Restriction map for
pFN21AE2511
(Circular) (Six-base) MAPSORT of: pFN21AE2511.seq Check: 9573 from: 1 to: 5539
pFN21AE2511 5539 bp
With 182 enzymes: SgfI *
February 25, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2335 3602 4986 5254 1632
Size: 703 1267 1384 268 1917
Fragments arranged by size:
1917 1384 1267 703 268
AfeI AGC'GCT
Cuts at: 2348 2348
Size: 5539
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2680 3282 4854 2680
Size: 602 1572 3365
Fragments arranged by size:
3365 1572 602
ApaI G_GGCC'C
Cuts at: 2482 2620 2482
Size: 138 5401
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 1870
Size: 73 921 4545
Fragments arranged by size:
4545 921 73
BamHI G'GATC_C
Cuts at: 2691 2869 3236 2691
Size: 178 367 4994
Fragments arranged by size:
4994 367 178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2319 2860 4315 618
Size: 325 217 627 356 176 541 1455 1842
Fragments arranged by size:
1842 1455 627 541 356 325 217 176
BanII G_rGCy'C
Cuts at: 1391 2482 2620 2858 1391
Size: 1091 138 238 4072
Fragments arranged by size:
4072 1091 238 138
BbeI G_GCGC'C
Cuts at: 2323 2323
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2382 2956 2382
Size: 574 4965
Bme1580I G_kGCm'C
Cuts at: 1790 2482 2620 3600 4756 1790
Size: 692 138 980 1156 2573
Fragments arranged by size:
2573 1156 980 692 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2723 4199 1239
Size: 1013 471 1476 2579
Fragments arranged by size:
2579 1476 1013 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2320 3346 3728 4363
Size: 53 83 186 936 787 1026 382 635
Cuts at: 4363 275
Size: 1451
Fragments arranged by size:
1451 1026 936 787 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2796 3972 4370 4644 4791 5202 2796
Size: 1176 398 274 147 411 3133
Fragments arranged by size:
3133 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2048 2051 2066 2078 2081 2084 2300
Size: 847 15 3 15 12 3 3 216
Cuts at: 2300 2408 1186
Size: 108 4317
Fragments arranged by size:
4317 847 216 108 15 15 12 3
3 3
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2482 2620 2858 3600 3685 4756 1391
Size: 399 692 138 238 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 399 238 138 85
BspEI T'CCGG_A
Cuts at: 2796 5202 2796
Size: 2406 3133
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2235 2894 844
Size: 1391 659 3489
Fragments arranged by size:
3489 1391 659
BsrBI CCG'CTC
Cuts at: 1394 1823 2317 3426 1394
Size: 429 494 1109 3507
Fragments arranged by size:
3507 1109 494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 2322 4183 4370 1700
Size: 110 285 227 1861 187 2869
Fragments arranged by size:
2869 1861 285 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 2254 2766 96
Size: 2158 512 2869
Fragments arranged by size:
2869 2158 512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2166 2691 2869 3236 3621 3638 5090 5176
Size: 194 525 178 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 525 385 367 346 194 178
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2139 513
Size: 552 1074 3913
Fragments arranged by size:
3913 1074 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 2250 1554
Size: 696 4843
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2079 2106 2277 2406 2412 3469 1353
Size: 690 36 27 171 129 6 1057 3423
Fragments arranged by size:
3423 1057 690 171 129 36 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2405 4126 4500 4646 387
Size: 1064 138 816 1721 374 146 1280
Fragments arranged by size:
1721 1280 1064 816 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2335 2723 3602 4199 4986 5254
Size: 393 620 83 388 879 597 787 268
Cuts at: 5254 1239
Size: 1524
Fragments arranged by size:
1524 879 787 620 597 393 388 268
83
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2478 2478
Size: 5539
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 2555 4045 2555
Size: 1490 4049
FspAI rTGC'GCAy
Cuts at: 2555 2555
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2323 2350 4686 1678
Size: 645 27 2336 2531
Fragments arranged by size:
2531 2336 645 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373
Size: 32 135 32 2608 32 42 32 2626
Fragments arranged by size:
2626 2608 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 2883 3129 669
Size: 988 281 250 695 246 3079
Fragments arranged by size:
3079 988 695 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2319 2319
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2493 2664 2803 4653 4837 1305
Size: 684 504 171 139 1850 184 2007
Fragments arranged by size:
2007 1850 684 504 184 171 139
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2701 2832 2955 3632 4780 5025 1606
Size: 663 432 131 123 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 432 245 131 123
NarI GG'CG_CC
Cuts at: 2320 2320
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4987
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 1108
Size: 1593 3946
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2478 2616 2478
Size: 138 5401
PstI C_TGCA'G
Cuts at: 830 2241 2891 830
Size: 1411 650 3478
Fragments arranged by size:
3478 1411 650
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2701 2701
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SapI GCTCTTCn'nnn_
Cuts at: 2079 2079
Size: 5539
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SexAI A'CCwGG_T
Cuts at: 2496 2496
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2237 2887 4022 4703 4894 826
Size: 219 1192 650 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 650 219 191
SfoI GGC'GCC
Cuts at: 2321 2321
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2978 513
Size: 552 1913 3074
Fragments arranged by size:
3074 1913 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3566 3583 3736 3921 4366 325
Size: 1255 1986 17 153 185 445 1498
Fragments arranged by size:
1986 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2254 2766 3785
Size: 274 80 33 51 1443 277 512 1019
Cuts at: 3785 5497 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 512 277 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SrfI SwaI XcmI