Restriction map for
pFN21AE2508
(Circular) (Six-base) MAPSORT of: pFN21AE2508.seq Check: 8893 from: 1 to: 6067
pFN21AE2508 6067 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2276 3410 1937
Size: 339 1134 4594
Fragments arranged by size:
4594 1134 339
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 3024 4130 5514 5782 1632
Size: 739 653 1106 1384 268 1917
Fragments arranged by size:
1917 1384 1106 739 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3224 3810 5382 3224
Size: 586 1572 3909
Fragments arranged by size:
3909 1572 586
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2333 2405 4621 160
Size: 872 1301 72 2216 1606
Fragments arranged by size:
2216 1606 1301 872 72
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2209 3392 1870
Size: 73 266 1183 4545
Fragments arranged by size:
4545 1183 266 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3246 3386 1391
Size: 1855 140 4072
Fragments arranged by size:
4072 1855 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2841 3012 3065 928
Size: 971 138 804 171 53 3930
Fragments arranged by size:
3930 971 804 171 138 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2488 3959 5169 2488
Size: 1471 1210 3386
Fragments arranged by size:
3386 1471 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BfrBI ATG'CAT
Cuts at: 2287 2770 2287
Size: 483 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 4128 5284 1790
Size: 2338 1156 2573
Fragments arranged by size:
2573 2338 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2206 3338 3798
Size: 416 53 83 399 24 789 1132 460
Cuts at: 3798 4756 442
Size: 958 1753
Fragments arranged by size:
1753 1132 958 789 460 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4727 1239
Size: 3488 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2919 4730 882
Size: 617 1420 1811 2219
Fragments arranged by size:
2219 1811 1420 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3874 4256 4891 275
Size: 53 83 186 936 2341 382 635 1451
Fragments arranged by size:
2341 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2418 4500 4898 5172 5319 5730 2418
Size: 2082 398 274 147 411 2755
Fragments arranged by size:
2755 2082 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3302 3332 1178
Size: 30 165 30 1899 30 3913
Fragments arranged by size:
3913 1899 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6067
BseYI C'CCAG_C
Cuts at: 5270 5766 5270
Size: 496 5571
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3326 1163
Size: 573 1590 3904
Fragments arranged by size:
3904 1590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4128 4213 5284 4128
Size: 85 1071 4911
Fragments arranged by size:
4911 1071 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3246 3386 4128 4213 5284 1391
Size: 399 1456 140 742 85 1071 2174
Fragments arranged by size:
2174 1456 1071 742 399 140 85
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3049 3956 5686 3049
Size: 907 1730 3430
Fragments arranged by size:
3430 1730 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2701 3954 1394
Size: 429 878 1253 3507
Fragments arranged by size:
3507 1253 878 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2749 2998 4562 4736 65
Size: 1183 1501 249 1564 174 1396
Fragments arranged by size:
1564 1501 1396 1183 249 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 3255 96
Size: 1911 1248 2908
Fragments arranged by size:
2908 1911 1248
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062
Size: 1425 367 385 17 1452 86 12 346
Cuts at: 6062 1972
Size: 1977
Fragments arranged by size:
1977 1452 1425 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3583 4377 4397 2111
Size: 1472 794 20 3781
Fragments arranged by size:
3781 1472 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 3024 4130 4727 5514 5782 1239
Size: 393 739 653 1106 597 787 268 1524
Fragments arranged by size:
1524 1106 787 739 653 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2253 2253
Size: 6067
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
EcoRV GAT'ATC
Cuts at: 2206 2206
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4708 4740 4782 4814 1373
Size: 32 135 32 3136 32 42 32 2626
Fragments arranged by size:
3136 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3411 3657 669
Size: 988 281 1008 465 246 3079
Fragments arranged by size:
3079 1008 988 465 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3052 5181 5365 1305
Size: 1747 2129 184 2007
Fragments arranged by size:
2129 2007 1747 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 2775 3051 4025 4384 4543 518
Size: 648 1186 423 276 974 359 159 2042
Fragments arranged by size:
2042 1186 974 648 423 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2855 2911 3483 4160 5308 5553 1606
Size: 1249 56 572 677 1148 245 2120
Fragments arranged by size:
2120 1249 1148 677 572 245 56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NsiI A_TGCA'T
Cuts at: 2289 2772 2289
Size: 483 5584
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PfoI T'CCnGG_A
Cuts at: 3138 3138
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2253 2253
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3264 3296 3264
Size: 32 6035
PstI C_TGCA'G
Cuts at: 830 3419 830
Size: 2589 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2855 2911 2855
Size: 56 6011
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2142 2526 3174 3415 4550 5231 5422
Size: 219 1097 384 648 241 1135 681 191
Cuts at: 5422 826
Size: 1471
Fragments arranged by size:
1471 1135 1097 681 648 384 241 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 4171 5072 5334 820
Size: 197 926 266 1962 901 262 1553
Fragments arranged by size:
1962 1553 926 901 266 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 2343 2518 2673 3014 3991 5
Size: 47 2291 175 155 341 977 2081
Fragments arranged by size:
2291 2081 977 341 175 155 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 3506 513
Size: 552 2441 3074
Fragments arranged by size:
3074 2441 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3255 4313
Size: 274 80 33 51 1443 30 1248 1058
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1248 1058 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 6027 6059 3302
Size: 32 2693 32 3310
Fragments arranged by size:
3310 2693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 2209 1943
Size: 266 5801
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2232 4196 2232
Size: 1964 4103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI