Restriction map for
pFN21AE2529
(Circular) (Six-base) MAPSORT of: pFN21AE2529.seq Check: 8301 from: 1 to: 5281
pFN21AE2529 5281 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3091 278
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3344 4728 4996 1632
Size: 1712 1384 268 1917
Fragments arranged by size:
1917 1712 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 4180 5157 1203
Size: 2977 977 1327
Fragments arranged by size:
2977 1327 977
AgeI A'CCGG_T
Cuts at: 4112 4112
Size: 5281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5281
AlwNI CAG_nnn'CTG
Cuts at: 2269 3024 4596 2269
Size: 755 1572 2954
Fragments arranged by size:
2954 1572 755
ApaLI G'TGCA_C
Cuts at: 3338 4494 3338
Size: 1156 4125
ApoI r'AATT_y
Cuts at: 1493 2590 2816 2968 5077 1493
Size: 1097 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2330 3835 160
Size: 872 1298 1505 1606
Fragments arranged by size:
1606 1505 1298 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2606 1870
Size: 73 109 554 4545
Fragments arranged by size:
4545 554 109 73
BamHI G'GATC_C
Cuts at: 2611 2978 2611
Size: 367 4914
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2084 2602 4057 618
Size: 325 217 627 261 36 518 1455 1842
Fragments arranged by size:
1842 1455 627 518 325 261 217 36
BanII G_rGCy'C
Cuts at: 1391 2600 1391
Size: 1209 4072
BbeI G_GCGC'C
Cuts at: 2052 2088 2052
Size: 36 5245
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3472 3506 1903
Size: 34 1535 34 3678
Fragments arranged by size:
3678 1535 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 4383 3173
Size: 1210 4071
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3892 136
Size: 107 122 71 631 605 2220 1525
Fragments arranged by size:
2220 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2698 2698
Size: 5281
Bme1580I G_kGCm'C
Cuts at: 1790 2523 3342 4498 1790
Size: 733 819 1156 2573
Fragments arranged by size:
2573 1156 819 733
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3970 442
Size: 416 53 83 399 24 1595 958 1753
Fragments arranged by size:
1753 1595 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2550 3941 1239
Size: 1311 1391 2579
Fragments arranged by size:
2579 1391 1311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3406 4271 4569 3406
Size: 865 298 4118
Fragments arranged by size:
4118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3944 882
Size: 617 2445 2219
Fragments arranged by size:
2445 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3072 493
Size: 711 1868 2702
Fragments arranged by size:
2702 1868 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2085 2145 3088
Size: 53 83 186 936 516 36 60 943
Cuts at: 3088 3470 4105 275
Size: 382 635 1451
Fragments arranged by size:
1451 943 936 635 516 382 186 83
60 53 36
BsaWI w'CCGG_w
Cuts at: 3714 4112 4386 4533 4944 3714
Size: 398 274 147 411 4051
Fragments arranged by size:
4051 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5056
Fragments arranged by size:
5056 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 1186
Size: 1069 4212
BseYI C'CCAG_C
Cuts at: 2026 2195 4484 4980 2026
Size: 169 2289 496 2327
Fragments arranged by size:
2327 2289 496 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 1163
Size: 573 482 4226
Fragments arranged by size:
4226 573 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 3492 4520 1540
Size: 275 186 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 186
BsiHKAI G_wGCw'C
Cuts at: 3342 3427 4498 3342
Size: 85 1071 4125
Fragments arranged by size:
4125 1071 85
BsmI GAATG_Cn'
Cuts at: 2792 2885 2792
Size: 93 5188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2600 3342 3427 4498 1391
Size: 399 733 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 85 77
BspEI T'CCGG_A
Cuts at: 4944 4944
Size: 5281
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 844
Size: 1792 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 1394
Size: 429 1345 3507
Fragments arranged by size:
3507 1345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2309 3776 3950 65
Size: 1183 1061 1467 174 1396
Fragments arranged by size:
1467 1396 1183 1061 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3925 4112 1700
Size: 110 2115 187 2869
Fragments arranged by size:
2869 2115 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 2165 1925
Size: 240 5041
BssSI C'ACGA_G
Cuts at: 3341 4353 3341
Size: 1012 4269
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5281
BstBI TT'CG_AA
Cuts at: 2542 4085 2542
Size: 1543 3738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstYI r'GATC_y
Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276
Size: 639 367 385 17 1452 86 12 346
Cuts at: 5276 1972
Size: 1977
Fragments arranged by size:
1977 1452 639 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2179 2205 513
Size: 552 939 175 26 3589
Fragments arranged by size:
3589 939 552 175 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3591 3611 2797
Size: 794 20 4467
Fragments arranged by size:
4467 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2352 2387 529
Size: 35 1788 35 3423
Fragments arranged by size:
3423 1788 35 35
DraI TTT'AAA
Cuts at: 2407 2560 2585 2407
Size: 153 25 5103
Fragments arranged by size:
5103 153 25
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2182 2728
Size: 54 1425 50 275 18 177 175 546
Cuts at: 2728 3617 5246 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 546 275 177 175 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 1537
Size: 275 916 4090
Fragments arranged by size:
4090 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3211 1353
Size: 1858 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 3344 3941 4728 4996 1239
Size: 393 918 794 597 787 268 1524
Fragments arranged by size:
1524 918 794 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2191 2191
Size: 5281
EcoRI G'AATT_C
Cuts at: 2590 2968 2590
Size: 378 4903
FspI TGC'GCA
Cuts at: 3787 3787
Size: 5281
HaeII r_GCGC'y
Cuts at: 1678 2052 2088 4428 1678
Size: 374 36 2340 2531
Fragments arranged by size:
2531 2340 374 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3922 3954 3996 4028 1373
Size: 32 135 32 2350 32 42 32 2626
Fragments arranged by size:
2626 2350 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KasI G'GCGC_C
Cuts at: 2048 2084 2048
Size: 36 5245
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2880 2880
Size: 5281
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3459
Fragments arranged by size:
3459 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3239 3598 3757 518
Size: 648 1191 882 359 159 2042
Fragments arranged by size:
2042 1191 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2232 2570 2697 3374 4522 4767 1606
Size: 575 51 338 127 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 575 338 245 127 51
NarI GG'CG_CC
Cuts at: 2049 2085 2049
Size: 36 5245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3790
Fragments arranged by size:
3790 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2380 2639 4184 1132
Size: 97 1151 259 1545 2229
Fragments arranged by size:
2229 1545 1151 259 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5281
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5281
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5281
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5281
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2633 830
Size: 1803 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5281
PvuII CAG'CTG
Cuts at: 2232 2570 2232
Size: 338 4943
SacII CC_GC'GG
Cuts at: 2182 2182
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2629 3764 4445 4636 826
Size: 219 1584 1135 681 191 1471
Fragments arranged by size:
1584 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2050 2086 2050
Size: 36 5245
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2054 2608 1872
Size: 182 554 4545
Fragments arranged by size:
4545 554 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3385 4286 4548 820
Size: 197 926 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5281
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2720 513
Size: 552 939 333 383 3074
Fragments arranged by size:
3074 939 552 383 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3308 3325 3478 3663 4108 325
Size: 1255 1728 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3527 5239 96
Size: 274 80 33 51 1443 1550 1712 138
Fragments arranged by size:
1712 1550 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5241 5273 5241
Size: 32 5249
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5281
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2077
Size: 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2052 2606 1870
Size: 182 554 4545
Fragments arranged by size:
4545 554 182
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3089 276
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI