Restriction map for
pFN21AE2518
(Circular) (Six-base) MAPSORT of: pFN21AE2518.seq Check: 9791 from: 1 to: 9073
pFN21AE2518 9073 bp
With 182 enzymes: SgfI *
February 23, 2015 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2406 3182 2406
Size: 776 8297
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6883 278
Size: 53 83 186 936 5347 2468
Fragments arranged by size:
5347 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 6416 1937
Size: 537 3942 4594
Fragments arranged by size:
4594 3942 537
Acc65I G'GTAC_C
Cuts at: 6394 6394
Size: 9073
AclI AA'CG_TT
Cuts at: 1563 3335 7200 7573 1563
Size: 1772 3865 373 3063
Fragments arranged by size:
3865 3063 1772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4155 4425 7136 8520 8788 1632
Size: 2523 270 2711 1384 268 1917
Fragments arranged by size:
2711 2523 1917 1384 270 268
AfeI AGC'GCT
Cuts at: 2105 2105
Size: 9073
AflII C'TTAA_G
Cuts at: 820 1017 3673 820
Size: 197 2656 6220
Fragments arranged by size:
6220 2656 197
AflIII A'CryG_T
Cuts at: 1203 4650 5058 5458 6247 7972 8949 1203
Size: 3447 408 400 789 1725 977 1327
Fragments arranged by size:
3447 1725 1327 977 789 408 400
AgeI A'CCGG_T
Cuts at: 7904 7904
Size: 9073
AhdI GACnn_n'nnGTC
Cuts at: 5084 7802 5084
Size: 2718 6355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3636 3668 4180 4212 5046 5078 3636
Size: 32 512 32 834 32 7631
Fragments arranged by size:
7631 834 512 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3010 4403 5025 6816 8388 3010
Size: 1393 622 1791 1572 3695
Fragments arranged by size:
3695 1791 1572 1393 622
ApaLI G'TGCA_C
Cuts at: 6251 7130 8286 6251
Size: 879 1156 7038
Fragments arranged by size:
7038 1156 879
ApoI r'AATT_y
Cuts at: 1493 2174 2291 2639 3046 3221 3772 3869 4495
Size: 681 117 348 407 175 551 97 626
Cuts at: 4495 4717 4852 5342 6382 6608 6760 8869 1493
Size: 222 135 490 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 681 626 551 490 407
348 226 222 175 152 135 117 97
AseI AT'TA_AT
Cuts at: 160 1032 4109 4305 7627 160
Size: 872 3077 196 3322 1606
Fragments arranged by size:
3322 3077 1606 872 196
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9073
AvaI C'yCGr_G
Cuts at: 1870 1943 4032 6398 1870
Size: 73 2089 2366 4545
Fragments arranged by size:
4545 2366 2089 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4771 4804 4771
Size: 33 9040
BamHI G'GATC_C
Cuts at: 5407 6403 6770 5407
Size: 996 367 7710
Fragments arranged by size:
7710 996 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2724 3956 6269 6394 7849
Size: 325 217 627 937 1232 2313 125 1455
Cuts at: 7849 618
Size: 1842
Fragments arranged by size:
2313 1842 1455 1232 937 627 325 217
125
BanII G_rGCy'C
Cuts at: 1391 2766 4051 5556 6392 1391
Size: 1375 1285 1505 836 4072
Fragments arranged by size:
4072 1505 1375 1285 836
BbeI G_GCGC'C
Cuts at: 3960 6273 3960
Size: 2313 6760
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3639 5609 5786 6009 6150 928
Size: 971 1740 1970 177 223 141 3851
Fragments arranged by size:
3851 1970 1740 971 223 177 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3178 3212 4467 4501 7264 7298 1903
Size: 34 1241 34 1255 34 2763 34 3678
Fragments arranged by size:
3678 2763 1255 1241 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 6965 8175 3215
Size: 3750 1210 4113
Fragments arranged by size:
4113 3750 1210
BclI T'GATC_A
Cuts at: 1548 4599 1548
Size: 3051 6022
BfrBI ATG'CAT
Cuts at: 5423 5423
Size: 9073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7684 136
Size: 107 122 71 631 605 6012 1525
Fragments arranged by size:
6012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2424 6331 9068 2424
Size: 3907 2737 2429
Fragments arranged by size:
3907 2737 2429
BlpI GC'TnA_GC
Cuts at: 2760 6490 2760
Size: 3730 5343
Bme1580I G_kGCm'C
Cuts at: 1790 6255 7134 8290 1790
Size: 4465 879 1156 2573
Fragments arranged by size:
4465 2573 1156 879
BmgBI CAC'GTC
Cuts at: 1318 5922 6248 1318
Size: 4604 326 4143
Fragments arranged by size:
4604 4143 326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 2260 3020
Size: 416 53 83 399 24 704 139 760
Cuts at: 3020 3430 3895 4228 5040 5110 5548 5779 6804
Size: 410 465 333 812 70 438 231 1025
Cuts at: 6804 7762 442
Size: 958 1753
Fragments arranged by size:
1753 1025 958 812 760 704 465 438
416 410 399 333 231 139 83 70
53 24
BmtI G_CTAG'C
Cuts at: 1056 4071 1056
Size: 3015 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3180 3180
Size: 9073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2437 5259 7733 1239
Size: 1198 2822 2474 2579
Fragments arranged by size:
2822 2579 2474 1198
Bpu10I CC'TnA_GC
Cuts at: 2768 4365 2768
Size: 1597 7476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4368 6246 7198 8063 8361 4368
Size: 1878 952 865 298 5080
Fragments arranged by size:
5080 1878 952 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4963 5953 7736 882
Size: 617 3464 990 1783 2219
Fragments arranged by size:
3464 2219 1783 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 4651 6864 493
Size: 711 3447 2213 2702
Fragments arranged by size:
3447 2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 9067 9067
Size: 9073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3957 6270 6880 7262
Size: 53 83 186 936 2424 2313 610 382
Cuts at: 7262 7897 275
Size: 635 1451
Fragments arranged by size:
2424 2313 1451 936 635 610 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3943 5944 7506 7904 8178 8325 8736 3943
Size: 2001 1562 398 274 147 411 4280
Fragments arranged by size:
4280 2001 1562 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 3910 3940 4180
Size: 30 165 30 601 30 1876 30 240
Cuts at: 4180 4210 4363 4393 5257 5287 1178
Size: 30 153 30 864 30 4964
Fragments arranged by size:
4964 1876 864 601 240 165 153 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4371 5097 1186
Size: 3185 726 5162
Fragments arranged by size:
5162 3185 726
BseYI C'CCAG_C
Cuts at: 2558 3240 8276 8772 2558
Size: 682 5036 496 2859
Fragments arranged by size:
5036 2859 682 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2567 6523 7284 8312 1540
Size: 275 186 566 3956 761 1028 2301
Fragments arranged by size:
3956 2301 1028 761 566 275 186
BsiHKAI G_wGCw'C
Cuts at: 2453 2766 4051 6255 7134 7219 8290 2453
Size: 313 1285 2204 879 85 1071 3236
Fragments arranged by size:
3236 2204 1285 1071 879 313 85
BsmI GAATG_Cn'
Cuts at: 2510 3616 5400 6584 6677 2510
Size: 1106 1784 1184 93 4906
Fragments arranged by size:
4906 1784 1184 1106 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2766 4051 5556 6255 6392 7134
Size: 399 663 313 1285 1505 699 137 742
Cuts at: 7134 7219 8290 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1505 1285 1071 742 699 663 399
313 137 85
BspEI T'CCGG_A
Cuts at: 3943 8736 3943
Size: 4793 4280
BspHI T'CATG_A
Cuts at: 2785 5331 6103 6962 8692 2785
Size: 2546 772 859 1730 3166
Fragments arranged by size:
3166 2546 1730 859 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 3182 5222 6428 844
Size: 1562 776 2040 1206 3489
Fragments arranged by size:
3489 2040 1562 1206 776
BsrBI CCG'CTC
Cuts at: 1394 1823 6960 1394
Size: 429 5137 3507
Fragments arranged by size:
5137 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2494 5611 5623 7568 7742 65
Size: 1183 1246 3117 12 1945 174 1396
Fragments arranged by size:
3117 1945 1396 1246 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 3113 7717 7904 1700
Size: 110 1303 4604 187 2869
Fragments arranged by size:
4604 2869 1303 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9073
BssSI C'ACGA_G
Cuts at: 2551 6335 7133 8145 2551
Size: 3784 798 1012 3479
Fragments arranged by size:
3784 3479 1012 798
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2507 4577 794
Size: 1713 2070 5290
Fragments arranged by size:
5290 2070 1713
BstBI TT'CG_AA
Cuts at: 5797 7877 5797
Size: 2080 6993
BstEII G'GTnAC_C
Cuts at: 1184 2913 4277 5111 1184
Size: 1729 1364 834 5146
Fragments arranged by size:
5146 1729 1364 834
BstXI CCAn_nnnn'nTGG
Cuts at: 5404 5404
Size: 9073
BstYI r'GATC_y
Cuts at: 1972 2424 5407 5584 5974 6331 6403 6770 7155
Size: 452 2983 177 390 357 72 367 385
Cuts at: 7155 7172 8624 8710 8722 9068 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2983 1977 1452 452 390 385 367 357
346 177 86 72 17 12
BstZ17I GTA'TAC
Cuts at: 2475 2475
Size: 9073
Bsu36I CC'TnA_GG
Cuts at: 2827 2827
Size: 9073
BtgI C'CryG_G
Cuts at: 513 1065 4464 5728 513
Size: 552 3399 1264 3858
Fragments arranged by size:
3858 3399 1264 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8334
Fragments arranged by size:
8334 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2656 2882 3196 6589 7383 7403 2124
Size: 532 226 314 3393 794 20 3794
Fragments arranged by size:
3794 3393 794 532 314 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9038
DraI TTT'AAA
Cuts at: 2218 3045 6377 2218
Size: 827 3332 4914
Fragments arranged by size:
4914 3332 827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2850 8080 809
Size: 507 1534 5230 1802
Fragments arranged by size:
5230 1802 1534 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3104 6168 6520
Size: 54 1425 50 275 18 1274 3064 352
Cuts at: 6520 7409 9038 8
Size: 889 1629 43
Fragments arranged by size:
3064 1629 1425 1274 889 352 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6520 1537
Size: 275 4708 4090
Fragments arranged by size:
4708 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3744 7003 1353
Size: 2391 3259 3423
Fragments arranged by size:
3423 3259 2391
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7660 8034 8180 387
Size: 1064 138 6071 374 146 1280
Fragments arranged by size:
6071 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2764 4049 2764
Size: 1285 7788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 4155 4425 5259 7136 7733 8520
Size: 393 805 1718 270 834 1877 597 787
Cuts at: 8520 8788 1239
Size: 268 1524
Fragments arranged by size:
1877 1718 1524 834 805 787 597 393
270 268
EcoNI CCTnn'n_nnAGG
Cuts at: 4173 4173
Size: 9073
EcoO109I rG'GnC_Cy
Cuts at: 3324 3324
Size: 9073
EcoRI G'AATT_C
Cuts at: 3221 3772 6382 6760 3221
Size: 551 2610 378 5534
Fragments arranged by size:
5534 2610 551 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5006 5006
Size: 9073
FspI TGC'GCA
Cuts at: 7579 7579
Size: 9073
HaeII r_GCGC'y
Cuts at: 1678 2107 3960 6273 8220 1678
Size: 429 1853 2313 1947 2531
Fragments arranged by size:
2531 2313 1947 1853 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 4559 4591 4637
Size: 32 135 32 578 32 2377 32 46
Cuts at: 4637 4669 5070 5102 5257 5289 5479 5511 6165
Size: 32 401 32 155 32 190 32 654
Cuts at: 6165 6197 7714 7746 7788 7820 1373
Size: 32 1517 32 42 32 2626
Fragments arranged by size:
2626 2377 1517 654 578 401 190 155
135 46 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5959 6417 6663 669
Size: 988 281 4021 458 246 3079
Fragments arranged by size:
4021 3079 988 458 281 246
HindIII A'AGCT_T
Cuts at: 748 2656 3973 4929 5375 5778 748
Size: 1908 1317 956 446 403 4043
Fragments arranged by size:
4043 1908 1317 956 446 403
HpaI GTT'AAC
Cuts at: 6663 6663
Size: 9073
KasI G'GCGC_C
Cuts at: 3956 6269 3956
Size: 2313 6760
KpnI G_GTAC'C
Cuts at: 6398 6398
Size: 9073
MfeI C'AATT_G
Cuts at: 6672 6672
Size: 9073
MluI A'CGCG_T
Cuts at: 8949 8949
Size: 9073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 4829 5277 6186 8187 8371 1305
Size: 1242 2282 448 909 2001 184 2007
Fragments arranged by size:
2282 2007 2001 1242 909 448 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7251
Fragments arranged by size:
7251 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3138 4805 4834 5428 5616 6185 7031
Size: 648 1972 1667 29 594 188 569 846
Cuts at: 7031 7390 7549 518
Size: 359 159 2042
Fragments arranged by size:
2042 1972 1667 846 648 594 569 359
188 159 29
MspA1I CmG'CkG
Cuts at: 1606 2677 2868 2933 6489 7166 8314 8559 1606
Size: 1071 191 65 3556 677 1148 245 2120
Fragments arranged by size:
3556 2120 1148 1071 677 245 191 65
NarI GG'CG_CC
Cuts at: 3957 6270 3957
Size: 2313 6760
NcoI C'CATG_G
Cuts at: 513 1065 4464 5728 513
Size: 552 3399 1264 3858
Fragments arranged by size:
3858 3399 1264 552
NdeI CA'TA_TG
Cuts at: 387 4159 4210 5237 387
Size: 3772 51 1027 4223
Fragments arranged by size:
4223 3772 1027 51
NheI G'CTAG_C
Cuts at: 1052 4067 1052
Size: 3015 6058
NotI GC'GGCC_GC
Cuts at: 6520 6520
Size: 9073
NsiI A_TGCA'T
Cuts at: 5425 5425
Size: 9073
NspI r_CATG'y
Cuts at: 1132 1229 5574 6014 6431 7976 1132
Size: 97 4345 440 417 1545 2229
Fragments arranged by size:
4345 2229 1545 440 417 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9073
PciI A'CATG_T
Cuts at: 7972 7972
Size: 9073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5889 1108
Size: 4781 4292
PfoI T'CCnGG_A
Cuts at: 2965 2965
Size: 9073
PmeI GTTT'AAAC
Cuts at: 6377 6377
Size: 9073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 4180 4212 7142 7174 8655 8687 3743
Size: 32 405 32 2930 32 1481 32 4129
Fragments arranged by size:
4129 2930 1481 405 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3324 3324
Size: 9073
PshAI GACnn'nnGTC
Cuts at: 1613 2889 1613
Size: 1276 7797
PsiI TTA'TAA
Cuts at: 6237 6643 6237
Size: 406 8667
PstI C_TGCA'G
Cuts at: 830 3179 3518 5439 5836 6425 830
Size: 2349 339 1921 397 589 3478
Fragments arranged by size:
3478 2349 1921 589 397 339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9073
PvuII CAG'CTG
Cuts at: 2933 2933
Size: 9073
SacI G_AGCT'C
Cuts at: 2766 4051 2766
Size: 1285 7788
SalI G'TCGA_C
Cuts at: 1936 6415 1936
Size: 4479 4594
SanDI GG'GwC_CC
Cuts at: 3324 3324
Size: 9073
SbfI CC_TGCA'GG
Cuts at: 3179 6425 3179
Size: 3246 5827
ScaI AGT'ACT
Cuts at: 7321 9033 7321
Size: 1712 7361
SfcI C'TryA_G
Cuts at: 826 1045 3175 3514 4858 5413 5435 5832 6421
Size: 219 2130 339 1344 555 22 397 589
Cuts at: 6421 7556 8237 8428 826
Size: 1135 681 191 1471
Fragments arranged by size:
2130 1471 1344 1135 681 589 555 397
339 219 191 22
SfoI GGC'GCC
Cuts at: 3958 6271 3958
Size: 2313 6760
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9073
SmaI CCC'GGG
Cuts at: 1872 6400 1872
Size: 4528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4032 4383 6261 7177 8078
Size: 197 926 1730 359 351 1878 916 901
Cuts at: 8078 8340 820
Size: 262 1553
Fragments arranged by size:
1878 1730 1553 926 916 901 359 351
262 197
SnaBI TAC'GTA
Cuts at: 493 6864 493
Size: 6371 2702
SpeI A'CTAG_T
Cuts at: 152 3659 6317 152
Size: 3507 2658 2908
Fragments arranged by size:
3507 2908 2658
SphI G_CATG'C
Cuts at: 1132 6431 1132
Size: 5299 3774
SspI AAT'ATT
Cuts at: 5 52 2383 3689 6037 6346 6997 5
Size: 47 2331 1306 2348 309 651 2081
Fragments arranged by size:
2348 2331 2081 1306 651 309 47
StuI AGG'CCT
Cuts at: 1517 3679 1517
Size: 2162 6911
StyI C'CwwG_G
Cuts at: 513 1065 3481 4464 5728 6512 513
Size: 552 2416 983 1264 784 3074
Fragments arranged by size:
3074 2416 1264 983 784 552
SwaI ATTT'AAAT
Cuts at: 2218 2218
Size: 9073
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 3779 3921 4043 4401 4813 6355
Size: 1255 593 1606 142 122 358 412 1542
Cuts at: 6355 7100 7117 7270 7455 7900 325
Size: 745 17 153 185 445 1498
Fragments arranged by size:
1606 1542 1498 1255 745 593 445 412
358 185 153 142 122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3303 7319 9031
Size: 274 80 33 51 1443 1326 4016 1712
Cuts at: 9031 96
Size: 138
Fragments arranged by size:
4016 1712 1443 1326 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 3910 3942 4486 4518 4626 4658 5959
Size: 32 1718 32 544 32 108 32 1301
Cuts at: 5959 5983 5991 6015 6032 6064 9033 9065 2160
Size: 24 8 24 17 32 2969 32 2168
Fragments arranged by size:
2969 2168 1718 1301 544 108 32 32
32 32 32 32 24 24 17 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9073
XbaI T'CTAG_A
Cuts at: 6409 6409
Size: 9073
XhoI C'TCGA_G
Cuts at: 1943 4032 1943
Size: 2089 6984
XmaI C'CCGG_G
Cuts at: 1870 6398 1870
Size: 4528 4545
XmnI GAAnn'nnTTC
Cuts at: 3000 3753 4168 5793 5803 7202 3000
Size: 753 415 1625 10 1399 4871
Fragments arranged by size:
4871 1625 1399 753 415 10
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6881 276
Size: 53 83 186 936 5347 2468
Fragments arranged by size:
5347 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbvCI BsiWI BsmBI DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
XcmI