Restriction map for
pFN21AE2612
(Circular) (Six-base) MAPSORT of: pFN21AE2612.seq Check: 7994 from: 1 to: 5860
pFN21AE2612 5860 bp
With 182 enzymes: SgfI *
March 10, 2016 11:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 2887 2095
Size: 792 5068
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 2941 3171 3923 5307 5575 1632
Size: 1079 230 230 752 1384 268 1917
Fragments arranged by size:
1917 1384 1079 752 268 230 230
AfeI AGC'GCT
Cuts at: 2407 2407
Size: 5860
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2079 2664 4759 5736 1203
Size: 876 585 2095 977 1327
Fragments arranged by size:
2095 1327 977 876 585
AgeI A'CCGG_T
Cuts at: 2712 4691 2712
Size: 1979 3881
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 2556
Size: 32 5828
AlwNI CAG_nnn'CTG
Cuts at: 3603 5175 3603
Size: 1572 4288
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2720 3169 3395 3547 5656 1493
Size: 1227 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 449 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2481 3185 1870
Size: 73 120 418 704 4545
Fragments arranged by size:
4545 704 418 120 73
AvrII C'CTAG_G
Cuts at: 2507 2507
Size: 5860
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3026 3181 4636 618
Size: 325 217 627 1239 155 1455 1842
Fragments arranged by size:
1842 1455 1239 627 325 217 155
BanII G_rGCy'C
Cuts at: 1391 2322 2955 3179 1391
Size: 931 633 224 4072
Fragments arranged by size:
4072 931 633 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2690 3041 928
Size: 971 791 351 3747
Fragments arranged by size:
3747 971 791 351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3071 3277 3071
Size: 206 5654
Bme1580I G_kGCm'C
Cuts at: 1790 3921 5077 1790
Size: 2131 1156 2573
Fragments arranged by size:
2573 2131 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3093 3591 4549
Size: 416 53 83 399 24 1676 498 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1676 958 498 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2796 1056
Size: 1740 4120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2390 2422 4520 1239
Size: 1097 54 32 2098 2579
Fragments arranged by size:
2579 2098 1097 54 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2217 2876 3985 4850 5148 2217
Size: 659 1109 865 298 2929
Fragments arranged by size:
2929 1109 865 659 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2712 2744 2869 4293 4691 4965 5112 5523 2712
Size: 32 125 1424 398 274 147 411 3049
Fragments arranged by size:
3049 1424 411 398 274 147 125 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2741 2771 1178
Size: 30 165 30 1338 30 4267
Fragments arranged by size:
4267 1338 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2731 1186
Size: 895 650 4315
Fragments arranged by size:
4315 895 650
BseYI C'CCAG_C
Cuts at: 2395 2465 2819 5063 5559 2395
Size: 70 354 2244 496 2696
Fragments arranged by size:
2696 2244 496 354 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 4900
Fragments arranged by size:
4900 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2955 3921 4006 5077 2955
Size: 966 85 1071 3738
Fragments arranged by size:
3738 1071 966 85
BsmI GAATG_Cn'
Cuts at: 3025 3371 3464 3025
Size: 346 93 5421
Fragments arranged by size:
5421 346 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2955 3179 3921 4006 5077 1391
Size: 399 532 633 224 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 633 532 399 224 85
BspEI T'CCGG_A
Cuts at: 2744 5523 2744
Size: 2779 3081
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2887 3215 844
Size: 1251 792 328 3489
Fragments arranged by size:
3489 1251 792 328
BsrBI CCG'CTC
Cuts at: 1394 1823 2554 3747 1394
Size: 429 731 1193 3507
Fragments arranged by size:
3507 1193 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2933 4355 4529 65
Size: 1183 1685 1422 174 1396
Fragments arranged by size:
1685 1422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2712 2985 4504 4691 1700
Size: 110 902 273 1519 187 2869
Fragments arranged by size:
2869 1519 902 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 2122 1925
Size: 197 5663
BssSI C'ACGA_G
Cuts at: 2012 2099 3920 4932 2012
Size: 87 1821 1012 2940
Fragments arranged by size:
2940 1821 1012 87
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 2050 1184
Size: 866 4994
BstXI CCAn_nnnn'nTGG
Cuts at: 2314 3083 2314
Size: 769 5091
BstYI r'GATC_y
Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855
Size: 1218 367 385 17 1452 86 12 346
Cuts at: 5855 1972
Size: 1977
Fragments arranged by size:
1977 1452 1218 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2073 2073
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2160 2919 513
Size: 552 1095 759 3454
Fragments arranged by size:
3454 1095 759 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2961 487
Size: 683 56 1735 3386
Fragments arranged by size:
3386 1735 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2685 3376 4170 4190 2357
Size: 328 691 794 20 4027
Fragments arranged by size:
4027 794 691 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2625 3307
Size: 54 1425 50 275 18 510 285 682
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 510 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2960 3147 3790 1353
Size: 1607 187 643 3423
Fragments arranged by size:
3423 1607 643 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2953 2953
Size: 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2390 2422 2711 2941 3171 3923
Size: 393 704 54 32 289 230 230 752
Cuts at: 3923 4520 5307 5575 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 752 704 597 393 289 268
230 230 54 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2765 2102
Size: 663 5197
EcoO109I rG'GnC_Cy
Cuts at: 2770 2770
Size: 5860
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2789 2789
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2409 2590 5007 1678
Size: 731 181 2417 2531
Fragments arranged by size:
2531 2417 731 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2464 2496 4501
Size: 32 135 32 641 32 219 32 2005
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2005 641 219 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3204 3450 669
Size: 988 281 1143 123 246 3079
Fragments arranged by size:
3079 1143 988 281 246 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 2524 2925 3459 2524
Size: 401 534 4925
Fragments arranged by size:
4925 534 401
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 2627 10
Size: 54 1425 343 510 285 3243
Fragments arranged by size:
3243 1425 510 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2601 2621 2967 3818 4177 4336 518
Size: 648 1435 20 346 851 359 159 2042
Fragments arranged by size:
2042 1435 851 648 359 346 159 20
MspA1I CmG'CkG
Cuts at: 1606 2245 3276 3953 5101 5346 1606
Size: 639 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 677 639 245
NcoI C'CATG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3454
Fragments arranged by size:
3454 1854 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 2792 1052
Size: 1740 4120
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2083 2668 2738 3218 4763 1132
Size: 97 854 585 70 480 1545 2229
Fragments arranged by size:
2229 1545 854 585 480 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 2079 2664 4759 2079
Size: 585 2095 3180
Fragments arranged by size:
3180 2095 585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2703 2703
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2057 1613
Size: 444 5416
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5860
SacI G_AGCT'C
Cuts at: 2955 2955
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SapI GCTCTTCn'nnn_
Cuts at: 2960 2960
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 2756 4108 5820 2756
Size: 1352 1712 2796
Fragments arranged by size:
2796 1712 1352
SfcI C'TryA_G
Cuts at: 826 1045 3208 4343 5024 5215 826
Size: 219 2163 1135 681 191 1471
Fragments arranged by size:
2163 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2232 2891 3964 4865 5127 820
Size: 197 926 289 659 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 659 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 2439 1517
Size: 870 52 4938
Fragments arranged by size:
4938 870 52
StyI C'CwwG_G
Cuts at: 513 1065 2487 2507 2547 2835 2919 3299 513
Size: 552 1422 20 40 288 84 380 3074
Fragments arranged by size:
3074 1422 552 380 288 84 40 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 3887 3904 4057 4242 4687 325
Size: 1255 1122 1185 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1185 1122 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 4106 5818
Size: 274 80 33 51 1443 777 1352 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1352 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 5820 5852 2741
Size: 32 3047 32 2749
Fragments arranged by size:
3047 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2152 1316
Size: 836 5024
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2917 2167
Size: 750 5110
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI