Restriction map for
pFN21AE2549
(Circular) (Six-base) MAPSORT of: pFN21AE2549.seq Check: 671 from: 1 to: 9214
pFN21AE2549 9214 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4160 5155 5280 5542 6421 4160
Size: 995 125 262 879 6953
Fragments arranged by size:
6953 995 879 262 125
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2502 3090 4623 4806
Size: 53 83 186 936 966 588 1533 183
Cuts at: 4806 5631 7024 278
Size: 825 1393 2468
Fragments arranged by size:
2468 1533 1393 966 936 825 588 186
183 83 53
AccI GT'mk_AC
Cuts at: 1937 4917 6557 1937
Size: 2980 1640 4594
Fragments arranged by size:
4594 2980 1640
Acc65I G'GTAC_C
Cuts at: 6535 6535
Size: 9214
AclI AA'CG_TT
Cuts at: 1563 7341 7714 1563
Size: 5778 373 3063
Fragments arranged by size:
5778 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 2662 3432 3554 4001 4306 4579 5321
Size: 950 80 770 122 447 305 273 742
Cuts at: 5321 5408 5525 5596 6230 6278 7277 8661 8929
Size: 87 117 71 634 48 999 1384 268
Cuts at: 8929 1632
Size: 1917
Fragments arranged by size:
1917 1384 999 950 770 742 634 447
305 273 268 122 117 87 80 71
48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9017
AflIII A'CryG_T
Cuts at: 1203 2356 8113 9090 1203
Size: 1153 5757 977 1327
Fragments arranged by size:
5757 1327 1153 977
AgeI A'CCGG_T
Cuts at: 8045 8045
Size: 9214
AhdI GACnn_n'nnGTC
Cuts at: 7943 7943
Size: 9214
AleI CACnn'nnGTG
Cuts at: 1166 4124 1166
Size: 2958 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6017 1184
Size: 4833 4381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4400 4432 4886 4918 5789 5821 4400
Size: 32 454 32 871 32 7793
Fragments arranged by size:
7793 871 454 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3151 3250 3697 4114 6494 6957 8529 3151
Size: 99 447 417 2380 463 1572 3836
Fragments arranged by size:
3836 2380 1572 463 447 417 99
ApaI G_GGCC'C
Cuts at: 4770 6163 4770
Size: 1393 7821
ApaLI G'TGCA_C
Cuts at: 7271 8427 7271
Size: 1156 8058
ApoI r'AATT_y
Cuts at: 1493 6523 6749 6901 9010 1493
Size: 5030 226 152 2109 1697
Fragments arranged by size:
5030 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7768 160
Size: 872 6736 1606
Fragments arranged by size:
6736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9214
AvaI C'yCGr_G
Cuts at: 1870 1943 3004 3070 3635 4685 4951 5030 5435
Size: 73 1061 66 565 1050 266 79 405
Cuts at: 5435 5568 6177 6298 6409 6539 1870
Size: 133 609 121 111 130 4545
Fragments arranged by size:
4545 1061 1050 609 565 405 266 133
130 121 111 79 73 66
BamHI G'GATC_C
Cuts at: 3631 6216 6544 6911 3631
Size: 2585 328 367 5934
Fragments arranged by size:
5934 2585 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3044 3661 4349 5003 5733
Size: 325 217 627 1257 617 688 654 730
Cuts at: 5733 5788 6535 7990 618
Size: 55 747 1455 1842
Fragments arranged by size:
1842 1455 1257 747 730 688 654 627
617 325 217 55
BanII G_rGCy'C
Cuts at: 1391 2013 2712 3071 3934 4108 4770 5519 5575
Size: 622 699 359 863 174 662 749 56
Cuts at: 5575 6163 6176 6508 6533 1391
Size: 588 13 332 25 4072
Fragments arranged by size:
4072 863 749 699 662 622 588 359
332 174 56 25 13
BbeI G_GCGC'C
Cuts at: 3665 3665
Size: 9214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2389 3250 4063 4151 4390 4798 5309
Size: 971 490 861 813 88 239 408 511
Cuts at: 5309 5699 928
Size: 390 4443
Fragments arranged by size:
4443 971 861 813 511 490 408 390
239 88
BbvCI CC'TCA_GC
Cuts at: 2014 5264 6020 2014
Size: 3250 756 5208
Fragments arranged by size:
5208 3250 756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3074 3108 3184 3218 7405 7439 1903
Size: 34 1137 34 76 34 4187 34 3678
Fragments arranged by size:
4187 3678 1137 76 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7106 8316 7106
Size: 1210 8004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2757 3101
Size: 107 122 71 631 605 482 603 344
Cuts at: 3101 4172 4862 5001 7825 136
Size: 1071 690 139 2824 1525
Fragments arranged by size:
2824 1525 1071 690 631 605 603 482
344 139 122 107 71
BglII A'GATC_T
Cuts at: 3417 3451 5379 9209 3417
Size: 34 1928 3830 3422
Fragments arranged by size:
3830 3422 1928 34
BlpI GC'TnA_GC
Cuts at: 5673 6631 5673
Size: 958 8256
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2336 2741 3011 4352 4479 4770 5030
Size: 360 186 405 270 1341 127 291 260
Cuts at: 5030 5778 5793 6163 7275 8431 1790
Size: 748 15 370 1112 1156 2573
Fragments arranged by size:
2573 1341 1156 1112 748 405 370 360
291 270 260 186 127 15
BmgBI CAC'GTC
Cuts at: 1318 3289 3787 6367 1318
Size: 1971 498 2580 4165
Fragments arranged by size:
4165 2580 1971 498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 3367 6166
Size: 416 53 83 399 24 1080 870 2799
Cuts at: 6166 6945 7903 442
Size: 779 958 1753
Fragments arranged by size:
2799 1753 1080 958 870 779 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3830
Size: 9214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2440 2939 3014 3536 4190 5363 5387
Size: 916 285 499 75 522 654 1173 24
Cuts at: 5387 5564 5903 6464 7874 1239
Size: 177 339 561 1410 2579
Fragments arranged by size:
2579 1410 1173 916 654 561 522 499
339 285 177 75 24
Bpu10I CC'TnA_GC
Cuts at: 2014 2408 2684 5264 6020 2014
Size: 394 276 2580 756 5208
Fragments arranged by size:
5208 2580 756 394 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5315 7339 8204 8502 5315
Size: 2024 865 298 6027
Fragments arranged by size:
6027 2024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2098 3173 7877 882
Size: 617 599 1075 4704 2219
Fragments arranged by size:
4704 2219 1075 617 599
BsaAI yAC'GTr
Cuts at: 493 1204 3685 4450 5722 7005 493
Size: 711 2481 765 1272 1283 2702
Fragments arranged by size:
2702 2481 1283 1272 765 711
BsaBI GATnn'nnATC
Cuts at: 2975 9208 2975
Size: 6233 2981
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2499 2643 3087 3662
Size: 53 83 186 936 966 144 444 575
Cuts at: 3662 4620 4803 5472 5628 7021 7403 8038 275
Size: 958 183 669 156 1393 382 635 1451
Fragments arranged by size:
1451 1393 966 958 936 669 635 575
444 382 186 183 156 144 83 53
BsaWI w'CCGG_w
Cuts at: 3234 3816 4086 7647 8045 8319 8466 8877 3234
Size: 582 270 3561 398 274 147 411 3571
Fragments arranged by size:
3571 3561 582 411 398 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 4400 4430 5516
Size: 30 165 30 1035 30 1932 30 1086
Cuts at: 5516 5546 5789 5819 5876 5906 1178
Size: 30 243 30 57 30 4486
Fragments arranged by size:
4486 1932 1086 1035 243 165 57 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2318 2967 4013 4016 4037 4058 4292
Size: 874 258 649 1046 3 21 21 234
Cuts at: 4292 4544 5123 5234 5252 5369 5561 5675 5714
Size: 252 579 111 18 117 192 114 39
Cuts at: 5714 5729 6467 1186
Size: 15 738 3933
Fragments arranged by size:
3933 1046 874 738 649 579 258 252
234 192 117 114 111 39 21 21
18 15 3
BseYI C'CCAG_C
Cuts at: 2255 2481 3709 4762 8417 8913 2255
Size: 226 1228 1053 3655 496 2556
Fragments arranged by size:
3655 2556 1228 1053 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3440 3459 3548 3821 5189 5561 5981
Size: 573 1704 19 89 273 1368 372 420
Cuts at: 5981 6380 1163
Size: 399 3997
Fragments arranged by size:
3997 1704 1368 573 420 399 372 273
89 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 5233 5856 6664 7425 8453
Size: 275 186 1756 1476 623 808 761 1028
Cuts at: 8453 1540
Size: 2301
Fragments arranged by size:
2301 1756 1476 1028 808 761 623 275
186
BsiHKAI G_wGCw'C
Cuts at: 2712 4056 4455 4989 5178 6156 6234 7275 7360
Size: 1344 399 534 189 978 78 1041 85
Cuts at: 7360 8431 2712
Size: 1071 3495
Fragments arranged by size:
3495 1344 1071 1041 978 534 399 189
85 78
BsmI GAATG_Cn'
Cuts at: 2222 5731 6725 6818 2222
Size: 3509 994 93 4618
Fragments arranged by size:
4618 3509 994 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5792 6320 5792
Size: 528 8686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2150 2336 2712 2741 3011 3071
Size: 399 223 137 186 376 29 270 60
Cuts at: 3071 3934 4056 4108 4352 4455 4479 4770 4989
Size: 863 122 52 244 103 24 291 219
Cuts at: 4989 5030 5178 5519 5575 5778 5793 6156 6163
Size: 41 148 341 56 203 15 363 7
Cuts at: 6163 6176 6234 6508 6533 7275 7360 8431 1391
Size: 13 58 274 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 376 363 341
291 274 270 244 223 219 203 186
148 137 122 103 85 60 58 56
52 41 29 25 24 15 13 7
BspEI T'CCGG_A
Cuts at: 3234 8877 3234
Size: 5643 3571
BspHI T'CATG_A
Cuts at: 2475 2646 7103 8833 2475
Size: 171 4457 1730 2856
Fragments arranged by size:
4457 2856 1730 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 2837 3117 4160 4664 5109 5155 5280
Size: 1800 193 280 1043 504 445 46 125
Cuts at: 5280 5493 5542 6402 6421 6569 844
Size: 213 49 860 19 148 3489
Fragments arranged by size:
3489 1800 1043 860 504 445 280 213
193 148 125 49 46 19
BsrBI CCG'CTC
Cuts at: 1394 1823 7101 1394
Size: 429 5278 3507
Fragments arranged by size:
5278 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3090 3402 7709 7883 65
Size: 1183 1842 312 4307 174 1396
Fragments arranged by size:
4307 1842 1396 1183 312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2639 3756 4307 5056 7858 8045 1700
Size: 110 829 1117 551 749 2802 187 2869
Fragments arranged by size:
2869 2802 1117 829 749 551 187 110
BsrGI T'GTAC_A
Cuts at: 96 6276 96
Size: 6180 3034
BssHII G'CGCG_C
Cuts at: 1925 3651 1925
Size: 1726 7488
BssSI C'ACGA_G
Cuts at: 2861 3350 7274 8286 2861
Size: 489 3924 1012 3789
Fragments arranged by size:
3924 3789 1012 489
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3313 3619 794
Size: 2519 306 6389
Fragments arranged by size:
6389 2519 306
BstBI TT'CG_AA
Cuts at: 8018 8018
Size: 9214
BstEII G'GTnAC_C
Cuts at: 1184 2521 1184
Size: 1337 7877
BstYI r'GATC_y
Cuts at: 1972 2970 3219 3417 3451 3631 3975 4947 5379
Size: 998 249 198 34 180 344 972 432
Cuts at: 5379 6216 6544 6911 7296 7313 8765 8851 8863
Size: 837 328 367 385 17 1452 86 12
Cuts at: 8863 9209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 998 972 837 432 385 367
346 344 328 249 198 180 86 34
17 12
Bsu36I CC'TnA_GG
Cuts at: 2021 3838 5924 2021
Size: 1817 2086 5311
Fragments arranged by size:
5311 2086 1817
BtgI C'CryG_G
Cuts at: 513 1065 2004 2555 2772 2878 3657 3858 4248
Size: 552 939 551 217 106 779 201 390
Cuts at: 4248 4356 4389 5227 5475 5520 5655 5859 5868
Size: 108 33 838 248 45 135 204 9
Cuts at: 5868 5944 5985 6054 513
Size: 76 41 69 3673
Fragments arranged by size:
3673 939 838 779 552 551 390 248
217 204 201 135 108 106 76 69
45 41 33 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3934 5122 487
Size: 683 56 2708 1188 4579
Fragments arranged by size:
4579 2708 1188 683 56
BtsI GCAGTG_nn'
Cuts at: 3387 3418 3443 4987 6730 7524 7544 3387
Size: 31 25 1544 1743 794 20 5057
Fragments arranged by size:
5057 1743 1544 794 31 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9179
DraI TTT'AAA
Cuts at: 6518 6518
Size: 9214
DraIII CAC_nnn'GTG
Cuts at: 2340 3685 4080 6066 2340
Size: 1345 395 1986 5488
Fragments arranged by size:
5488 1986 1345 395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8221 809
Size: 507 6905 1802
Fragments arranged by size:
6905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 2881 2961
Size: 54 1425 50 275 18 982 69 80
Cuts at: 2961 3081 3102 3297 3346 4854 5230 5310 5853
Size: 120 21 195 49 1508 376 80 543
Cuts at: 5853 6236 6333 6661 7550 9179 8
Size: 383 97 328 889 1629 43
Fragments arranged by size:
1629 1508 1425 982 889 543 383 376
328 275 195 120 97 80 80 69
54 50 49 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 5230 5853 6661 1537
Size: 275 3418 623 808 4090
Fragments arranged by size:
4090 3418 808 623 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2558 3515 4092 4325 4517 4550 4643
Size: 776 429 957 577 233 192 33 93
Cuts at: 4643 4823 5111 5285 5501 6095 7144 1353
Size: 180 288 174 216 594 1049 3423
Fragments arranged by size:
3423 1049 957 776 594 577 429 288
233 216 192 180 174 93 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4055 5639 6088 7801 8175 8321
Size: 1064 138 2466 1584 449 1713 374 146
Cuts at: 8321 387
Size: 1280
Fragments arranged by size:
2466 1713 1584 1280 1064 449 374 146
138
EcoICRI GAG'CTC
Cuts at: 2710 2710
Size: 9214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2440 2582 2662 2939 3014 3432
Size: 393 523 285 142 80 277 75 418
Cuts at: 3432 3536 3554 4001 4190 4306 4579 5321 5363
Size: 104 18 447 189 116 273 742 42
Cuts at: 5363 5387 5408 5525 5564 5596 5903 6230 6278
Size: 24 21 117 39 32 307 327 48
Cuts at: 6278 6464 7277 7874 8661 8929 1239
Size: 186 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 742 597 523 447 418
393 327 307 285 277 273 268 189
186 142 117 116 104 80 75 48
42 39 32 24 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2588 4223 5216 6224 6482 2588
Size: 1635 993 1008 258 5320
Fragments arranged by size:
5320 1635 1008 993 258
EcoO109I rG'GnC_Cy
Cuts at: 2344 2610 2631 2758 2874 4766 6160 6414 2344
Size: 266 21 127 116 1892 1394 254 5144
Fragments arranged by size:
5144 1892 1394 266 254 127 116 21
EcoRI G'AATT_C
Cuts at: 6523 6901 6523
Size: 378 8836
EcoRV GAT'ATC
Cuts at: 3229 3229
Size: 9214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4603 4822 4603
Size: 219 8995
FseI GG_CCGG'CC
Cuts at: 5060 5060
Size: 9214
FspI TGC'GCA
Cuts at: 7720 7720
Size: 9214
HaeII r_GCGC'y
Cuts at: 1678 3643 3665 4037 4888 5206 8361 1678
Size: 1965 22 372 851 318 3155 2531
Fragments arranged by size:
3155 2531 1965 851 372 318 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 2571 2603 4899
Size: 32 135 32 866 32 101 32 2296
Cuts at: 4899 4931 5876 5908 6184 6216 7855 7887 7929
Size: 32 945 32 276 32 1639 32 42
Cuts at: 7929 7961 1373
Size: 32 2626
Fragments arranged by size:
2626 2296 1639 945 866 276 135 101
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5470 6558 6804 669
Size: 988 281 3532 1088 246 3079
Fragments arranged by size:
3532 3079 1088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9214
HpaI GTT'AAC
Cuts at: 6804 6804
Size: 9214
KasI G'GCGC_C
Cuts at: 3661 3661
Size: 9214
KpnI G_GTAC'C
Cuts at: 6539 6539
Size: 9214
MfeI C'AATT_G
Cuts at: 6813 6813
Size: 9214
MluI A'CGCG_T
Cuts at: 9090 9090
Size: 9214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3510 8328 8512 1305
Size: 2205 4818 184 2007
Fragments arranged by size:
4818 2205 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2814 2963 3104 3299 3348
Size: 54 1425 343 982 149 141 195 49
Cuts at: 3348 4856 10
Size: 1508 4368
Fragments arranged by size:
4368 1508 1425 982 343 195 149 141
54 49
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 4124 4781 5141 5724 6272 7172
Size: 648 1695 1263 657 360 583 548 900
Cuts at: 7172 7531 7690 518
Size: 359 159 2042
Fragments arranged by size:
2042 1695 1263 900 657 648 583 548
360 359 159
MspA1I CmG'CkG
Cuts at: 1606 2557 3247 3370 3473 3532 3659 3956 4297
Size: 951 690 123 103 59 127 297 341
Cuts at: 4297 4358 5431 5607 5657 6024 6494 6630 7307
Size: 61 1073 176 50 367 470 136 677
Cuts at: 7307 8455 8700 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1073 951 690 677 470 367
341 297 245 176 136 127 123 103
61 59 50
NaeI GCC'GGC
Cuts at: 5058 5058
Size: 9214
NarI GG'CG_CC
Cuts at: 3662 3662
Size: 9214
NcoI C'CATG_G
Cuts at: 513 1065 2004 3858 5475 5520 5859 5868 5985
Size: 552 939 1854 1617 45 339 9 117
Cuts at: 5985 6054 513
Size: 69 3673
Fragments arranged by size:
3673 1854 1617 939 552 339 117 69
45 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9214
NgoMIV G'CCGG_C
Cuts at: 5056 5056
Size: 9214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9214
NotI GC'GGCC_GC
Cuts at: 5853 6661 5853
Size: 808 8406
NspI r_CATG'y
Cuts at: 1132 1229 2360 4285 6572 8117 1132
Size: 97 1131 1925 2287 1545 2229
Fragments arranged by size:
2287 2229 1925 1545 1131 97
PasI CC'CwG_GG
Cuts at: 1348 5793 1348
Size: 4445 4769
PciI A'CATG_T
Cuts at: 2356 8113 2356
Size: 5757 3457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4230 6426 1108
Size: 3122 2196 3896
Fragments arranged by size:
3896 3122 2196
PfoI T'CCnGG_A
Cuts at: 2361 3165 3261 3513 6351 2361
Size: 804 96 252 2838 5224
Fragments arranged by size:
5224 2838 804 252 96
PmeI GTTT'AAAC
Cuts at: 6518 6518
Size: 9214
PmlI CAC'GTG
Cuts at: 3685 5722 3685
Size: 2037 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5789 5821 7283 7315 8796 8828 4400
Size: 32 1357 32 1462 32 1481 32 4786
Fragments arranged by size:
4786 1481 1462 1357 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2344 2631 2874 2344
Size: 287 243 8684
Fragments arranged by size:
8684 287 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9214
PsiI TTA'TAA
Cuts at: 6784 6784
Size: 9214
PspOMI G'GGCC_C
Cuts at: 4766 6159 4766
Size: 1393 7821
PstI C_TGCA'G
Cuts at: 830 3615 3675 3971 4161 4341 4661 4994 5277
Size: 2785 60 296 190 180 320 333 283
Cuts at: 5277 6098 6566 830
Size: 821 468 3478
Fragments arranged by size:
3478 2785 821 468 333 320 296 283
190 180 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9214
PvuII CAG'CTG
Cuts at: 3247 3370 3473 3532 6024 3247
Size: 123 103 59 2492 6437
Fragments arranged by size:
6437 2492 123 103 59
SacI G_AGCT'C
Cuts at: 2712 2712
Size: 9214
SacII CC_GC'GG
Cuts at: 2558 3660 4359 5658 2558
Size: 1102 699 1299 6114
Fragments arranged by size:
6114 1299 1102 699
SalI G'TCGA_C
Cuts at: 1936 6556 1936
Size: 4620 4594
SanDI GG'GwC_CC
Cuts at: 2344 2631 2874 2344
Size: 287 243 8684
Fragments arranged by size:
8684 287 243
SapI GCTCTTCn'nnn_
Cuts at: 2558 3515 4517 4550 4643 5111 5285 6095 2558
Size: 957 1002 33 93 468 174 810 5677
Fragments arranged by size:
5677 1002 957 810 468 174 93 33
SbfI CC_TGCA'GG
Cuts at: 4661 6566 4661
Size: 1905 7309
ScaI AGT'ACT
Cuts at: 7462 9174 7462
Size: 1712 7502
SfcI C'TryA_G
Cuts at: 826 1045 2141 3611 3671 3967 4157 4337 4657
Size: 219 1096 1470 60 296 190 180 320
Cuts at: 4657 4990 5273 6094 6562 7697 8378 8569 826
Size: 333 283 821 468 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 1096 821 681 468 333
320 296 283 219 191 190 180 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 4862 4862
Size: 9214
SfoI GGC'GCC
Cuts at: 3663 3663
Size: 9214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9214
SgrAI Cr'CCGG_yG
Cuts at: 1700 2639 1700
Size: 939 8275
SmaI CCC'GGG
Cuts at: 1872 3006 4687 4953 6179 6541 1872
Size: 1134 1681 266 1226 362 4545
Fragments arranged by size:
4545 1681 1226 1134 362 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5330 7318 8219 8481 820
Size: 197 926 3387 1988 901 262 1553
Fragments arranged by size:
3387 1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7005 493
Size: 6512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9214
SphI G_CATG'C
Cuts at: 1132 4285 6572 1132
Size: 3153 2287 3774
Fragments arranged by size:
3774 3153 2287
SrfI GCCC'GGGC
Cuts at: 3006 3006
Size: 9214
SspI AAT'ATT
Cuts at: 5 52 7138 5
Size: 47 7086 2081
Fragments arranged by size:
7086 2081 47
StuI AGG'CCT
Cuts at: 1517 2583 3311 4136 4214 1517
Size: 1066 728 825 78 6517
Fragments arranged by size:
6517 1066 825 728 78
StyI C'CwwG_G
Cuts at: 513 1065 2004 2815 2943 3858 3960 3993 4176
Size: 552 939 811 128 915 102 33 183
Cuts at: 4176 4224 4518 5475 5520 5859 5868 5985 6054
Size: 48 294 957 45 339 9 117 69
Cuts at: 6054 6653 513
Size: 599 3074
Fragments arranged by size:
3074 957 939 915 811 599 552 339
294 183 128 117 102 69 48 45
33 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4825 7241 7258 7411 7596 8041 325
Size: 1255 3245 2416 17 153 185 445 1498
Fragments arranged by size:
3245 2416 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 4590 5904
Size: 274 80 33 51 1443 816 1797 1314
Cuts at: 5904 6276 7460 9172 96
Size: 372 1184 1712 138
Fragments arranged by size:
1797 1712 1443 1314 1184 816 372 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9174 9206 9174
Size: 32 9182
Tth111I GACn'n_nGTC
Cuts at: 1316 2393 1316
Size: 1077 8137
XbaI T'CTAG_A
Cuts at: 6550 6550
Size: 9214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 3341 5866 2983
Size: 358 2525 6331
Fragments arranged by size:
6331 2525 358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9214
XmaI C'CCGG_G
Cuts at: 1870 3004 4685 4951 6177 6539 1870
Size: 1134 1681 266 1226 362 4545
Fragments arranged by size:
4545 1681 1226 1134 362 266
XmnI GAAnn'nnTTC
Cuts at: 3509 7343 3509
Size: 3834 5380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2500 3088 4621 4804
Size: 53 83 186 936 966 588 1533 183
Cuts at: 4804 5629 7022 276
Size: 825 1393 2468
Fragments arranged by size:
2468 1533 1393 966 936 825 588 186
183 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI
SwaI