Restriction map for
pFN21AE2551
(Circular) (Six-base) MAPSORT of: pFN21AE2551.seq Check: 2841 from: 1 to: 6595
pFN21AE2551 6595 bp
With 182 enzymes: SgfI *
August 28, 2015 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3428 3428
Size: 6595
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4405 278
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3938 1937
Size: 2001 4594
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2709 2791 3580 4658 6042 6310 1632
Size: 1077 82 789 1078 1384 268 1917
Fragments arranged by size:
1917 1384 1078 1077 789 268 82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 3370 5494 6471 1203
Size: 2167 2124 977 1327
Fragments arranged by size:
2167 2124 1327 977
AgeI A'CCGG_T
Cuts at: 2724 5426 2724
Size: 2702 3893
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 2213 3350 1166
Size: 1047 1137 4411
Fragments arranged by size:
4411 1137 1047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6595
AlwNI CAG_nnn'CTG
Cuts at: 2746 2814 3536 4338 5910 2746
Size: 68 722 802 1572 3431
Fragments arranged by size:
3431 1572 802 722 68
ApaI G_GGCC'C
Cuts at: 3501 3501
Size: 6595
ApaLI G'TGCA_C
Cuts at: 4652 5808 4652
Size: 1156 5439
ApoI r'AATT_y
Cuts at: 1493 2023 2693 3861 3904 4130 4282 6391 1493
Size: 530 670 1168 43 226 152 2109 1697
Fragments arranged by size:
2109 1697 1168 670 530 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 5149 160
Size: 872 4117 1606
Fragments arranged by size:
4117 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 3601 3920 1870
Size: 73 1658 319 4545
Fragments arranged by size:
4545 1658 319 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3709 3742 3709
Size: 33 6562
BamHI G'GATC_C
Cuts at: 3431 3925 4292 3431
Size: 494 367 5734
Fragments arranged by size:
5734 494 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3916 5371 618
Size: 325 217 627 1113 1016 1455 1842
Fragments arranged by size:
1842 1455 1113 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2415 2739 3501 3683 3914 1391
Size: 1024 324 762 182 231 4072
Fragments arranged by size:
4072 1024 762 324 231 182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2424 2458 4786 4820 1903
Size: 34 487 34 2328 34 3678
Fragments arranged by size:
3678 2328 487 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 3342 3795 1548
Size: 1794 453 4348
Fragments arranged by size:
4348 1794 453
BfrBI ATG'CAT
Cuts at: 2445 2445
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5206 136
Size: 107 122 71 631 605 3534 1525
Fragments arranged by size:
3534 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3000 6590 3000
Size: 3590 3005
BlpI GC'TnA_GC
Cuts at: 2220 4012 2220
Size: 1792 4803
Bme1580I G_kGCm'C
Cuts at: 1790 3501 4656 5812 1790
Size: 1711 1155 1156 2573
Fragments arranged by size:
2573 1711 1156 1155
BmgBI CAC'GTC
Cuts at: 1318 2679 2710 3373 3577 1318
Size: 1361 31 663 204 4336
Fragments arranged by size:
4336 1361 663 204 31
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2429 4326 5284
Size: 416 53 83 399 24 1012 1897 958
Cuts at: 5284 442
Size: 1753
Fragments arranged by size:
1897 1753 1012 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3051 1056
Size: 1995 4600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 2934 2941 2987 3500 3634 3742 3789
Size: 1484 211 7 46 513 134 108 47
Cuts at: 3789 5255 1239
Size: 1466 2579
Fragments arranged by size:
2579 1484 1466 513 211 134 108 47
46 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4720 5585 5883 4720
Size: 865 298 5432
Fragments arranged by size:
5432 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 5258 882
Size: 617 738 3021 2219
Fragments arranged by size:
3021 2219 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 3466 4386 493
Size: 711 2027 235 920 2702
Fragments arranged by size:
2702 2027 920 711 235
BsaBI GATnn'nnATC
Cuts at: 3296 6589 3296
Size: 3293 3302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2823 4402 4784
Size: 53 83 186 936 482 808 1579 382
Cuts at: 4784 5419 275
Size: 635 1451
Fragments arranged by size:
1579 1451 936 808 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2724 5028 5426 5700 5847 6258 2724
Size: 2304 398 274 147 411 3061
Fragments arranged by size:
3061 2304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 1178
Size: 30 165 30 1149 30 5191
Fragments arranged by size:
5191 1149 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2560 3209 3497 1186
Size: 1162 212 649 288 4284
Fragments arranged by size:
4284 1162 649 288 212
BseYI C'CCAG_C
Cuts at: 2194 3212 3304 3624 5798 6294 2194
Size: 1018 92 320 2174 496 2495
Fragments arranged by size:
2495 2174 1018 496 320 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2614 1163
Size: 573 878 5144
Fragments arranged by size:
5144 878 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4045 4806 5834 1540
Size: 275 186 2044 761 1028 2301
Fragments arranged by size:
2301 2044 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 3153 3405 3510 4656 4741 5812 2415
Size: 738 252 105 1146 85 1071 3198
Fragments arranged by size:
3198 1146 1071 738 252 105 85
BsmI GAATG_Cn'
Cuts at: 2351 3892 4106 4199 2351
Size: 1541 214 93 4747
Fragments arranged by size:
4747 1541 214 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 6595
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2739 3153 3405 3501 3510 3683
Size: 399 625 324 414 252 96 9 173
Cuts at: 3683 3914 4656 4741 5812 1391
Size: 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 414 399 324 252
231 173 96 85 9
BspEI T'CCGG_A
Cuts at: 6258 6258
Size: 6595
BspHI T'CATG_A
Cuts at: 4484 6214 4484
Size: 1730 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 3428 3950 844
Size: 2353 231 522 3489
Fragments arranged by size:
3489 2353 522 231
BsrBI CCG'CTC
Cuts at: 1394 1823 2398 2996 3394 4482 1394
Size: 429 575 598 398 1088 3507
Fragments arranged by size:
3507 1088 598 575 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 2505 2517 5090 5264 65
Size: 1183 1063 194 12 2573 174 1396
Fragments arranged by size:
2573 1396 1183 1063 194 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2724 5239 5426 1700
Size: 110 914 2515 187 2869
Fragments arranged by size:
2869 2515 914 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6595
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 4655 5667 4655
Size: 1012 5583
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6595
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 3091 3439 1184
Size: 1907 348 4340
Fragments arranged by size:
4340 1907 348
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2438 3375 2215
Size: 223 937 5435
Fragments arranged by size:
5435 937 223
BstYI r'GATC_y
Cuts at: 1972 2718 3000 3431 3618 3925 4292 4677 4694
Size: 746 282 431 187 307 367 385 17
Cuts at: 4694 6146 6232 6244 6590 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 746 431 385 367 346 307
282 187 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3408 3492 513
Size: 552 939 1404 84 3616
Fragments arranged by size:
3616 1404 939 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 3510 4111 4905 4925 3510
Size: 601 794 20 5180
Fragments arranged by size:
5180 794 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3253 3288 529
Size: 35 2689 35 3836
Fragments arranged by size:
3836 2689 35 35
DraI TTT'AAA
Cuts at: 3817 3899 3817
Size: 82 6513
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5602 809
Size: 507 4286 1802
Fragments arranged by size:
4286 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3195 3630
Size: 54 1425 50 275 18 546 819 435
Cuts at: 3630 4042 4931 6560 8
Size: 412 889 1629 43
Fragments arranged by size:
1629 1425 889 819 546 435 412 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4042 1537
Size: 275 2230 4090
Fragments arranged by size:
4090 2230 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3227 4525 1353
Size: 1586 288 1298 3423
Fragments arranged by size:
3423 1586 1298 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5182 5556 5702 387
Size: 1064 138 3593 374 146 1280
Fragments arranged by size:
3593 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2413
Size: 6595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 2723 2791 2934 2941 2987 3500
Size: 393 1077 14 68 143 7 46 513
Cuts at: 3500 3580 3634 3742 3789 4658 5255 6042 6310
Size: 80 54 108 47 869 597 787 268
Cuts at: 6310 1239
Size: 1524
Fragments arranged by size:
1524 1077 869 787 597 513 393 268
143 108 80 68 54 47 46 14
7
EcoO109I rG'GnC_Cy
Cuts at: 2743 3031 3209 3459 3497 2743
Size: 288 178 250 38 5841
Fragments arranged by size:
5841 288 250 178 38
EcoRI G'AATT_C
Cuts at: 2023 3904 4282 2023
Size: 1881 378 4336
Fragments arranged by size:
4336 1881 378
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2317 2555 3388 5742 1678
Size: 639 238 833 2354 2531
Fragments arranged by size:
2531 2354 833 639 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3123 3155 3423 3455 3787
Size: 32 135 32 1551 32 268 32 332
Cuts at: 3787 3819 5236 5268 5310 5342 1373
Size: 32 1417 32 42 32 2626
Fragments arranged by size:
2626 1551 1417 332 268 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3939 4185 669
Size: 988 281 2001 246 3079
Fragments arranged by size:
3079 2001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6595
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 2950 4194 2950
Size: 1244 5351
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5709 5893 1305
Size: 4404 184 2007
Fragments arranged by size:
4404 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 3197 3632 10
Size: 54 1425 343 546 819 435 2973
Fragments arranged by size:
2973 1425 819 546 435 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2372 2436 2540 3350 4553 4912
Size: 648 1047 159 64 104 810 1203 359
Cuts at: 4912 5071 518
Size: 159 2042
Fragments arranged by size:
2042 1203 1047 810 648 359 159 159
104 64
MspA1I CmG'CkG
Cuts at: 1606 2587 2601 2760 2767 4011 4688 5836 6081
Size: 981 14 159 7 1244 677 1148 245
Cuts at: 6081 1606
Size: 2120
Fragments arranged by size:
2120 1244 1148 981 677 245 159 14
7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5104
Fragments arranged by size:
5104 939 552
NdeI CA'TA_TG
Cuts at: 387 2327 387
Size: 1940 4655
NheI G'CTAG_C
Cuts at: 1052 3047 1052
Size: 1995 4600
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NsiI A_TGCA'T
Cuts at: 2447 2447
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 3256 3953 5498 1132
Size: 97 2027 697 1545 2229
Fragments arranged by size:
2229 2027 1545 697 97
PasI CC'CwG_GG
Cuts at: 1348 2105 1348
Size: 757 5838
PciI A'CATG_T
Cuts at: 5494 5494
Size: 6595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2162 2746 1108
Size: 1054 584 4957
Fragments arranged by size:
4957 1054 584
PfoI T'CCnGG_A
Cuts at: 2612 2739 2964 2612
Size: 127 225 6243
Fragments arranged by size:
6243 225 127
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4664 4696 6177 6209 4664
Size: 32 1481 32 5050
Fragments arranged by size:
5050 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 3031 3209 2743
Size: 288 178 6129
Fragments arranged by size:
6129 288 178
PshAI GACnn'nnGTC
Cuts at: 1613 3207 1613
Size: 1594 5001
PsiI TTA'TAA
Cuts at: 4165 4165
Size: 6595
PspOMI G'GGCC_C
Cuts at: 3497 3497
Size: 6595
PstI C_TGCA'G
Cuts at: 830 2718 3572 3947 830
Size: 1888 854 375 3478
Fragments arranged by size:
3478 1888 854 375
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
PvuII CAG'CTG
Cuts at: 2587 2601 2760 2767 2587
Size: 14 159 7 6415
Fragments arranged by size:
6415 159 14 7
SacI G_AGCT'C
Cuts at: 2415 2415
Size: 6595
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SbfI CC_TGCA'GG
Cuts at: 3947 3947
Size: 6595
ScaI AGT'ACT
Cuts at: 4843 6555 4843
Size: 1712 4883
SfcI C'TryA_G
Cuts at: 826 1045 2084 2714 3568 3943 5078 5759 5950
Size: 219 1039 630 854 375 1135 681 191
Cuts at: 5950 826
Size: 1471
Fragments arranged by size:
1471 1135 1039 854 681 630 375 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 3603 3922 1872
Size: 1731 319 4545
Fragments arranged by size:
4545 1731 319
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4699 5600 5862 820
Size: 197 926 2756 901 262 1553
Fragments arranged by size:
2756 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 3953 1132
Size: 2821 3774
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6595
StyI C'CwwG_G
Cuts at: 513 1065 2004 4034 513
Size: 552 939 2030 3074
Fragments arranged by size:
3074 2030 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2839 3616 4622 4639 4792 4977 5422
Size: 1255 1259 777 1006 17 153 185 445
Cuts at: 5422 325
Size: 1498
Fragments arranged by size:
1498 1259 1255 1006 777 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4841 6553 96
Size: 274 80 33 51 1443 2864 1712 138
Fragments arranged by size:
2864 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6555 6587 6555
Size: 32 6563
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6595
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 3601 3920 1870
Size: 1731 319 4545
Fragments arranged by size:
4545 1731 319
XmnI GAAnn'nnTTC
Cuts at: 4724 4724
Size: 6595
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4403 276
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI