Restriction map for
pFN21AE2583
(Circular) (Six-base) MAPSORT of: pFN21AE2583.seq Check: 3243 from: 1 to: 9631
pFN21AE2583 9631 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4310 4310
Size: 9631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7441 278
Size: 53 83 186 936 5905 2468
Fragments arranged by size:
5905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3543 6974 1937
Size: 1606 3431 4594
Fragments arranged by size:
4594 3431 1606
Acc65I G'GTAC_C
Cuts at: 6952 6952
Size: 9631
AclI AA'CG_TT
Cuts at: 1563 7758 8131 1563
Size: 6195 373 3063
Fragments arranged by size:
6195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4388 4790 6189 6678 7694 9078 9346 1632
Size: 2756 402 1399 489 1016 1384 268 1917
Fragments arranged by size:
2756 1917 1399 1384 1016 489 402 268
AfeI AGC'GCT
Cuts at: 5920 5920
Size: 9631
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9434
AflIII A'CryG_T
Cuts at: 1203 8530 9507 1203
Size: 7327 977 1327
Fragments arranged by size:
7327 1327 977
AgeI A'CCGG_T
Cuts at: 8462 8462
Size: 9631
AhdI GACnn_n'nnGTC
Cuts at: 2986 8360 2986
Size: 5374 4257
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4328 1184
Size: 3144 6487
AlwNI CAG_nnn'CTG
Cuts at: 4315 4511 6923 7374 8946 4315
Size: 196 2412 451 1572 5000
Fragments arranged by size:
5000 2412 1572 451 196
ApaI G_GGCC'C
Cuts at: 2894 3309 2894
Size: 415 9216
ApaLI G'TGCA_C
Cuts at: 7688 8844 7688
Size: 1156 8475
ApoI r'AATT_y
Cuts at: 1493 2300 4415 4586 6940 7166 7318 9427 1493
Size: 807 2115 171 2354 226 152 2109 1697
Fragments arranged by size:
2354 2115 2109 1697 807 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 5199 8185 160
Size: 872 4167 2986 1606
Fragments arranged by size:
4167 2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9631
AvaI C'yCGr_G
Cuts at: 1870 1943 5862 6450 6956 1870
Size: 73 3919 588 506 4545
Fragments arranged by size:
4545 3919 588 506 73
AvrII C'CTAG_G
Cuts at: 6791 6791
Size: 9631
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3210 3243 4927 4960 3210
Size: 33 1684 33 7881
Fragments arranged by size:
7881 1684 33 33
BamHI G'GATC_C
Cuts at: 6961 7328 6961
Size: 367 9264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2765 2783 3334 5264 6952
Size: 325 217 627 978 18 551 1930 1688
Cuts at: 6952 8407 618
Size: 1455 1842
Fragments arranged by size:
1930 1842 1688 1455 978 627 551 325
217 18
BanII G_rGCy'C
Cuts at: 1391 2012 2027 2039 2894 3309 3726 6950 1391
Size: 621 15 12 855 415 417 3224 4072
Fragments arranged by size:
4072 3224 855 621 417 415 15 12
BbeI G_GCGC'C
Cuts at: 2787 5268 2787
Size: 2481 7150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 4345 6083 928
Size: 971 762 1684 1738 4476
Fragments arranged by size:
4476 1738 1684 971 762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7822 7856 1903
Size: 34 5885 34 3678
Fragments arranged by size:
5885 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2356 3960 5267 5971 7523 8733 2356
Size: 1604 1307 704 1552 1210 3254
Fragments arranged by size:
3254 1604 1552 1307 1210 704
BclI T'GATC_A
Cuts at: 1548 2493 5454 1548
Size: 945 2961 5725
Fragments arranged by size:
5725 2961 945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3313 4052 8242
Size: 107 122 71 631 605 1641 739 4190
Cuts at: 8242 136
Size: 1525
Fragments arranged by size:
4190 1641 1525 739 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3291 4578 4828 6827 9626 3291
Size: 1287 250 1999 2799 3296
Fragments arranged by size:
3296 2799 1999 1287 250
BlpI GC'TnA_GC
Cuts at: 3245 5775 6609 7048 3245
Size: 2530 834 439 5828
Fragments arranged by size:
5828 2530 834 439
Bme1580I G_kGCm'C
Cuts at: 1790 2865 2894 3309 6110 7692 8848 1790
Size: 1075 29 415 2801 1582 1156 2573
Fragments arranged by size:
2801 2573 1582 1156 1075 415 29
BmgBI CAC'GTC
Cuts at: 1318 2972 5994 6055 1318
Size: 1654 3022 61 4894
Fragments arranged by size:
4894 3022 1654 61
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2858 3499 3641
Size: 416 53 83 399 24 1441 641 142
Cuts at: 3641 6113 7362 8320 442
Size: 2472 1249 958 1753
Fragments arranged by size:
2472 1753 1441 1249 958 641 416 399
142 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4775 5119 4775
Size: 344 9287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 3219 3629 3752 3846 4258 4682 5262
Size: 1187 793 410 123 94 412 424 580
Cuts at: 5262 8291 1239
Size: 3029 2579
Fragments arranged by size:
3029 2579 1187 793 580 424 412 410
123 94
Bpu10I CC'TnA_GC
Cuts at: 5561 5561
Size: 9631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 4501 7756 8621 8919 2015
Size: 2486 3255 865 298 2727
Fragments arranged by size:
3255 2727 2486 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 8294 882
Size: 617 1380 5415 2219
Fragments arranged by size:
5415 2219 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 2563 4987 7422 493
Size: 711 1359 2424 2435 2702
Fragments arranged by size:
2702 2435 2424 1359 711
BsaBI GATnn'nnATC
Cuts at: 4712 5009 6757 9625 4712
Size: 297 1748 2868 4718
Fragments arranged by size:
4718 2868 1748 297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2784 5265 7438 7820
Size: 53 83 186 936 1251 2481 2173 382
Cuts at: 7820 8455 275
Size: 635 1451
Fragments arranged by size:
2481 2173 1451 1251 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 8064 8462 8736 8883 9294 8064
Size: 398 274 147 411 8401
Fragments arranged by size:
8401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2573 2603 4073 4103 1178
Size: 30 165 30 1170 30 1470 30 6706
Fragments arranged by size:
6706 1470 1170 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2578 3626 3762 4547 5108 5246 5585 5612
Size: 1392 1048 136 785 561 138 339 27
Cuts at: 5612 1186
Size: 5205
Fragments arranged by size:
5205 1392 1048 785 561 339 138 136
27
BseYI C'CCAG_C
Cuts at: 2195 8834 9330 2195
Size: 6639 496 2496
Fragments arranged by size:
6639 2496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5630 1163
Size: 573 3894 5164
Fragments arranged by size:
5164 3894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5500 7081 7842 8870 1540
Size: 275 186 3499 1581 761 1028 2301
Fragments arranged by size:
3499 2301 1581 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2039 2727 4545 5052 5619 7692 7777 8848
Size: 27 688 1818 507 567 2073 85 1071
Cuts at: 8848 2012
Size: 2795
Fragments arranged by size:
2795 2073 1818 1071 688 567 507 85
27
BsmI GAATG_Cn'
Cuts at: 3502 5812 6932 7142 7235 3502
Size: 2310 1120 210 93 5898
Fragments arranged by size:
5898 2310 1120 210 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5495 5495
Size: 9631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2027 2039 2727 2865 2894 3309
Size: 399 222 15 12 688 138 29 415
Cuts at: 3309 3726 4545 5052 5619 6110 6950 7692 7777
Size: 417 819 507 567 491 840 742 85
Cuts at: 7777 8848 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 840 819 742 688 567 507
491 417 415 399 222 138 85 29
15 12
BspEI T'CCGG_A
Cuts at: 9294 9294
Size: 9631
BspHI T'CATG_A
Cuts at: 4851 5052 5425 7520 9250 4851
Size: 201 373 2095 1730 5232
Fragments arranged by size:
5232 2095 1730 373 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2931 4259 4310 4653 5337 6986 844
Size: 2087 1328 51 343 684 1649 3489
Fragments arranged by size:
3489 2087 1649 1328 684 343 51
BsrBI CCG'CTC
Cuts at: 1394 1823 7518 1394
Size: 429 5695 3507
Fragments arranged by size:
5695 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4290 6539 8126 8300 65
Size: 1183 3042 2249 1587 174 1396
Fragments arranged by size:
3042 2249 1587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8275 8462 1700
Size: 110 6465 187 2869
Fragments arranged by size:
6465 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9631
BssSI C'ACGA_G
Cuts at: 2867 2876 3725 3959 4082 4832 7691 8703 2867
Size: 9 849 234 123 750 2859 1012 3795
Fragments arranged by size:
3795 2859 1012 849 750 234 123 9
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2860 3514 5608 794
Size: 2066 654 2094 4817
Fragments arranged by size:
4817 2094 2066 654
BstBI TT'CG_AA
Cuts at: 8435 8435
Size: 9631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9631
BstXI CCAn_nnnn'nTGG
Cuts at: 2914 2947 4048 2914
Size: 33 1101 8497
Fragments arranged by size:
8497 1101 33
BstYI r'GATC_y
Cuts at: 1972 2523 3291 4578 4755 4828 6827 6961 7328
Size: 551 768 1287 177 73 1999 134 367
Cuts at: 7328 7713 7730 9182 9268 9280 9626 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1999 1977 1452 1287 768 551 385 367
346 177 134 86 73 17 12
Bsu36I CC'TnA_GG
Cuts at: 3038 3038
Size: 9631
BtgI C'CryG_G
Cuts at: 513 1065 2630 3381 4074 4266 5190 513
Size: 552 1565 751 693 192 924 4954
Fragments arranged by size:
4954 1565 924 751 693 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3527 487
Size: 683 56 2301 6591
Fragments arranged by size:
6591 2301 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 3984 4011 5609 6606 7147 7941 7961 2369
Size: 1615 27 1598 997 541 794 20 4039
Fragments arranged by size:
4039 1615 1598 997 794 541 27 20
ClaI AT'CG_AT
Cuts at: 1554 5941 1554
Size: 4387 5244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9596
DraI TTT'AAA
Cuts at: 6264 6935 6264
Size: 671 8960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5435 8638 809
Size: 507 4119 3203 1802
Fragments arranged by size:
4119 3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 3314 3384
Size: 54 1425 50 275 18 319 1165 70
Cuts at: 3384 4038 4150 4263 4688 4836 4900 5497 7078
Size: 654 112 113 425 148 64 597 1581
Cuts at: 7078 7967 9596 8
Size: 889 1629 43
Fragments arranged by size:
1629 1581 1425 1165 889 654 597 425
319 275 148 113 112 70 64 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5497 7078 1537
Size: 275 3685 1581 4090
Fragments arranged by size:
4090 3685 1581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2925 3397 3897 4023 4476 5561 5955
Size: 849 723 472 500 126 453 1085 394
Cuts at: 5955 6039 6165 7561 1353
Size: 84 126 1396 3423
Fragments arranged by size:
3423 1396 1085 849 723 500 472 453
394 126 126 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2328 3033 3678 8218 8592 8738
Size: 1064 138 739 705 645 4540 374 146
Cuts at: 8738 387
Size: 1280
Fragments arranged by size:
4540 1280 1064 739 705 645 374 146
138
EcoICRI GAG'CTC
Cuts at: 2010 2037 2010
Size: 27 9604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 3219 3629 3752 3846 4258 4388
Size: 393 794 793 410 123 94 412 130
Cuts at: 4388 4682 4790 5262 6189 6678 7694 8291 9078
Size: 294 108 472 927 489 1016 597 787
Cuts at: 9078 9346 1239
Size: 268 1524
Fragments arranged by size:
1524 1016 927 794 793 787 597 489
472 412 410 393 294 268 130 123
108 94
EcoNI CCTnn'n_nnAGG
Cuts at: 2495 5849 2495
Size: 3354 6277
EcoO109I rG'GnC_Cy
Cuts at: 2517 2890 2891 3306 4045 2517
Size: 373 1 415 739 8103
Fragments arranged by size:
8103 739 415 373 1
EcoRI G'AATT_C
Cuts at: 2300 4586 6940 7318 2300
Size: 2286 2354 378 4613
Fragments arranged by size:
4613 2354 2286 378
EcoRV GAT'ATC
Cuts at: 4924 5405 5945 4924
Size: 481 540 8610
Fragments arranged by size:
8610 540 481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4820 5748 4820
Size: 928 8703
FspI TGC'GCA
Cuts at: 2997 8137 2997
Size: 5140 4491
HaeII r_GCGC'y
Cuts at: 1678 2787 5268 5922 8778 1678
Size: 1109 2481 654 2856 2531
Fragments arranged by size:
2856 2531 2481 1109 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2573 2605 2972
Size: 32 135 32 659 32 310 32 367
Cuts at: 2972 3004 4100 4132 4421 4453 4499 4531 5538
Size: 32 1096 32 289 32 46 32 1007
Cuts at: 5538 5570 5630 5662 5966 5998 8272 8304 8346
Size: 32 60 32 304 32 2274 32 42
Cuts at: 8346 8378 1373
Size: 32 2626
Fragments arranged by size:
2626 2274 1096 1007 659 367 310 304
289 135 60 46 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3544 4966 5761 6478 6975 7221
Size: 988 281 1606 1422 795 717 497 246
Cuts at: 7221 669
Size: 3079
Fragments arranged by size:
3079 1606 1422 988 795 717 497 281
246
HindIII A'AGCT_T
Cuts at: 748 6436 6909 748
Size: 5688 473 3470
Fragments arranged by size:
5688 3470 473
HpaI GTT'AAC
Cuts at: 6478 7221 6478
Size: 743 8888
KasI G'GCGC_C
Cuts at: 2783 5264 2783
Size: 2481 7150
KpnI G_GTAC'C
Cuts at: 6956 6956
Size: 9631
MfeI C'AATT_G
Cuts at: 6515 7230 6515
Size: 715 8916
MluI A'CGCG_T
Cuts at: 9507 9507
Size: 9631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2644 2724 5403 5688 6570 8745 8929 1305
Size: 1339 80 2679 285 882 2175 184 2007
Fragments arranged by size:
2679 2175 2007 1339 882 285 184 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2151 3386 4040 4838 10
Size: 54 1425 343 319 1235 654 798 4803
Fragments arranged by size:
4803 1425 1235 798 654 343 319 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3188 3917 4670 4850 5483 7589
Size: 648 1779 243 729 753 180 633 2106
Cuts at: 7589 7948 8107 518
Size: 359 159 2042
Fragments arranged by size:
2106 2042 1779 753 729 648 633 359
243 180 159
MspA1I CmG'CkG
Cuts at: 1606 2401 2967 3103 3244 3759 5410 5496 5502
Size: 795 566 136 141 515 1651 86 6
Cuts at: 5502 7047 7724 8872 9117 1606
Size: 1545 677 1148 245 2120
Fragments arranged by size:
2120 1651 1545 1148 795 677 566 515
245 141 136 86 6
NarI GG'CG_CC
Cuts at: 2784 5265 2784
Size: 2481 7150
NcoI C'CATG_G
Cuts at: 513 1065 2630 3381 4074 5190 513
Size: 552 1565 751 693 1116 4954
Fragments arranged by size:
4954 1565 1116 751 693 552
NdeI CA'TA_TG
Cuts at: 387 4426 387
Size: 4039 5592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9631
NotI GC'GGCC_GC
Cuts at: 5497 7078 5497
Size: 1581 8050
NspI r_CATG'y
Cuts at: 1132 1229 3583 5020 5137 6387 6839 6989 8534
Size: 97 2354 1437 117 1250 452 150 1545
Cuts at: 8534 1132
Size: 2229
Fragments arranged by size:
2354 2229 1545 1437 1250 452 150 117
97
PasI CC'CwG_GG
Cuts at: 1348 2770 3322 1348
Size: 1422 552 7657
Fragments arranged by size:
7657 1422 552
PciI A'CATG_T
Cuts at: 8530 8530
Size: 9631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 4000 4126 6194 1108
Size: 1275 1617 126 2068 4545
Fragments arranged by size:
4545 2068 1617 1275 126
PfoI T'CCnGG_A
Cuts at: 4698 4947 5622 4698
Size: 249 675 8707
Fragments arranged by size:
8707 675 249
PmeI GTTT'AAAC
Cuts at: 6935 6935
Size: 9631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7700 7732 9213 9245 7700
Size: 32 1481 32 8086
Fragments arranged by size:
8086 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 6008 1613
Size: 4395 5236
PsiI TTA'TAA
Cuts at: 3081 3116 3621 7201 3081
Size: 35 505 3580 5511
Fragments arranged by size:
5511 3580 505 35
PspOMI G'GGCC_C
Cuts at: 2890 3305 2890
Size: 415 9216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4871 4903 4871
Size: 32 9599
PstI C_TGCA'G
Cuts at: 830 2832 4307 5507 5754 6601 6983 830
Size: 2002 1475 1200 247 847 382 3478
Fragments arranged by size:
3478 2002 1475 1200 847 382 247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9631
PvuII CAG'CTG
Cuts at: 2401 3103 3244 5410 2401
Size: 702 141 2166 6622
Fragments arranged by size:
6622 2166 702 141
SacI G_AGCT'C
Cuts at: 2012 2039 2012
Size: 27 9604
SalI G'TCGA_C
Cuts at: 1936 3542 6973 1936
Size: 1606 3431 4594
Fragments arranged by size:
4594 3431 1606
SapI GCTCTTCn'nnn_
Cuts at: 2925 5561 2925
Size: 2636 6995
SbfI CC_TGCA'GG
Cuts at: 6983 6983
Size: 9631
ScaI AGT'ACT
Cuts at: 3005 4814 7879 9591 3005
Size: 1809 3065 1712 3045
Fragments arranged by size:
3065 3045 1809 1712
SexAI A'CCwGG_T
Cuts at: 2658 5382 2658
Size: 2724 6907
SfcI C'TryA_G
Cuts at: 826 1045 2616 2828 4303 4874 5503 5750 5929
Size: 219 1571 212 1475 571 629 247 179
Cuts at: 5929 6597 6979 8114 8795 8986 826
Size: 668 382 1135 681 191 1471
Fragments arranged by size:
1571 1475 1471 1135 681 668 629 571
382 247 219 212 191 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 3313 4052 3313
Size: 739 8892
SfoI GGC'GCC
Cuts at: 2785 5266 2785
Size: 2481 7150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9631
SmaI CCC'GGG
Cuts at: 1872 5864 6452 6958 1872
Size: 3992 588 506 4545
Fragments arranged by size:
4545 3992 588 506
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 4516 7735 8636 8898 820
Size: 197 926 87 2486 3219 901 262 1553
Fragments arranged by size:
3219 2486 1553 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 7422 493
Size: 6929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9631
SphI G_CATG'C
Cuts at: 1132 6989 1132
Size: 5857 3774
SspI AAT'ATT
Cuts at: 5 52 7555 5
Size: 47 7503 2081
Fragments arranged by size:
7503 2081 47
StuI AGG'CCT
Cuts at: 1517 2439 2490 3820 1517
Size: 922 51 1330 7328
Fragments arranged by size:
7328 1330 922 51
StyI C'CwwG_G
Cuts at: 513 1065 2630 3381 3393 4041 4074 4452 5190
Size: 552 1565 751 12 648 33 378 738
Cuts at: 5190 6791 7070 513
Size: 1601 279 3074
Fragments arranged by size:
3074 1601 1565 751 738 648 552 378
279 33 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 2279 2756 3394 3481 4337 7658
Size: 1255 629 70 477 638 87 856 3321
Cuts at: 7658 7675 7828 8013 8458 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3321 1498 1255 856 638 629 477 445
185 153 87 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3003 4812 5022
Size: 274 80 33 51 1443 1026 1809 210
Cuts at: 5022 6492 7877 9589 96
Size: 1470 1385 1712 138
Fragments arranged by size:
1809 1712 1470 1443 1385 1026 274 210
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4073 4105 6559 6591 9591 9623 4073
Size: 32 2454 32 3000 32 4081
Fragments arranged by size:
4081 3000 2454 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2695 3633 1316
Size: 1379 938 7314
Fragments arranged by size:
7314 1379 938
XbaI T'CTAG_A
Cuts at: 6967 6967
Size: 9631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3604 4048 4408 5317 3604
Size: 444 360 909 7918
Fragments arranged by size:
7918 909 444 360
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9631
XmaI C'CCGG_G
Cuts at: 1870 5862 6450 6956 1870
Size: 3992 588 506 4545
Fragments arranged by size:
4545 3992 588 506
XmnI GAAnn'nnTTC
Cuts at: 3163 5555 6663 7760 3163
Size: 2392 1108 1097 5034
Fragments arranged by size:
5034 2392 1108 1097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7439 276
Size: 53 83 186 936 5905 2468
Fragments arranged by size:
5905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbvCI BfrBI BsiWI BstZ17I DraIII FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI RsrII SacII SanDI SrfI SwaI