Restriction map for
pFN21AE2541
(Circular) (Six-base) MAPSORT of: pFN21AE2541.seq Check: 6048 from: 1 to: 5674
pFN21AE2541 5674 bp
With 182 enzymes: SgfI *
August 29, 2014 11:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 3017 1937
Size: 271 809 4594
Fragments arranged by size:
4594 809 271
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2785 2952 3737 5121 5389 1632
Size: 1153 167 785 1384 268 1917
Fragments arranged by size:
1917 1384 1153 785 268 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 2203 4505 2203
Size: 2302 3372
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2386 1184
Size: 1202 4472
AlwNI CAG_nnn'CTG
Cuts at: 2306 2374 3417 4989 2306
Size: 68 1043 1572 2991
Fragments arranged by size:
2991 1572 1043 68
ApaI G_GGCC'C
Cuts at: 2715 2715
Size: 5674
ApaLI G'TGCA_C
Cuts at: 2320 3731 4887 2320
Size: 1411 1156 3107
Fragments arranged by size:
3107 1411 1156
ApoI r'AATT_y
Cuts at: 1493 2126 2774 2983 3209 3361 5470 1493
Size: 633 648 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 648 633 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BamHI G'GATC_C
Cuts at: 2165 3004 3371 2165
Size: 839 367 4468
Fragments arranged by size:
4468 839 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 2995 4450 618
Size: 325 217 627 843 365 1455 1842
Fragments arranged by size:
1842 1455 843 627 365 325 217
BanII G_rGCy'C
Cuts at: 1391 2715 2993 1391
Size: 1324 278 4072
Fragments arranged by size:
4072 1324 278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2749 2958 928
Size: 971 445 405 209 3644
Fragments arranged by size:
3644 971 445 405 209
BbvCI CC'TCA_GC
Cuts at: 2156 2156
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2324 2715 3735 4891 1790
Size: 534 391 1020 1156 2573
Fragments arranged by size:
2573 1156 1020 534 391
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2188 2362 2415
Size: 416 53 83 399 24 771 174 53
Cuts at: 2415 3405 4363 442
Size: 990 958 1753
Fragments arranged by size:
1753 990 958 771 416 399 174 83
53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2747
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2913 2973 4334 1239
Size: 1674 60 1361 2579
Fragments arranged by size:
2579 1674 1361 60
Bpu10I CC'TnA_GC
Cuts at: 2156 2156
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2082 3799 4664 4962 2082
Size: 1717 865 298 2794
Fragments arranged by size:
2794 1717 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2486 4337 882
Size: 617 987 1851 2219
Fragments arranged by size:
2219 1851 987 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 2336 5668 2336
Size: 3332 2342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2203 4107 4505 4779 4926 5337 2203
Size: 1904 398 274 147 411 2540
Fragments arranged by size:
2540 1904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2942 1186
Size: 958 798 3918
Fragments arranged by size:
3918 958 798
BseYI C'CCAG_C
Cuts at: 2814 4877 5373 2814
Size: 2063 496 3115
Fragments arranged by size:
3115 2063 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 1163
Size: 573 543 4558
Fragments arranged by size:
4558 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2208 3124 3885 4913 1540
Size: 275 186 207 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 2324 3735 3820 4891 2324
Size: 1411 85 1071 3107
Fragments arranged by size:
3107 1411 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
BsmBI CGTCTCn'nnnn_
Cuts at: 2861 2861
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 2715 2993 3735 3820 4891 1391
Size: 399 534 391 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 399 391 278 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 3029 844
Size: 1827 358 3489
Fragments arranged by size:
3489 1827 358
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2244 4169 4343 65
Size: 1183 996 1925 174 1396
Fragments arranged by size:
1925 1396 1183 996 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2203 4318 4505 1700
Size: 110 393 2115 187 2869
Fragments arranged by size:
2869 2115 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2428
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2165 3004 3371 3756 3773 5225 5311 5323
Size: 193 839 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 839 385 367 346 193 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 2313 2448 513
Size: 552 1188 60 135 3739
Fragments arranged by size:
3739 1188 552 135 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2323 2344 487
Size: 683 56 1097 21 3817
Fragments arranged by size:
3817 1097 683 56 21
BtsI GCAGTG_nn'
Cuts at: 2267 2307 3190 3984 4004 2267
Size: 40 883 794 20 3937
Fragments arranged by size:
3937 883 794 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2164 2199 529
Size: 35 1600 35 4004
Fragments arranged by size:
4004 1600 35 35
DraI TTT'AAA
Cuts at: 2791 2978 2791
Size: 187 5487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2269 2284
Size: 54 1425 50 275 18 303 136 15
Cuts at: 2284 2310 3121 4010 5639 8
Size: 26 811 889 1629 43
Fragments arranged by size:
1629 1425 889 811 303 275 136 54
50 43 26 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2785 2913 2952 2973 3737 4334 5121
Size: 393 1153 128 39 21 764 597 787
Cuts at: 5121 5389 1239
Size: 268 1524
Fragments arranged by size:
1524 1153 787 764 597 393 268 128
39 21
EcoRI G'AATT_C
Cuts at: 2774 2983 3361 2774
Size: 209 378 5087
Fragments arranged by size:
5087 378 209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5674
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2052 2084 2157 2189 2382
Size: 32 135 32 480 32 73 32 193
Cuts at: 2382 2414 2948 2980 4315 4347 4389 4421 1373
Size: 32 534 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 534 480 193 135 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2917 3018 3264 669
Size: 988 281 271 708 101 246 3079
Fragments arranged by size:
3079 988 708 281 271 246 101
HindIII A'AGCT_T
Cuts at: 748 2100 748
Size: 1352 4322
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 2510 2580 2598 3273 2510
Size: 70 18 675 4911
Fragments arranged by size:
4911 675 70 18
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2271 2286 2312 10
Size: 54 1425 343 303 136 15 26 3372
Fragments arranged by size:
3372 1425 343 303 136 54 26 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2840 3632 3991 4150 518
Size: 648 906 768 792 359 159 2042
Fragments arranged by size:
2042 906 792 768 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2123 2280 2471 2535 3090 3767 4915 5160
Size: 517 157 191 64 555 677 1148 245
Cuts at: 5160 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 555 517 245 191 157
64
NcoI C'CATG_G
Cuts at: 513 1065 2253 2313 2448 513
Size: 552 1188 60 135 3739
Fragments arranged by size:
3739 1188 552 135 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2906 3032 4577 1132
Size: 97 1677 126 1545 2229
Fragments arranged by size:
2229 1677 1545 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2178 2374 1108
Size: 1070 196 4408
Fragments arranged by size:
4408 1070 196
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2555 1613
Size: 942 4732
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2711 2711
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2302 2668 3026 830
Size: 1472 366 358 3478
Fragments arranged by size:
3478 1472 366 358
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2535 2535
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 2207 3016 1936
Size: 271 809 4594
Fragments arranged by size:
4594 809 271
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2298 2664 3022 4157 4838 5029 826
Size: 219 1253 366 358 1135 681 191 1471
Fragments arranged by size:
1471 1253 1135 681 366 358 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2097 3778 4679 4941 820
Size: 197 926 154 1681 901 262 1553
Fragments arranged by size:
1681 1553 926 901 262 197 154
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 2436 1517
Size: 919 4755
StyI C'CwwG_G
Cuts at: 513 1065 2253 2313 2448 3113 513
Size: 552 1188 60 135 665 3074
Fragments arranged by size:
3074 1188 665 552 135 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3718 3871 4056 4501 325
Size: 1255 2121 17 153 185 445 1498
Fragments arranged by size:
2121 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI