Restriction map for
pFN21AE2545
(Circular) (Six-base) MAPSORT of: pFN21AE2545.seq Check: 1096 from: 1 to: 5311
pFN21AE2545 5311 bp
With 182 enzymes: SgfI *
September 30, 2014 11:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2304 3121 278
Size: 53 83 186 936 768 817 2468
Fragments arranged by size:
2468 936 817 768 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3374 4758 5026 1632
Size: 947 795 1384 268 1917
Fragments arranged by size:
1917 1384 947 795 268
AfeI AGC'GCT
Cuts at: 2564 2564
Size: 5311
AflII C'TTAA_G
Cuts at: 820 1017 2312 820
Size: 197 1295 3819
Fragments arranged by size:
3819 1295 197
AflIII A'CryG_T
Cuts at: 1203 2124 4210 5187 1203
Size: 921 2086 977 1327
Fragments arranged by size:
2086 1327 977 921
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2261 2620 2846 2998 5107 1493
Size: 768 359 226 152 2109 1697
Fragments arranged by size:
2109 1697 768 359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2636 1870
Size: 73 262 431 4545
Fragments arranged by size:
4545 431 262 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2632 4087 618
Size: 325 217 627 759 86 1455 1842
Fragments arranged by size:
1842 1455 759 627 325 217 86
BanII G_rGCy'C
Cuts at: 1391 2372 2445 2630 1391
Size: 981 73 185 4072
Fragments arranged by size:
4072 981 185 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 3695
Fragments arranged by size:
3695 971 645
BbvCI CC'TCA_GC
Cuts at: 2236 2236
Size: 5311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3922 136
Size: 107 122 71 631 605 2250 1525
Fragments arranged by size:
2250 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2551 3372 4528 1790
Size: 761 821 1156 2573
Fragments arranged by size:
2573 1156 821 761
BmgBI CAC'GTC
Cuts at: 1318 2127 1318
Size: 809 4502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2557 2461
Size: 96 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 3971 1239
Size: 1217 1515 2579
Fragments arranged by size:
2579 1515 1217
Bpu10I CC'TnA_GC
Cuts at: 2236 2366 2236
Size: 130 5181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 3436 4301 4599 2320
Size: 1116 865 298 3032
Fragments arranged by size:
3032 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 3974 882
Size: 617 525 1950 2219
Fragments arranged by size:
2219 1950 617 525
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 2600 3118 3500
Size: 53 83 186 936 768 299 518 382
Cuts at: 3500 4135 275
Size: 635 1451
Fragments arranged by size:
1451 936 768 635 518 382 299 186
83 53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 4369
Fragments arranged by size:
4369 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2446 2453 1186
Size: 1260 7 4044
Fragments arranged by size:
4044 1260 7
BseYI C'CCAG_C
Cuts at: 2342 2349 2371 2406 2419 4514 5010 2342
Size: 7 22 35 13 2095 496 2643
Fragments arranged by size:
2643 2095 496 35 22 13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2761 3522 4550 1540
Size: 275 186 17 743 761 1028 2301
Fragments arranged by size:
2301 1028 761 743 275 186 17
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2372 2445 2551 2630 3372 3457 4528
Size: 399 582 73 106 79 742 85 1071
Cuts at: 4528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 582 399 106 85 79
73
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 844
Size: 1822 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 3955 4142 1700
Size: 110 203 1942 187 2869
Fragments arranged by size:
2869 1942 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2430 2641 3008 3393 3410 4862 4948 4960
Size: 458 211 367 385 17 1452 86 12
Cuts at: 4960 5306 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 458 385 367 346 211 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2132 2132
Size: 5311
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4759
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2758 3647
Size: 54 1425 50 275 18 243 685 889
Cuts at: 3647 5276 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 685 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 3241 1353
Size: 1173 715 3423
Fragments arranged by size:
3423 1173 715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3898 4272 4418 387
Size: 1064 138 453 1856 374 146 1280
Fragments arranged by size:
1856 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2579 3374 3971 4758 5026 1239
Size: 393 824 123 795 597 787 268 1524
Fragments arranged by size:
1524 824 795 787 597 393 268 123
EcoNI CCTnn'n_nnAGG
Cuts at: 2489 2489
Size: 5311
EcoRI G'AATT_C
Cuts at: 2261 2620 2998 2261
Size: 359 378 4574
Fragments arranged by size:
4574 378 359
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2566 4458 1678
Size: 888 1892 2531
Fragments arranged by size:
2531 1892 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 4425 4609 1305
Size: 1104 2016 184 2007
Fragments arranged by size:
2016 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3628 3787 518
Size: 648 2103 359 159 2042
Fragments arranged by size:
2103 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2727 3404 4552 4797 1606
Size: 1121 677 1148 245 2120
Fragments arranged by size:
2120 1148 1121 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PfoI T'CCnGG_A
Cuts at: 2265 2433 2265
Size: 168 5143
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2519 1613
Size: 906 4405
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
RsrII CG'GwC_CG
Cuts at: 2020 2020
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2659 3794 4475 4666 826
Size: 219 1614 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2335 3415 4316 4578 820
Size: 197 926 369 23 1080 901 262 1553
Fragments arranged by size:
1553 1080 926 901 369 262 197 23
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2046 2490 2750 513
Size: 552 981 444 260 3074
Fragments arranged by size:
3074 981 552 444 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2098 2108 3338 3355 3508 3693
Size: 1255 428 90 10 1230 17 153 185
Cuts at: 3693 4138 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1230 445 428 185 153 90
17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 5271 5303 2090
Size: 32 3149 32 2098
Fragments arranged by size:
3149 2098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2599 1316
Size: 1283 4028
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 2502 2564 3440 2502
Size: 62 876 4373
Fragments arranged by size:
4373 876 62
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2302 3119 276
Size: 53 83 186 936 768 817 2468
Fragments arranged by size:
2468 936 817 768 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI