Restriction map for
pFN21AE2581
(Circular) (Six-base) MAPSORT of: pFN21AE2581.seq Check: 6166 from: 1 to: 7762
pFN21AE2581 7762 bp
With 182 enzymes: SgfI *
July 22, 2015 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 5572 278
Size: 53 83 186 936 3298 738 2468
Fragments arranged by size:
3298 2468 936 738 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4440 5105 1937
Size: 2503 665 4594
Fragments arranged by size:
4594 2503 665
Acc65I G'GTAC_C
Cuts at: 5083 5083
Size: 7762
AclI AA'CG_TT
Cuts at: 1563 5889 6262 1563
Size: 4326 373 3063
Fragments arranged by size:
4326 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 2577 3001 5825 7209 7477 1632
Size: 550 395 424 2824 1384 268 1917
Fragments arranged by size:
2824 1917 1384 550 424 395 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7565
AflIII A'CryG_T
Cuts at: 1203 4435 6661 7638 1203
Size: 3232 2226 977 1327
Fragments arranged by size:
3232 2226 1327 977
AgeI A'CCGG_T
Cuts at: 6593 6593
Size: 7762
AhdI GACnn_n'nnGTC
Cuts at: 6491 6491
Size: 7762
AleI CACnn'nnGTG
Cuts at: 1166 2328 2660 3353 1166
Size: 1162 332 693 5575
Fragments arranged by size:
5575 1162 693 332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7762
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 7730
AlwNI CAG_nnn'CTG
Cuts at: 2977 3538 3553 3841 5505 7077 2977
Size: 561 15 288 1664 1572 3662
Fragments arranged by size:
3662 1664 1572 561 288 15
ApaLI G'TGCA_C
Cuts at: 5819 6975 5819
Size: 1156 6606
ApoI r'AATT_y
Cuts at: 1493 3284 4783 4823 5071 5297 5449 7558 1493
Size: 1791 1499 40 248 226 152 2109 1697
Fragments arranged by size:
2109 1791 1697 1499 248 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 3810 6316 160
Size: 872 2778 2506 1606
Fragments arranged by size:
2778 2506 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 5087 1870
Size: 73 194 2950 4545
Fragments arranged by size:
4545 2950 194 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4633 4666 4633
Size: 33 7729
BamHI G'GATC_C
Cuts at: 3961 5092 5459 3961
Size: 1131 367 6264
Fragments arranged by size:
6264 1131 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 3738 4932 5083 6538
Size: 325 217 627 1538 413 1194 151 1455
Cuts at: 6538 618
Size: 1842
Fragments arranged by size:
1842 1538 1455 1194 627 413 325 217
151
BanII G_rGCy'C
Cuts at: 1391 2151 3954 4053 5081 1391
Size: 760 1803 99 1028 4072
Fragments arranged by size:
4072 1803 1028 760 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4845 4938 928
Size: 971 2946 93 3752
Fragments arranged by size:
3752 2946 971 93
BbvCI CC'TCA_GC
Cuts at: 2355 3531 2355
Size: 1176 6586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5953 5987 1903
Size: 34 50 34 3932 34 3678
Fragments arranged by size:
3932 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5654 6864 5654
Size: 1210 6552
BclI T'GATC_A
Cuts at: 1548 3915 4968 1548
Size: 2367 1053 4342
Fragments arranged by size:
4342 2367 1053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 2719 2832
Size: 107 122 71 631 605 821 226 113
Cuts at: 2832 3797 4269 6373 136
Size: 965 472 2104 1525
Fragments arranged by size:
2104 1525 965 821 631 605 472 226
122 113 107 71
BglII A'GATC_T
Cuts at: 7757 7757
Size: 7762
BlpI GC'TnA_GC
Cuts at: 5179 5179
Size: 7762
Bme1580I G_kGCm'C
Cuts at: 1790 2565 2667 4937 5823 6979 1790
Size: 775 102 2270 886 1156 2573
Fragments arranged by size:
2573 2270 1156 886 775 102
BmgBI CAC'GTC
Cuts at: 1318 4438 1318
Size: 3120 4642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3347 4172 4936
Size: 416 53 83 399 24 1930 825 764
Cuts at: 4936 5493 6451 442
Size: 557 958 1753
Fragments arranged by size:
1930 1753 958 825 764 557 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2331 4306 4613 4763 6422 1239
Size: 966 126 1975 307 150 1659 2579
Fragments arranged by size:
2579 1975 1659 966 307 150 126
Bpu10I CC'TnA_GC
Cuts at: 2355 3531 3948 4047 2355
Size: 1176 417 99 6070
Fragments arranged by size:
6070 1176 417 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 4397 5887 6752 7050 4138
Size: 259 1490 865 298 4850
Fragments arranged by size:
4850 1490 865 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6425 882
Size: 617 4926 2219
Fragments arranged by size:
4926 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 5553 493
Size: 711 1458 2891 2702
Fragments arranged by size:
2891 2702 1458 711
BsaBI GATnn'nnATC
Cuts at: 7756 7756
Size: 7762
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4340 4599 4831 5569
Size: 53 83 186 936 2807 259 232 738
Cuts at: 5569 5951 6586 275
Size: 382 635 1451
Fragments arranged by size:
2807 1451 936 738 635 382 259 232
186 83 53
BsaWI w'CCGG_w
Cuts at: 2599 2707 6195 6593 6867 7014 7425 2599
Size: 108 3488 398 274 147 411 2936
Fragments arranged by size:
3488 2936 411 398 274 147 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2407 2437 3305 3335 3506
Size: 30 165 30 1004 30 868 30 171
Cuts at: 3506 3536 1178
Size: 30 5404
Fragments arranged by size:
5404 1004 868 171 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3327 3386 3512 3620 4586 4883 1186
Size: 2141 59 126 108 966 297 4065
Fragments arranged by size:
4065 2141 966 297 126 108 59
BseYI C'CCAG_C
Cuts at: 2683 3264 3475 3546 4272 6965 7461 2683
Size: 581 211 71 726 2693 496 2984
Fragments arranged by size:
2984 2693 726 581 496 211 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3134 3363 1163
Size: 573 1398 229 5562
Fragments arranged by size:
5562 1398 573 229
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 2827 5212 5973 7001 1540
Size: 275 186 814 12 2385 761 1028 2301
Fragments arranged by size:
2385 2301 1028 814 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 4045 4296 5823 5908 6979 4045
Size: 251 1527 85 1071 4828
Fragments arranged by size:
4828 1527 1071 251 85
BsiWI C'GTAC_G
Cuts at: 2897 2897
Size: 7762
BsmI GAATG_Cn'
Cuts at: 5273 5366 5273
Size: 93 7669
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2565 2667 3954 4045 4053 4296
Size: 399 361 414 102 1287 91 8 243
Cuts at: 4296 4937 5081 5823 5908 6979 1391
Size: 641 144 742 85 1071 2174
Fragments arranged by size:
2174 1287 1071 742 641 414 399 361
243 144 102 91 85 8
BspEI T'CCGG_A
Cuts at: 7425 7425
Size: 7762
BspHI T'CATG_A
Cuts at: 5651 7381 5651
Size: 1730 6032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 5117 844
Size: 1782 2491 3489
Fragments arranged by size:
3489 2491 1782
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 4474 5649 1394
Size: 429 2245 406 1175 3507
Fragments arranged by size:
3507 2245 1175 429 406
BsrDI GCAATG_nn'
Cuts at: 65 1248 2171 4376 6257 6431 65
Size: 1183 923 2205 1881 174 1396
Fragments arranged by size:
2205 1881 1396 1183 923 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3735 4994 6406 6593 1700
Size: 110 1925 1259 1412 187 2869
Fragments arranged by size:
2869 1925 1412 1259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7762
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7762
BssSI C'ACGA_G
Cuts at: 3339 5822 6834 3339
Size: 2483 1012 4267
Fragments arranged by size:
4267 2483 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7762
BstBI TT'CG_AA
Cuts at: 6566 6566
Size: 7762
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7762
BstXI CCAn_nnnn'nTGG
Cuts at: 3219 4736 4786 3219
Size: 1517 50 6195
Fragments arranged by size:
6195 1517 50
BstYI r'GATC_y
Cuts at: 1972 3699 3961 4852 5092 5459 5844 5861 7313
Size: 1727 262 891 240 367 385 17 1452
Cuts at: 7313 7399 7411 7757 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1727 1452 891 385 367 346 262
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2667 2785 2791 4860 513
Size: 552 1602 118 6 2069 3415
Fragments arranged by size:
3415 2069 1602 552 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4045 487
Size: 683 56 2819 4204
Fragments arranged by size:
4204 2819 683 56
BtsI GCAGTG_nn'
Cuts at: 3642 5278 6072 6092 3642
Size: 1636 794 20 5312
Fragments arranged by size:
5312 1636 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7727
DraI TTT'AAA
Cuts at: 5066 5066
Size: 7762
DraIII CAC_nnn'GTG
Cuts at: 3112 4894 3112
Size: 1782 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3689 6769 809
Size: 507 2373 3080 1802
Fragments arranged by size:
3080 2373 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2788 2812 2824
Size: 54 1425 50 275 18 958 24 12
Cuts at: 2824 3467 3798 4023 4492 4726 4996 5209 6098
Size: 643 331 225 469 234 270 213 889
Cuts at: 6098 7727 8
Size: 1629 43
Fragments arranged by size:
1629 1425 958 889 643 469 331 275
270 234 225 213 54 50 43 24
18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2812 2824 5209 1537
Size: 275 1000 12 2385 4090
Fragments arranged by size:
4090 2385 1000 275 12
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 3231 3452 3827 4523 5692 1353
Size: 1391 487 221 375 696 1169 3423
Fragments arranged by size:
3423 1391 1169 696 487 375 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3643 6349 6723 6869 387
Size: 1064 138 2054 2706 374 146 1280
Fragments arranged by size:
2706 2054 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2205 2331 2577 3001 4306 4613
Size: 393 550 23 126 246 424 1305 307
Cuts at: 4613 4763 5825 6422 7209 7477 1239
Size: 150 1062 597 787 268 1524
Fragments arranged by size:
1524 1305 1062 787 597 550 424 393
307 268 246 150 126 23
EcoO109I rG'GnC_Cy
Cuts at: 2891 3118 3403 3838 4116 4299 2891
Size: 227 285 435 278 183 6354
Fragments arranged by size:
6354 435 285 278 227 183
EcoRI G'AATT_C
Cuts at: 5071 5449 5071
Size: 378 7384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 7762
FspI TGC'GCA
Cuts at: 6268 6268
Size: 7762
HaeII r_GCGC'y
Cuts at: 1678 3001 3792 4462 6909 1678
Size: 1323 791 670 2447 2531
Fragments arranged by size:
2531 2447 1323 791 670
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 2723 2755 3305
Size: 32 135 32 705 32 414 32 550
Cuts at: 3305 3337 3647 3679 6403 6435 6477 6509 1373
Size: 32 310 32 2724 32 42 32 2626
Fragments arranged by size:
2724 2626 705 550 414 310 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3694 5106 5352 669
Size: 988 281 1756 1412 246 3079
Fragments arranged by size:
3079 1756 1412 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7762
HpaI GTT'AAC
Cuts at: 5352 5352
Size: 7762
KpnI G_GTAC'C
Cuts at: 5087 5087
Size: 7762
MfeI C'AATT_G
Cuts at: 2296 5361 2296
Size: 3065 4697
MluI A'CGCG_T
Cuts at: 7638 7638
Size: 7762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4852 6876 7060 1305
Size: 3547 2024 184 2007
Fragments arranged by size:
3547 2024 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3800 4025 4494 4728 10
Size: 54 1425 343 1968 225 469 234 3044
Fragments arranged by size:
3044 1968 1425 469 343 234 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2660 3353 5720 6079 6238 518
Size: 648 1162 332 693 2367 359 159 2042
Fragments arranged by size:
2367 2042 1162 693 648 359 332 159
MspA1I CmG'CkG
Cuts at: 1606 2132 2243 3538 3553 4585 5178 5855 7003
Size: 526 111 1295 15 1032 593 677 1148
Cuts at: 7003 7248 1606
Size: 245 2120
Fragments arranged by size:
2120 1295 1148 1032 677 593 526 245
111 15
NaeI GCC'GGC
Cuts at: 3737 3737
Size: 7762
NcoI C'CATG_G
Cuts at: 513 1065 4860 513
Size: 552 3795 3415
Fragments arranged by size:
3795 3415 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7762
NgoMIV G'CCGG_C
Cuts at: 3735 3735
Size: 7762
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7762
NotI GC'GGCC_GC
Cuts at: 5209 5209
Size: 7762
NspI r_CATG'y
Cuts at: 1132 1229 3733 5120 6665 1132
Size: 97 2504 1387 1545 2229
Fragments arranged by size:
2504 2229 1545 1387 97
PasI CC'CwG_GG
Cuts at: 1348 2102 3743 1348
Size: 754 1641 5367
Fragments arranged by size:
5367 1641 754
PciI A'CATG_T
Cuts at: 6661 6661
Size: 7762
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 6860
PfoI T'CCnGG_A
Cuts at: 5007 5007
Size: 7762
PmeI GTTT'AAAC
Cuts at: 5066 5066
Size: 7762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5831 5863 7344 7376 5831
Size: 32 1481 32 6217
Fragments arranged by size:
6217 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3838 4116 3838
Size: 278 7484
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7762
PsiI TTA'TAA
Cuts at: 5332 5332
Size: 7762
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4559 4591 4559
Size: 32 7730
PstI C_TGCA'G
Cuts at: 830 5114 830
Size: 4284 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7762
PvuII CAG'CTG
Cuts at: 2132 2243 3538 3553 4585 2132
Size: 111 1295 15 1032 5309
Fragments arranged by size:
5309 1295 1032 111 15
SalI G'TCGA_C
Cuts at: 1936 5104 1936
Size: 3168 4594
SbfI CC_TGCA'GG
Cuts at: 5114 5114
Size: 7762
ScaI AGT'ACT
Cuts at: 6010 7722 6010
Size: 1712 6050
SfcI C'TryA_G
Cuts at: 826 1045 4344 4612 4645 5110 6245 6926 7117
Size: 219 3299 268 33 465 1135 681 191
Cuts at: 7117 826
Size: 1471
Fragments arranged by size:
3299 1471 1135 681 465 268 219 191
33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7762
SmaI CCC'GGG
Cuts at: 1872 5089 1872
Size: 3217 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4153 4412 5866 6767 7029 820
Size: 197 926 2210 259 1454 901 262 1553
Fragments arranged by size:
2210 1553 1454 926 901 262 259 197
SnaBI TAC'GTA
Cuts at: 493 5553 493
Size: 5060 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7762
SphI G_CATG'C
Cuts at: 1132 5120 1132
Size: 3988 3774
SspI AAT'ATT
Cuts at: 5 52 4769 5686 5
Size: 47 4717 917 2081
Fragments arranged by size:
4717 2081 917 47
StuI AGG'CCT
Cuts at: 1517 2035 3463 4957 1517
Size: 518 1428 1494 4322
Fragments arranged by size:
4322 1494 1428 518
StyI C'CwwG_G
Cuts at: 513 1065 2124 2226 3399 3561 3801 4254 4860
Size: 552 1059 102 1173 162 240 453 606
Cuts at: 4860 5201 513
Size: 341 3074
Fragments arranged by size:
3074 1173 1059 606 552 453 341 240
162 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 2392 3728 3756 4264 4853 5005
Size: 1255 484 328 1336 28 508 589 152
Cuts at: 5005 5789 5806 5959 6144 6589 325
Size: 784 17 153 185 445 1498
Fragments arranged by size:
1498 1336 1255 784 589 508 484 445
328 185 153 152 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 6008 7720
Size: 274 80 33 51 1443 930 3101 1712
Cuts at: 7720 96
Size: 138
Fragments arranged by size:
3101 1712 1443 930 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3981 4013 7722 7754 3506
Size: 32 443 32 3709 32 3514
Fragments arranged by size:
3709 3514 443 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2939 3319 1316
Size: 1623 380 5759
Fragments arranged by size:
5759 1623 380
XbaI T'CTAG_A
Cuts at: 5098 5098
Size: 7762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2572
Size: 7762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7762
XmaI C'CCGG_G
Cuts at: 1870 5087 1870
Size: 3217 4545
XmnI GAAnn'nnTTC
Cuts at: 3288 3638 5891 3288
Size: 350 2253 5159
Fragments arranged by size:
5159 2253 350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 5570 276
Size: 53 83 186 936 3298 738 2468
Fragments arranged by size:
3298 2468 936 738 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI