Restriction map for
pFN21AE2558
(Circular) (Six-base) MAPSORT of: pFN21AE2558.seq Check: 8087 from: 1 to: 7075
pFN21AE2558 7075 bp
With 182 enzymes: SgfI *
December 25, 2014 18:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2196 2196
Size: 7075
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4885 278
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4254 4356 4418 1937
Size: 2317 102 62 4594
Fragments arranged by size:
4594 2317 102 62
Acc65I G'GTAC_C
Cuts at: 4170 4396 4170
Size: 226 6849
AclI AA'CG_TT
Cuts at: 1563 5202 5575 1563
Size: 3639 373 3063
Fragments arranged by size:
3639 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 3460 3644 5138 6522 6790 1632
Size: 980 848 184 1494 1384 268 1917
Fragments arranged by size:
1917 1494 1384 980 848 268 184
AfeI AGC'GCT
Cuts at: 2486 2486
Size: 7075
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6878
AflIII A'CryG_T
Cuts at: 1203 4113 5974 6951 1203
Size: 2910 1861 977 1327
Fragments arranged by size:
2910 1861 1327 977
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 3974 5804 3974
Size: 1830 5245
AleI CACnn'nnGTG
Cuts at: 1166 2297 2882 1166
Size: 1131 585 5359
Fragments arranged by size:
5359 1131 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4067 4216 1184
Size: 2883 149 4043
Fragments arranged by size:
4043 2883 149
AlwNI CAG_nnn'CTG
Cuts at: 2239 2248 2431 2527 3868 3970 4818 6390 2239
Size: 9 183 96 1341 102 848 1572 2924
Fragments arranged by size:
2924 1572 1341 848 183 102 96 9
ApaI G_GGCC'C
Cuts at: 2170 2550 2170
Size: 380 6695
ApaLI G'TGCA_C
Cuts at: 3926 4316 5132 6288 3926
Size: 390 816 1156 4713
Fragments arranged by size:
4713 1156 816 390
ApoI r'AATT_y
Cuts at: 1493 4384 4610 4762 6871 1493
Size: 2891 226 152 2109 1697
Fragments arranged by size:
2891 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5629 160
Size: 872 4597 1606
Fragments arranged by size:
4597 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2157 3164 4073 4400 1870
Size: 73 197 17 1007 909 327 4545
Fragments arranged by size:
4545 1007 909 327 197 73 17
BamHI G'GATC_C
Cuts at: 2685 4405 4772 2685
Size: 1720 367 4988
Fragments arranged by size:
4988 1720 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2323 2602 2646 2932
Size: 325 217 627 312 224 279 44 286
Cuts at: 2932 3542 4170 4345 4396 5851 618
Size: 610 628 175 51 1455 1842
Fragments arranged by size:
1842 1455 628 627 610 325 312 286
279 224 217 175 51 44
BanII G_rGCy'C
Cuts at: 1391 2170 2217 2256 2550 3444 4290 4394 1391
Size: 779 47 39 294 894 846 104 4072
Fragments arranged by size:
4072 894 846 779 294 104 47 39
BbeI G_GCGC'C
Cuts at: 2103 2327 3546 2103
Size: 224 1219 5632
Fragments arranged by size:
5632 1219 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3550 928
Size: 971 1651 4453
Fragments arranged by size:
4453 1651 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5266 5300 1903
Size: 34 50 34 3245 34 3678
Fragments arranged by size:
3678 3245 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4967 6177 4967
Size: 1210 5865
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2133 5686 136
Size: 107 122 71 631 605 461 3553 1525
Fragments arranged by size:
3553 1525 631 605 461 122 107 71
BglII A'GATC_T
Cuts at: 4143 7070 4143
Size: 2927 4148
BlpI GC'TnA_GC
Cuts at: 3669 4492 3669
Size: 823 6252
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2470 2550 2586 2607 3930 4320 5136
Size: 380 300 80 36 21 1323 390 816
Cuts at: 5136 6292 1790
Size: 1156 2573
Fragments arranged by size:
2573 1323 1156 816 390 380 300 80
36 21
BmgBI CAC'GTC
Cuts at: 1318 2635 2796 3064 4114 4217 1318
Size: 1317 161 268 1050 103 4176
Fragments arranged by size:
4176 1317 1050 268 161 103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2513 4806 5764
Size: 416 53 83 399 24 1096 2293 958
Cuts at: 5764 442
Size: 1753
Fragments arranged by size:
2293 1753 1096 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3882 1056
Size: 2826 4249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2405 2690 2720 3023 3219 3342 3825
Size: 1031 135 285 30 303 196 123 483
Cuts at: 3825 5735 1239
Size: 1910 2579
Fragments arranged by size:
2579 1910 1031 483 303 285 196 135
123 30
Bpu10I CC'TnA_GC
Cuts at: 3588 3588
Size: 7075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 5200 6065 6363 3506
Size: 1694 865 298 4218
Fragments arranged by size:
4218 1694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 2836 2969 3014 3195 3244 5738
Size: 617 816 521 133 45 181 49 2494
Cuts at: 5738 882
Size: 2219
Fragments arranged by size:
2494 2219 816 617 521 181 133 49
45
BsaAI yAC'GTr
Cuts at: 493 1204 4235 4866 493
Size: 711 3031 631 2702
Fragments arranged by size:
3031 2702 711 631
BsaBI GATnn'nnATC
Cuts at: 7069 7069
Size: 7075
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 2324 3543 3632
Size: 53 83 186 936 567 224 1219 89
Cuts at: 3632 3717 4882 5264 5899 275
Size: 85 1165 382 635 1451
Fragments arranged by size:
1451 1219 1165 936 635 567 382 224
186 89 85 83 53
BsaWI w'CCGG_w
Cuts at: 5508 5906 6180 6327 6738 5508
Size: 398 274 147 411 5845
Fragments arranged by size:
5845 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3192 3222 4163 4193 1178
Size: 30 165 30 1789 30 941 30 4060
Fragments arranged by size:
4060 1789 941 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2337 2507 2510 1186
Size: 942 209 170 3 5751
Fragments arranged by size:
5751 942 209 170 3
BseYI C'CCAG_C
Cuts at: 2046 2487 2799 3532 4290 6278 6774 2046
Size: 441 312 733 758 1988 496 2347
Fragments arranged by size:
2347 1988 758 733 496 441 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 2438 2858 2888 3706 1163
Size: 573 693 9 420 30 818 4532
Fragments arranged by size:
4532 818 693 573 420 30 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 5286 6314 1540
Size: 275 186 2524 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2256 2970 3303 3522 3646 3930 4137 4320
Size: 39 714 333 219 124 284 207 183
Cuts at: 4320 5136 5221 6292 2217
Size: 816 85 1071 3000
Fragments arranged by size:
3000 1071 816 714 333 284 219 207
183 124 85 39
BsmI GAATG_Cn'
Cuts at: 4586 4679 4586
Size: 93 6982
BsmBI CGTCTCn'nnnn_
Cuts at: 2854 2943 2854
Size: 89 6986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2217 2256 2470 2550 2586 2607
Size: 399 380 47 39 214 80 36 21
Cuts at: 2607 2970 3303 3444 3522 3646 3930 4137 4290
Size: 363 333 141 78 124 284 207 153
Cuts at: 4290 4320 4394 5136 5221 6292 1391
Size: 30 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 363 333 284
214 207 153 141 124 85 80 78
74 47 39 36 30 21
BspEI T'CCGG_A
Cuts at: 6738 6738
Size: 7075
BspHI T'CATG_A
Cuts at: 3390 4964 6694 3390
Size: 1574 1730 3771
Fragments arranged by size:
3771 1730 1574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 4430 844
Size: 1352 2234 3489
Fragments arranged by size:
3489 2234 1352
BsrBI CCG'CTC
Cuts at: 1394 1823 4228 4962 1394
Size: 429 2405 734 3507
Fragments arranged by size:
3507 2405 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5570 5744 65
Size: 1183 4322 174 1396
Fragments arranged by size:
4322 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2678 2929 3007 5719 5906 1700
Size: 110 868 251 78 2712 187 2869
Fragments arranged by size:
2869 2712 868 251 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7075
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7075
BssSI C'ACGA_G
Cuts at: 5135 6147 5135
Size: 1012 6063
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 3703 3925 794
Size: 1445 1464 222 3944
Fragments arranged by size:
3944 1464 1445 222
BstBI TT'CG_AA
Cuts at: 5879 5879
Size: 7075
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7075
BstXI CCAn_nnnn'nTGG
Cuts at: 3817 3817
Size: 7075
BstYI r'GATC_y
Cuts at: 1972 2685 4143 4405 4772 5157 5174 6626 6712
Size: 713 1458 262 367 385 17 1452 86
Cuts at: 6712 6724 7070 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1458 1452 713 385 367 346 262
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2131 2712 3796 513
Size: 552 1066 581 1084 3792
Fragments arranged by size:
3792 1084 1066 581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 487
Size: 683 56 938 5398
Fragments arranged by size:
5398 938 683 56
BtsI GCAGTG_nn'
Cuts at: 2537 4591 5385 5405 2537
Size: 2054 794 20 4207
Fragments arranged by size:
4207 2054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3292 3327 529
Size: 35 2728 35 4277
Fragments arranged by size:
4277 2728 35 35
DraI TTT'AAA
Cuts at: 4379 4379
Size: 7075
DraIII CAC_nnn'GTG
Cuts at: 3703 3703
Size: 7075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6082 809
Size: 507 4766 1802
Fragments arranged by size:
4766 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2757 2859 3106
Size: 54 1425 50 275 18 927 102 247
Cuts at: 3106 3258 3372 3939 4229 4522 5411 7040 8
Size: 152 114 567 290 293 889 1629 43
Fragments arranged by size:
1629 1425 927 889 567 293 290 275
247 152 114 102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 2957 3281 5005 1353
Size: 944 660 324 1724 3423
Fragments arranged by size:
3423 1724 944 660 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2031 2523 3106 5662 6036 6182
Size: 1064 138 442 492 583 2556 374 146
Cuts at: 6182 387
Size: 1280
Fragments arranged by size:
2556 1280 1064 583 492 442 374 146
138
EcoICRI GAG'CTC
Cuts at: 2215 2254 2215
Size: 39 7036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2405 2612 2690 2720 3023 3219
Size: 393 638 135 207 78 30 303 196
Cuts at: 3219 3342 3460 3644 3825 5138 5735 6522 6790
Size: 123 118 184 181 1313 597 787 268
Cuts at: 6790 1239
Size: 1524
Fragments arranged by size:
1524 1313 787 638 597 393 303 268
207 196 184 181 135 123 118 78
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2672 4340 2672
Size: 1668 5407
EcoO109I rG'GnC_Cy
Cuts at: 2166 2167 2269 2413 2546 2547 2665 3169 3772
Size: 1 102 144 133 1 118 504 603
Cuts at: 3772 3845 3934 2166
Size: 73 89 5307
Fragments arranged by size:
5307 603 504 144 133 118 102 89
73 1 1
EcoRI G'AATT_C
Cuts at: 4384 4762 4384
Size: 378 6697
EcoRV GAT'ATC
Cuts at: 4009 4009
Size: 7075
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 2103 2327 2488 3508 3546 6222 1678
Size: 425 224 161 1020 38 2676 2531
Fragments arranged by size:
2676 2531 1020 425 224 161 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3960 3992 5716 5748 5790
Size: 32 135 32 2388 32 1724 32 42
Cuts at: 5790 5822 1373
Size: 32 2626
Fragments arranged by size:
2626 2388 1724 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 3067 4419 4665 669
Size: 988 281 907 222 1352 246 3079
Fragments arranged by size:
3079 1352 988 907 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7075
HpaI GTT'AAC
Cuts at: 4665 4665
Size: 7075
KasI G'GCGC_C
Cuts at: 2099 2323 3542 2099
Size: 224 1219 5632
Fragments arranged by size:
5632 1219 224
KpnI G_GTAC'C
Cuts at: 4174 4400 4174
Size: 226 6849
MfeI C'AATT_G
Cuts at: 4674 4674
Size: 7075
MluI A'CGCG_T
Cuts at: 6951 6951
Size: 7075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4203 4206 6189 6373 1305
Size: 2898 3 1983 184 2007
Fragments arranged by size:
2898 2007 1983 184 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 3374 3941 10
Size: 54 1425 343 927 615 567 3144
Fragments arranged by size:
3144 1425 927 615 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2717 2882 5033 5392 5551 518
Size: 648 1131 420 165 2151 359 159 2042
Fragments arranged by size:
2151 2042 1131 648 420 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2574 2872 3865 4282 4491 5168 6316
Size: 440 528 298 993 417 209 677 1148
Cuts at: 6316 6561 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 993 677 528 440 417 298
245 209
NaeI GCC'GGC
Cuts at: 2680 2931 3009 2680
Size: 251 78 6746
Fragments arranged by size:
6746 251 78
NarI GG'CG_CC
Cuts at: 2100 2324 3543 2100
Size: 224 1219 5632
Fragments arranged by size:
5632 1219 224
NcoI C'CATG_G
Cuts at: 513 1065 3796 513
Size: 552 2731 3792
Fragments arranged by size:
3792 2731 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7075
NgoMIV G'CCGG_C
Cuts at: 2678 2929 3007 2678
Size: 251 78 6746
Fragments arranged by size:
6746 251 78
NheI G'CTAG_C
Cuts at: 1052 3878 1052
Size: 2826 4249
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 2563 4433 5978 1132
Size: 97 1334 1870 1545 2229
Fragments arranged by size:
2229 1870 1545 1334 97
PasI CC'CwG_GG
Cuts at: 1348 2743 3850 1348
Size: 1395 1107 4573
Fragments arranged by size:
4573 1395 1107
PciI A'CATG_T
Cuts at: 5974 5974
Size: 7075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3365 3970 4062 1108
Size: 2257 605 92 4121
Fragments arranged by size:
4121 2257 605 92
PfoI T'CCnGG_A
Cuts at: 2396 3000 2396
Size: 604 6471
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PmlI CAC'GTG
Cuts at: 4235 4235
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5144 5176 6657 6689 5144
Size: 32 1481 32 5530
Fragments arranged by size:
5530 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3169 3772 3934 3169
Size: 603 162 6310
Fragments arranged by size:
6310 603 162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7075
PsiI TTA'TAA
Cuts at: 4645 4645
Size: 7075
PspOMI G'GGCC_C
Cuts at: 2166 2546 2166
Size: 380 6695
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3455 3487 4079 4111 3455
Size: 32 592 32 6419
Fragments arranged by size:
6419 592 32 32
PstI C_TGCA'G
Cuts at: 830 2244 2532 3690 4427 830
Size: 1414 288 1158 737 3478
Fragments arranged by size:
3478 1414 1158 737 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
PvuII CAG'CTG
Cuts at: 2046 2574 2872 3865 2046
Size: 528 298 993 5256
Fragments arranged by size:
5256 993 528 298
SacI G_AGCT'C
Cuts at: 2217 2256 2217
Size: 39 7036
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SapI GCTCTTCn'nnn_
Cuts at: 2957 2957
Size: 7075
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 5323 7035 5323
Size: 1712 5363
SfcI C'TryA_G
Cuts at: 826 1045 2240 2480 2528 3686 4012 4423 5558
Size: 219 1195 240 48 1158 326 411 1135
Cuts at: 5558 6239 6430 826
Size: 681 191 1471
Fragments arranged by size:
1471 1195 1158 1135 681 411 326 240
219 191 48
SfoI GGC'GCC
Cuts at: 2101 2325 3544 2101
Size: 224 1219 5632
Fragments arranged by size:
5632 1219 224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7075
SmaI CCC'GGG
Cuts at: 1872 2159 4402 1872
Size: 287 2243 4545
Fragments arranged by size:
4545 2243 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3521 5179 6080 6342 820
Size: 197 926 1578 1658 901 262 1553
Fragments arranged by size:
1658 1578 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4866 493
Size: 4373 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7075
SphI G_CATG'C
Cuts at: 1132 4433 1132
Size: 3301 3774
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 3113 1517
Size: 1596 5479
StyI C'CwwG_G
Cuts at: 513 1065 3445 3796 4514 513
Size: 552 2380 351 718 3074
Fragments arranged by size:
3074 2380 718 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3074 3389 5102 5119 5272 5457 5902
Size: 1255 1494 315 1713 17 153 185 445
Cuts at: 5902 325
Size: 1498
Fragments arranged by size:
1713 1498 1494 1255 445 315 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5321 7033 96
Size: 274 80 33 51 1443 3344 1712 138
Fragments arranged by size:
3344 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3192 3224 7035 7067 3192
Size: 32 3811 32 3200
Fragments arranged by size:
3811 3200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7075
XbaI T'CTAG_A
Cuts at: 4411 4411
Size: 7075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 2614
Size: 7075
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7075
XmaI C'CCGG_G
Cuts at: 1870 2157 4400 1870
Size: 287 2243 4545
Fragments arranged by size:
4545 2243 287
XmnI GAAnn'nnTTC
Cuts at: 3557 5204 3557
Size: 1647 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4883 276
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I FalI FseI FspAI NruI NsiI PacI PspXI
RsrII SacII SanDI SexAI SfiI SrfI SwaI