Restriction map for
pFN21AE2510
(Circular) (Six-base) MAPSORT of: pFN21AE2510.seq Check: 1485 from: 1 to: 8083
pFN21AE2510 8083 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2251 3724 3979 4240 4303 4526 5071 2251
Size: 1473 255 261 63 223 545 5263
Fragments arranged by size:
5263 1473 545 261 255 223 63
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3528 5893 278
Size: 53 83 186 936 1992 2365 2468
Fragments arranged by size:
2468 2365 1992 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2594 5426 1937
Size: 657 2832 4594
Fragments arranged by size:
4594 2832 657
Acc65I G'GTAC_C
Cuts at: 5404 5404
Size: 8083
AclI AA'CG_TT
Cuts at: 1563 2172 6210 6583 1563
Size: 609 4038 373 3063
Fragments arranged by size:
4038 3063 609 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3852 6146 7530 7798 1632
Size: 1154 1066 2294 1384 268 1917
Fragments arranged by size:
2294 1917 1384 1154 1066 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7886
AflIII A'CryG_T
Cuts at: 1203 6982 7959 1203
Size: 5779 977 1327
Fragments arranged by size:
5779 1327 977
AgeI A'CCGG_T
Cuts at: 2337 6914 2337
Size: 4577 3506
AhdI GACnn_n'nnGTC
Cuts at: 6812 6812
Size: 8083
AleI CACnn'nnGTG
Cuts at: 1166 3518 4398 1166
Size: 2352 880 4851
Fragments arranged by size:
4851 2352 880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2897 3592 1184
Size: 1713 695 5675
Fragments arranged by size:
5675 1713 695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 8051
AlwNI CAG_nnn'CTG
Cuts at: 2197 2416 2440 2908 2998 3251 3298 3774 5826
Size: 219 24 468 90 253 47 476 2052
Cuts at: 5826 7398 2197
Size: 1572 2882
Fragments arranged by size:
2882 2052 1572 476 468 253 219 90
47 24
ApaI G_GGCC'C
Cuts at: 2082 2223 3806 4118 4691 4745 5049 5204 2082
Size: 141 1583 312 573 54 304 155 4961
Fragments arranged by size:
4961 1583 573 312 304 155 141 54
ApaLI G'TGCA_C
Cuts at: 3278 6140 7296 3278
Size: 2862 1156 4065
Fragments arranged by size:
4065 2862 1156
ApoI r'AATT_y
Cuts at: 1493 5392 5618 5770 7879 1493
Size: 3899 226 152 2109 1697
Fragments arranged by size:
3899 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6637 160
Size: 872 5605 1606
Fragments arranged by size:
5605 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2390 4396 4411 4655 5408 1870
Size: 73 178 269 2006 15 244 753 4545
Fragments arranged by size:
4545 2006 753 269 244 178 73 15
BamHI G'GATC_C
Cuts at: 3111 5413 5780 3111
Size: 2302 367 5414
Fragments arranged by size:
5414 2302 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2419 2605 3339 3409
Size: 325 217 627 609 23 186 734 70
Cuts at: 3409 3845 4181 4286 4400 5001 5155 5185 5404
Size: 436 336 105 114 601 154 30 219
Cuts at: 5404 6859 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 734 627 609 601 436 336
325 219 217 186 154 114 105 70
30 23
BanII G_rGCy'C
Cuts at: 1391 2051 2082 2223 3690 3806 4111 4118 4246
Size: 660 31 141 1467 116 305 7 128
Cuts at: 4246 4350 4691 4736 4745 5022 5049 5204 5265
Size: 104 341 45 9 277 27 155 61
Cuts at: 5265 5402 1391
Size: 137 4072
Fragments arranged by size:
4072 1467 660 341 305 277 155 141
137 128 116 104 61 45 31 27
9 7
BbeI G_GCGC'C
Cuts at: 2609 5005 2609
Size: 2396 5687
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3060 3785 928
Size: 971 1161 725 5226
Fragments arranged by size:
5226 1161 971 725
BbvCI CC'TCA_GC
Cuts at: 2830 2966 3335 3976 4460 2830
Size: 136 369 641 484 6453
Fragments arranged by size:
6453 641 484 369 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3184 3218 4979 5013 5361 5395 6274
Size: 34 1247 34 1761 34 348 34 879
Cuts at: 6274 6308 1903
Size: 34 3678
Fragments arranged by size:
3678 1761 1247 879 348 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5975 7185 5975
Size: 1210 6873
BclI T'GATC_A
Cuts at: 1548 2583 1548
Size: 1035 7048
BfrBI ATG'CAT
Cuts at: 3382 3382
Size: 8083
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2034 3353
Size: 107 122 71 631 605 337 25 1319
Cuts at: 3353 3941 6694 136
Size: 588 2753 1525
Fragments arranged by size:
2753 1525 1319 631 605 588 337 122
107 71 25
BglII A'GATC_T
Cuts at: 3899 8078 3899
Size: 4179 3904
BlpI GC'TnA_GC
Cuts at: 4236 4245 4928 5500 4236
Size: 9 683 572 6819
Fragments arranged by size:
6819 683 572 9
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2223 2991 3282 3412 3806 3848 3856
Size: 292 141 768 291 130 394 42 8
Cuts at: 3856 4118 4186 4289 4525 4691 4745 5049 5158
Size: 262 68 103 236 166 54 304 109
Cuts at: 5158 5204 6144 7300 1790
Size: 46 940 1156 2573
Fragments arranged by size:
2573 1156 940 768 394 304 292 291
262 236 166 141 130 109 103 68
54 46 42 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2076 2715 3801
Size: 416 53 83 399 24 659 639 1086
Cuts at: 3801 3803 4567 4701 4715 5120 5814 6772 442
Size: 2 764 134 14 405 694 958 1753
Fragments arranged by size:
1753 1086 958 764 694 659 639 416
405 399 134 83 53 24 14 2
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8083
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4752 4752
Size: 8083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 2253 2462 3344 3458 3659 4352 4747
Size: 951 63 209 882 114 201 693 395
Cuts at: 4747 4852 6743 1239
Size: 105 1891 2579
Fragments arranged by size:
2579 1891 951 882 693 395 209 201
114 105 63
Bpu10I CC'TnA_GC
Cuts at: 2830 2966 3335 3976 4460 4529 5016 5208 2830
Size: 136 369 641 484 69 487 192 5705
Fragments arranged by size:
5705 641 487 484 369 192 136 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4471 6208 7073 7371 4471
Size: 1737 865 298 5183
Fragments arranged by size:
5183 1737 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6746 882
Size: 617 5247 2219
Fragments arranged by size:
5247 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5874 493
Size: 711 4670 2702
Fragments arranged by size:
4670 2702 711
BsaBI GATnn'nnATC
Cuts at: 8077 8077
Size: 8083
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2546 2606 3525 3677
Size: 53 83 186 936 1013 60 919 152
Cuts at: 3677 4071 5002 5890 6272 6907 275
Size: 394 931 888 382 635 1451
Fragments arranged by size:
1451 1013 936 931 919 888 635 394
382 186 152 83 60 53
BsaWI w'CCGG_w
Cuts at: 2295 2337 2352 2371 6516 6914 7188 7335 7746
Size: 42 15 19 4145 398 274 147 411
Cuts at: 7746 2295
Size: 2632
Fragments arranged by size:
4145 2632 411 398 274 147 42 19
15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2625 2655 3425
Size: 30 165 30 1032 30 160 30 770
Cuts at: 3425 3455 3908 3938 4887 4917 1178
Size: 30 453 30 949 30 4344
Fragments arranged by size:
4344 1032 949 770 453 165 160 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2109 2522 2555 3272 3275 3929 4062 4477
Size: 923 413 33 717 3 654 133 415
Cuts at: 4477 4744 5065 5068 1186
Size: 267 321 3 4201
Fragments arranged by size:
4201 923 717 654 415 413 321 267
133 33 3 3
BseYI C'CCAG_C
Cuts at: 2072 2222 3670 3838 3952 3959 4207 4524 4624
Size: 150 1448 168 114 7 248 317 100
Cuts at: 4624 4714 4986 5339 7286 7782 2072
Size: 90 272 353 1947 496 2373
Fragments arranged by size:
2373 1947 1448 496 353 317 272 248
168 150 114 100 90 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2279 3506 4111 4734 1163
Size: 573 339 204 1227 605 623 4512
Fragments arranged by size:
4512 1227 623 605 573 339 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 2574 4009 5533 6294 7322
Size: 275 186 520 53 1435 1524 761 1028
Cuts at: 7322 1540
Size: 2301
Fragments arranged by size:
2301 1524 1435 1028 761 520 275 186
53
BsiHKAI G_wGCw'C
Cuts at: 2493 2964 3282 3970 4736 5265 6144 6229 7300
Size: 471 318 688 766 529 879 85 1071
Cuts at: 7300 2493
Size: 3276
Fragments arranged by size:
3276 1071 879 766 688 529 471 318
85
BsmI GAATG_Cn'
Cuts at: 3134 5594 5687 3134
Size: 2460 93 5530
Fragments arranged by size:
5530 2460 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2826 2826
Size: 8083
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2082 2223 2493 2964 2991 3282
Size: 399 261 31 141 270 471 27 291
Cuts at: 3282 3412 3690 3806 3848 3856 3970 4111 4118
Size: 130 278 116 42 8 114 141 7
Cuts at: 4118 4186 4246 4289 4350 4525 4691 4736 4745
Size: 68 60 43 61 175 166 45 9
Cuts at: 4745 5022 5049 5158 5204 5265 5402 6144 6229
Size: 277 27 109 46 61 137 742 85
Cuts at: 6229 7300 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 291 278 277
270 261 175 166 141 141 137 130
116 114 109 85 68 61 61 60
46 45 43 42 31 27 27 9
8 7
BspEI T'CCGG_A
Cuts at: 2295 2371 7746 2295
Size: 76 5375 2632
Fragments arranged by size:
5375 2632 76
BspHI T'CATG_A
Cuts at: 5972 7702 5972
Size: 1730 6353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2251 3339 3426 3724 3979 4240 4303 4526
Size: 1407 1088 87 298 255 261 63 223
Cuts at: 4526 5071 5438 844
Size: 545 367 3489
Fragments arranged by size:
3489 1407 1088 545 367 298 261 255
223 87 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2749 4240 4320 4827 5970 1394
Size: 429 926 1491 80 507 1143 3507
Fragments arranged by size:
3507 1491 1143 926 507 429 80
BsrDI GCAATG_nn'
Cuts at: 65 1248 6578 6752 65
Size: 1183 5330 174 1396
Fragments arranged by size:
5330 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2287 2337 2520 2861 3051 3554 3666
Size: 110 477 50 183 341 190 503 112
Cuts at: 3666 6727 6914 1700
Size: 3061 187 2869
Fragments arranged by size:
3061 2869 503 477 341 190 187 183
112 110 50
BsrGI T'GTAC_A
Cuts at: 96 5280 96
Size: 5184 2899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8083
BssSI C'ACGA_G
Cuts at: 2847 3092 6143 7155 2847
Size: 245 3051 1012 3775
Fragments arranged by size:
3775 3051 1012 245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2908 794
Size: 2114 5969
BstBI TT'CG_AA
Cuts at: 6887 6887
Size: 8083
BstEII G'GTnAC_C
Cuts at: 1184 2263 1184
Size: 1079 7004
BstXI CCAn_nnnn'nTGG
Cuts at: 3221 3436 3221
Size: 215 7868
BstYI r'GATC_y
Cuts at: 1972 3045 3111 3899 5113 5413 5780 6165 6182
Size: 1073 66 788 1214 300 367 385 17
Cuts at: 6182 7634 7720 7732 8078 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1214 1073 788 385 367 346
300 86 66 17 12
BstZ17I GTA'TAC
Cuts at: 2595 2595
Size: 8083
Bsu36I CC'TnA_GG
Cuts at: 4360 4360
Size: 8083
BtgI C'CryG_G
Cuts at: 513 1065 2004 2135 2840 3028 3429 3681 4476
Size: 552 939 131 705 188 401 252 795
Cuts at: 4476 513
Size: 4120
Fragments arranged by size:
4120 939 795 705 552 401 252 188
131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3912 487
Size: 683 56 2686 4658
Fragments arranged by size:
4658 2686 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2423 2571 4142 5599 6393 6413 2067
Size: 356 148 1571 1457 794 20 3737
Fragments arranged by size:
3737 1571 1457 794 356 148 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8048
DraI TTT'AAA
Cuts at: 5387 5387
Size: 8083
DraIII CAC_nnn'GTG
Cuts at: 3034 3157 3034
Size: 123 7960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7090 809
Size: 507 5774 1802
Fragments arranged by size:
5774 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2285 2518
Size: 54 1425 50 275 18 352 103 233
Cuts at: 2518 2571 2750 2859 3396 4006 4254 4888 5530
Size: 53 179 109 537 610 248 634 642
Cuts at: 5530 6419 8048 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 642 634 610 537 352
275 248 233 179 109 103 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2518 2571 4006 5530 1537
Size: 275 706 53 1435 1524 4090
Fragments arranged by size:
4090 1524 1435 706 275 53
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2744 5074 5086 6013 1353
Size: 746 645 2330 12 927 3423
Fragments arranged by size:
3423 2330 927 746 645 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2786 3541 6670 7044 7190 387
Size: 1064 138 1197 755 3129 374 146 1280
Fragments arranged by size:
3129 1280 1197 1064 755 374 146 138
EcoICRI GAG'CTC
Cuts at: 4734 5263 4734
Size: 529 7554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2253 2462 2786 3344 3458 3659
Size: 393 558 63 209 324 558 114 201
Cuts at: 3659 3852 4352 4747 4852 6146 6743 7530 7798
Size: 193 500 395 105 1294 597 787 268
Cuts at: 7798 1239
Size: 1524
Fragments arranged by size:
1524 1294 787 597 558 558 500 395
393 324 268 209 201 193 114 105
63
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 4489 5248 2537
Size: 1952 759 5372
Fragments arranged by size:
5372 1952 759
EcoO109I rG'GnC_Cy
Cuts at: 2078 2126 2219 3318 3703 3802 3803 4057 4114
Size: 48 93 1099 385 99 1 254 57
Cuts at: 4114 4356 4408 4415 4482 4505 4660 4688 4742
Size: 242 52 7 67 23 155 28 54
Cuts at: 4742 4803 4911 5045 5054 5165 5192 5200 5201
Size: 61 108 134 9 111 27 8 1
Cuts at: 5201 5274 2078
Size: 73 4887
Fragments arranged by size:
4887 1099 385 254 242 155 134 111
108 99 93 73 67 61 57 54
52 48 28 27 23 9 8 7
1 1
EcoRI G'AATT_C
Cuts at: 5392 5770 5392
Size: 378 7705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 3196 3802 4690 2716
Size: 480 606 888 6109
Fragments arranged by size:
6109 888 606 480
FseI GG_CCGG'CC
Cuts at: 2865 2865
Size: 8083
FspI TGC'GCA
Cuts at: 6589 6589
Size: 8083
HaeII r_GCGC'y
Cuts at: 1678 2609 5005 7230 1678
Size: 931 2396 2225 2531
Fragments arranged by size:
2531 2396 2225 931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2517 2531 2549
Size: 32 135 32 863 32 50 14 18
Cuts at: 2549 2563 2603 2625 2627 2635 2657 2659 3227
Size: 14 40 22 2 8 22 2 568
Cuts at: 3227 3259 3425 3457 3908 3940 4121 4153 6724
Size: 32 166 32 451 32 181 32 2571
Cuts at: 6724 6756 6798 6830 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2571 863 568 451 181 166 135
50 42 40 32 32 32 32 32
32 32 32 32 22 22 18 14
14 8 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 5032 5427 5673 669
Size: 988 281 238 2856 395 246 3079
Fragments arranged by size:
3079 2856 988 395 281 246 238
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8083
HpaI GTT'AAC
Cuts at: 5673 5673
Size: 8083
KasI G'GCGC_C
Cuts at: 2605 5001 2605
Size: 2396 5687
KpnI G_GTAC'C
Cuts at: 5408 5408
Size: 8083
MfeI C'AATT_G
Cuts at: 5682 5682
Size: 8083
MluI A'CGCG_T
Cuts at: 7959 7959
Size: 8083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3096 7197 7381 1305
Size: 1791 4101 184 2007
Fragments arranged by size:
4101 2007 1791 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2184 3398 4890 10
Size: 54 1425 343 352 1214 1492 3203
Fragments arranged by size:
3203 1492 1425 1214 352 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 2879 3245 3377 3518 4398 6041
Size: 648 1332 381 366 132 141 880 1643
Cuts at: 6041 6400 6559 518
Size: 359 159 2042
Fragments arranged by size:
2042 1643 1332 880 648 381 366 359
159 141 132
MspA1I CmG'CkG
Cuts at: 1606 2137 2834 2905 2917 3298 3576 3612 3754
Size: 531 697 71 12 381 278 36 142
Cuts at: 3754 3774 3939 4063 4471 5343 5499 6176 7324
Size: 20 165 124 408 872 156 677 1148
Cuts at: 7324 7569 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 872 697 677 531 408 381
278 245 165 156 142 124 71 36
20 12
NaeI GCC'GGC
Cuts at: 2522 2863 3556 3668 2522
Size: 341 693 112 6937
Fragments arranged by size:
6937 693 341 112
NarI GG'CG_CC
Cuts at: 2606 5002 2606
Size: 2396 5687
NcoI C'CATG_G
Cuts at: 513 1065 2004 3429 4476 513
Size: 552 939 1425 1047 4120
Fragments arranged by size:
4120 1425 1047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8083
NgoMIV G'CCGG_C
Cuts at: 2520 2861 3554 3666 2520
Size: 341 693 112 6937
Fragments arranged by size:
6937 693 341 112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8083
NotI GC'GGCC_GC
Cuts at: 2571 5530 2571
Size: 2959 5124
NsiI A_TGCA'T
Cuts at: 3384 3384
Size: 8083
NspI r_CATG'y
Cuts at: 1132 1229 2659 3382 3386 4885 5441 6986 1132
Size: 97 1430 723 4 1499 556 1545 2229
Fragments arranged by size:
2229 1545 1499 1430 723 556 97 4
PasI CC'CwG_GG
Cuts at: 1348 2039 2809 3661 4111 4175 4351 4632 4684
Size: 691 770 852 450 64 176 281 52
Cuts at: 4684 5049 5160 5196 1348
Size: 365 111 36 4235
Fragments arranged by size:
4235 852 770 691 450 365 281 176
111 64 52 36
PciI A'CATG_T
Cuts at: 6982 6982
Size: 8083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 3034 3405 1108
Size: 1308 618 371 5786
Fragments arranged by size:
5786 1308 618 371
PfoI T'CCnGG_A
Cuts at: 3114 3471 4829 3114
Size: 357 1358 6368
Fragments arranged by size:
6368 1358 357
PmeI GTTT'AAAC
Cuts at: 5387 5387
Size: 8083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2266 6152 6184 7665 7697 2234
Size: 32 3886 32 1481 32 2620
Fragments arranged by size:
3886 2620 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3318 4356 4408 4415 4482 4660 4803 4911 5054
Size: 1038 52 7 67 178 143 108 143
Cuts at: 5054 5165 5192 5274 3318
Size: 111 27 82 6127
Fragments arranged by size:
6127 1038 178 143 143 111 108 82
67 52 27 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8083
PsiI TTA'TAA
Cuts at: 5653 5653
Size: 8083
PspOMI G'GGCC_C
Cuts at: 2078 2219 3802 4114 4687 4741 5045 5200 2078
Size: 141 1583 312 573 54 304 155 4961
Fragments arranged by size:
4961 1583 573 312 304 155 141 54
PspXI vC'TCGA_Gb
Cuts at: 2121 2121
Size: 8083
PstI C_TGCA'G
Cuts at: 830 2436 2566 3144 3571 5435 830
Size: 1606 130 578 427 1864 3478
Fragments arranged by size:
3478 1864 1606 578 427 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8083
PvuII CAG'CTG
Cuts at: 2834 3298 3612 3774 5343 2834
Size: 464 314 162 1569 5574
Fragments arranged by size:
5574 1569 464 314 162
SacI G_AGCT'C
Cuts at: 4736 5265 4736
Size: 529 7554
SacII CC_GC'GG
Cuts at: 2138 2138
Size: 8083
SalI G'TCGA_C
Cuts at: 1936 5425 1936
Size: 3489 4594
SanDI GG'GwC_CC
Cuts at: 4408 4415 4660 4803 5054 5165 5192 4408
Size: 7 245 143 251 111 27 7299
Fragments arranged by size:
7299 251 245 143 111 27 7
SbfI CC_TGCA'GG
Cuts at: 3144 3571 5435 3144
Size: 427 1864 5792
Fragments arranged by size:
5792 1864 427
ScaI AGT'ACT
Cuts at: 6331 8043 6331
Size: 1712 6371
SfcI C'TryA_G
Cuts at: 826 1045 2432 2562 3140 3567 5224 5431 6566
Size: 219 1387 130 578 427 1657 207 1135
Cuts at: 6566 7247 7438 826
Size: 681 191 1471
Fragments arranged by size:
1657 1471 1387 1135 681 578 427 219
207 191 130
SfoI GGC'GCC
Cuts at: 2607 5003 2607
Size: 2396 5687
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
SgrAI Cr'CCGG_yG
Cuts at: 1700 2337 1700
Size: 637 7446
SmaI CCC'GGG
Cuts at: 1872 4398 4413 5410 1872
Size: 2526 15 997 4545
Fragments arranged by size:
4545 2526 997 15
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2121 4486 6187 7088 7350 820
Size: 197 926 178 2365 1701 901 262 1553
Fragments arranged by size:
2365 1701 1553 926 901 262 197 178
SnaBI TAC'GTA
Cuts at: 493 5874 493
Size: 5381 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8083
SphI G_CATG'C
Cuts at: 1132 3382 3386 4885 5441 1132
Size: 2250 4 1499 556 3774
Fragments arranged by size:
3774 2250 1499 556 4
SspI AAT'ATT
Cuts at: 5 52 6007 5
Size: 47 5955 2081
Fragments arranged by size:
5955 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8083
StyI C'CwwG_G
Cuts at: 513 1065 2004 3429 3699 4433 4476 4969 5522
Size: 552 939 1425 270 734 43 493 553
Cuts at: 5522 513
Size: 3074
Fragments arranged by size:
3074 1425 939 734 553 552 493 270
43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2677 3427 3443 3530 3674 4112 6110
Size: 1255 1097 750 16 87 144 438 1998
Cuts at: 6110 6127 6280 6465 6910 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 1097 750 445 438 185
153 144 87 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4776 5280 6329
Size: 274 80 33 51 1443 2799 504 1049
Cuts at: 6329 8041 96
Size: 1712 138
Fragments arranged by size:
2799 1712 1443 1049 504 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2541 2573 3143 3175 3217 3249 4887 4919 5258
Size: 32 570 32 42 32 1638 32 339
Cuts at: 5258 5290 8043 8075 2541
Size: 32 2753 32 2549
Fragments arranged by size:
2753 2549 1638 570 339 42 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2449 2639 3887 1316
Size: 1133 190 1248 5512
Fragments arranged by size:
5512 1248 1133 190
XbaI T'CTAG_A
Cuts at: 5419 5419
Size: 8083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 4277 4722 2728
Size: 1549 445 6089
Fragments arranged by size:
6089 1549 445
XhoI C'TCGA_G
Cuts at: 1943 2121 1943
Size: 178 7905
XmaI C'CCGG_G
Cuts at: 1870 4396 4411 5408 1870
Size: 2526 15 997 4545
Fragments arranged by size:
4545 2526 997 15
XmnI GAAnn'nnTTC
Cuts at: 3782 5107 6212 3782
Size: 1325 1105 5653
Fragments arranged by size:
5653 1325 1105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3526 5891 276
Size: 53 83 186 936 1992 2365 2468
Fragments arranged by size:
2468 2365 1992 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI EcoRV FspAI NruI
PacI PmlI PsrI RsrII SapI SexAI SfiI SrfI
SwaI