Restriction map for
pFN21AE2509
(Circular) (Six-base) MAPSORT of: pFN21AE2509.seq Check: 8063 from: 1 to: 6967
pFN21AE2509 6967 bp
With 182 enzymes: SgfI *
February 18, 2014 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3961 3961
Size: 6967
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2281 4310 1937
Size: 344 2029 4594
Fragments arranged by size:
4594 2029 344
Acc65I G'GTAC_C
Cuts at: 2823 4288 2823
Size: 1465 5502
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2125 2838 2964 3357 3756 3797 5030 6414
Size: 493 713 126 393 399 41 1233 1384
Cuts at: 6414 6682 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1233 713 493 399 393 268
126 41
AfeI AGC'GCT
Cuts at: 4122 4122
Size: 6967
AflII C'TTAA_G
Cuts at: 820 1017 2198 820
Size: 197 1181 5589
Fragments arranged by size:
5589 1181 197
AflIII A'CryG_T
Cuts at: 1203 2666 5866 6843 1203
Size: 1463 3200 977 1327
Fragments arranged by size:
3200 1463 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 2848 5696 2848
Size: 2848 4119
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6967
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 6935
AlwNI CAG_nnn'CTG
Cuts at: 3601 4087 4259 4710 6282 3601
Size: 486 172 451 1572 4286
Fragments arranged by size:
4286 1572 486 451 172
ApaLI G'TGCA_C
Cuts at: 5024 6180 5024
Size: 1156 5811
ApoI r'AATT_y
Cuts at: 1493 2146 2182 2236 2467 3870 4276 4502 4654
Size: 653 36 54 231 1403 406 226 152
Cuts at: 4654 6763 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1403 653 406 231 226 152
54 36
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 3672 4292 1870
Size: 73 1729 620 4545
Fragments arranged by size:
4545 1729 620 73
BamHI G'GATC_C
Cuts at: 4297 4664 4297
Size: 367 6600
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2823 4288 5743 618
Size: 325 217 627 1036 1465 1455 1842
Fragments arranged by size:
1842 1465 1455 1036 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2537 4286 1391
Size: 1146 1749 4072
Fragments arranged by size:
4072 1749 1146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2510 2844 3744 4025 4179 928
Size: 971 611 334 900 281 154 3716
Fragments arranged by size:
3716 971 900 611 334 281 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5158 5192 1903
Size: 34 3221 34 3678
Fragments arranged by size:
3678 3221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4859 6069 4859
Size: 1210 5757
BclI T'GATC_A
Cuts at: 1548 2187 2671 1548
Size: 639 484 5844
Fragments arranged by size:
5844 639 484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5578 136
Size: 107 122 71 631 605 3906 1525
Fragments arranged by size:
3906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3199 6962 3199
Size: 3763 3204
BlpI GC'TnA_GC
Cuts at: 4384 4384
Size: 6967
Bme1580I G_kGCm'C
Cuts at: 1790 5028 6184 1790
Size: 3238 1156 2573
Fragments arranged by size:
3238 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2705 4698 5656
Size: 416 53 83 399 24 1288 1993 958
Cuts at: 5656 442
Size: 1753
Fragments arranged by size:
1993 1753 1288 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2405 2850 3151 3623 3863 4034 5627
Size: 845 321 445 301 472 240 171 1593
Cuts at: 5627 1239
Size: 2579
Fragments arranged by size:
2579 1593 845 472 445 321 301 240
171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5092 5957 6255 5092
Size: 865 298 5804
Fragments arranged by size:
5804 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5630 882
Size: 617 4131 2219
Fragments arranged by size:
4131 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2411 2669 4758 493
Size: 711 1207 258 2089 2702
Fragments arranged by size:
2702 2089 1207 711 258
BsaBI GATnn'nnATC
Cuts at: 6961 6961
Size: 6967
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4774 5156 5791 275
Size: 53 83 186 936 3241 382 635 1451
Fragments arranged by size:
3241 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5400 5798 6072 6219 6630 5400
Size: 398 274 147 411 5737
Fragments arranged by size:
5737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3149 3179 1178
Size: 30 165 30 1746 30 4966
Fragments arranged by size:
4966 1746 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3330 3632 3635 4183 1186
Size: 2144 302 3 548 3970
Fragments arranged by size:
3970 2144 548 302 3
BseYI C'CCAG_C
Cuts at: 3999 6170 6666 3999
Size: 2171 496 4300
Fragments arranged by size:
4300 2171 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3929 1163
Size: 573 2193 4201
Fragments arranged by size:
4201 2193 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 5028 5113 6184 2537
Size: 2491 85 1071 3320
Fragments arranged by size:
3320 2491 1071 85
BsmI GAATG_Cn'
Cuts at: 2037 3752 4478 4571 2037
Size: 1715 726 93 4433
Fragments arranged by size:
4433 1715 726 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 4286 5028 5113 6184 1391
Size: 399 747 1749 742 85 1071 2174
Fragments arranged by size:
2174 1749 1071 747 742 399 85
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 4856 6586 4856
Size: 1730 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3961 4322 844
Size: 3117 361 3489
Fragments arranged by size:
3489 3117 361
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2021 2093 2665 5462 5636 65
Size: 1183 773 72 572 2797 174 1396
Fragments arranged by size:
2797 1396 1183 773 572 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 5611 5798 1700
Size: 110 3801 187 2869
Fragments arranged by size:
3801 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6967
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 5027 6039 5027
Size: 1012 5955
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstXI CCAn_nnnn'nTGG
Cuts at: 2129 3973 2129
Size: 1844 5123
BstYI r'GATC_y
Cuts at: 1972 2102 2958 3199 4049 4297 4664 5049 5066
Size: 130 856 241 850 248 367 385 17
Cuts at: 5066 6518 6604 6616 6962 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 856 850 385 367 346 248
241 130 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5476
Fragments arranged by size:
5476 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4146 487
Size: 683 56 2920 3308
Fragments arranged by size:
3308 2920 683 56
BtsI GCAGTG_nn'
Cuts at: 3350 4483 5277 5297 3350
Size: 1133 794 20 5020
Fragments arranged by size:
5020 1133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 2152 4271 2152
Size: 2119 4848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3579 4414 5303
Size: 54 1425 50 275 18 1749 835 889
Cuts at: 5303 6932 8
Size: 1629 43
Fragments arranged by size:
1749 1629 1425 889 835 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3413 4897 1353
Size: 2060 1484 3423
Fragments arranged by size:
3423 2060 1484
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5554 5928 6074 387
Size: 1064 138 435 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2535 2535
Size: 6967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2125 2405 2838 2850 2964 3151
Size: 393 452 41 280 433 12 114 187
Cuts at: 3151 3357 3623 3756 3797 3863 4034 5030 5627
Size: 206 266 133 41 66 171 996 597
Cuts at: 5627 6414 6682 1239
Size: 787 268 1524
Fragments arranged by size:
1524 996 787 597 452 433 393 280
268 266 206 187 171 133 114 66
41 41 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3986 3986
Size: 6967
EcoRI G'AATT_C
Cuts at: 4276 4654 4276
Size: 378 6589
FspI TGC'GCA
Cuts at: 3789 5473 3789
Size: 1684 5283
HaeII r_GCGC'y
Cuts at: 1678 2057 4124 6114 1678
Size: 379 2067 1990 2531
Fragments arranged by size:
2531 2067 1990 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2834 2866 3325
Size: 32 135 32 736 32 494 32 459
Cuts at: 3325 3357 5608 5640 5682 5714 1373
Size: 32 2251 32 42 32 2626
Fragments arranged by size:
2626 2251 736 494 459 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 4311 4557 669
Size: 988 281 625 1748 246 3079
Fragments arranged by size:
3079 1748 988 625 281 246
HindIII A'AGCT_T
Cuts at: 748 3188 748
Size: 2440 4527
HpaI GTT'AAC
Cuts at: 4557 4557
Size: 6967
KpnI G_GTAC'C
Cuts at: 2827 4292 2827
Size: 1465 5502
MfeI C'AATT_G
Cuts at: 3903 4566 3903
Size: 663 6304
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3861 6081 6265 1305
Size: 2556 2220 184 2007
Fragments arranged by size:
2556 2220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3581 10
Size: 54 1425 343 1749 3396
Fragments arranged by size:
3396 1749 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3129 3624 3930 3971 4925 5284 5443
Size: 648 1963 495 306 41 954 359 159
Cuts at: 5443 518
Size: 2042
Fragments arranged by size:
2042 1963 954 648 495 359 306 159
41
MspA1I CmG'CkG
Cuts at: 1606 3793 3961 4003 4087 4383 5060 6208 6453
Size: 2187 168 42 84 296 677 1148 245
Cuts at: 6453 1606
Size: 2120
Fragments arranged by size:
2187 2120 1148 677 296 245 168 84
42
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5476
Fragments arranged by size:
5476 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 4325 5870 1132
Size: 97 3096 1545 2229
Fragments arranged by size:
3096 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6967
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3296 4259 1108
Size: 2188 963 3816
Fragments arranged by size:
3816 2188 963
PfoI T'CCnGG_A
Cuts at: 3600 4112 3600
Size: 512 6455
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PmlI CAC'GTG
Cuts at: 2669 2669
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 2247 4537 2247
Size: 2290 4677
PstI C_TGCA'G
Cuts at: 830 3761 4083 4319 830
Size: 2931 322 236 3478
Fragments arranged by size:
3478 2931 322 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
PvuII CAG'CTG
Cuts at: 3793 3961 4003 4087 3793
Size: 168 42 84 6673
Fragments arranged by size:
6673 168 84 42
SacI G_AGCT'C
Cuts at: 2537 2537
Size: 6967
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 2641 5215 6927 2641
Size: 2574 1712 2681
Fragments arranged by size:
2681 2574 1712
SfcI C'TryA_G
Cuts at: 826 1045 3486 3757 4079 4315 5450 6131 6322
Size: 219 2441 271 322 236 1135 681 191
Cuts at: 6322 826
Size: 1471
Fragments arranged by size:
2441 1471 1135 681 322 271 236 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2198 5071 5972 6234 820
Size: 197 926 255 2873 901 262 1553
Fragments arranged by size:
2873 1553 926 901 262 255 197
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 4325 1132
Size: 3193 3774
SspI AAT'ATT
Cuts at: 5 52 4891 5
Size: 47 4839 2081
Fragments arranged by size:
4839 2081 47
StuI AGG'CCT
Cuts at: 1517 3055 1517
Size: 1538 5429
StyI C'CwwG_G
Cuts at: 513 1065 2004 4406 513
Size: 552 939 2402 3074
Fragments arranged by size:
3074 2402 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2415 2933 3653 4994 5011 5164 5349
Size: 1255 835 518 720 1341 17 153 185
Cuts at: 5349 5794 325
Size: 445 1498
Fragments arranged by size:
1498 1341 1255 835 720 518 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2639 3238 5213
Size: 274 80 33 51 1443 662 599 1975
Cuts at: 5213 6925 96
Size: 1712 138
Fragments arranged by size:
1975 1712 1443 662 599 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6927 6959 6927
Size: 32 6935
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 2037 2469 3752 3870 5096 2037
Size: 432 1283 118 1226 3908
Fragments arranged by size:
3908 1283 1226 432 118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI