Restriction map for
pFN21AE2533
(Circular) (Six-base) MAPSORT of: pFN21AE2533.seq Check: 3953 from: 1 to: 6283
pFN21AE2533 6283 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2809 2809
Size: 6283
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3626 1937
Size: 487 1202 4594
Fragments arranged by size:
4594 1202 487
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 3510 4346 5730 5998 1632
Size: 1089 789 836 1384 268 1917
Fragments arranged by size:
1917 1384 1089 836 789 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 5012 5012
Size: 6283
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 2921 4026 5598 2921
Size: 1105 1572 3606
Fragments arranged by size:
3606 1572 1105
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 6283
ApaLI G'TGCA_C
Cuts at: 4340 5496 4340
Size: 1156 5127
ApoI r'AATT_y
Cuts at: 1493 3592 3818 3970 6079 1493
Size: 2099 226 152 2109 1697
Fragments arranged by size:
2109 2099 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 3608 1870
Size: 73 1665 4545
Fragments arranged by size:
4545 1665 73
BamHI G'GATC_C
Cuts at: 2888 3613 3980 2888
Size: 725 367 5191
Fragments arranged by size:
5191 725 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 3604 5059 618
Size: 325 217 627 223 1594 1455 1842
Fragments arranged by size:
1842 1594 1455 627 325 223 217
BanII G_rGCy'C
Cuts at: 1391 2283 2856 3602 1391
Size: 892 573 746 4072
Fragments arranged by size:
4072 892 746 573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3190 3377 928
Size: 971 1291 187 3834
Fragments arranged by size:
3834 1291 971 187
BbvCI CC'TCA_GC
Cuts at: 2768 3146 2768
Size: 378 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4175 5385 4175
Size: 1210 5073
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4894 136
Size: 107 122 71 631 605 3222 1525
Fragments arranged by size:
3222 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2386 6278 2386
Size: 3892 2391
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2283 4344 5500 1790
Size: 223 270 2061 1156 2573
Fragments arranged by size:
2573 2061 1156 270 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 2681 4014
Size: 416 53 83 399 24 1240 24 1333
Cuts at: 4014 4972 442
Size: 958 1753
Fragments arranged by size:
1753 1333 1240 958 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2437 4943 1239
Size: 1198 2506 2579
Fragments arranged by size:
2579 2506 1198
Bpu10I CC'TnA_GC
Cuts at: 2768 3146 2768
Size: 378 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4408 5273 5571 4408
Size: 865 298 5120
Fragments arranged by size:
5120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4946 882
Size: 617 3447 2219
Fragments arranged by size:
3447 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4716 5114 5388 5535 5946 4716
Size: 398 274 147 411 5053
Fragments arranged by size:
5053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6058
Fragments arranged by size:
6058 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6283
BseYI C'CCAG_C
Cuts at: 2106 2181 3176 3504 5486 5982 2106
Size: 75 995 328 1982 496 2407
Fragments arranged by size:
2407 1982 995 496 328 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 1163
Size: 573 1051 4659
Fragments arranged by size:
4659 1051 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2296 3733 4494 5522 1540
Size: 275 186 295 1437 761 1028 2301
Fragments arranged by size:
2301 1437 1028 761 295 275 186
BsiHKAI G_wGCw'C
Cuts at: 2856 3411 4344 4429 5500 2856
Size: 555 933 85 1071 3639
Fragments arranged by size:
3639 1071 933 555 85
BsmI GAATG_Cn'
Cuts at: 3794 3887 3794
Size: 93 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2283 2856 3411 3602 4344 4429
Size: 399 223 270 573 555 191 742 85
Cuts at: 4429 5500 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 573 555 399 270 223
191 85
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 4172 5902 4172
Size: 1730 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 2906 3053 3571 3638 844
Size: 1965 97 147 518 67 3489
Fragments arranged by size:
3489 1965 518 147 97 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4170 1394
Size: 429 2347 3507
Fragments arranged by size:
3507 2347 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3291 4778 4952 65
Size: 1183 2043 1487 174 1396
Fragments arranged by size:
2043 1487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3374 4927 5114 1700
Size: 110 1564 1553 187 2869
Fragments arranged by size:
2869 1564 1553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6283
BstXI CCAn_nnnn'nTGG
Cuts at: 2473 2473
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2386 2888 3613 3980 4365 4382 5834 5920
Size: 414 502 725 367 385 17 1452 86
Cuts at: 5920 5932 6278 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 725 502 414 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2425 2425
Size: 6283
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2686 3799 4593 4613 2100
Size: 586 1113 794 20 3770
Fragments arranged by size:
3770 1113 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DraIII CAC_nnn'GTG
Cuts at: 2035 2035
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248
Size: 54 1425 50 275 18 1900 889 1629
Cuts at: 6248 8
Size: 43
Fragments arranged by size:
1900 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4213 1353
Size: 2860 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 6283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 2721 3510 4346 4943 5730 5998
Size: 393 805 284 789 836 597 787 268
Cuts at: 5998 1239
Size: 1524
Fragments arranged by size:
1524 836 805 789 787 597 393 284
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3112 3112
Size: 6283
EcoO109I rG'GnC_Cy
Cuts at: 2279 2598 2636 2279
Size: 319 38 5926
Fragments arranged by size:
5926 319 38
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 2590 2622 3014
Size: 32 135 32 961 32 25 32 392
Cuts at: 3014 3046 4924 4956 4998 5030 1373
Size: 32 1878 32 42 32 2626
Fragments arranged by size:
2626 1878 961 392 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3627 3873 669
Size: 988 281 1689 246 3079
Fragments arranged by size:
3079 1689 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 3450 5397 5581 1305
Size: 1185 960 1947 184 2007
Fragments arranged by size:
2007 1947 1185 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4461
Fragments arranged by size:
4461 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 3447 4241 4600 4759 518
Size: 648 1707 574 794 359 159 2042
Fragments arranged by size:
2042 1707 794 648 574 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2292 2581 3699 4376 5524 5769 1606
Size: 596 90 289 1118 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 596 289 245 90
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 3354 3641 5186 1132
Size: 97 2125 287 1545 2229
Fragments arranged by size:
2229 2125 1545 287 97
PasI CC'CwG_GG
Cuts at: 1348 2276 3130 1348
Size: 928 854 4501
Fragments arranged by size:
4501 928 854
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3078 1108
Size: 1970 4313
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2598 2636 2598
Size: 38 6245
PshAI GACnn'nnGTC
Cuts at: 1613 2939 3011 1613
Size: 1326 72 4885
Fragments arranged by size:
4885 1326 72
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 6283
PstI C_TGCA'G
Cuts at: 830 2580 3635 830
Size: 1750 1055 3478
Fragments arranged by size:
3478 1750 1055
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 2202 2581 2202
Size: 379 5904
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 6283
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 2810 4531 6243 2810
Size: 1721 1712 2850
Fragments arranged by size:
2850 1721 1712
SfcI C'TryA_G
Cuts at: 826 1045 2197 2576 3631 4766 5447 5638 826
Size: 219 1152 379 1055 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 1055 681 379 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3610 1872
Size: 1738 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4387 5288 5550 820
Size: 197 926 2444 901 262 1553
Fragments arranged by size:
2444 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6283
StyI C'CwwG_G
Cuts at: 513 1065 2004 3722 513
Size: 552 939 1718 3074
Fragments arranged by size:
3074 1718 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 4310 4327 4480 4665 5110 325
Size: 1255 777 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2808 4529 6241
Size: 274 80 33 51 1443 831 1721 1712
Cuts at: 6241 96
Size: 138
Fragments arranged by size:
1721 1712 1443 831 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 4912
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 3608 1870
Size: 1738 4545
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI