Restriction map for
pFN21AE2606
(Circular) (Six-base) MAPSORT of: pFN21AE2606.seq Check: 4068 from: 1 to: 6823
pFN21AE2606 6823 bp
With 182 enzymes: SgfI *
February 12, 2016 18:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4166 1937
Size: 2229 4594
Acc65I G'GTAC_C
Cuts at: 3501 4144 3501
Size: 643 6180
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3958 4886 6270 6538 1632
Size: 2326 928 1384 268 1917
Fragments arranged by size:
2326 1917 1384 928 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 5722 6699 1203
Size: 4519 977 1327
Fragments arranged by size:
4519 1327 977
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 5552 5552
Size: 6823
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6823
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 6791
AlwNI CAG_nnn'CTG
Cuts at: 3204 3589 4566 6138 3204
Size: 385 977 1572 3889
Fragments arranged by size:
3889 1572 977 385
ApaI G_GGCC'C
Cuts at: 3328 3439 3328
Size: 111 6712
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2312 3021 3671 4132 4358 4510 6619 1493
Size: 819 709 650 461 226 152 2109 1697
Fragments arranged by size:
2109 1697 819 709 650 461 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 3569 4148 1870
Size: 73 448 1178 579 4545
Fragments arranged by size:
4545 1178 579 448 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3557 3590 3557
Size: 33 6790
BamHI G'GATC_C
Cuts at: 3846 4153 4520 3846
Size: 307 367 6149
Fragments arranged by size:
6149 367 307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2624 3501 3651 3713 4144
Size: 325 217 627 837 877 150 62 431
Cuts at: 4144 5599 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 877 837 627 431 325 217
150 62
BanII G_rGCy'C
Cuts at: 1391 3328 3439 4142 1391
Size: 1937 111 703 4072
Fragments arranged by size:
4072 1937 703 111
BbeI G_GCGC'C
Cuts at: 2628 2628
Size: 6823
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3145 4062 928
Size: 971 1246 917 3689
Fragments arranged by size:
3689 1246 971 917
BbvCI CC'TCA_GC
Cuts at: 2454 2454
Size: 6823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2984 3062 3886 4715 5925 2984
Size: 78 824 829 1210 3882
Fragments arranged by size:
3882 1210 829 824 78
BclI T'GATC_A
Cuts at: 1548 2796 2961 1548
Size: 1248 165 5410
Fragments arranged by size:
5410 1248 165
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 3137 5434
Size: 107 122 71 631 605 1387 78 2297
Cuts at: 5434 136
Size: 1525
Fragments arranged by size:
2297 1525 1387 631 605 122 107 78
71
BglII A'GATC_T
Cuts at: 2334 3186 3342 6818 2334
Size: 852 156 3476 2339
Fragments arranged by size:
3476 2339 852 156
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3328 3439 4884 6040 1790
Size: 450 1088 111 1445 1156 2573
Fragments arranged by size:
2573 1445 1156 1088 450 111
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3145 3442 3928
Size: 416 53 83 399 24 1728 297 486
Cuts at: 3928 4554 5512 442
Size: 626 958 1753
Fragments arranged by size:
1753 1728 958 626 486 416 399 297
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3677 4049 5483 1239
Size: 2438 372 1434 2579
Fragments arranged by size:
2579 2438 1434 372
Bpu10I CC'TnA_GC
Cuts at: 2454 3497 2454
Size: 1043 5780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4064 4948 5813 6111 4064
Size: 884 865 298 4776
Fragments arranged by size:
4776 884 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2172 3230 5486 882
Size: 617 673 1058 2256 2219
Fragments arranged by size:
2256 2219 1058 673 617
BsaAI yAC'GTr
Cuts at: 493 1204 2146 2370 4614 493
Size: 711 942 224 2244 2702
Fragments arranged by size:
2702 2244 942 711 224
BsaBI GATnn'nnATC
Cuts at: 2798 3704 6817 2798
Size: 906 3113 2804
Fragments arranged by size:
3113 2804 906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 4630 5012 5647
Size: 53 83 186 936 1092 2005 382 635
Cuts at: 5647 275
Size: 1451
Fragments arranged by size:
2005 1451 1092 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3012 5256 5654 5928 6075 6486 3012
Size: 2244 398 274 147 411 3349
Fragments arranged by size:
3349 2244 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3497 3527 1178
Size: 30 165 30 2094 30 4474
Fragments arranged by size:
4474 2094 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3238 1186
Size: 2052 4771
BseYI C'CCAG_C
Cuts at: 2495 2818 3331 3391 6026 6522 2495
Size: 323 513 60 2635 496 2796
Fragments arranged by size:
2796 2635 513 496 323 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2915 3569 3709 3908 1163
Size: 573 344 835 654 140 199 4078
Fragments arranged by size:
4078 835 654 573 344 199 140
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 4050 4273 5034 6062 1540
Size: 275 186 358 1691 223 761 1028 2301
Fragments arranged by size:
2301 1691 1028 761 358 275 223 186
BsiHKAI G_wGCw'C
Cuts at: 3083 4884 4969 6040 3083
Size: 1801 85 1071 3866
Fragments arranged by size:
3866 1801 1071 85
BsmI GAATG_Cn'
Cuts at: 2383 3691 3745 4334 4427 2383
Size: 1308 54 589 93 4779
Fragments arranged by size:
4779 1308 589 93 54
BsmBI CGTCTCn'nnnn_
Cuts at: 2561 2698 2561
Size: 137 6686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 3083 3328 3439 4142 4884 4969
Size: 399 450 843 245 111 703 742 85
Cuts at: 4969 6040 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 843 742 703 450 399 245
111 85
BspEI T'CCGG_A
Cuts at: 3012 6486 3012
Size: 3474 3349
BspHI T'CATG_A
Cuts at: 3624 3696 3756 4712 6442 3624
Size: 72 60 956 1730 4005
Fragments arranged by size:
4005 1730 956 72 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2937 3233 3465 4178 844
Size: 2093 296 232 713 3489
Fragments arranged by size:
3489 2093 713 296 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3472 4710 1394
Size: 429 194 1455 1238 3507
Fragments arranged by size:
3507 1455 1238 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 2569 2724 5318 5492 65
Size: 1183 1321 155 2594 174 1396
Fragments arranged by size:
2594 1396 1321 1183 174 155
BsrFI r'CCGG_y
Cuts at: 1700 1810 2873 3460 5467 5654 1700
Size: 110 1063 587 2007 187 2869
Fragments arranged by size:
2869 2007 1063 587 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6823
BssHII G'CGCG_C
Cuts at: 1925 3528 1925
Size: 1603 5220
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6823
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 3100 1184
Size: 1916 4907
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 2299
Size: 6823
BstYI r'GATC_y
Cuts at: 1972 2334 3186 3342 3846 4153 4520 4905 4922
Size: 362 852 156 504 307 367 385 17
Cuts at: 4922 6374 6460 6472 6818 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 852 504 385 367 362 346
307 156 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2730 513
Size: 552 939 726 4606
Fragments arranged by size:
4606 939 726 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2750 3013 3112 487
Size: 683 56 1524 263 99 4198
Fragments arranged by size:
4198 1524 683 263 99 56
BtsI GCAGTG_nn'
Cuts at: 2836 3144 4339 5133 5153 2836
Size: 308 1195 794 20 4506
Fragments arranged by size:
4506 1195 794 308 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 3372 3408
Size: 54 1425 50 275 18 300 1242 36
Cuts at: 3408 3487 3540 3732 4047 4270 5159 6788 8
Size: 79 53 192 315 223 889 1629 43
Fragments arranged by size:
1629 1425 1242 889 315 300 275 223
192 79 54 53 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 4047 4270 1537
Size: 275 2235 223 4090
Fragments arranged by size:
4090 2235 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 3011 3479 3812 4753 1353
Size: 1658 468 333 941 3423
Fragments arranged by size:
3423 1658 941 468 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5410 5784 5930 387
Size: 1064 138 3821 374 146 1280
Fragments arranged by size:
3821 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3677 3958 4049 4886 5483 6270 6538
Size: 393 2045 281 91 837 597 787 268
Cuts at: 6538 1239
Size: 1524
Fragments arranged by size:
2045 1524 837 787 597 393 281 268
91
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 3733 2618
Size: 1115 5708
EcoO109I rG'GnC_Cy
Cuts at: 2075 3325 3414 3436 4024 2075
Size: 1250 89 22 588 4874
Fragments arranged by size:
4874 1250 588 89 22
EcoRI G'AATT_C
Cuts at: 2312 3021 4132 4510 2312
Size: 709 1111 378 4625
Fragments arranged by size:
4625 1111 709 378
EcoRV GAT'ATC
Cuts at: 2104 2104
Size: 6823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4105 4105
Size: 6823
FspI TGC'GCA
Cuts at: 2672 3443 5329 2672
Size: 771 1886 4166
Fragments arranged by size:
4166 1886 771
FspAI rTGC'GCAy
Cuts at: 2672 2672
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2628 3392 3497 5970 1678
Size: 950 764 105 2473 2531
Fragments arranged by size:
2531 2473 950 764 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3765 3797 5464 5496 5538
Size: 32 135 32 2193 32 1667 32 42
Cuts at: 5538 5570 1373
Size: 32 2626
Fragments arranged by size:
2626 2193 1667 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4167 4413 669
Size: 988 281 2229 246 3079
Fragments arranged by size:
3079 2229 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KasI G'GCGC_C
Cuts at: 2624 2624
Size: 6823
KpnI G_GTAC'C
Cuts at: 3505 4148 3505
Size: 643 6180
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3192 5937 6121 1305
Size: 1887 2745 184 2007
Fragments arranged by size:
2745 2007 1887 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 3374 3542 10
Size: 54 1425 343 300 1242 168 3291
Fragments arranged by size:
3291 1425 1242 343 300 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 2735 2804 3560 3773 3992 4781
Size: 648 1563 6 69 756 213 219 789
Cuts at: 4781 5140 5299 518
Size: 359 159 2042
Fragments arranged by size:
2042 1563 789 756 648 359 219 213
159 69 6
MspA1I CmG'CkG
Cuts at: 1606 3586 4115 4239 4916 6064 6309 1606
Size: 1980 529 124 677 1148 245 2120
Fragments arranged by size:
2120 1980 1148 677 529 245 124
NaeI GCC'GGC
Cuts at: 2875 3462 2875
Size: 587 6236
NarI GG'CG_CC
Cuts at: 2625 2625
Size: 6823
NcoI C'CATG_G
Cuts at: 513 1065 2004 2730 513
Size: 552 939 726 4606
Fragments arranged by size:
4606 939 726 552
NdeI CA'TA_TG
Cuts at: 387 2108 387
Size: 1721 5102
NgoMIV G'CCGG_C
Cuts at: 2873 3460 2873
Size: 587 6236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NruI TCG'CGA
Cuts at: 3032 3032
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 3991 4181 5726 1132
Size: 97 2762 190 1545 2229
Fragments arranged by size:
2762 2229 1545 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 5722 5722
Size: 6823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6823
PfoI T'CCnGG_A
Cuts at: 2594 3783 2594
Size: 1189 5634
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 4024 2075
Size: 1949 4874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PspOMI G'GGCC_C
Cuts at: 3324 3435 3324
Size: 111 6712
PspXI vC'TCGA_Gb
Cuts at: 3569 3569
Size: 6823
PstI C_TGCA'G
Cuts at: 830 3342 3601 4175 830
Size: 2512 259 574 3478
Fragments arranged by size:
3478 2512 574 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SapI GCTCTTCn'nnn_
Cuts at: 3479 3479
Size: 6823
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 2405 2852 5071 6783 2405
Size: 447 2219 1712 2445
Fragments arranged by size:
2445 2219 1712 447
SexAI A'CCwGG_T
Cuts at: 2613 2945 2613
Size: 332 6491
SfcI C'TryA_G
Cuts at: 826 1045 2151 3338 3597 4171 5306 5987 6178
Size: 219 1106 1187 259 574 1135 681 191
Cuts at: 6178 826
Size: 1471
Fragments arranged by size:
1471 1187 1135 1106 681 574 259 219
191
SfoI GGC'GCC
Cuts at: 2626 2626
Size: 6823
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 2393 4150 1872
Size: 521 1757 4545
Fragments arranged by size:
4545 1757 521
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3569 4079 4927 5828 6090 820
Size: 197 926 1626 510 848 901 262 1553
Fragments arranged by size:
1626 1553 926 901 848 510 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 2832 4747 5
Size: 47 2780 1915 2081
Fragments arranged by size:
2780 2081 1915 47
StuI AGG'CCT
Cuts at: 1517 2718 4057 1517
Size: 1201 1339 4283
Fragments arranged by size:
4283 1339 1201
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 2730 4262 513
Size: 552 939 501 225 1532 3074
Fragments arranged by size:
3074 1532 939 552 501 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 3884 4850 4867 5020 5205 5650
Size: 1255 1199 1105 966 17 153 185 445
Cuts at: 5650 325
Size: 1498
Fragments arranged by size:
1498 1255 1199 1105 966 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 2850 5069
Size: 274 80 33 51 1443 426 447 2219
Cuts at: 5069 6781 96
Size: 1712 138
Fragments arranged by size:
2219 1712 1443 447 426 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 6783 6815 3497
Size: 32 3254 32 3505
Fragments arranged by size:
3505 3254 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2503 3061 3382 2503
Size: 558 321 5944
Fragments arranged by size:
5944 558 321
XhoI C'TCGA_G
Cuts at: 1943 3569 1943
Size: 1626 5197
XmaI C'CCGG_G
Cuts at: 1870 2391 4148 1870
Size: 521 1757 4545
Fragments arranged by size:
4545 1757 521
XmnI GAAnn'nnTTC
Cuts at: 3743 4952 3743
Size: 1209 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoICRI FseI NsiI PacI PmlI PsrI PvuII
RsrII SacI SacII SanDI SfiI SrfI SwaI