Restriction map for
pFN21AE2600
(Circular) (Six-base) MAPSORT of: pFN21AE2600.seq Check: 5252 from: 1 to: 5596
pFN21AE2600 5596 bp
With 182 enzymes: SgfI *
January 12, 2016 11:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 3659 5043 5311 1632
Size: 1082 945 1384 268 1917
Fragments arranged by size:
1917 1384 1082 945 268
AfeI AGC'GCT
Cuts at: 2264 2576 2264
Size: 312 5284
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2593 4325 2593
Size: 1732 3864
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 5564
AlwNI CAG_nnn'CTG
Cuts at: 2323 3339 4911 2323
Size: 1016 1572 3008
Fragments arranged by size:
3008 1572 1016
ApaLI G'TGCA_C
Cuts at: 2142 2870 3653 4809 2142
Size: 728 783 1156 2929
Fragments arranged by size:
2929 1156 783 728
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2921 1870
Size: 73 152 826 4545
Fragments arranged by size:
4545 826 152 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4372 618
Size: 325 217 627 1130 1455 1842
Fragments arranged by size:
1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2343 2477 2915 1391
Size: 705 247 134 438 4072
Fragments arranged by size:
4072 705 438 247 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2682 928
Size: 971 555 228 3842
Fragments arranged by size:
3842 971 555 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2123 2157 3787 3821 1903
Size: 34 50 34 102 34 1630 34 3678
Fragments arranged by size:
3678 1630 102 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2874 3657 4813 1790
Size: 356 728 783 1156 2573
Fragments arranged by size:
2573 1156 783 728 356
BmgBI CAC'GTC
Cuts at: 1318 2220 2869 1318
Size: 902 649 4045
Fragments arranged by size:
4045 902 649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 3327 4285
Size: 416 53 83 399 24 729 1181 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1181 958 729 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2470 4256 1239
Size: 1149 82 1786 2579
Fragments arranged by size:
2579 1786 1149 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 2540 5590 2540
Size: 3050 2546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 2609 2639 1178
Size: 30 165 30 1059 30 117 30 4135
Fragments arranged by size:
4135 1059 165 117 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2614 1186
Size: 1428 4168
BseYI C'CCAG_C
Cuts at: 4799 5295 4799
Size: 496 5100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5023
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2312 2335 3046 3807 4835 1540
Size: 275 186 311 23 711 761 1028 2301
Fragments arranged by size:
2301 1028 761 711 311 275 186 23
BsiHKAI G_wGCw'C
Cuts at: 2096 2146 2606 2874 3657 3742 4813 2096
Size: 50 460 268 783 85 1071 2879
Fragments arranged by size:
2879 1071 783 460 268 85 50
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
BsmBI CGTCTCn'nnnn_
Cuts at: 2488 2488
Size: 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2146 2343 2477 2606 2874 2915
Size: 399 306 50 197 134 129 268 41
Cuts at: 2915 3657 3742 4813 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 268 197 134
129 85 50 41
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2951 844
Size: 1547 560 3489
Fragments arranged by size:
3489 1547 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2459 4240 4427 1700
Size: 110 261 388 1781 187 2869
Fragments arranged by size:
2869 1781 388 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 2725 96
Size: 2629 2967
BssHII G'CGCG_C
Cuts at: 1925 2157 2374 1925
Size: 232 217 5147
Fragments arranged by size:
5147 232 217
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4067
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2062 2112 2335 513
Size: 552 997 50 223 3774
Fragments arranged by size:
3774 997 552 223 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2470 2777 2872 3112 3906 3926 2470
Size: 307 95 240 794 20 4140
Fragments arranged by size:
4140 794 307 240 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DraIII CAC_nnn'GTG
Cuts at: 2500 2500
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 3043 3932
Size: 54 1425 50 275 18 502 711 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 711 502 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2332 3043 1537
Size: 275 520 711 4090
Fragments arranged by size:
4090 711 520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2154 4183 4557 4703 387
Size: 1064 138 565 2029 374 146 1280
Fragments arranged by size:
2029 1280 1064 565 374 146 138
EcoICRI GAG'CTC
Cuts at: 2094 2094
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2470 2714 3659 4256 5043 5311
Size: 393 756 82 244 945 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 945 787 756 597 393 268 244
82
EcoNI CCTnn'n_nnAGG
Cuts at: 2784 2838 2784
Size: 54 5542
EcoO109I rG'GnC_Cy
Cuts at: 2108 2108
Size: 5596
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 5596
FspI TGC'GCA
Cuts at: 2224 4102 2224
Size: 1878 3718
FspAI rTGC'GCAy
Cuts at: 2224 2224
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2266 2324 2396 2460 2578 4743 1678
Size: 588 58 72 64 118 2165 2531
Fragments arranged by size:
2531 2165 588 118 72 64 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2462 2494 2579
Size: 32 135 32 438 32 420 32 85
Cuts at: 2579 2611 4237 4269 4311 4343 1373
Size: 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1626 438 420 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2587 2940 3186 669
Size: 988 281 412 237 353 246 3079
Fragments arranged by size:
3079 988 412 353 281 246 237
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 4710 4894 1305
Size: 1116 2289 184 2007
Fragments arranged by size:
2289 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3554 3913 4072 518
Size: 648 2388 359 159 2042
Fragments arranged by size:
2388 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2036 2064 2314 2337 3012 3689 4837
Size: 405 25 28 250 23 675 677 1148
Cuts at: 4837 5082 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 675 405 250 245 28
25 23
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 5596
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2561 2887 2954 4499 1132
Size: 97 1332 326 67 1545 2229
Fragments arranged by size:
2229 1545 1332 326 97 67
PasI CC'CwG_GG
Cuts at: 1348 2718 1348
Size: 1370 4226
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 1108
Size: 920 4676
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 3665 3697 5178 5210 2609
Size: 32 1024 32 1481 32 2995
Fragments arranged by size:
2995 1481 1024 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2108 2108
Size: 5596
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 2760 3166 2760
Size: 406 5190
PstI C_TGCA'G
Cuts at: 830 2319 2948 830
Size: 1489 629 3478
Fragments arranged by size:
3478 1489 629
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SacI G_AGCT'C
Cuts at: 2096 2096
Size: 5596
SacII CC_GC'GG
Cuts at: 2065 2338 2065
Size: 273 5323
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SanDI GG'GwC_CC
Cuts at: 2108 2108
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2315 2878 2944 4079 4760 4951 826
Size: 219 1270 563 66 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 563 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 2071 1700
Size: 371 5225
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2561 2887 2954 1132
Size: 1429 326 67 3774
Fragments arranged by size:
3774 1429 326 67
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2853 3035 513
Size: 552 1788 182 3074
Fragments arranged by size:
3074 1788 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2725 3842 5554
Size: 274 80 33 51 1443 748 1117 1712
Cuts at: 5554 96
Size: 138
Fragments arranged by size:
1712 1443 1117 748 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI
SrfI SwaI XcmI