFN21AE2622.seq Check: 9062 from: 1 to: 5752
pFN21AE2622 5752 bp
With 182 enzymes: SgfI *
May 9, 2016 12:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2662 2721 3815 5199 5467 1632
Size: 1030 59 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1094 1030 268 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 5720
AlwNI CAG_nnn'CTG
Cuts at: 2282 2921 2960 3495 5067 2282
Size: 639 39 535 1572 2967
Fragments arranged by size:
2967 1572 639 535 39
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 5752
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 3061 3287 3439 5548 1493
Size: 1568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
BamHI G'GATC_C
Cuts at: 2357 2888 3082 3449 2357
Size: 531 194 367 4660
Fragments arranged by size:
4660 531 367 194
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2058 3071 1391
Size: 622 45 1013 4072
Fragments arranged by size:
4072 1013 622 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 2949 1548
Size: 1401 4351
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3041 4363 136
Size: 107 122 71 631 605 1369 1322 1525
Fragments arranged by size:
1525 1369 1322 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 2106 3169 2106
Size: 1063 4689
Bme1580I G_kGCm'C
Cuts at: 1790 2013 3813 4969 1790
Size: 223 1800 1156 2573
Fragments arranged by size:
2573 1800 1156 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2681 3483 4441
Size: 416 53 83 399 24 1264 802 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1264 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2437 4412 1239
Size: 1198 1975 2579
Fragments arranged by size:
2579 1975 1198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2428 4185 4583 4857 5004 5415 2428
Size: 1757 398 274 147 411 2765
Fragments arranged by size:
2765 1757 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 2662 4955 5451 2662
Size: 2293 496 2963
Fragments arranged by size:
2963 2293 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2058 3071 3813 3898 4969 1391
Size: 399 223 45 1013 742 85 1071 2174
Fragments arranged by size:
2174 1071 1013 742 399 223 85 45
BspEI T'CCGG_A
Cuts at: 2428 5415 2428
Size: 2987 2765
BspHI T'CATG_A
Cuts at: 3018 3641 5371 3018
Size: 623 1730 3399
Fragments arranged by size:
3399 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 2190 3812 4824 2190
Size: 1622 1012 3118
Fragments arranged by size:
3118 1622 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2357 2888 3082 3449 3834 3851 5303 5389
Size: 385 531 194 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 531 385 385 367 346 194
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2472 513
Size: 552 939 468 3793
Fragments arranged by size:
3793 939 552 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3268 4062 4082 2100
Size: 1168 794 20 3770
Fragments arranged by size:
3770 1168 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 2923 3033
Size: 54 1425 50 275 18 580 513 110
Cuts at: 3033 3199 4088 5717 8
Size: 166 889 1629 43
Fragments arranged by size:
1629 1425 889 580 513 275 166 110
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3682 1353
Size: 2329 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2662 2721 3815 4412 5199 5467
Size: 393 805 225 59 1094 597 787 268
Cuts at: 5467 1239
Size: 1524
Fragments arranged by size:
1524 1094 805 787 597 393 268 225
59
EcoO109I rG'GnC_Cy
Cuts at: 2009 2185 2279 2598 2636 2009
Size: 176 94 319 38 5125
Fragments arranged by size:
5125 319 176 94 38
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2342 2374 2590
Size: 32 135 32 453 32 285 32 216
Cuts at: 2590 2622 2994 3026 4393 4425 4467 4499 1373
Size: 32 372 32 1367 32 42 32 2626
Fragments arranged by size:
2626 1367 453 372 285 216 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 4866 5050 1305
Size: 1185 2376 184 2007
Fragments arranged by size:
2376 2007 1185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 3035 10
Size: 54 1425 343 580 623 2727
Fragments arranged by size:
2727 1425 623 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3710 4069 4228 518
Size: 648 2544 359 159 2042
Fragments arranged by size:
2544 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2886 3168 3845 4993 5238 1606
Size: 975 305 282 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 677 305 282 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2276 1348
Size: 928 4824
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2598 2636 2185
Size: 413 38 5301
Fragments arranged by size:
5301 413 38
PshAI GACnn'nnGTC
Cuts at: 1613 2939 1613
Size: 1326 4426
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 5752
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 5720
PstI C_TGCA'G
Cuts at: 830 2033 2143 2580 3104 830
Size: 1203 110 437 524 3478
Fragments arranged by size:
3478 1203 524 437 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2581 2581
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 3030 4000 5712 3030
Size: 970 1712 3070
Fragments arranged by size:
3070 1712 970
SfcI C'TryA_G
Cuts at: 826 1045 2029 2139 2576 3100 4235 4916 5107
Size: 219 984 110 437 524 1135 681 191
Cuts at: 5107 826
Size: 1471
Fragments arranged by size:
1471 1135 984 681 524 437 219 191
110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 3191 513
Size: 552 939 1187 3074
Fragments arranged by size:
3074 1187 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2971 3028 3998
Size: 274 80 33 51 1443 994 57 970
Cuts at: 3998 5710 96
Size: 1712 138
Fragments arranged by size:
1712 1443 994 970 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 4381
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI