(Circular) (Six-base) MAPSORT of: pFN21AE2606.seq Check: 4068 from: 1 to: 6823 pFN21AE2606 6823 bp With 182 enzymes: SgfI * February 12, 2016 18:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4166 1937 Size: 2229 4594 Acc65I G'GTAC_C Cuts at: 3501 4144 3501 Size: 643 6180 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3958 4886 6270 6538 1632 Size: 2326 928 1384 268 1917 Fragments arranged by size: 2326 1917 1384 928 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 5722 6699 1203 Size: 4519 977 1327 Fragments arranged by size: 4519 1327 977 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 5552 5552 Size: 6823 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6823 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3989 4021 3989 Size: 32 6791 AlwNI CAG_nnn'CTG Cuts at: 3204 3589 4566 6138 3204 Size: 385 977 1572 3889 Fragments arranged by size: 3889 1572 977 385 ApaI G_GGCC'C Cuts at: 3328 3439 3328 Size: 111 6712 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2312 3021 3671 4132 4358 4510 6619 1493 Size: 819 709 650 461 226 152 2109 1697 Fragments arranged by size: 2109 1697 819 709 650 461 226 152 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 2391 3569 4148 1870 Size: 73 448 1178 579 4545 Fragments arranged by size: 4545 1178 579 448 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3557 3590 3557 Size: 33 6790 BamHI G'GATC_C Cuts at: 3846 4153 4520 3846 Size: 307 367 6149 Fragments arranged by size: 6149 367 307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2624 3501 3651 3713 4144 Size: 325 217 627 837 877 150 62 431 Cuts at: 4144 5599 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 877 837 627 431 325 217 150 62 BanII G_rGCy'C Cuts at: 1391 3328 3439 4142 1391 Size: 1937 111 703 4072 Fragments arranged by size: 4072 1937 703 111 BbeI G_GCGC'C Cuts at: 2628 2628 Size: 6823 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3145 4062 928 Size: 971 1246 917 3689 Fragments arranged by size: 3689 1246 971 917 BbvCI CC'TCA_GC Cuts at: 2454 2454 Size: 6823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2984 3062 3886 4715 5925 2984 Size: 78 824 829 1210 3882 Fragments arranged by size: 3882 1210 829 824 78 BclI T'GATC_A Cuts at: 1548 2796 2961 1548 Size: 1248 165 5410 Fragments arranged by size: 5410 1248 165 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3059 3137 5434 Size: 107 122 71 631 605 1387 78 2297 Cuts at: 5434 136 Size: 1525 Fragments arranged by size: 2297 1525 1387 631 605 122 107 78 71 BglII A'GATC_T Cuts at: 2334 3186 3342 6818 2334 Size: 852 156 3476 2339 Fragments arranged by size: 3476 2339 852 156 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 2240 3328 3439 4884 6040 1790 Size: 450 1088 111 1445 1156 2573 Fragments arranged by size: 2573 1445 1156 1088 450 111 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3145 3442 3928 Size: 416 53 83 399 24 1728 297 486 Cuts at: 3928 4554 5512 442 Size: 626 958 1753 Fragments arranged by size: 1753 1728 958 626 486 416 399 297 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3677 4049 5483 1239 Size: 2438 372 1434 2579 Fragments arranged by size: 2579 2438 1434 372 Bpu10I CC'TnA_GC Cuts at: 2454 3497 2454 Size: 1043 5780 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4064 4948 5813 6111 4064 Size: 884 865 298 4776 Fragments arranged by size: 4776 884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2172 3230 5486 882 Size: 617 673 1058 2256 2219 Fragments arranged by size: 2256 2219 1058 673 617 BsaAI yAC'GTr Cuts at: 493 1204 2146 2370 4614 493 Size: 711 942 224 2244 2702 Fragments arranged by size: 2702 2244 942 711 224 BsaBI GATnn'nnATC Cuts at: 2798 3704 6817 2798 Size: 906 3113 2804 Fragments arranged by size: 3113 2804 906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2625 4630 5012 5647 Size: 53 83 186 936 1092 2005 382 635 Cuts at: 5647 275 Size: 1451 Fragments arranged by size: 2005 1451 1092 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3012 5256 5654 5928 6075 6486 3012 Size: 2244 398 274 147 411 3349 Fragments arranged by size: 3349 2244 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3497 3527 1178 Size: 30 165 30 2094 30 4474 Fragments arranged by size: 4474 2094 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3238 1186 Size: 2052 4771 BseYI C'CCAG_C Cuts at: 2495 2818 3331 3391 6026 6522 2495 Size: 323 513 60 2635 496 2796 Fragments arranged by size: 2796 2635 513 496 323 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 2915 3569 3709 3908 1163 Size: 573 344 835 654 140 199 4078 Fragments arranged by size: 4078 835 654 573 344 199 140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2359 4050 4273 5034 6062 1540 Size: 275 186 358 1691 223 761 1028 2301 Fragments arranged by size: 2301 1691 1028 761 358 275 223 186 BsiHKAI G_wGCw'C Cuts at: 3083 4884 4969 6040 3083 Size: 1801 85 1071 3866 Fragments arranged by size: 3866 1801 1071 85 BsmI GAATG_Cn' Cuts at: 2383 3691 3745 4334 4427 2383 Size: 1308 54 589 93 4779 Fragments arranged by size: 4779 1308 589 93 54 BsmBI CGTCTCn'nnnn_ Cuts at: 2561 2698 2561 Size: 137 6686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 3083 3328 3439 4142 4884 4969 Size: 399 450 843 245 111 703 742 85 Cuts at: 4969 6040 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 843 742 703 450 399 245 111 85 BspEI T'CCGG_A Cuts at: 3012 6486 3012 Size: 3474 3349 BspHI T'CATG_A Cuts at: 3624 3696 3756 4712 6442 3624 Size: 72 60 956 1730 4005 Fragments arranged by size: 4005 1730 956 72 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2937 3233 3465 4178 844 Size: 2093 296 232 713 3489 Fragments arranged by size: 3489 2093 713 296 232 BsrBI CCG'CTC Cuts at: 1394 1823 2017 3472 4710 1394 Size: 429 194 1455 1238 3507 Fragments arranged by size: 3507 1455 1238 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 2569 2724 5318 5492 65 Size: 1183 1321 155 2594 174 1396 Fragments arranged by size: 2594 1396 1321 1183 174 155 BsrFI r'CCGG_y Cuts at: 1700 1810 2873 3460 5467 5654 1700 Size: 110 1063 587 2007 187 2869 Fragments arranged by size: 2869 2007 1063 587 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6823 BssHII G'CGCG_C Cuts at: 1925 3528 1925 Size: 1603 5220 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6823 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 3100 1184 Size: 1916 4907 BstXI CCAn_nnnn'nTGG Cuts at: 2299 2299 Size: 6823 BstYI r'GATC_y Cuts at: 1972 2334 3186 3342 3846 4153 4520 4905 4922 Size: 362 852 156 504 307 367 385 17 Cuts at: 4922 6374 6460 6472 6818 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 852 504 385 367 362 346 307 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2730 513 Size: 552 939 726 4606 Fragments arranged by size: 4606 939 726 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2750 3013 3112 487 Size: 683 56 1524 263 99 4198 Fragments arranged by size: 4198 1524 683 263 99 56 BtsI GCAGTG_nn' Cuts at: 2836 3144 4339 5133 5153 2836 Size: 308 1195 794 20 4506 Fragments arranged by size: 4506 1195 794 308 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 3372 3408 Size: 54 1425 50 275 18 300 1242 36 Cuts at: 3408 3487 3540 3732 4047 4270 5159 6788 8 Size: 79 53 192 315 223 889 1629 43 Fragments arranged by size: 1629 1425 1242 889 315 300 275 223 192 79 54 53 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4047 4270 1537 Size: 275 2235 223 4090 Fragments arranged by size: 4090 2235 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 3011 3479 3812 4753 1353 Size: 1658 468 333 941 3423 Fragments arranged by size: 3423 1658 941 468 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5410 5784 5930 387 Size: 1064 138 3821 374 146 1280 Fragments arranged by size: 3821 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3677 3958 4049 4886 5483 6270 6538 Size: 393 2045 281 91 837 597 787 268 Cuts at: 6538 1239 Size: 1524 Fragments arranged by size: 2045 1524 837 787 597 393 281 268 91 EcoNI CCTnn'n_nnAGG Cuts at: 2618 3733 2618 Size: 1115 5708 EcoO109I rG'GnC_Cy Cuts at: 2075 3325 3414 3436 4024 2075 Size: 1250 89 22 588 4874 Fragments arranged by size: 4874 1250 588 89 22 EcoRI G'AATT_C Cuts at: 2312 3021 4132 4510 2312 Size: 709 1111 378 4625 Fragments arranged by size: 4625 1111 709 378 EcoRV GAT'ATC Cuts at: 2104 2104 Size: 6823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4105 4105 Size: 6823 FspI TGC'GCA Cuts at: 2672 3443 5329 2672 Size: 771 1886 4166 Fragments arranged by size: 4166 1886 771 FspAI rTGC'GCAy Cuts at: 2672 2672 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2628 3392 3497 5970 1678 Size: 950 764 105 2473 2531 Fragments arranged by size: 2531 2473 950 764 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3765 3797 5464 5496 5538 Size: 32 135 32 2193 32 1667 32 42 Cuts at: 5538 5570 1373 Size: 32 2626 Fragments arranged by size: 2626 2193 1667 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4167 4413 669 Size: 988 281 2229 246 3079 Fragments arranged by size: 3079 2229 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KasI G'GCGC_C Cuts at: 2624 2624 Size: 6823 KpnI G_GTAC'C Cuts at: 3505 4148 3505 Size: 643 6180 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3192 5937 6121 1305 Size: 1887 2745 184 2007 Fragments arranged by size: 2745 2007 1887 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 3374 3542 10 Size: 54 1425 343 300 1242 168 3291 Fragments arranged by size: 3291 1425 1242 343 300 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2729 2735 2804 3560 3773 3992 4781 Size: 648 1563 6 69 756 213 219 789 Cuts at: 4781 5140 5299 518 Size: 359 159 2042 Fragments arranged by size: 2042 1563 789 756 648 359 219 213 159 69 6 MspA1I CmG'CkG Cuts at: 1606 3586 4115 4239 4916 6064 6309 1606 Size: 1980 529 124 677 1148 245 2120 Fragments arranged by size: 2120 1980 1148 677 529 245 124 NaeI GCC'GGC Cuts at: 2875 3462 2875 Size: 587 6236 NarI GG'CG_CC Cuts at: 2625 2625 Size: 6823 NcoI C'CATG_G Cuts at: 513 1065 2004 2730 513 Size: 552 939 726 4606 Fragments arranged by size: 4606 939 726 552 NdeI CA'TA_TG Cuts at: 387 2108 387 Size: 1721 5102 NgoMIV G'CCGG_C Cuts at: 2873 3460 2873 Size: 587 6236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NruI TCG'CGA Cuts at: 3032 3032 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 3991 4181 5726 1132 Size: 97 2762 190 1545 2229 Fragments arranged by size: 2762 2229 1545 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 5722 5722 Size: 6823 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6823 PfoI T'CCnGG_A Cuts at: 2594 3783 2594 Size: 1189 5634 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2075 4024 2075 Size: 1949 4874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PspOMI G'GGCC_C Cuts at: 3324 3435 3324 Size: 111 6712 PspXI vC'TCGA_Gb Cuts at: 3569 3569 Size: 6823 PstI C_TGCA'G Cuts at: 830 3342 3601 4175 830 Size: 2512 259 574 3478 Fragments arranged by size: 3478 2512 574 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SapI GCTCTTCn'nnn_ Cuts at: 3479 3479 Size: 6823 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 2405 2852 5071 6783 2405 Size: 447 2219 1712 2445 Fragments arranged by size: 2445 2219 1712 447 SexAI A'CCwGG_T Cuts at: 2613 2945 2613 Size: 332 6491 SfcI C'TryA_G Cuts at: 826 1045 2151 3338 3597 4171 5306 5987 6178 Size: 219 1106 1187 259 574 1135 681 191 Cuts at: 6178 826 Size: 1471 Fragments arranged by size: 1471 1187 1135 1106 681 574 259 219 191 SfoI GGC'GCC Cuts at: 2626 2626 Size: 6823 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 2393 4150 1872 Size: 521 1757 4545 Fragments arranged by size: 4545 1757 521 SmlI C'TyrA_G Cuts at: 820 1017 1943 3569 4079 4927 5828 6090 820 Size: 197 926 1626 510 848 901 262 1553 Fragments arranged by size: 1626 1553 926 901 848 510 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 2832 4747 5 Size: 47 2780 1915 2081 Fragments arranged by size: 2780 2081 1915 47 StuI AGG'CCT Cuts at: 1517 2718 4057 1517 Size: 1201 1339 4283 Fragments arranged by size: 4283 1339 1201 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 2730 4262 513 Size: 552 939 501 225 1532 3074 Fragments arranged by size: 3074 1532 939 552 501 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 3884 4850 4867 5020 5205 5650 Size: 1255 1199 1105 966 17 153 185 445 Cuts at: 5650 325 Size: 1498 Fragments arranged by size: 1498 1255 1199 1105 966 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 2850 5069 Size: 274 80 33 51 1443 426 447 2219 Cuts at: 5069 6781 96 Size: 1712 138 Fragments arranged by size: 2219 1712 1443 447 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3497 3529 6783 6815 3497 Size: 32 3254 32 3505 Fragments arranged by size: 3505 3254 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2503 3061 3382 2503 Size: 558 321 5944 Fragments arranged by size: 5944 558 321 XhoI C'TCGA_G Cuts at: 1943 3569 1943 Size: 1626 5197 XmaI C'CCGG_G Cuts at: 1870 2391 4148 1870 Size: 521 1757 4545 Fragments arranged by size: 4545 1757 521 XmnI GAAnn'nnTTC Cuts at: 3743 4952 3743 Size: 1209 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI NsiI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI