Restriction map for
pFN21AE2594
(Circular) (Six-base) MAPSORT of: pFN21AE2594.seq Check: 9840 from: 1 to: 15697
pFN21AE2594 15697 bp
With 182 enzymes: SgfI *
January 6, 2016 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5270 8747 5270
Size: 3477 12220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 13507 278
Size: 53 83 186 936 2668 9303 2468
Fragments arranged by size:
9303 2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7104 9539 13040 1937
Size: 5167 2435 3501 4594
Fragments arranged by size:
5167 4594 3501 2435
Acc65I G'GTAC_C
Cuts at: 13018 13018
Size: 15697
AclI AA'CG_TT
Cuts at: 1563 12843 13824 14197 1563
Size: 11280 981 373 3063
Fragments arranged by size:
11280 3063 981 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2397 3087 3108 4494 4739 5490 6024
Size: 522 243 690 21 1386 245 751 534
Cuts at: 6024 7431 7802 10458 10907 12063 12530 12648 13760
Size: 1407 371 2656 449 1156 467 118 1112
Cuts at: 13760 15144 15412 1632
Size: 1384 268 1917
Fragments arranged by size:
2656 1917 1407 1386 1384 1156 1112 751
690 534 522 467 449 371 268 245
243 118 21
AfeI AGC'GCT
Cuts at: 3383 4355 11352 3383
Size: 972 6997 7728
Fragments arranged by size:
7728 6997 972
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 15500
AflIII A'CryG_T
Cuts at: 1203 5067 8030 8460 11608 14596 15573 1203
Size: 3864 2963 430 3148 2988 977 1327
Fragments arranged by size:
3864 3148 2988 2963 1327 977 430
AgeI A'CCGG_T
Cuts at: 9319 11922 14528 9319
Size: 2603 2606 10488
Fragments arranged by size:
10488 2606 2603
AhdI GACnn_n'nnGTC
Cuts at: 14426 14426
Size: 15697
AleI CACnn'nnGTG
Cuts at: 1166 4688 8051 10133 1166
Size: 3522 3363 2082 6730
Fragments arranged by size:
6730 3522 3363 2082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 11210 12124 12173 1184
Size: 10026 914 49 4708
Fragments arranged by size:
10026 4708 914 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4381 4413 5767 5799 4381
Size: 32 1354 32 14279
Fragments arranged by size:
14279 1354 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2776 2951 6869 7262 7352 7409 7439 8770 9686
Size: 175 3918 393 90 57 30 1331 916
Cuts at: 9686 11519 11845 12538 12566 13440 15012 2776
Size: 1833 326 693 28 874 1572 3461
Fragments arranged by size:
3918 3461 1833 1572 1331 916 874 693
393 326 175 90 57 30 28
ApaI G_GGCC'C
Cuts at: 8060 10071 11019 11404 12035 12639 8060
Size: 2011 948 385 631 604 11118
Fragments arranged by size:
11118 2011 948 631 604 385
ApaLI G'TGCA_C
Cuts at: 2178 8760 11891 13754 14910 2178
Size: 6582 3131 1863 1156 2965
Fragments arranged by size:
6582 3131 2965 1863 1156
ApoI r'AATT_y
Cuts at: 1493 2112 3077 3086 3266 3296 5133 5927 5998
Size: 619 965 9 180 30 1837 794 71
Cuts at: 5998 6293 6421 9232 9901 10541 12434 13006 13232
Size: 295 128 2811 669 640 1893 572 226
Cuts at: 13232 13384 15493 1493
Size: 152 2109 1697
Fragments arranged by size:
2811 2109 1893 1837 1697 965 794 669
640 619 572 295 226 180 152 128
71 30 9
AseI AT'TA_AT
Cuts at: 160 1032 14251 160
Size: 872 13219 1606
Fragments arranged by size:
13219 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15697
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2257 2268 2523 2679 3960 4035
Size: 73 102 212 11 255 156 1281 75
Cuts at: 4035 4647 7217 7578 10739 11011 11096 11364 11471
Size: 612 2570 361 3161 272 85 268 107
Cuts at: 11471 11505 13022 1870
Size: 34 1517 4545
Fragments arranged by size:
4545 3161 2570 1517 1281 612 361 272
268 255 212 156 107 102 85 75
73 34 11
AvrII C'CTAG_G
Cuts at: 2612 5840 2612
Size: 3228 12469
BamHI G'GATC_C
Cuts at: 5824 9468 13027 13394 5824
Size: 3644 3559 367 8127
Fragments arranged by size:
8127 3644 3559 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 6136 6997 7704 8997
Size: 325 217 627 462 3887 861 707 1293
Cuts at: 8997 11659 13018 14473 618
Size: 2662 1359 1455 1842
Fragments arranged by size:
3887 2662 1842 1455 1359 1293 861 707
627 462 325 217
BanII G_rGCy'C
Cuts at: 1391 2550 2620 2997 6774 7773 8060 8084 9485
Size: 1159 70 377 3777 999 287 24 1401
Cuts at: 9485 9535 10028 10071 10409 10746 11019 11371 11404
Size: 50 493 43 338 337 273 352 33
Cuts at: 11404 12035 12054 12639 13016 1391
Size: 631 19 585 377 4072
Fragments arranged by size:
4072 3777 1401 1159 999 631 585 493
377 377 352 338 337 287 273 70
50 43 33 24 19
BbeI G_GCGC'C
Cuts at: 7001 7708 9001 7001
Size: 707 1293 13697
Fragments arranged by size:
13697 1293 707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4500 5391 5402 6246 6977 7025 8463
Size: 971 2601 891 11 844 731 48 1438
Cuts at: 8463 10478 928
Size: 2015 6147
Fragments arranged by size:
6147 2601 2015 1438 971 891 844 731
48 11
BbvCI CC'TCA_GC
Cuts at: 5093 8103 8550 8878 11682 12066 5093
Size: 3010 447 328 2804 384 8724
Fragments arranged by size:
8724 3010 2804 447 384 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3597 3631 5473 5507 13888 13922 1903
Size: 34 1660 34 1842 34 8381 34 3678
Fragments arranged by size:
8381 3678 1842 1660 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 11819 13589 14799 11819
Size: 1770 1210 12717
Fragments arranged by size:
12717 1770 1210
BclI T'GATC_A
Cuts at: 1548 5365 1548
Size: 3817 11880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8576 11675 12824
Size: 107 122 71 631 605 6904 3099 1149
Cuts at: 12824 14308 136
Size: 1484 1525
Fragments arranged by size:
6904 3099 1525 1484 1149 631 605 122
107 71
BglII A'GATC_T
Cuts at: 6118 8240 8740 12885 15692 6118
Size: 2122 500 4145 2807 6123
Fragments arranged by size:
6123 4145 2807 2122 500
BlpI GC'TnA_GC
Cuts at: 7353 13114 7353
Size: 5761 9936
Bme1580I G_kGCm'C
Cuts at: 1790 2182 8060 8764 9288 10071 10154 11019 11199
Size: 392 5878 704 524 783 83 865 180
Cuts at: 11199 11404 11895 12035 12271 12639 13758 14914 1790
Size: 205 491 140 236 368 1119 1156 2573
Fragments arranged by size:
5878 2573 1156 1119 865 783 704 524
491 392 368 236 205 180 140 83
BmgBI CAC'GTC
Cuts at: 1318 5068 8033 8461 1318
Size: 3750 2965 428 8554
Fragments arranged by size:
8554 3750 2965 428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2634 2743 2959
Size: 416 53 83 399 24 1217 109 216
Cuts at: 2959 5276 5784 8404 8488 11186 11194 11316 11820
Size: 2317 508 2620 84 2698 8 122 504
Cuts at: 11820 13428 14386 442
Size: 1608 958 1753
Fragments arranged by size:
2698 2620 2317 1753 1608 1217 958 508
504 416 399 216 122 109 84 83
53 24 8
BmtI G_CTAG'C
Cuts at: 1056 2784 3468 1056
Size: 1728 684 13285
Fragments arranged by size:
13285 1728 684
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2283 2283
Size: 15697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2824 2904 3903 4236 4958 7416 8236 8537
Size: 1585 80 999 333 722 2458 820 301
Cuts at: 8537 8745 8841 9534 9580 9753 9822 10116 11194
Size: 208 96 693 46 173 69 294 1078
Cuts at: 11194 11822 11881 12689 14357 1239
Size: 628 59 808 1668 2579
Fragments arranged by size:
2579 2458 1668 1585 1078 999 820 808
722 693 628 333 301 294 208 173
96 80 69 59 46
Bpu10I CC'TnA_GC
Cuts at: 4902 5093 8103 8550 8878 9209 10408 11273 11682
Size: 191 3010 447 328 331 1199 865 409
Cuts at: 11682 12027 12066 12243 4902
Size: 345 39 177 8356
Fragments arranged by size:
8356 3010 1199 865 447 409 345 331
328 191 177 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 4880 5221 5634 7544 7913 9075 9321 10602
Size: 1374 341 413 1910 369 1162 246 1281
Cuts at: 10602 12147 12533 12773 13822 14687 14985 3506
Size: 1545 386 240 1049 865 298 4218
Fragments arranged by size:
4218 1910 1545 1374 1281 1162 1049 865
413 386 369 341 298 246 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2214 3710 4143 4765 5868 10904 12478
Size: 617 715 1496 433 622 1103 5036 1574
Cuts at: 12478 14360 882
Size: 1882 2219
Fragments arranged by size:
5036 2219 1882 1574 1496 1103 715 622
617 433
BsaAI yAC'GTr
Cuts at: 493 1204 6251 12877 13488 493
Size: 711 5047 6626 611 2702
Fragments arranged by size:
6626 5047 2702 711 611
BsaBI GATnn'nnATC
Cuts at: 5823 15691 5823
Size: 9868 5829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 6998 7338 7705
Size: 53 83 186 936 2668 2797 340 367
Cuts at: 7705 8998 13504 13886 14521 275
Size: 1293 4506 382 635 1451
Fragments arranged by size:
4506 2797 2668 1451 1293 936 635 382
367 340 186 83 53
BsaWI w'CCGG_w
Cuts at: 4287 4399 9319 11922 14130 14528 14802 14949 15360
Size: 112 4920 2603 2208 398 274 147 411
Cuts at: 15360 4287
Size: 4624
Fragments arranged by size:
4920 4624 2603 2208 411 398 274 147
112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2557 2587 3381 3411 9532
Size: 30 165 30 1154 30 794 30 6121
Cuts at: 9532 9562 9578 9608 9751 9781 10773 10803 12842
Size: 30 16 30 143 30 992 30 2039
Cuts at: 12842 12872 1178
Size: 30 4003
Fragments arranged by size:
6121 4003 2039 1154 992 794 165 143
30 30 30 30 30 30 30 30
30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2154 2343 2757 4174 4276 5661 7566 7907
Size: 968 189 414 1417 102 1385 1905 341
Cuts at: 7907 7910 8120 8123 9174 9177 9344 9536 9924
Size: 3 210 3 1051 3 167 192 388
Cuts at: 9924 11070 11352 11489 12027 12054 1186
Size: 1146 282 137 538 27 4829
Fragments arranged by size:
4829 1905 1417 1385 1146 1051 968 538
414 388 341 282 210 192 189 167
137 102 27 3 3 3
BseYI C'CCAG_C
Cuts at: 3526 5243 7272 7402 8704 9309 9389 10064 10976
Size: 1717 2029 130 1302 605 80 675 912
Cuts at: 10976 11018 11026 11129 11382 11434 11460 11958 12093
Size: 42 8 103 253 52 26 498 135
Cuts at: 12093 12120 12217 12398 14900 15396 3526
Size: 27 97 181 2502 496 3827
Fragments arranged by size:
3827 2502 2029 1717 1302 912 675 605
498 496 253 181 135 130 103 97
80 52 42 27 26 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2937 3128 5701 8607 10215 10236 11433
Size: 573 1201 191 2573 2906 1608 21 1197
Cuts at: 11433 11543 11546 11903 12084 12095 12590 1163
Size: 110 3 357 181 11 495 4270
Fragments arranged by size:
4270 2906 2573 1608 1201 1197 573 495
357 191 181 110 21 11 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7952 10390 13147 13908 14936 1540
Size: 275 186 5951 2438 2757 761 1028 2301
Fragments arranged by size:
5951 2757 2438 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2182 6774 8084 8764 10058 10452 11895 13758 13843
Size: 4592 1310 680 1294 394 1443 1863 85
Cuts at: 13843 14914 2182
Size: 1071 2965
Fragments arranged by size:
4592 2965 1863 1443 1310 1294 1071 680
394 85
BsmI GAATG_Cn'
Cuts at: 9355 10006 10165 10207 13208 13301 9355
Size: 651 159 42 3001 93 11751
Fragments arranged by size:
11751 3001 651 159 93 42
BsmBI CGTCTCn'nnnn_
Cuts at: 4227 4414 7045 9988 12589 4227
Size: 187 2631 2943 2601 7335
Fragments arranged by size:
7335 2943 2631 2601 187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2550 2620 2997 6774 7773 8060
Size: 399 392 368 70 377 3777 999 287
Cuts at: 8060 8084 8764 9288 9485 9535 10028 10058 10071
Size: 24 680 524 197 50 493 30 13
Cuts at: 10071 10154 10409 10452 10746 11019 11199 11371 11404
Size: 83 255 43 294 273 180 172 33
Cuts at: 11404 11895 12035 12054 12271 12639 13016 13758 13843
Size: 491 140 19 217 368 377 742 85
Cuts at: 13843 14914 1391
Size: 1071 2174
Fragments arranged by size:
3777 2174 1071 999 742 680 524 493
491 399 392 377 377 368 368 294
287 273 255 217 197 180 172 140
85 83 70 50 43 33 30 24
19 13
BspEI T'CCGG_A
Cuts at: 4399 15360 4399
Size: 10961 4736
BspHI T'CATG_A
Cuts at: 2593 12975 13586 15316 2593
Size: 10382 611 1730 2974
Fragments arranged by size:
10382 2974 1730 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5270 5344 7078 7116 7924 8747 12235 12819
Size: 4426 74 1734 38 808 823 3488 584
Cuts at: 12819 13052 844
Size: 233 3489
Fragments arranged by size:
4426 3489 3488 1734 823 808 584 233
74 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 2530 2707 7755 13584 1394
Size: 429 572 135 177 5048 5829 3507
Fragments arranged by size:
5829 5048 3507 572 429 177 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 9675 9843 10536 12984 14192 14366 65
Size: 1183 8427 168 693 2448 1208 174 1396
Fragments arranged by size:
8427 2448 1396 1208 1183 693 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 7875 8203 8882 9319 10927 11922 14341
Size: 110 6065 328 679 437 1608 995 2419
Cuts at: 14341 14528 1700
Size: 187 2869
Fragments arranged by size:
6065 2869 2419 1608 995 679 437 328
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 15697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 15697
BssSI C'ACGA_G
Cuts at: 2376 5630 6375 13757 14769 2376
Size: 3254 745 7382 1012 3304
Fragments arranged by size:
7382 3304 3254 1012 745
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2951 7058 10202 10304 10634 11890 12538 794
Size: 2157 4107 3144 102 330 1256 648 3953
Fragments arranged by size:
4107 3953 3144 2157 1256 648 330 102
BstBI TT'CG_AA
Cuts at: 3581 3913 6100 6427 14501 3581
Size: 332 2187 327 8074 4777
Fragments arranged by size:
8074 4777 2187 332 327
BstEII G'GTnAC_C
Cuts at: 1184 2733 7178 7739 9379 11869 1184
Size: 1549 4445 561 1640 2490 5012
Fragments arranged by size:
5012 4445 2490 1640 1549 561
BstXI CCAn_nnnn'nTGG
Cuts at: 3386 8053 8509 10531 3386
Size: 4667 456 2022 8552
Fragments arranged by size:
8552 4667 2022 456
BstYI r'GATC_y
Cuts at: 1972 2129 2518 2834 4933 5824 6118 6681 7252
Size: 157 389 316 2099 891 294 563 571
Cuts at: 7252 8240 8740 9468 10512 12885 13027 13394 13779
Size: 988 500 728 1044 2373 142 367 385
Cuts at: 13779 13796 15248 15334 15346 15692 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2373 2099 1977 1452 1044 988 891 728
571 563 500 389 385 367 346 316
294 157 142 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3933 7938 8037 9605 3933
Size: 4005 99 1568 10025
Fragments arranged by size:
10025 4005 1568 99
BtgI C'CryG_G
Cuts at: 513 1065 2004 7734 8085 8526 9527 10143 11579
Size: 552 939 5730 351 441 1001 616 1436
Cuts at: 11579 11640 11875 12489 12993 513
Size: 61 235 614 504 3217
Fragments arranged by size:
5730 3217 1436 1001 939 616 614 552
504 441 351 235 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 8521 10578 487
Size: 683 56 7295 2057 5606
Fragments arranged by size:
7295 5606 2057 683 56
BtsI GCAGTG_nn'
Cuts at: 2633 2884 2893 3793 7341 7506 8268 8926 9416
Size: 251 9 900 3548 165 762 658 490
Cuts at: 9416 10203 10224 10305 13213 14007 14027 2633
Size: 787 21 81 2908 794 20 4303
Fragments arranged by size:
4303 3548 2908 900 794 787 762 658
490 251 165 81 21 20 9
ClaI AT'CG_AT
Cuts at: 1554 5493 1554
Size: 3939 11758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9658 9693 529
Size: 35 9094 35 6533
Fragments arranged by size:
9094 6533 35 35
DraI TTT'AAA
Cuts at: 4983 5925 13001 4983
Size: 942 7076 7679
Fragments arranged by size:
7679 7076 942
DraIII CAC_nnn'GTG
Cuts at: 8938 11411 8938
Size: 2473 13224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12335 14704 809
Size: 507 11019 2369 1802
Fragments arranged by size:
11019 2369 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 5973 8655
Size: 54 1425 50 275 18 262 3881 2682
Cuts at: 8655 10266 10521 10644 10929 11342 12136 12748 13144
Size: 1611 255 123 285 413 794 612 396
Cuts at: 13144 14033 15662 8
Size: 889 1629 43
Fragments arranged by size:
3881 2682 1629 1611 1425 889 794 612
413 396 285 275 262 255 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 13144 1537
Size: 275 11332 4090
Fragments arranged by size:
11332 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 3123 3847 5910 6373 6552 7008 7526
Size: 702 1068 724 2063 463 179 456 518
Cuts at: 7526 7884 9561 12348 13627 1353
Size: 358 1677 2787 1279 3423
Fragments arranged by size:
3423 2787 2063 1677 1279 1068 724 702
518 463 456 358 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 14284 14658 14804 387
Size: 1064 138 12695 374 146 1280
Fragments arranged by size:
12695 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6772 8082 6772
Size: 1310 14387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2397 2824 2904 3087 3108 3903
Size: 393 522 243 427 80 183 21 795
Cuts at: 3903 4236 4494 4739 4958 5490 6024 7416 7431
Size: 333 258 245 219 532 534 1392 15
Cuts at: 7431 7802 8236 8537 8745 8841 9534 9580 9753
Size: 371 434 301 208 96 693 46 173
Cuts at: 9753 9822 10116 10458 10907 11194 11822 11881 12063
Size: 69 294 342 449 287 628 59 182
Cuts at: 12063 12530 12648 12689 13760 14357 15144 15412 1239
Size: 467 118 41 1071 597 787 268 1524
Fragments arranged by size:
1524 1392 1071 795 787 693 628 597
534 532 522 467 449 434 427 393
371 342 333 301 294 287 268 258
245 243 219 208 183 182 173 118
96 80 69 59 46 41 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 9710 11285 12042 9710
Size: 1575 757 13365
Fragments arranged by size:
13365 1575 757
EcoO109I rG'GnC_Cy
Cuts at: 2342 2805 7221 7959 8057 8593 9715 10013 10088
Size: 463 4416 738 98 536 1122 298 75
Cuts at: 10088 10613 10918 11015 11400 11401 11677 12031 12032
Size: 525 305 97 385 1 276 354 1
Cuts at: 12032 12186 12535 12635 12636 2342
Size: 154 349 100 1 5403
Fragments arranged by size:
5403 4416 1122 738 536 525 463 385
354 349 305 298 276 154 100 98
97 75 1 1 1
EcoRI G'AATT_C
Cuts at: 5998 9232 12434 13006 13384 5998
Size: 3234 3202 572 378 8311
Fragments arranged by size:
8311 3234 3202 572 378
EcoRV GAT'ATC
Cuts at: 2659 5713 2659
Size: 3054 12643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3083 3218 4727 4892 5153 6362 7656 8993 9669
Size: 135 1509 165 261 1209 1294 1337 676
Cuts at: 9669 9934 12814 3083
Size: 265 2880 5966
Fragments arranged by size:
5966 2880 1509 1337 1294 1209 676 265
261 165 135
FspI TGC'GCA
Cuts at: 14203 14203
Size: 15697
HaeII r_GCGC'y
Cuts at: 1678 3385 4357 4966 7001 7708 8771 9001 10500
Size: 1707 972 609 2035 707 1063 230 1499
Cuts at: 10500 11153 11354 14844 1678
Size: 653 201 3490 2531
Fragments arranged by size:
3490 2531 2035 1707 1499 1063 972 707
653 609 230 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3124 3156 5057
Size: 32 135 32 985 32 535 32 1901
Cuts at: 5057 5089 5607 5639 6392 6424 8327 8359 8387
Size: 32 518 32 753 32 1903 32 28
Cuts at: 8387 8419 8795 8827 9221 9253 9578 9610 10372
Size: 32 376 32 394 32 325 32 762
Cuts at: 10372 10391 10404 10423 10709 10741 12010 12042 14338
Size: 19 13 19 286 32 1269 32 2296
Cuts at: 14338 14370 14412 14444 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2296 1903 1901 1269 985 762 753
535 518 394 376 325 286 135 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
28 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 6454 9586 9733 10314 10999
Size: 988 281 265 4251 3132 147 581 685
Cuts at: 10999 13041 13287 669
Size: 2042 246 3079
Fragments arranged by size:
4251 3132 3079 2042 988 685 581 281
265 246 147
HindIII A'AGCT_T
Cuts at: 748 4709 6339 7595 10880 748
Size: 3961 1630 1256 3285 5565
Fragments arranged by size:
5565 3961 3285 1630 1256
HpaI GTT'AAC
Cuts at: 13287 13287
Size: 15697
KasI G'GCGC_C
Cuts at: 6997 7704 8997 6997
Size: 707 1293 13697
Fragments arranged by size:
13697 1293 707
KpnI G_GTAC'C
Cuts at: 13022 13022
Size: 15697
MfeI C'AATT_G
Cuts at: 13296 13296
Size: 15697
MluI A'CGCG_T
Cuts at: 15573 15573
Size: 15697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5821 7235 9105 9293 12981 14811 14995 1305
Size: 4516 1414 1870 188 3688 1830 184 2007
Fragments arranged by size:
4516 3688 2007 1870 1830 1414 188 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5975 10646 10
Size: 54 1425 343 4143 4671 5061
Fragments arranged by size:
5061 4671 4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4688 5817 6779 8051 10133 10376 11171
Size: 648 3522 1129 962 1272 2082 243 795
Cuts at: 11171 11192 11607 13655 14014 14173 518
Size: 21 415 2048 359 159 2042
Fragments arranged by size:
3522 2082 2048 2042 1272 1129 962 795
648 415 359 243 159 21
MspA1I CmG'CkG
Cuts at: 1606 2820 3707 3781 5350 7093 7496 7832 7976
Size: 1214 887 74 1569 1743 403 336 144
Cuts at: 7976 10187 10232 10489 10843 11057 11374 11438 11642
Size: 2211 45 257 354 214 317 64 204
Cuts at: 11642 11901 12023 12221 12563 12682 13113 13790 14938
Size: 259 122 198 342 119 431 677 1148
Cuts at: 14938 15183 1606
Size: 245 2120
Fragments arranged by size:
2211 2120 1743 1569 1214 1148 887 677
431 403 354 342 336 317 259 257
245 214 204 198 144 122 119 74
64 45
NaeI GCC'GGC
Cuts at: 7877 8884 10929 7877
Size: 1007 2045 12645
Fragments arranged by size:
12645 2045 1007
NarI GG'CG_CC
Cuts at: 6998 7705 8998 6998
Size: 707 1293 13697
Fragments arranged by size:
13697 1293 707
NcoI C'CATG_G
Cuts at: 513 1065 2004 7734 8085 11875 513
Size: 552 939 5730 351 3790 4335
Fragments arranged by size:
5730 4335 3790 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 15697
NgoMIV G'CCGG_C
Cuts at: 7875 8882 10927 7875
Size: 1007 2045 12645
Fragments arranged by size:
12645 2045 1007
NheI G'CTAG_C
Cuts at: 1052 2780 3464 1052
Size: 1728 684 13285
Fragments arranged by size:
13285 1728 684
NotI GC'GGCC_GC
Cuts at: 13144 13144
Size: 15697
NruI TCG'CGA
Cuts at: 2622 2622
Size: 15697
NspI r_CATG'y
Cuts at: 1132 1229 3217 3547 11612 13055 14600 1132
Size: 97 1988 330 8065 1443 1545 2229
Fragments arranged by size:
8065 2229 1988 1545 1443 330 97
PasI CC'CwG_GG
Cuts at: 1348 8310 10364 10777 10804 11287 11634 1348
Size: 6962 2054 413 27 483 347 5411
Fragments arranged by size:
6962 5411 2054 483 413 347 27
PciI A'CATG_T
Cuts at: 11608 14596 11608
Size: 2988 12709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 12528 1108
Size: 11420 4277
PfoI T'CCnGG_A
Cuts at: 2010 4213 8722 8820 12008 2010
Size: 2203 4509 98 3188 5699
Fragments arranged by size:
5699 4509 3188 2203 98
PmeI GTTT'AAAC
Cuts at: 13001 13001
Size: 15697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13766 13798 15279 15311 13766
Size: 32 1481 32 14152
Fragments arranged by size:
14152 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2805 7959 10918 12186 2805
Size: 5154 2959 1268 6316
Fragments arranged by size:
6316 5154 2959 1268
PshAI GACnn'nnGTC
Cuts at: 1613 10389 10916 11618 1613
Size: 8776 527 702 5692
Fragments arranged by size:
8776 5692 702 527
PsiI TTA'TAA
Cuts at: 2238 13267 2238
Size: 11029 4668
PspOMI G'GGCC_C
Cuts at: 8056 10067 11015 11400 12031 12635 8056
Size: 2011 948 385 631 604 11118
Fragments arranged by size:
11118 2011 948 631 604 385
PspXI vC'TCGA_Gb
Cuts at: 3960 4647 11505 3960
Size: 687 6858 8152
Fragments arranged by size:
8152 6858 687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3411 3443 6281 6313 3411
Size: 32 2838 32 12795
Fragments arranged by size:
12795 2838 32 32
PstI C_TGCA'G
Cuts at: 830 2944 4915 5355 7348 7501 7660 7831 7921
Size: 2114 1971 440 1993 153 159 171 90
Cuts at: 7921 7975 8323 8775 9436 10630 11185 11379 12060
Size: 54 348 452 661 1194 555 194 681
Cuts at: 12060 12816 12885 13049 830
Size: 756 69 164 3478
Fragments arranged by size:
3478 2114 1993 1971 1194 756 681 661
555 452 440 348 194 171 164 159
153 90 69 54
PvuI CG_AT'CG
Cuts at: 2001 10390 2001
Size: 8389 7308
PvuII CAG'CTG
Cuts at: 3707 3781 5350 7093 7496 7976 10843 11057 11438
Size: 74 1569 1743 403 480 2867 214 381
Cuts at: 11438 12023 12221 12563 12682 3707
Size: 585 198 342 119 6722
Fragments arranged by size:
6722 2867 1743 1569 585 480 403 381
342 214 198 119 74
SacI G_AGCT'C
Cuts at: 6774 8084 6774
Size: 1310 14387
SacII CC_GC'GG
Cuts at: 11643 11643
Size: 15697
SalI G'TCGA_C
Cuts at: 1936 13039 1936
Size: 11103 4594
SanDI GG'GwC_CC
Cuts at: 12186 12186
Size: 15697
SapI GCTCTTCn'nnn_
Cuts at: 6552 9561 6552
Size: 3009 12688
SbfI CC_TGCA'GG
Cuts at: 10630 12816 13049 10630
Size: 2186 233 13278
Fragments arranged by size:
13278 2186 233
ScaI AGT'ACT
Cuts at: 11137 13945 15657 11137
Size: 2808 1712 11177
Fragments arranged by size:
11177 2808 1712
SexAI A'CCwGG_T
Cuts at: 6127 6127
Size: 15697
SfcI C'TryA_G
Cuts at: 826 1045 2512 2662 2940 3042 4300 4911 5351
Size: 219 1467 150 278 102 1258 611 440
Cuts at: 5351 7344 7497 7656 7827 7917 7971 8319 8771
Size: 1993 153 159 171 90 54 348 452
Cuts at: 8771 8838 9057 9432 10626 10989 11181 11332 11375
Size: 67 219 375 1194 363 192 151 43
Cuts at: 11375 12056 12812 12881 13045 14180 14861 15052 826
Size: 681 756 69 164 1135 681 191 1471
Fragments arranged by size:
1993 1471 1467 1258 1194 1135 756 681
681 611 452 440 375 363 348 278
219 219 192 191 171 164 159 153
151 150 102 90 69 67 54 43
SfoI GGC'GCC
Cuts at: 6999 7706 8999 6999
Size: 707 1293 13697
Fragments arranged by size:
13697 1293 707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15697
SgrAI Cr'CCGG_yG
Cuts at: 1700 7875 9319 1700
Size: 6175 1444 8078
Fragments arranged by size:
8078 6175 1444
SmaI CCC'GGG
Cuts at: 1872 2681 7219 11098 13024 1872
Size: 809 4538 3879 1926 4545
Fragments arranged by size:
4545 4538 3879 1926 809
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2523 3521 3960 4647 4895 5236
Size: 197 926 580 998 439 687 248 341
Cuts at: 5236 5649 7559 7928 9090 9336 10617 11505 12162
Size: 413 1910 369 1162 246 1281 888 657
Cuts at: 12162 12548 12788 13801 14702 14964 820
Size: 386 240 1013 901 262 1553
Fragments arranged by size:
1910 1553 1281 1162 1013 998 926 901
888 687 657 580 439 413 386 369
341 262 248 246 240 197
SnaBI TAC'GTA
Cuts at: 493 13488 493
Size: 12995 2702
SpeI A'CTAG_T
Cuts at: 152 11536 152
Size: 11384 4313
SphI G_CATG'C
Cuts at: 1132 3217 3547 13055 1132
Size: 2085 330 9508 3774
Fragments arranged by size:
9508 3774 2085 330
SspI AAT'ATT
Cuts at: 5 52 13621 5
Size: 47 13569 2081
Fragments arranged by size:
13569 2081 47
StuI AGG'CCT
Cuts at: 1517 7861 8555 8854 9575 1517
Size: 6344 694 299 721 7639
Fragments arranged by size:
7639 6344 721 694 299
StyI C'CwwG_G
Cuts at: 513 1065 2004 2612 3276 3997 5840 7734 7908
Size: 552 939 608 664 721 1843 1894 174
Cuts at: 7908 7955 7990 8085 8094 8898 9918 10728 10914
Size: 47 35 95 9 804 1020 810 186
Cuts at: 10914 11114 11875 13136 513
Size: 200 761 1261 3074
Fragments arranged by size:
3074 1894 1843 1261 1020 939 810 804
761 721 664 608 552 200 186 174
95 47 35 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2020 2188 2201 2721 2971 6196 7727
Size: 1255 440 168 13 520 250 3225 1531
Cuts at: 7727 8507 9403 10377 10490 10664 10790 10817 11235
Size: 780 896 974 113 174 126 27 418
Cuts at: 11235 11273 12116 12269 12600 12794 13724 13741 13894
Size: 38 843 153 331 194 930 17 153
Cuts at: 13894 14079 14524 325
Size: 185 445 1498
Fragments arranged by size:
3225 1531 1498 1255 974 930 896 843
780 520 445 440 418 331 250 194
185 174 168 153 153 126 113 38
27 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 9127 9197 11135
Size: 274 80 33 51 1443 7150 70 1938
Cuts at: 11135 13943 15655 96
Size: 2808 1712 138
Fragments arranged by size:
7150 2808 1938 1712 1443 274 138 80
70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3381 3413 3721 3753 10352 10384 10773 10805 11962
Size: 32 308 32 6599 32 389 32 1157
Cuts at: 11962 11994 12461 12493 15657 15689 3381
Size: 32 467 32 3164 32 3389
Fragments arranged by size:
6599 3389 3164 1157 467 389 308 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4724 10892 11266 12781 1316
Size: 3408 6168 374 1515 4232
Fragments arranged by size:
6168 4232 3408 1515 374
XbaI T'CTAG_A
Cuts at: 2753 2792 2825 5075 6640 6943 13033 2753
Size: 39 33 2250 1565 303 6090 5417
Fragments arranged by size:
6090 5417 2250 1565 303 39 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7801 7941 8050 8260 8393 11173 12584 7801
Size: 140 109 210 133 2780 1411 10914
Fragments arranged by size:
10914 2780 1411 210 140 133 109
XhoI C'TCGA_G
Cuts at: 1943 2523 3960 4647 11505 1943
Size: 580 1437 687 6858 6135
Fragments arranged by size:
6858 6135 1437 687 580
XmaI C'CCGG_G
Cuts at: 1870 2679 7217 11096 13022 1870
Size: 809 4538 3879 1926 4545
Fragments arranged by size:
4545 4538 3879 1926 809
XmnI GAAnn'nnTTC
Cuts at: 11352 13826 11352
Size: 2474 13223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 13505 276
Size: 53 83 186 936 2668 9303 2468
Fragments arranged by size:
9303 2668 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I FseI FspAI NsiI
PacI PmlI RsrII SfiI SrfI SwaI