Restriction map for
pFN21AE2613
(Circular) (Six-base) MAPSORT of: pFN21AE2613.seq Check: 4850 from: 1 to: 5935
pFN21AE2613 5935 bp
With 182 enzymes: SgfI *
March 25, 2016 16:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 3105 3256 3105
Size: 151 5784
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3998 5382 5650 1632
Size: 2366 1384 268 1917
Fragments arranged by size:
2366 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2933 1184
Size: 1749 4186
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 2057
Size: 32 5903
AlwNI CAG_nnn'CTG
Cuts at: 2095 2686 3102 3678 5250 2095
Size: 591 416 576 1572 2780
Fragments arranged by size:
2780 1572 591 576 416
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2262 2538 3260 1870
Size: 73 319 276 722 4545
Fragments arranged by size:
4545 722 319 276 73
BamHI G'GATC_C
Cuts at: 2106 3265 3632 2106
Size: 1159 367 4409
Fragments arranged by size:
4409 1159 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2325 3105 3148 3256
Size: 325 217 627 252 286 780 43 108
Cuts at: 3256 4711 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 780 627 325 286 252 217
108 43
BanII G_rGCy'C
Cuts at: 1391 2022 2166 2672 2844 3146 3254 1391
Size: 631 144 506 172 302 108 4072
Fragments arranged by size:
4072 631 506 302 172 144 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 2930 928
Size: 971 526 505 3933
Fragments arranged by size:
3933 971 526 505
BbvCI CC'TCA_GC
Cuts at: 3165 3171 3165
Size: 6 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BfrBI ATG'CAT
Cuts at: 2274 2274
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2653 2904 4546
Size: 107 122 71 631 605 981 251 1642
Cuts at: 4546 136
Size: 1525
Fragments arranged by size:
1642 1525 981 631 605 251 122 107
71
BglII A'GATC_T
Cuts at: 2544 5930 2544
Size: 3386 2549
BlpI GC'TnA_GC
Cuts at: 2558 2583 3352 2558
Size: 25 769 5141
Fragments arranged by size:
5141 769 25
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2475 3153 3996 5152 1790
Size: 314 371 678 843 1156 2573
Fragments arranged by size:
2573 1156 843 678 371 314
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 3666 4624
Size: 416 53 83 399 24 729 1520 958
Cuts at: 4624 442
Size: 1753
Fragments arranged by size:
1753 1520 958 729 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2152 2177 2186 2219 2660 2858 2875 3014
Size: 913 25 9 33 441 198 17 139
Cuts at: 3014 4595 1239
Size: 1581 2579
Fragments arranged by size:
2579 1581 913 441 198 139 33 25
17 9
Bpu10I CC'TnA_GC
Cuts at: 3165 3171 3165
Size: 6 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3197 4060 4925 5223 3197
Size: 863 865 298 3909
Fragments arranged by size:
3909 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4598 882
Size: 617 3099 2219
Fragments arranged by size:
3099 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 3726 493
Size: 711 1044 1478 2702
Fragments arranged by size:
2702 1478 1044 711
BsaBI GATnn'nnATC
Cuts at: 2520 5929 2520
Size: 3409 2526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5710
Fragments arranged by size:
5710 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2435 2438 2441 2946 2979 1186
Size: 988 261 3 3 505 33 4142
Fragments arranged by size:
4142 988 505 261 33 3 3
BseYI C'CCAG_C
Cuts at: 2130 2216 2456 5138 5634 2130
Size: 86 240 2682 496 2431
Fragments arranged by size:
2682 2431 496 240 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2770 1163
Size: 573 1034 4328
Fragments arranged by size:
4328 1034 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 3996 4081 5152 2166
Size: 1830 85 1071 2949
Fragments arranged by size:
2949 1830 1071 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2880 2880
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2104 2166 2475 2672 2844 3146
Size: 399 232 82 62 309 197 172 302
Cuts at: 3146 3153 3254 3996 4081 5152 1391
Size: 7 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 302 232 197
172 101 85 82 62 7
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 3290 844
Size: 2212 234 3489
Fragments arranged by size:
3489 2212 234
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 2963 3182 4430 4604 65
Size: 1183 936 779 219 1248 174 1396
Fragments arranged by size:
1396 1248 1183 936 779 219 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 4579 4766 1700
Size: 110 244 2525 187 2869
Fragments arranged by size:
2869 2525 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 2307 96
Size: 2211 3724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2186 794
Size: 1392 4543
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2389 2899 3116 2176
Size: 213 510 217 4995
Fragments arranged by size:
4995 510 217 213
BstYI r'GATC_y
Cuts at: 1972 2106 2544 3265 3632 4017 4034 5486 5572
Size: 134 438 721 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 721 438 385 367 346 134
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2511 513
Size: 552 939 507 3937
Fragments arranged by size:
3937 939 552 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 2734 3084 3451 4245 4265 2734
Size: 350 367 794 20 4404
Fragments arranged by size:
4404 794 367 350 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2189 4942 809
Size: 507 873 2753 1802
Fragments arranged by size:
2753 1802 873 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 2466 2754
Size: 54 1425 50 275 18 561 75 288
Cuts at: 2754 3382 4271 5900 8
Size: 628 889 1629 43
Fragments arranged by size:
1629 1425 889 628 561 288 275 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3003 3865 1353
Size: 1650 862 3423
Fragments arranged by size:
3423 1650 862
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2164 2164
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2177 2186 2219 2660 2858 2875
Size: 393 520 25 9 33 441 198 17
Cuts at: 2875 3014 3998 4595 5382 5650 1239
Size: 139 984 597 787 268 1524
Fragments arranged by size:
1524 984 787 597 520 441 393 268
198 139 33 25 17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2491 2491
Size: 5935
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 4576 4608 4650
Size: 32 135 32 712 32 2260 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 2260 712 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3109 3260 3109
Size: 151 5784
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 5049 5233 1305
Size: 1242 2502 184 2007
Fragments arranged by size:
2502 2007 1242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 10
Size: 54 1425 343 561 3552
Fragments arranged by size:
3552 1425 561 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 3114 3893 4252 4411 518
Size: 648 1221 727 779 359 159 2042
Fragments arranged by size:
2042 1221 779 727 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 2895 3351 4028 5176 5421 1606
Size: 1077 212 456 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 677 456 245 212
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 5935
NcoI C'CATG_G
Cuts at: 513 1065 2004 2511 513
Size: 552 939 507 3937
Fragments arranged by size:
3937 939 552 507
NdeI CA'TA_TG
Cuts at: 387 2719 387
Size: 2332 3603
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NsiI A_TGCA'T
Cuts at: 2276 2276
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2274 2354 3293 4838 1132
Size: 97 1045 80 939 1545 2229
Fragments arranged by size:
2229 1545 1045 939 97 80
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 4618
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2517 2686 3102 1108
Size: 1409 169 416 3941
Fragments arranged by size:
3941 1409 416 169
PfoI T'CCnGG_A
Cuts at: 2061 2835 2061
Size: 774 5161
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3287 830
Size: 2457 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2895 2895
Size: 5935
SacI G_AGCT'C
Cuts at: 2166 2166
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2149 3283 4418 5099 5290 826
Size: 219 1104 1134 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 1104 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2264 3262 1872
Size: 392 998 4545
Fragments arranged by size:
4545 998 392
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3212 4039 4940 5202 820
Size: 197 926 1269 827 901 262 1553
Fragments arranged by size:
1553 1269 926 901 827 262 197
SnaBI TAC'GTA
Cuts at: 493 2248 3726 493
Size: 1755 1478 2702
Fragments arranged by size:
2702 1755 1478
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 2274 3293 1132
Size: 1142 1019 3774
Fragments arranged by size:
3774 1142 1019
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 2511 2812 3137 3374 513
Size: 552 939 483 24 301 325 237 3074
Fragments arranged by size:
3074 939 552 483 325 301 237 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 4181 5893
Size: 274 80 33 51 1443 330 1874 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1874 1712 1443 330 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 2262 3260 1870
Size: 392 998 4545
Fragments arranged by size:
4545 998 392
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI