Restriction map for
pFN21AE2571
(Circular) (Six-base) MAPSORT of: pFN21AE2571.seq Check: 2214 from: 1 to: 8683
pFN21AE2571 8683 bp
With 182 enzymes: SgfI *
April 14, 2015 11:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2348 2348
Size: 8683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6493 278
Size: 53 83 186 936 4957 2468
Fragments arranged by size:
4957 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6026 1937
Size: 4089 4594
Acc65I G'GTAC_C
Cuts at: 4849 6004 4849
Size: 1155 7528
AclI AA'CG_TT
Cuts at: 1563 4199 4681 6810 7183 1563
Size: 2636 482 2129 373 3063
Fragments arranged by size:
3063 2636 2129 482 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 4953 5769 6746 8130 8398 1632
Size: 559 2762 816 977 1384 268 1917
Fragments arranged by size:
2762 1917 1384 977 816 559 268
AfeI AGC'GCT
Cuts at: 4839 4839
Size: 8683
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8486
AflIII A'CryG_T
Cuts at: 1203 3072 7582 8559 1203
Size: 1869 4510 977 1327
Fragments arranged by size:
4510 1869 1327 977
AgeI A'CCGG_T
Cuts at: 7514 7514
Size: 8683
AhdI GACnn_n'nnGTC
Cuts at: 7412 7412
Size: 8683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5519 1184
Size: 4335 4348
AlwNI CAG_nnn'CTG
Cuts at: 2434 3393 6426 7998 2434
Size: 959 3033 1572 3119
Fragments arranged by size:
3119 3033 1572 959
ApaI G_GGCC'C
Cuts at: 2468 2468
Size: 8683
ApaLI G'TGCA_C
Cuts at: 6740 7896 6740
Size: 1156 7527
ApoI r'AATT_y
Cuts at: 1493 2079 2587 2935 3559 4210 5087 5992 6218
Size: 586 508 348 624 651 877 905 226
Cuts at: 6218 6370 8479 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 905 877 651 624 586 508
348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7237 160
Size: 872 6205 1606
Fragments arranged by size:
6205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8683
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2303 4906 6008 1870
Size: 73 252 108 2603 1102 4545
Fragments arranged by size:
4545 2603 1102 252 108 73
BamHI G'GATC_C
Cuts at: 6013 6380 6013
Size: 367 8316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 4849 6004 7459 618
Size: 325 217 627 549 2513 1155 1455 1842
Fragments arranged by size:
2513 1842 1455 1155 627 549 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2468 3160 3324 4907 5612 5633 6002
Size: 630 447 692 164 1583 705 21 369
Cuts at: 6002 1391
Size: 4072
Fragments arranged by size:
4072 1583 705 692 630 447 369 164
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3205 3963 4872 928
Size: 971 1306 758 909 4739
Fragments arranged by size:
4739 1306 971 909 758
BbvCI CC'TCA_GC
Cuts at: 4662 4662
Size: 8683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6874 6908 1903
Size: 34 4937 34 3678
Fragments arranged by size:
4937 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2545 4729 4784 6575 7785 2545
Size: 2184 55 1791 1210 3443
Fragments arranged by size:
3443 2184 1791 1210 55
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5928 7294 136
Size: 107 122 71 631 605 4256 1366 1525
Fragments arranged by size:
4256 1525 1366 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2715 4729 8678 2715
Size: 2014 3949 2720
Fragments arranged by size:
3949 2720 2014
BlpI GC'TnA_GC
Cuts at: 2457 6100 2457
Size: 3643 5040
Bme1580I G_kGCm'C
Cuts at: 1790 2468 6744 7900 1790
Size: 678 4276 1156 2573
Fragments arranged by size:
4276 2573 1156 678
BmgBI CAC'GTC
Cuts at: 1318 2311 4616 1318
Size: 993 2305 5385
Fragments arranged by size:
5385 2305 993
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5655 6414 7372
Size: 416 53 83 399 24 4238 759 958
Cuts at: 7372 442
Size: 1753
Fragments arranged by size:
4238 1753 958 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 3080 3179 4945 2035
Size: 1045 99 1766 5773
Fragments arranged by size:
5773 1766 1045 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2411 2888 3187 4317 5581 5893 7343
Size: 1116 56 477 299 1130 1264 312 1450
Cuts at: 7343 1239
Size: 2579
Fragments arranged by size:
2579 1450 1264 1130 1116 477 312 299
56
Bpu10I CC'TnA_GC
Cuts at: 3318 3806 4662 3318
Size: 488 856 7339
Fragments arranged by size:
7339 856 488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 3402 3656 3909 4361 6808 7673 7971 2740
Size: 662 254 253 452 2447 865 298 3452
Fragments arranged by size:
3452 2447 865 662 452 298 254 253
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 5060 5607 7346 882
Size: 617 2772 789 547 1739 2219
Fragments arranged by size:
2772 2219 1739 789 617 547
BsaAI yAC'GTr
Cuts at: 493 1204 4448 6474 493
Size: 711 3244 2026 2702
Fragments arranged by size:
3244 2702 2026 711
BsaBI GATnn'nnATC
Cuts at: 3287 5922 8677 3287
Size: 2635 2755 3293
Fragments arranged by size:
3293 2755 2635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2297 6490 6872
Size: 53 83 186 936 696 68 4193 382
Cuts at: 6872 7507 275
Size: 635 1451
Fragments arranged by size:
4193 1451 936 696 635 382 186 83
68 53
BsaWI w'CCGG_w
Cuts at: 3222 7116 7514 7788 7935 8346 3222
Size: 3894 398 274 147 411 3559
Fragments arranged by size:
3894 3559 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 1178
Size: 30 165 30 706 30 7722
Fragments arranged by size:
7722 706 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 4679 4715 5184 5272 5390 1186
Size: 841 2652 36 469 88 118 4479
Fragments arranged by size:
4479 2652 841 469 118 88 36
BseYI C'CCAG_C
Cuts at: 3090 4065 5817 5822 5862 7886 8382 3090
Size: 975 1752 5 40 2024 496 3391
Fragments arranged by size:
3391 2024 1752 975 496 40 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2296 3825 1163
Size: 573 560 1529 6021
Fragments arranged by size:
6021 1529 573 560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6133 6894 7922 1540
Size: 275 186 4132 761 1028 2301
Fragments arranged by size:
4132 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2812 3160 3195 3418 4907 5352 5612 6744
Size: 791 348 35 223 1489 445 260 1132
Cuts at: 6744 6829 7900 2021
Size: 85 1071 2804
Fragments arranged by size:
2804 1489 1132 1071 791 445 348 260
223 85 35
BsmI GAATG_Cn'
Cuts at: 4791 6194 6287 4791
Size: 1403 93 7187
Fragments arranged by size:
7187 1403 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4128 4128
Size: 8683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2468 2812 3160 3195 3324 3418
Size: 399 231 447 344 348 35 129 94
Cuts at: 3418 4907 5352 5612 5633 6002 6744 6829 7900
Size: 1489 445 260 21 369 742 85 1071
Cuts at: 7900 1391
Size: 2174
Fragments arranged by size:
2174 1489 1071 742 447 445 399 369
348 344 260 231 129 94 85 35
21
BspEI T'CCGG_A
Cuts at: 8346 8346
Size: 8683
BspHI T'CATG_A
Cuts at: 2382 6572 8302 2382
Size: 4190 1730 2763
Fragments arranged by size:
4190 2763 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2348 3993 6038 844
Size: 1504 1645 2045 3489
Fragments arranged by size:
3489 2045 1645 1504
BsrBI CCG'CTC
Cuts at: 1394 1823 4168 6570 1394
Size: 429 2345 2402 3507
Fragments arranged by size:
3507 2402 2345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2634 3435 4579 7178 7352 65
Size: 1183 1386 801 1144 2599 174 1396
Fragments arranged by size:
2599 1396 1386 1183 1144 801 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7327 7514 1700
Size: 110 5517 187 2869
Fragments arranged by size:
5517 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8683
BssSI C'ACGA_G
Cuts at: 3998 6743 7755 3998
Size: 2745 1012 4926
Fragments arranged by size:
4926 2745 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8683
BstBI TT'CG_AA
Cuts at: 7487 7487
Size: 8683
BstEII G'GTnAC_C
Cuts at: 1184 3682 5104 1184
Size: 2498 1422 4763
Fragments arranged by size:
4763 2498 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2746 4226 2293
Size: 453 1480 6750
Fragments arranged by size:
6750 1480 453
BstYI r'GATC_y
Cuts at: 1972 2683 2715 3382 4729 6013 6380 6765 6782
Size: 711 32 667 1347 1284 367 385 17
Cuts at: 6782 8234 8320 8332 8678 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1347 1284 711 667 385 367
346 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2345 2345
Size: 8683
BtgI C'CryG_G
Cuts at: 513 1065 2397 2451 3166 4347 513
Size: 552 1332 54 715 1181 4849
Fragments arranged by size:
4849 1332 1181 715 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7944
Fragments arranged by size:
7944 683 56
BtsI GCAGTG_nn'
Cuts at: 3785 3837 5503 6199 6993 7013 3785
Size: 52 1666 696 794 20 5455
Fragments arranged by size:
5455 1666 794 696 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8648
DraI TTT'AAA
Cuts at: 4155 5987 4155
Size: 1832 6851
DraIII CAC_nnn'GTG
Cuts at: 2993 2993
Size: 8683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7690 809
Size: 507 6374 1802
Fragments arranged by size:
6374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2802 3777 5803
Size: 54 1425 50 275 18 972 975 2026
Cuts at: 5803 5920 6130 7019 8648 8
Size: 117 210 889 1629 43
Fragments arranged by size:
2026 1629 1425 975 972 889 275 210
117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6130 1537
Size: 275 4318 4090
Fragments arranged by size:
4318 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3165 4763 4839 4929 5397 6613 1353
Size: 1812 1598 76 90 468 1216 3423
Fragments arranged by size:
3423 1812 1598 1216 468 90 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5485 7270 7644 7790 387
Size: 1064 138 3896 1785 374 146 1280
Fragments arranged by size:
3896 1785 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2019 3158 4905 5610 2019
Size: 1139 1747 705 5092
Fragments arranged by size:
5092 1747 1139 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2355 2411 2888 3187 4317 4953
Size: 393 559 164 56 477 299 1130 636
Cuts at: 4953 5581 5769 5893 6746 7343 8130 8398 1239
Size: 628 188 124 853 597 787 268 1524
Fragments arranged by size:
1524 1130 853 787 636 628 597 559
477 393 299 268 188 164 124 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2344 4040 4889 2344
Size: 1696 849 6138
Fragments arranged by size:
6138 1696 849
EcoO109I rG'GnC_Cy
Cuts at: 2464 2465 2620 2464
Size: 1 155 8527
Fragments arranged by size:
8527 155 1
EcoRI G'AATT_C
Cuts at: 5992 6370 5992
Size: 378 8305
EcoRV GAT'ATC
Cuts at: 4492 4492
Size: 8683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5771 5771
Size: 8683
FspI TGC'GCA
Cuts at: 7189 7189
Size: 8683
HaeII r_GCGC'y
Cuts at: 1678 2416 2435 4841 7830 1678
Size: 738 19 2406 2989 2531
Fragments arranged by size:
2989 2531 2406 738 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3050 3082 3389 3421 3617
Size: 32 135 32 1478 32 307 32 196
Cuts at: 3617 3649 4125 4142 4157 4174 4559 4591 4615
Size: 32 476 17 15 17 385 32 24
Cuts at: 4615 4647 5720 5752 7324 7356 7398 7430 1373
Size: 32 1073 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 1478 1073 476 385 307 196
135 42 32 32 32 32 32 32
32 32 32 32 24 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2202 5133 6027 6273 669
Size: 988 281 264 2931 894 246 3079
Fragments arranged by size:
3079 2931 988 894 281 264 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8683
HpaI GTT'AAC
Cuts at: 2202 6273 2202
Size: 4071 4612
KpnI G_GTAC'C
Cuts at: 4853 6008 4853
Size: 1155 7528
MfeI C'AATT_G
Cuts at: 6282 6282
Size: 8683
MluI A'CGCG_T
Cuts at: 8559 8559
Size: 8683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2742 4452 7797 7981 1305
Size: 780 657 1710 3345 184 2007
Fragments arranged by size:
3345 2007 1710 780 657 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 3779 5805 5922 10
Size: 54 1425 343 972 975 2026 117 2771
Fragments arranged by size:
2771 2026 1425 975 972 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2291 4224 4694 6641 7000 7159
Size: 648 1119 6 1933 470 1947 359 159
Cuts at: 7159 518
Size: 2042
Fragments arranged by size:
2042 1947 1933 1119 648 470 359 159
6
MspA1I CmG'CkG
Cuts at: 1606 5009 6099 6776 7924 8169 1606
Size: 3403 1090 677 1148 245 2120
Fragments arranged by size:
3403 2120 1148 1090 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2397 4347 513
Size: 552 1332 1950 4849
Fragments arranged by size:
4849 1950 1332 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8683
NotI GC'GGCC_GC
Cuts at: 6130 6130
Size: 8683
NspI r_CATG'y
Cuts at: 1132 1229 3076 6041 7586 1132
Size: 97 1847 2965 1545 2229
Fragments arranged by size:
2965 2229 1847 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2031 3703 1348
Size: 683 1672 6328
Fragments arranged by size:
6328 1672 683
PciI A'CATG_T
Cuts at: 3072 7582 3072
Size: 4510 4173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 5127 5491 1108
Size: 1344 2675 364 4300
Fragments arranged by size:
4300 2675 1344 364
PfoI T'CCnGG_A
Cuts at: 4143 4563 4143
Size: 420 8263
PmeI GTTT'AAAC
Cuts at: 5987 5987
Size: 8683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6752 6784 8265 8297 6752
Size: 32 1481 32 7138
Fragments arranged by size:
7138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2620 2620
Size: 8683
PshAI GACnn'nnGTC
Cuts at: 1613 3680 1613
Size: 2067 6616
PsiI TTA'TAA
Cuts at: 6253 6253
Size: 8683
PspOMI G'GGCC_C
Cuts at: 2464 2464
Size: 8683
PspXI vC'TCGA_Gb
Cuts at: 2195 2195
Size: 8683
PstI C_TGCA'G
Cuts at: 830 3645 3792 4041 5649 6035 830
Size: 2815 147 249 1608 386 3478
Fragments arranged by size:
3478 2815 1608 386 249 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8683
SacI G_AGCT'C
Cuts at: 2021 3160 4907 5612 2021
Size: 1139 1747 705 5092
Fragments arranged by size:
5092 1747 1139 705
SalI G'TCGA_C
Cuts at: 1936 6025 1936
Size: 4089 4594
SapI GCTCTTCn'nnn_
Cuts at: 3165 3165
Size: 8683
SbfI CC_TGCA'GG
Cuts at: 6035 6035
Size: 8683
ScaI AGT'ACT
Cuts at: 6931 8643 6931
Size: 1712 6971
SfcI C'TryA_G
Cuts at: 826 1045 3641 3788 4037 5645 6031 7166 7847
Size: 219 2596 147 249 1608 386 1135 681
Cuts at: 7847 8038 826
Size: 191 1471
Fragments arranged by size:
2596 1608 1471 1135 681 386 249 219
191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8683
SmaI CCC'GGG
Cuts at: 1872 6010 1872
Size: 4138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 2719 3417 3671 3924 4340
Size: 197 926 252 524 698 254 253 416
Cuts at: 4340 6787 7688 7950 820
Size: 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 698 524 416 262
254 253 252 197
SnaBI TAC'GTA
Cuts at: 493 6474 493
Size: 5981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8683
SphI G_CATG'C
Cuts at: 1132 6041 1132
Size: 4909 3774
SspI AAT'ATT
Cuts at: 5 52 3182 6607 5
Size: 47 3130 3425 2081
Fragments arranged by size:
3425 3130 2081 47
StuI AGG'CCT
Cuts at: 1517 4028 1517
Size: 2511 6172
StyI C'CwwG_G
Cuts at: 513 1065 2397 4347 6122 513
Size: 552 1332 1950 1775 3074
Fragments arranged by size:
3074 1950 1775 1332 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 4079 5015 6710 6727 6880 7065
Size: 1255 444 2055 936 1695 17 153 185
Cuts at: 7065 7510 325
Size: 445 1498
Fragments arranged by size:
2055 1695 1498 1255 936 445 444 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2536 3610 4096
Size: 274 80 33 51 1443 559 1074 486
Cuts at: 4096 6929 8641 96
Size: 2833 1712 138
Fragments arranged by size:
2833 1712 1443 1074 559 486 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5191 5223 8643 8675 5191
Size: 32 3420 32 5199
Fragments arranged by size:
5199 3420 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3146 1316
Size: 1830 6853
XbaI T'CTAG_A
Cuts at: 4828 6019 4828
Size: 1191 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3109 4693 5662 3109
Size: 1584 969 6130
Fragments arranged by size:
6130 1584 969
XhoI C'TCGA_G
Cuts at: 1943 2195 1943
Size: 252 8431
XmaI C'CCGG_G
Cuts at: 1870 6008 1870
Size: 4138 4545
XmnI GAAnn'nnTTC
Cuts at: 6812 6812
Size: 8683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6491 276
Size: 53 83 186 936 4957 2468
Fragments arranged by size:
4957 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI