Restriction map for
pFN21AE2582
(Circular) (Six-base) MAPSORT of: pFN21AE2582.seq Check: 6590 from: 1 to: 7573
pFN21AE2582 7573 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2460 3470 4916 1937
Size: 523 1010 1446 4594
Fragments arranged by size:
4594 1446 1010 523
Acc65I G'GTAC_C
Cuts at: 3093 4894 3093
Size: 1801 5772
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2590 2774 2783 3365 3416 5636 7020 7288
Size: 958 184 9 582 51 2220 1384 268
Cuts at: 7288 1632
Size: 1917
Fragments arranged by size:
2220 1917 1384 958 582 268 184 51
9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 6472 7449 1203
Size: 5269 977 1327
Fragments arranged by size:
5269 1327 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 6302 6302
Size: 7573
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4511 4545 4841 1184
Size: 3327 34 296 3916
Fragments arranged by size:
3916 3327 296 34
AlwNI CAG_nnn'CTG
Cuts at: 2308 3850 4124 4133 4406 5316 6888 2308
Size: 1542 274 9 273 910 1572 2993
Fragments arranged by size:
2993 1572 1542 910 274 273 9
ApaI G_GGCC'C
Cuts at: 2886 2886
Size: 7573
ApaLI G'TGCA_C
Cuts at: 5630 6786 5630
Size: 1156 6417
ApoI r'AATT_y
Cuts at: 1493 3438 3659 4882 5108 5260 7369 1493
Size: 1945 221 1223 226 152 2109 1697
Fragments arranged by size:
2109 1945 1697 1223 226 221 152
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2848 2922 4835 4898 1870
Size: 73 905 74 1913 63 4545
Fragments arranged by size:
4545 1913 905 74 73 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2924 2957 2924
Size: 33 7540
BamHI G'GATC_C
Cuts at: 4032 4903 5270 4032
Size: 871 367 6335
Fragments arranged by size:
6335 871 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2672 3093 4894 6349
Size: 325 217 627 546 339 421 1801 1455
Cuts at: 6349 618
Size: 1842
Fragments arranged by size:
1842 1801 1455 627 546 421 339 325
217
BanII G_rGCy'C
Cuts at: 1391 2886 3328 4190 4659 4892 1391
Size: 1495 442 862 469 233 4072
Fragments arranged by size:
4072 1495 862 469 442 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 2451 3538 3624 4355 4478 928
Size: 971 210 342 1087 86 731 123 4023
Fragments arranged by size:
4023 1087 971 731 342 210 123 86
BbvCI CC'TCA_GC
Cuts at: 2547 3060 4452 4558 2547
Size: 513 1392 106 5562
Fragments arranged by size:
5562 1392 513 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4313 4347 5764 5798 1903
Size: 34 2376 34 1417 34 3678
Fragments arranged by size:
3678 2376 1417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5465 6675 5465
Size: 1210 6363
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4046 6184 136
Size: 107 122 71 631 605 2374 2138 1525
Fragments arranged by size:
2374 2138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7568 7568
Size: 7573
BlpI GC'TnA_GC
Cuts at: 2786 4700 4990 2786
Size: 1914 290 5369
Fragments arranged by size:
5369 1914 290
Bme1580I G_kGCm'C
Cuts at: 1790 2675 2886 4603 5634 6790 1790
Size: 885 211 1717 1031 1156 2573
Fragments arranged by size:
2573 1717 1156 1031 885 211
BmgBI CAC'GTC
Cuts at: 1318 2776 1318
Size: 1458 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2073 2966 4416
Size: 416 53 83 399 24 656 893 1450
Cuts at: 4416 5304 6262 442
Size: 888 958 1753
Fragments arranged by size:
1753 1450 958 893 888 656 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4543 4543
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2834 3935 4064 4100 4214 4345 4787 6233
Size: 1595 1101 129 36 114 131 442 1446
Cuts at: 6233 1239
Size: 2579
Fragments arranged by size:
2579 1595 1446 1101 442 131 129 114
36
Bpu10I CC'TnA_GC
Cuts at: 2547 3060 4452 4558 2547
Size: 513 1392 106 5562
Fragments arranged by size:
5562 1392 513 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2424 5698 6563 6861 2424
Size: 3274 865 298 3136
Fragments arranged by size:
3274 3136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3546 6236 882
Size: 617 2047 2690 2219
Fragments arranged by size:
2690 2219 2047 617
BsaAI yAC'GTr
Cuts at: 493 1204 5364 493
Size: 711 4160 2702
Fragments arranged by size:
4160 2702 711
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5380 5762 6397 275
Size: 53 83 186 936 3847 382 635 1451
Fragments arranged by size:
3847 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2532 6006 6404 6678 6825 7236 2532
Size: 3474 398 274 147 411 2869
Fragments arranged by size:
3474 2869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 3203 3233 4058
Size: 30 165 30 810 30 960 30 825
Cuts at: 4058 4088 1178
Size: 30 4663
Fragments arranged by size:
4663 960 825 810 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 3048 3368 3809 4040 4097 4229 4232
Size: 1408 454 320 441 231 57 132 3
Cuts at: 4232 4235 4348 1186
Size: 3 113 4411
Fragments arranged by size:
4411 1408 454 441 320 231 132 113
57 3 3
BseYI C'CCAG_C
Cuts at: 2895 3281 3327 3855 3942 3994 4501 4667 4685
Size: 386 46 528 87 52 507 166 18
Cuts at: 4685 4738 6776 7272 2895
Size: 53 2038 496 3196
Fragments arranged by size:
3196 2038 528 507 496 386 166 87
53 52 46 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2409 3194 4250 4811 1163
Size: 573 673 785 1056 561 3925
Fragments arranged by size:
3925 1056 785 673 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 5023 5784 6812 1540
Size: 275 186 2695 327 761 1028 2301
Fragments arranged by size:
2695 2301 1028 761 327 275 186
BsiHKAI G_wGCw'C
Cuts at: 2994 3256 4091 4190 4515 4659 4706 5634 5719
Size: 262 835 99 325 144 47 928 85
Cuts at: 5719 6790 2994
Size: 1071 3777
Fragments arranged by size:
3777 1071 928 835 325 262 144 99
85 47
BsmI GAATG_Cn'
Cuts at: 4117 5084 5177 4117
Size: 967 93 6513
Fragments arranged by size:
6513 967 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2675 2886 2994 3256 3328 4091 4190
Size: 399 885 211 108 262 72 763 99
Cuts at: 4190 4515 4603 4659 4706 4892 5634 5719 6790
Size: 325 88 56 47 186 742 85 1071
Cuts at: 6790 1391
Size: 2174
Fragments arranged by size:
2174 1071 885 763 742 399 325 262
211 186 108 99 88 85 72 56
47
BspEI T'CCGG_A
Cuts at: 7236 7236
Size: 7573
BspHI T'CATG_A
Cuts at: 3538 5462 7192 3538
Size: 1924 1730 3919
Fragments arranged by size:
3919 1924 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 4928 844
Size: 2050 2034 3489
Fragments arranged by size:
3489 2050 2034
BsrBI CCG'CTC
Cuts at: 1394 1823 2781 3895 4030 4380 5460 1394
Size: 429 958 1114 135 350 1080 3507
Fragments arranged by size:
3507 1114 1080 958 429 350 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 6068 6242 65
Size: 1183 4820 174 1396
Fragments arranged by size:
4820 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4695 6217 6404 1700
Size: 110 2885 1522 187 2869
Fragments arranged by size:
2885 2869 1522 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 3032 5633 6645 3032
Size: 2601 1012 3960
Fragments arranged by size:
3960 2601 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4124 794
Size: 3330 4243
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 3008 1184
Size: 1824 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 3020 3293 3020
Size: 273 7300
BstYI r'GATC_y
Cuts at: 1972 2209 2247 3741 4032 4903 5270 5655 5672
Size: 237 38 1494 291 871 367 385 17
Cuts at: 5672 7124 7210 7222 7568 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1494 1452 871 385 367 346 291
237 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2243
Size: 7573
BtgI C'CryG_G
Cuts at: 513 1065 2424 2650 4690 4783 513
Size: 552 1359 226 2040 93 3303
Fragments arranged by size:
3303 2040 1359 552 226 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6834
Fragments arranged by size:
6834 683 56
BtsI GCAGTG_nn'
Cuts at: 3206 3708 4122 4535 4603 5089 5883 5903 3206
Size: 502 414 413 68 486 794 20 4876
Fragments arranged by size:
4876 794 502 486 414 413 68 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 4877 4877
Size: 7573
DraIII CAC_nnn'GTG
Cuts at: 2503 2503
Size: 7573
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 3081 3961
Size: 54 1425 50 275 18 531 720 880
Cuts at: 3961 4693 4743 4780 5020 5909 7538 8
Size: 732 50 37 240 889 1629 43
Fragments arranged by size:
1629 1425 889 880 732 720 531 275
240 54 50 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 5020 1537
Size: 275 2881 327 4090
Fragments arranged by size:
4090 2881 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 3786 4461 4664 5503 1353
Size: 1843 590 675 203 839 3423
Fragments arranged by size:
3423 1843 839 675 590 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3184 6160 6534 6680 387
Size: 1064 138 1595 2976 374 146 1280
Fragments arranged by size:
2976 1595 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4188 4657 4188
Size: 469 7104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2590 2774 2783 2834 3365 3416 3935
Size: 393 958 184 9 51 531 51 519
Cuts at: 3935 4064 4100 4214 4345 4787 5636 6233 7020
Size: 129 36 114 131 442 849 597 787
Cuts at: 7020 7288 1239
Size: 268 1524
Fragments arranged by size:
1524 958 849 787 597 531 519 442
393 268 184 131 129 114 51 51
36 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3749 3749
Size: 7573
EcoO109I rG'GnC_Cy
Cuts at: 2055 2882 2883 2927 3847 4315 2055
Size: 827 1 44 920 468 5313
Fragments arranged by size:
5313 920 827 468 44 1
EcoRI G'AATT_C
Cuts at: 3659 4882 5260 3659
Size: 1223 378 5972
Fragments arranged by size:
5972 1223 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2231 2231
Size: 7573
FspI TGC'GCA
Cuts at: 6079 6079
Size: 7573
HaeII r_GCGC'y
Cuts at: 1678 2753 6720 1678
Size: 1075 3967 2531
Fragments arranged by size:
3967 2531 1075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4699 4731 4797
Size: 32 135 32 2830 32 265 32 66
Cuts at: 4797 4829 6214 6246 6288 6320 1373
Size: 32 1385 32 42 32 2626
Fragments arranged by size:
2830 2626 1385 265 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4917 5163 669
Size: 988 281 2979 246 3079
Fragments arranged by size:
3079 2979 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KpnI G_GTAC'C
Cuts at: 3097 4898 3097
Size: 1801 5772
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4120 6687 6871 1305
Size: 2815 2567 184 2007
Fragments arranged by size:
2815 2567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 3963 4782 10
Size: 54 1425 343 531 1600 819 2801
Fragments arranged by size:
2801 1600 1425 819 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3957 4521 5531 5890 6049 518
Size: 648 2791 564 1010 359 159 2042
Fragments arranged by size:
2791 2042 1010 648 564 359 159
MspA1I CmG'CkG
Cuts at: 1606 2288 2305 2806 3201 3859 4133 4447 4692
Size: 682 17 501 395 658 274 314 245
Cuts at: 4692 4742 4989 5666 6814 7059 1606
Size: 50 247 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 658 501 395 314
274 247 245 245 50 17
NaeI GCC'GGC
Cuts at: 4697 4697
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 2650 4783 513
Size: 552 1585 2133 3303
Fragments arranged by size:
3303 2133 1585 552
NdeI CA'TA_TG
Cuts at: 387 3817 387
Size: 3430 4143
NgoMIV G'CCGG_C
Cuts at: 4695 4695
Size: 7573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 3873 3979 4526 4931 6476 1132
Size: 97 2644 106 547 405 1545 2229
Fragments arranged by size:
2644 2229 1545 547 405 106 97
PasI CC'CwG_GG
Cuts at: 1348 3020 3247 1348
Size: 1672 227 5674
Fragments arranged by size:
5674 1672 227
PciI A'CATG_T
Cuts at: 6472 6472
Size: 7573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3037 3334 1108
Size: 1929 297 5347
Fragments arranged by size:
5347 1929 297
PfoI T'CCnGG_A
Cuts at: 2313 3744 4002 2313
Size: 1431 258 5884
Fragments arranged by size:
5884 1431 258
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2927 4315 2055
Size: 872 1388 5313
Fragments arranged by size:
5313 1388 872
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 2882 2882
Size: 7573
PstI C_TGCA'G
Cuts at: 830 2905 3631 3843 3864 4129 4323 4925 830
Size: 2075 726 212 21 265 194 602 3478
Fragments arranged by size:
3478 2075 726 602 265 212 194 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 2288 2806 3859 4133 4447 2288
Size: 518 1053 274 314 5414
Fragments arranged by size:
5414 1053 518 314 274
SacI G_AGCT'C
Cuts at: 4190 4659 4190
Size: 469 7104
SacII CC_GC'GG
Cuts at: 4693 4693
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SanDI GG'GwC_CC
Cuts at: 2055 2055
Size: 7573
SapI GCTCTTCn'nnn_
Cuts at: 4664 4664
Size: 7573
SbfI CC_TGCA'GG
Cuts at: 2905 4925 2905
Size: 2020 5553
ScaI AGT'ACT
Cuts at: 5821 7533 5821
Size: 1712 5861
SexAI A'CCwGG_T
Cuts at: 2499 2499
Size: 7573
SfcI C'TryA_G
Cuts at: 826 1045 2901 3627 3839 3860 4125 4319 4921
Size: 219 1856 726 212 21 265 194 602
Cuts at: 4921 6056 6737 6928 826
Size: 1135 681 191 1471
Fragments arranged by size:
1856 1471 1135 726 681 602 265 219
212 194 191 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 4046 4046
Size: 7573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 4900 1872
Size: 3028 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2439 5677 6578 6840 820
Size: 197 926 496 3238 901 262 1553
Fragments arranged by size:
3238 1553 926 901 496 262 197
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 4526 4931 1132
Size: 3394 405 3774
Fragments arranged by size:
3774 3394 405
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7573
StyI C'CwwG_G
Cuts at: 513 1065 2650 4783 5012 513
Size: 552 1585 2133 229 3074
Fragments arranged by size:
3074 2133 1585 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3239 4567 4752 5600 5617 5770 5955
Size: 1255 1659 1328 185 848 17 153 185
Cuts at: 5955 6400 325
Size: 445 1498
Fragments arranged by size:
1659 1498 1328 1255 848 445 185 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5819 7531 96
Size: 274 80 33 51 1443 3842 1712 138
Fragments arranged by size:
3842 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3700 3732 7533 7565 3203
Size: 32 465 32 3801 32 3211
Fragments arranged by size:
3801 3211 465 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 2098 4909 2098
Size: 2811 4762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3950 4166 4444 3950
Size: 216 278 7079
Fragments arranged by size:
7079 278 216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 4898 1870
Size: 3028 4545
XmnI GAAnn'nnTTC
Cuts at: 5702 5702
Size: 7573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SfoI SrfI
SwaI