Restriction map for
pFN21AE2619
(Circular) (Six-base) MAPSORT of: pFN21AE2619.seq Check: 7524 from: 1 to: 6073
pFN21AE2619 6073 bp
With 182 enzymes: SgfI *
April 15, 2016 11:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3416 1937
Size: 1479 4594
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4136 5520 5788 1632
Size: 2504 1384 268 1917
Fragments arranged by size:
2504 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 2134 3253 4972 5949 1203
Size: 931 1119 1719 977 1327
Fragments arranged by size:
1719 1327 1119 977 931
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 3816 5388 3816
Size: 1572 4501
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2317 2389 3074 3382 3608 3760 5869 1493
Size: 824 72 685 308 226 152 2109 1697
Fragments arranged by size:
2109 1697 824 685 308 226 152 72
AseI AT'TA_AT
Cuts at: 160 1032 2474 4627 160
Size: 872 1442 2153 1606
Fragments arranged by size:
2153 1606 1442 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 3398 1870
Size: 73 1455 4545
Fragments arranged by size:
4545 1455 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2295 2328 2295
Size: 33 6040
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3394 4849 618
Size: 325 217 627 1607 1455 1842
Fragments arranged by size:
1842 1607 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3392 1391
Size: 2001 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2456 2967 928
Size: 971 557 511 4034
Fragments arranged by size:
4034 971 557 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 2095 1548
Size: 547 5526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4684 136
Size: 107 122 71 631 605 3012 1525
Fragments arranged by size:
3012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2282 2786 6068 2282
Size: 504 3282 2287
Fragments arranged by size:
3282 2287 504
BlpI GC'TnA_GC
Cuts at: 2657 3490 2657
Size: 833 5240
Bme1580I G_kGCm'C
Cuts at: 1790 4134 5290 1790
Size: 2344 1156 2573
Fragments arranged by size:
2573 2344 1156
BmgBI CAC'GTC
Cuts at: 1318 3033 1318
Size: 1715 4358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3804 4762 442
Size: 416 53 83 399 24 2387 958 1753
Fragments arranged by size:
2387 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2803 4733 1239
Size: 1564 1930 2579
Fragments arranged by size:
2579 1930 1564
Bpu10I CC'TnA_GC
Cuts at: 2619 2619
Size: 6073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4736 882
Size: 617 3237 2219
Fragments arranged by size:
3237 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2135 3256 3864 493
Size: 711 931 1121 608 2702
Fragments arranged by size:
2702 1121 931 711 608
BsaBI GATnn'nnATC
Cuts at: 2158 2688 6067 2158
Size: 530 3379 2164
Fragments arranged by size:
3379 2164 530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4506 4904 5178 5325 5736 4506
Size: 398 274 147 411 4843
Fragments arranged by size:
4843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5848
Fragments arranged by size:
5848 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6073
BseYI C'CCAG_C
Cuts at: 5276 5772 5276
Size: 496 5577
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3523 4284 5312 1540
Size: 275 186 1135 387 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 387 275 186
BsiHKAI G_wGCw'C
Cuts at: 2875 4134 4219 5290 2875
Size: 1259 85 1071 3658
Fragments arranged by size:
3658 1259 1071 85
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2875 3392 4134 4219 5290 1391
Size: 399 1085 517 742 85 1071 2174
Fragments arranged by size:
2174 1085 1071 742 517 399 85
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 2680 3962 5692 2680
Size: 1282 1730 3061
Fragments arranged by size:
3061 1730 1282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4717 4904 1700
Size: 110 2907 187 2869
Fragments arranged by size:
2907 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstYI r'GATC_y
Cuts at: 1972 2282 2786 3403 3770 4155 4172 5624 5710
Size: 310 504 617 367 385 17 1452 86
Cuts at: 5710 5722 6068 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 617 504 385 367 346 310
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3354 3354
Size: 6073
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3056 3313 3589 4383 4403 2799
Size: 257 257 276 794 20 4469
Fragments arranged by size:
4469 794 276 257 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038
Size: 54 1425 50 275 18 1690 889 1629
Cuts at: 6038 8
Size: 43
Fragments arranged by size:
1690 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2164 4003 1353
Size: 811 1839 3423
Fragments arranged by size:
3423 1839 811
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2803 4136 4733 5520 5788 1239
Size: 393 1171 1333 597 787 268 1524
Fragments arranged by size:
1524 1333 1171 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2062 2062
Size: 6073
EcoO109I rG'GnC_Cy
Cuts at: 2928 2928
Size: 6073
EcoRI G'AATT_C
Cuts at: 3074 3382 3760 3074
Size: 308 378 5387
Fragments arranged by size:
5387 378 308
EcoRV GAT'ATC
Cuts at: 2276 2398 2650 2276
Size: 122 252 5699
Fragments arranged by size:
5699 252 122
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 5220 1678
Size: 3542 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2857 2889 2900
Size: 32 135 32 439 32 814 32 11
Cuts at: 2900 2932 4714 4746 4788 4820 1373
Size: 32 1782 32 42 32 2626
Fragments arranged by size:
2626 1782 814 439 135 42 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3417 3663 669
Size: 988 281 1479 246 3079
Fragments arranged by size:
3079 1479 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2440 2621 748
Size: 1692 181 4200
Fragments arranged by size:
4200 1692 181
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2359 3008 5187 5371 1305
Size: 1054 649 2179 184 2007
Fragments arranged by size:
2179 2007 1054 649 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4251
Fragments arranged by size:
4251 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2697 3114 4031 4390 4549 518
Size: 648 951 580 417 917 359 159 2042
Fragments arranged by size:
2042 951 917 648 580 417 359 159
MspA1I CmG'CkG
Cuts at: 1606 2672 3276 3489 4166 5314 5559 1606
Size: 1066 604 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 677 604 245 213
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 3431 4976 1132
Size: 97 2202 1545 2229
Fragments arranged by size:
2229 2202 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4444
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PmlI CAC'GTG
Cuts at: 3256 3256
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2928 2928
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 2895 1613
Size: 1282 4791
PsiI TTA'TAA
Cuts at: 2346 3643 2346
Size: 1297 4776
PstI C_TGCA'G
Cuts at: 830 2794 3291 3425 830
Size: 1964 497 134 3478
Fragments arranged by size:
3478 1964 497 134
PvuI CG_AT'CG
Cuts at: 2001 3136 2001
Size: 1135 4938
PvuII CAG'CTG
Cuts at: 2672 3276 2672
Size: 604 5469
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 2483 2766 4321 6033 2483
Size: 283 1555 1712 2523
Fragments arranged by size:
2523 1712 1555 283
SfcI C'TryA_G
Cuts at: 826 1045 2790 3287 3421 4556 5237 5428 826
Size: 219 1745 497 134 1135 681 191 1471
Fragments arranged by size:
1745 1471 1135 681 497 219 191 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4177 5078 5340 820
Size: 197 926 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2642 3512 513
Size: 552 939 30 608 870 3074
Fragments arranged by size:
3074 939 870 608 552 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2196 3104 4100 4117 4270 4455 4900
Size: 1255 616 908 996 17 153 185 445
Cuts at: 4900 325
Size: 1498
Fragments arranged by size:
1498 1255 996 908 616 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 2764 4319
Size: 274 80 33 51 1443 504 283 1555
Cuts at: 4319 6031 96
Size: 1712 138
Fragments arranged by size:
1712 1555 1443 504 283 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2683 3030 3245 2365
Size: 318 347 215 5193
Fragments arranged by size:
5193 347 318 215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 2173 4202 2173
Size: 2029 4044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI