Restriction map for
pFN21AE2524
(Circular) (Six-base) MAPSORT of: pFN21AE2524.seq Check: 1248 from: 1 to: 6076
pFN21AE2524 6076 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 1937
Size: 1482 4594
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2673 3210 4139 5523 5791 1632
Size: 1041 537 929 1384 268 1917
Fragments arranged by size:
1917 1384 1041 929 537 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 4975 5952 1203
Size: 3772 977 1327
Fragments arranged by size:
3772 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 2134 4805 2134
Size: 2671 3405
AleI CACnn'nnGTG
Cuts at: 1166 2343 3045 1166
Size: 1177 702 4197
Fragments arranged by size:
4197 1177 702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6076
AlwNI CAG_nnn'CTG
Cuts at: 2980 3819 5391 2980
Size: 839 1572 3665
Fragments arranged by size:
3665 1572 839
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 2015 2513 2795 2962 3305 3385 3611 3763
Size: 522 498 282 167 343 80 226 152
Cuts at: 3763 5872 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 522 498 343 282 226 167
152 80
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2132 2632 3401 1870
Size: 73 108 81 500 769 4545
Fragments arranged by size:
4545 769 500 108 81 73
BamHI G'GATC_C
Cuts at: 2486 3406 3773 2486
Size: 920 367 4789
Fragments arranged by size:
4789 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2306 3397 4852 618
Size: 325 217 627 305 214 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 627 325 305 217 214
BanII G_rGCy'C
Cuts at: 1391 2043 2259 3395 1391
Size: 652 216 1136 4072
Fragments arranged by size:
4072 1136 652 216
BbeI G_GCGC'C
Cuts at: 2096 2310 2096
Size: 214 5862
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5105
BbvCI CC'TCA_GC
Cuts at: 2526 2526
Size: 6076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4267 4301 1903
Size: 34 2330 34 3678
Fragments arranged by size:
3678 2330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3120 3968 5178 3120
Size: 848 1210 4018
Fragments arranged by size:
4018 1210 848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2053 4687 136
Size: 107 122 71 631 605 381 2634 1525
Fragments arranged by size:
2634 1525 631 605 381 122 107 71
BglII A'GATC_T
Cuts at: 6071 6071
Size: 6076
BlpI GC'TnA_GC
Cuts at: 2063 3493 2063
Size: 1430 4646
Bme1580I G_kGCm'C
Cuts at: 1790 4137 5293 1790
Size: 2347 1156 2573
Fragments arranged by size:
2573 2347 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4765 442
Size: 416 53 83 399 24 2390 958 1753
Fragments arranged by size:
2390 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 2639 2883 4736 1239
Size: 1322 78 244 1853 2579
Fragments arranged by size:
2579 1853 1322 244 78
Bpu10I CC'TnA_GC
Cuts at: 2363 2526 2363
Size: 163 5913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4201 5066 5364 4201
Size: 865 298 4913
Fragments arranged by size:
4913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3867 493
Size: 711 2663 2702
Fragments arranged by size:
2702 2663 711
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2093 2118 2307 3883
Size: 53 83 186 936 560 25 189 1576
Cuts at: 3883 4265 4900 275
Size: 382 635 1451
Fragments arranged by size:
1576 1451 936 635 560 382 189 186
83 53 25
BsaWI w'CCGG_w
Cuts at: 3102 4509 4907 5181 5328 5739 3102
Size: 1407 398 274 147 411 3439
Fragments arranged by size:
3439 1407 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2881 2911 3114 3144 1178
Size: 30 165 30 1478 30 203 30 4110
Fragments arranged by size:
4110 1478 203 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6076
BseYI C'CCAG_C
Cuts at: 2146 2214 5279 5775 2146
Size: 68 3065 496 2447
Fragments arranged by size:
3065 2447 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2005 1163
Size: 573 269 5234
Fragments arranged by size:
5234 573 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3526 4287 5315 1540
Size: 275 186 1525 761 1028 2301
Fragments arranged by size:
2301 1525 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2387 2436 4137 4222 5293 2387
Size: 49 1701 85 1071 3170
Fragments arranged by size:
3170 1701 1071 85 49
BsmI GAATG_Cn'
Cuts at: 3158 3587 3680 3158
Size: 429 93 5554
Fragments arranged by size:
5554 429 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2259 2387 2436 3395 4137 4222
Size: 399 253 216 128 49 959 742 85
Cuts at: 4222 5293 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 959 742 399 253 216 128
85 49
BspEI T'CCGG_A
Cuts at: 3102 5739 3102
Size: 2637 3439
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 844
Size: 2587 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2167 3963 1394
Size: 429 227 117 1796 3507
Fragments arranged by size:
3507 1796 429 227 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 2876 4571 4745 65
Size: 1183 1628 1695 174 1396
Fragments arranged by size:
1695 1628 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2637 4720 4907 1700
Size: 110 827 2083 187 2869
Fragments arranged by size:
2869 2083 827 187 110
BsrGI T'GTAC_A
Cuts at: 96 3280 96
Size: 3184 2892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 2997 4880 2997
Size: 1883 4193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2539 2146
Size: 393 5683
BstYI r'GATC_y
Cuts at: 1972 2486 3406 3773 4158 4175 5627 5713 5725
Size: 514 920 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 920 514 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 4057
Fragments arranged by size:
4057 1467 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 3592 4386 4406 3592
Size: 794 20 5262
Fragments arranged by size:
5262 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 3523 4412
Size: 54 1425 50 275 18 322 1371 889
Cuts at: 4412 6041 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1371 889 322 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3353 4006 1353
Size: 2000 653 3423
Fragments arranged by size:
3423 2000 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2172 4663 5037 5183 387
Size: 1064 138 462 121 2491 374 146 1280
Fragments arranged by size:
2491 1280 1064 462 374 146 138 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2561 2639 2673 2883 3210 4139 4736
Size: 393 929 78 34 210 327 929 597
Cuts at: 4736 5523 5791 1239
Size: 787 268 1524
Fragments arranged by size:
1524 929 929 787 597 393 327 268
210 78 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 6076
EcoO109I rG'GnC_Cy
Cuts at: 2274 2317 2469 2274
Size: 43 152 5881
Fragments arranged by size:
5881 152 43
EcoRI G'AATT_C
Cuts at: 2962 3385 3763 2962
Size: 423 378 5275
Fragments arranged by size:
5275 423 378
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2096 2310 5223 1678
Size: 418 214 2913 2531
Fragments arranged by size:
2913 2531 418 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 3084 3116 4717
Size: 32 135 32 548 32 932 32 1601
Cuts at: 4717 4749 4791 4823 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1601 932 548 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3365 748
Size: 2617 3459
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KasI G'GCGC_C
Cuts at: 2092 2306 2092
Size: 214 5862
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5190 5374 1305
Size: 3885 184 2007
Fragments arranged by size:
3885 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4254
Fragments arranged by size:
4254 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 3045 4034 4393 4552 518
Size: 648 1177 702 989 359 159 2042
Fragments arranged by size:
2042 1177 989 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2191 2197 2203 2209 3492 4169 5317
Size: 540 45 6 6 6 1283 677 1148
Cuts at: 5317 5562 1606
Size: 245 2120
Fragments arranged by size:
2120 1283 1148 677 540 245 45 6
6 6
NaeI GCC'GGC
Cuts at: 2639 2639
Size: 6076
NarI GG'CG_CC
Cuts at: 2093 2307 2093
Size: 214 5862
NcoI C'CATG_G
Cuts at: 513 1065 2532 513
Size: 552 1467 4057
Fragments arranged by size:
4057 1467 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6076
NgoMIV G'CCGG_C
Cuts at: 2637 2637
Size: 6076
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NspI r_CATG'y
Cuts at: 1132 1229 2374 3434 4979 1132
Size: 97 1145 1060 1545 2229
Fragments arranged by size:
2229 1545 1145 1060 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6076
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2318 2350 4145 4177 5658 5690 2318
Size: 32 1795 32 1481 32 2704
Fragments arranged by size:
2704 1795 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2469 2469
Size: 6076
PshAI GACnn'nnGTC
Cuts at: 1613 3271 1613
Size: 1658 4418
PsiI TTA'TAA
Cuts at: 3206 3646 3206
Size: 440 5636
PspXI vC'TCGA_Gb
Cuts at: 2132 2132
Size: 6076
PstI C_TGCA'G
Cuts at: 830 2683 3428 830
Size: 1853 745 3478
Fragments arranged by size:
3478 1853 745
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 3187 4324 6036 3187
Size: 1137 1712 3227
Fragments arranged by size:
3227 1712 1137
SfcI C'TryA_G
Cuts at: 826 1045 2679 3424 4559 5240 5431 826
Size: 219 1634 745 1135 681 191 1471
Fragments arranged by size:
1634 1471 1135 745 681 219 191
SfoI GGC'GCC
Cuts at: 2094 2308 2094
Size: 214 5862
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 4180 5081 5343 820
Size: 197 926 189 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6076
StyI C'CwwG_G
Cuts at: 513 1065 2410 2532 2608 2853 3515 513
Size: 552 1345 122 76 245 662 3074
Fragments arranged by size:
3074 1345 662 552 245 122 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4103 4120 4273 4458 4903 325
Size: 1255 2523 17 153 185 445 1498
Fragments arranged by size:
2523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3185 3280 4322
Size: 274 80 33 51 1443 1208 95 1042
Cuts at: 4322 6034 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1208 1042 274 138 95 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6036 6068 6036
Size: 32 6044
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XhoI C'TCGA_G
Cuts at: 1943 2132 1943
Size: 189 5887
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 4205 4205
Size: 6076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI