Restriction map for
pFN21AE2519
(Circular) (Six-base) MAPSORT of: pFN21AE2519.seq Check: 2000 from: 1 to: 6370
pFN21AE2519 6370 bp
With 182 enzymes: SgfI *
April 4, 2014 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2654 2654
Size: 6370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3537 3713 1937
Size: 1600 176 4594
Fragments arranged by size:
4594 1600 176
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3160 3347 3383 3494 3537 4433 5817 6085
Size: 1528 187 36 111 43 896 1384 268
Cuts at: 6085 1632
Size: 1917
Fragments arranged by size:
1917 1528 1384 896 268 187 111 43
36
AfeI AGC'GCT
Cuts at: 2435 3272 2435
Size: 837 5533
AflII C'TTAA_G
Cuts at: 820 1017 3659 820
Size: 197 2642 3531
Fragments arranged by size:
3531 2642 197
AflIII A'CryG_T
Cuts at: 1203 2211 5269 6246 1203
Size: 1008 3058 977 1327
Fragments arranged by size:
3058 1327 1008 977
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2805 1184
Size: 1621 4749
AlwNI CAG_nnn'CTG
Cuts at: 3212 4113 5685 3212
Size: 901 1572 3897
Fragments arranged by size:
3897 1572 901
ApaLI G'TGCA_C
Cuts at: 3251 4427 5583 3251
Size: 1176 1156 4038
Fragments arranged by size:
4038 1176 1156
ApoI r'AATT_y
Cuts at: 1493 2195 3645 3679 3905 4057 6166 1493
Size: 702 1450 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1450 702 226 152 34
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3695 1870
Size: 73 1752 4545
Fragments arranged by size:
4545 1752 73
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 3691 5146 618
Size: 325 217 627 632 1272 1455 1842
Fragments arranged by size:
1842 1455 1272 632 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2573 3689 1391
Size: 1182 1116 4072
Fragments arranged by size:
4072 1182 1116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2713 3467 928
Size: 971 814 754 3831
Fragments arranged by size:
3831 971 814 754
BbvCI CC'TCA_GC
Cuts at: 3482 3482
Size: 6370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3273 3307 3589 3623 4561 4595 1903
Size: 34 1336 34 282 34 938 34 3678
Fragments arranged by size:
3678 1336 938 282 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3293 4981 136
Size: 107 122 71 631 605 1621 1688 1525
Fragments arranged by size:
1688 1621 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3054 6365 3054
Size: 3311 3059
BlpI GC'TnA_GC
Cuts at: 3787 3787
Size: 6370
Bme1580I G_kGCm'C
Cuts at: 1790 3017 3131 3255 4431 5587 1790
Size: 1227 114 124 1176 1156 2573
Fragments arranged by size:
2573 1227 1176 1156 124 114
BmgBI CAC'GTC
Cuts at: 1318 2812 3607 1318
Size: 1494 795 4081
Fragments arranged by size:
4081 1494 795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2112 2735 4101
Size: 416 53 83 399 24 695 623 1366
Cuts at: 4101 5059 442
Size: 958 1753
Fragments arranged by size:
1753 1366 958 695 623 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2348 3121 3239 3244 3297 5030 1239
Size: 841 268 773 118 5 53 1733 2579
Fragments arranged by size:
2579 1733 841 773 268 118 53 5
Bpu10I CC'TnA_GC
Cuts at: 3482 3482
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2062 2228 4495 5360 5658 2062
Size: 166 2267 865 298 2774
Fragments arranged by size:
2774 2267 865 298 166
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5033 882
Size: 617 3534 2219
Fragments arranged by size:
3534 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4161 493
Size: 711 2957 2702
Fragments arranged by size:
2957 2702 711
BsaBI GATnn'nnATC
Cuts at: 3053 6364 3053
Size: 3311 3059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3150 3224 4177 4559
Size: 53 83 186 936 1617 74 953 382
Cuts at: 4559 5194 275
Size: 635 1451
Fragments arranged by size:
1617 1451 953 936 635 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 3111 4803 5201 5475 5622 6033 3111
Size: 1692 398 274 147 411 3448
Fragments arranged by size:
3448 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6145
Fragments arranged by size:
6145 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 3368 3449 1186
Size: 1684 498 81 4107
Fragments arranged by size:
4107 1684 498 81
BseYI C'CCAG_C
Cuts at: 2318 2537 2659 3302 5573 6069 2318
Size: 219 122 643 2271 496 2619
Fragments arranged by size:
2619 2271 643 496 219 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5797
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3820 4581 5609 1540
Size: 275 186 1819 761 1028 2301
Fragments arranged by size:
2301 1819 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2850 2939 3255 4431 4516 5587 2850
Size: 89 316 1176 85 1071 3633
Fragments arranged by size:
3633 1176 1071 316 89 85
BsmI GAATG_Cn'
Cuts at: 2409 2773 3493 3881 3974 2409
Size: 364 720 388 93 4805
Fragments arranged by size:
4805 720 388 364 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2147 3182 3215 2147
Size: 1035 33 5302
Fragments arranged by size:
5302 1035 33
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 2850 2939 3017 3131 3255 3689
Size: 399 783 277 89 78 114 124 434
Cuts at: 3689 4431 4516 5587 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 434 399 277 124
114 89 85 78
BspEI T'CCGG_A
Cuts at: 3111 6033 3111
Size: 2922 3448
BspHI T'CATG_A
Cuts at: 2190 4259 5989 2190
Size: 2069 1730 2571
Fragments arranged by size:
2571 2069 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 2991 3344 3725 844
Size: 1810 337 353 381 3489
Fragments arranged by size:
3489 1810 381 353 337
BsrBI CCG'CTC
Cuts at: 1394 1823 3100 4257 1394
Size: 429 1277 1157 3507
Fragments arranged by size:
3507 1277 1157 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3190 4865 5039 65
Size: 1183 1942 1675 174 1396
Fragments arranged by size:
1942 1675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2333 5014 5201 1700
Size: 110 523 2681 187 2869
Fragments arranged by size:
2869 2681 523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6370
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 3054 3700 4067 4452 4469 5921 6007 6019
Size: 1082 646 367 385 17 1452 86 12
Cuts at: 6019 6365 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1082 646 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2137 2610 513
Size: 552 1072 473 4273
Fragments arranged by size:
4273 1072 552 473
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3105 487
Size: 683 56 1879 3752
Fragments arranged by size:
3752 1879 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3886 4680 4700 2787
Size: 1099 794 20 4457
Fragments arranged by size:
4457 1099 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3203 3817 4706
Size: 54 1425 50 275 18 1373 614 889
Cuts at: 4706 6335 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1373 889 614 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2895 3513 4300 1353
Size: 1136 406 618 787 3423
Fragments arranged by size:
3423 1136 787 618 406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4957 5331 5477 387
Size: 1064 138 3368 374 146 1280
Fragments arranged by size:
3368 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2348 3121 3160 3239 3244 3297
Size: 393 448 268 773 39 79 5 53
Cuts at: 3297 3347 3383 3494 3537 4433 5030 5817 6085
Size: 50 36 111 43 896 597 787 268
Cuts at: 6085 1239
Size: 1524
Fragments arranged by size:
1524 896 787 773 597 448 393 268
268 111 79 53 50 43 39 36
5
EcoO109I rG'GnC_Cy
Cuts at: 2069 2274 2315 2737 3299 2069
Size: 205 41 422 562 5140
Fragments arranged by size:
5140 562 422 205 41
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
EcoRV GAT'ATC
Cuts at: 2494 2494
Size: 6370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3413 3413
Size: 6370
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 2437 3025 3274 5517 1678
Size: 759 588 249 2243 2531
Fragments arranged by size:
2531 2243 759 588 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2369 2401 2933
Size: 32 135 32 626 32 139 32 532
Cuts at: 2933 2965 5011 5043 5085 5117 1373
Size: 32 2046 32 42 32 2626
Fragments arranged by size:
2626 2046 626 532 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3568 3714 3960 669
Size: 988 281 1630 146 246 3079
Fragments arranged by size:
3079 1630 988 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6370
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2715 3336 5484 5668 1305
Size: 1410 621 2148 184 2007
Fragments arranged by size:
2148 2007 1410 621 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4548
Fragments arranged by size:
4548 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2810 4328 4687 4846 518
Size: 648 1203 441 1518 359 159 2042
Fragments arranged by size:
2042 1518 1203 648 441 359 159
MspA1I CmG'CkG
Cuts at: 1606 2752 2800 3786 4463 5611 5856 1606
Size: 1146 48 986 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 986 677 245 48
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 6370
NcoI C'CATG_G
Cuts at: 513 1065 2137 2610 513
Size: 552 1072 473 4273
Fragments arranged by size:
4273 1072 552 473
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 3728 5273 1132
Size: 97 2499 1545 2229
Fragments arranged by size:
2499 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2566 1348
Size: 1218 5152
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 2666 1108
Size: 1035 523 4812
Fragments arranged by size:
4812 1035 523
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 2737 3299 2069
Size: 668 562 5140
Fragments arranged by size:
5140 668 562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 2314 2988 3270 3722 830
Size: 1484 674 282 452 3478
Fragments arranged by size:
3478 1484 674 452 282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2752 2752
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SanDI GG'GwC_CC
Cuts at: 2069 2069
Size: 6370
SapI GCTCTTCn'nnn_
Cuts at: 2489 2489
Size: 6370
SbfI CC_TGCA'GG
Cuts at: 2314 3722 2314
Size: 1408 4962
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 2310 2984 3266 3718 4853 5534 5725
Size: 219 1265 674 282 452 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1265 1135 681 674 452 282 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 2243 3659 4474 5375 5637
Size: 197 926 98 202 1416 815 901 262
Cuts at: 5637 820
Size: 1553
Fragments arranged by size:
1553 1416 926 901 815 262 202 197
98
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2676 1517
Size: 1159 5211
StyI C'CwwG_G
Cuts at: 513 1065 2073 2119 2137 2610 2907 3384 3809
Size: 552 1008 46 18 473 297 477 425
Cuts at: 3809 513
Size: 3074
Fragments arranged by size:
3074 1008 552 477 473 425 297 46
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2623 4397 4414 4567 4752 5197 325
Size: 1255 1043 1774 17 153 185 445 1498
Fragments arranged by size:
1774 1498 1255 1043 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4616 6328 96
Size: 274 80 33 51 1443 2639 1712 138
Fragments arranged by size:
2639 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 6330 6362 2759
Size: 32 3539 32 2767
Fragments arranged by size:
3539 2767 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 3107 4499 3107
Size: 1392 4978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI XcmI