Restriction map for
pFN21AE2505
(Circular) (Six-base) MAPSORT of: pFN21AE2505.seq Check: 4952 from: 1 to: 7825
pFN21AE2505 7825 bp
With 182 enzymes: SgfI *
February 16, 2012 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2043 2161 2485 3473 3958 2043
Size: 118 324 988 485 5910
Fragments arranged by size:
5910 988 485 324 118
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5635 278
Size: 53 83 186 936 4099 2468
Fragments arranged by size:
4099 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2328 4320 5168 1937
Size: 391 1992 848 4594
Fragments arranged by size:
4594 1992 848 391
Acc65I G'GTAC_C
Cuts at: 2234 3209 5146 2234
Size: 975 1937 4913
Fragments arranged by size:
4913 1937 975
AclI AA'CG_TT
Cuts at: 1563 5952 6325 1563
Size: 4389 373 3063
Fragments arranged by size:
4389 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2650 2718 3244 4947 5888 7272 7540
Size: 423 595 68 526 1703 941 1384 268
Cuts at: 7540 1632
Size: 1917
Fragments arranged by size:
1917 1703 1384 941 595 526 423 268
68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7628
AflIII A'CryG_T
Cuts at: 1203 3759 6724 7701 1203
Size: 2556 2965 977 1327
Fragments arranged by size:
2965 2556 1327 977
AgeI A'CCGG_T
Cuts at: 6656 6656
Size: 7825
AhdI GACnn_n'nnGTC
Cuts at: 3790 6554 3790
Size: 2764 5061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2987 1184
Size: 1803 6022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3131 3163 4034 4066 3131
Size: 32 871 32 6890
Fragments arranged by size:
6890 871 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2338 3017 4021 4925 5568 7140 2338
Size: 679 1004 904 643 1572 3023
Fragments arranged by size:
3023 1572 1004 904 679 643
ApaI G_GGCC'C
Cuts at: 4101 4398 4971 4101
Size: 297 573 6955
Fragments arranged by size:
6955 573 297
ApaLI G'TGCA_C
Cuts at: 5882 7038 5882
Size: 1156 6669
ApoI r'AATT_y
Cuts at: 1493 2405 4953 5134 5360 5512 7621 1493
Size: 912 2548 181 226 152 2109 1697
Fragments arranged by size:
2548 2109 1697 912 226 181 152
AseI AT'TA_AT
Cuts at: 160 1032 6379 160
Size: 872 5347 1606
Fragments arranged by size:
5347 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 3172 3330 4287 4397 4623 5150
Size: 73 250 979 158 957 110 226 527
Cuts at: 5150 1870
Size: 4545
Fragments arranged by size:
4545 979 957 527 250 226 158 110
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4199 4232 4199
Size: 33 7792
BamHI G'GATC_C
Cuts at: 2093 4983 5155 5522 2093
Size: 2890 172 367 4396
Fragments arranged by size:
4396 2890 367 172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2357 2909 3209 3416
Size: 325 217 627 447 123 552 300 207
Cuts at: 3416 4141 4291 4298 5069 5146 6601 618
Size: 725 150 7 771 77 1455 1842
Fragments arranged by size:
1842 1455 771 725 627 552 447 325
300 217 207 150 123 77 7
BanII G_rGCy'C
Cuts at: 1391 2871 4101 4174 4185 4398 4440 4971 5124
Size: 1480 1230 73 11 213 42 531 153
Cuts at: 5124 5144 1391
Size: 20 4072
Fragments arranged by size:
4072 1480 1230 531 213 153 73 42
20 11
BbeI G_GCGC'C
Cuts at: 4302 4302
Size: 7825
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2656 3174 4935 928
Size: 971 231 526 518 1761 3818
Fragments arranged by size:
3818 1761 971 526 518 231
BbvCI CC'TCA_GC
Cuts at: 4428 4428
Size: 7825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3983 4017 4116 4150 6016 6050 1903
Size: 34 2046 34 99 34 1866 34 3678
Fragments arranged by size:
3678 2046 1866 99 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4541 5717 6927 4541
Size: 1176 1210 5439
Fragments arranged by size:
5439 1210 1176
BclI T'GATC_A
Cuts at: 1548 3423 1548
Size: 1875 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2989 3676 4096
Size: 107 122 71 631 605 1317 687 420
Cuts at: 4096 6436 136
Size: 2340 1525
Fragments arranged by size:
2340 1525 1317 687 631 605 420 122
107 71
BglII A'GATC_T
Cuts at: 7820 7820
Size: 7825
BlpI GC'TnA_GC
Cuts at: 2679 2865 2979 3158 3881 5242 2679
Size: 186 114 179 723 1361 5262
Fragments arranged by size:
5262 1361 723 186 179 114
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2912 3681 4082 4101 4398 4624 4971
Size: 570 552 769 401 19 297 226 347
Cuts at: 4971 5886 7042 1790
Size: 915 1156 2573
Fragments arranged by size:
2573 1156 915 769 570 552 401 347
297 226 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 2423 4092
Size: 416 53 83 399 24 928 78 1669
Cuts at: 4092 4303 4701 4913 5556 6514 442
Size: 211 398 212 643 958 1753
Fragments arranged by size:
1753 1669 958 928 643 416 399 398
212 211 83 78 53 24
BmtI G_CTAG'C
Cuts at: 1056 3236 1056
Size: 2180 5645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 2270 2801 3379 3393 3693 3907 4087
Size: 951 80 531 578 14 300 214 180
Cuts at: 4087 5138 6485 1239
Size: 1051 1347 2579
Fragments arranged by size:
2579 1347 1051 951 578 531 300 214
180 80 14
Bpu10I CC'TnA_GC
Cuts at: 4428 4428
Size: 7825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4759 5950 6815 7113 3535
Size: 1224 1191 865 298 4247
Fragments arranged by size:
4247 1224 1191 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2418 2741 3698 4480 6488 882
Size: 617 919 323 957 782 2008 2219
Fragments arranged by size:
2219 2008 957 919 782 617 323
BsaAI yAC'GTr
Cuts at: 493 1204 3762 5616 493
Size: 711 2558 1854 2702
Fragments arranged by size:
2702 2558 1854 711
BsaBI GATnn'nnATC
Cuts at: 3314 4913 5099 7819 3314
Size: 1599 186 2720 3320
Fragments arranged by size:
3320 2720 1599 186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 3280 4199 4299
Size: 53 83 186 936 1015 732 919 100
Cuts at: 4299 5632 6014 6649 275
Size: 1333 382 635 1451
Fragments arranged by size:
1451 1333 1015 936 919 732 635 382
186 100 83 53
BsaWI w'CCGG_w
Cuts at: 3684 4113 6258 6656 6930 7077 7488 3684
Size: 429 2145 398 274 147 411 4021
Fragments arranged by size:
4021 2145 429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 3011 3041 3391
Size: 30 165 30 756 30 822 30 350
Cuts at: 3391 3421 3831 3861 1178
Size: 30 410 30 5142
Fragments arranged by size:
5142 822 756 410 350 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2168 2180 2432 2501 4176 1186
Size: 870 112 12 252 69 1675 4835
Fragments arranged by size:
4835 1675 870 252 112 69 12
BseYI C'CCAG_C
Cuts at: 2448 2720 2740 2885 3134 3735 4021 4565 5053
Size: 272 20 145 249 601 286 544 488
Cuts at: 5053 7028 7524 2448
Size: 1975 496 2749
Fragments arranged by size:
2749 1975 601 544 496 488 286 272
249 145 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2975 2993 3069 3996 4395 4532 1163
Size: 573 1239 18 76 927 399 137 4456
Fragments arranged by size:
4456 1239 927 573 399 137 76 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5275 6036 7064 1540
Size: 275 186 3274 761 1028 2301
Fragments arranged by size:
3274 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3357 4185 5124 5886 5971 7042 3357
Size: 828 939 762 85 1071 4140
Fragments arranged by size:
4140 1071 939 828 762 85
BsmI GAATG_Cn'
Cuts at: 5336 5429 5336
Size: 93 7732
BsmBI CGTCTCn'nnnn_
Cuts at: 4221 4221
Size: 7825
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2871 2912 3357 3681 4082 4101
Size: 399 570 511 41 445 324 401 19
Cuts at: 4101 4174 4185 4398 4440 4624 4971 5124 5144
Size: 73 11 213 42 184 347 153 20
Cuts at: 5144 5886 5971 7042 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 511 445 401 399
347 324 213 184 153 85 73 42
41 20 19 11
BspEI T'CCGG_A
Cuts at: 4113 7488 4113
Size: 3375 4450
BspHI T'CATG_A
Cuts at: 2295 2440 5714 7444 2295
Size: 145 3274 1730 2676
Fragments arranged by size:
3274 2676 1730 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2161 2485 2761 3042 3150 3473 3958
Size: 1199 118 324 276 281 108 323 485
Cuts at: 3958 4251 5180 844
Size: 293 929 3489
Fragments arranged by size:
3489 1199 929 485 324 323 293 281
276 118 108
BsrBI CCG'CTC
Cuts at: 1394 1823 5712 1394
Size: 429 3889 3507
Fragments arranged by size:
3889 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2583 4006 6320 6494 65
Size: 1183 1335 1423 2314 174 1396
Fragments arranged by size:
2314 1423 1396 1335 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3056 3441 4838 4895 6469 6656 1700
Size: 110 1246 385 1397 57 1574 187 2869
Fragments arranged by size:
2869 1574 1397 1246 385 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 2580 96
Size: 2484 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7825
BssSI C'ACGA_G
Cuts at: 4415 5885 6897 4415
Size: 1470 1012 5343
Fragments arranged by size:
5343 1470 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 2318 794
Size: 1382 142 6301
Fragments arranged by size:
6301 1382 142
BstBI TT'CG_AA
Cuts at: 6629 6629
Size: 7825
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 6236
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2641 3811 2353
Size: 288 1170 6367
Fragments arranged by size:
6367 1170 288
BstYI r'GATC_y
Cuts at: 1972 2093 2880 3309 3715 4365 4548 4983 5155
Size: 121 787 429 406 650 183 435 172
Cuts at: 5155 5522 5907 5924 7376 7462 7474 7820 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 787 650 435 429 406 385
367 346 183 172 121 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4147 4147
Size: 7825
BtgI C'CryG_G
Cuts at: 513 1065 2004 2160 3291 3298 3348 3615 3745
Size: 552 939 156 1131 7 50 267 130
Cuts at: 3745 3892 4384 4739 5088 513
Size: 147 492 355 349 3250
Fragments arranged by size:
3250 1131 939 552 492 355 349 267
156 147 130 50 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3901 5108 487
Size: 683 56 2675 1207 3204
Fragments arranged by size:
3204 2675 1207 683 56
BtsI GCAGTG_nn'
Cuts at: 2858 4407 5341 6135 6155 2858
Size: 1549 934 794 20 4528
Fragments arranged by size:
4528 1549 934 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7825
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3330 3365 529
Size: 35 2766 35 4989
Fragments arranged by size:
4989 2766 35 35
DraI TTT'AAA
Cuts at: 5129 5129
Size: 7825
DraIII CAC_nnn'GTG
Cuts at: 2557 5005 5089 2557
Size: 2448 84 5293
Fragments arranged by size:
5293 2448 84
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4204 4808 6832 809
Size: 507 2888 604 2024 1802
Fragments arranged by size:
2888 2024 1802 604 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 4936 5272
Size: 54 1425 50 275 18 798 2308 336
Cuts at: 5272 6161 7790 8
Size: 889 1629 43
Fragments arranged by size:
2308 1629 1425 889 798 336 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5272 1537
Size: 275 3460 4090
Fragments arranged by size:
4090 3460 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2074 4428 5755 1353
Size: 721 2354 1327 3423
Fragments arranged by size:
3423 2354 1327 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2942 3813 5039 6412 6786 6932
Size: 1064 138 1353 871 1226 1373 374 146
Cuts at: 6932 387
Size: 1280
Fragments arranged by size:
1373 1353 1280 1226 1064 871 374 146
138
EcoICRI GAG'CTC
Cuts at: 4183 5122 4183
Size: 939 6886
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2190 2270 2650 2718 2801 3244
Size: 393 423 135 80 380 68 83 443
Cuts at: 3244 3379 3393 3693 3907 4087 4947 5138 5888
Size: 135 14 300 214 180 860 191 750
Cuts at: 5888 6485 7272 7540 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 860 787 750 597 443 423 393
380 300 268 214 191 180 135 135
83 80 68 14
EcoNI CCTnn'n_nnAGG
Cuts at: 4255 4255
Size: 7825
EcoO109I rG'GnC_Cy
Cuts at: 3139 3830 4098 4671 4801 4967 3139
Size: 691 268 573 130 166 5997
Fragments arranged by size:
5997 691 573 268 166 130
EcoRI G'AATT_C
Cuts at: 2405 5134 5512 2405
Size: 2729 378 4718
Fragments arranged by size:
4718 2729 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 7825
FspI TGC'GCA
Cuts at: 6331 6331
Size: 7825
HaeII r_GCGC'y
Cuts at: 1678 3228 4302 6972 1678
Size: 1550 1074 2670 2531
Fragments arranged by size:
2670 2531 1550 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3011 3043 3391 3423 3776
Size: 32 135 32 1439 32 348 32 353
Cuts at: 3776 3808 4170 4202 6466 6498 6540 6572 1373
Size: 32 362 32 2264 32 42 32 2626
Fragments arranged by size:
2626 2264 1439 362 353 348 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3385 4192 5169 5415 669
Size: 988 281 1447 807 977 246 3079
Fragments arranged by size:
3079 1447 988 977 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7825
HpaI GTT'AAC
Cuts at: 5415 5415
Size: 7825
KasI G'GCGC_C
Cuts at: 4298 4298
Size: 7825
KpnI G_GTAC'C
Cuts at: 2238 3213 5150 2238
Size: 975 1937 4913
Fragments arranged by size:
4913 1937 975
MfeI C'AATT_G
Cuts at: 5424 5424
Size: 7825
MluI A'CGCG_T
Cuts at: 7701 7701
Size: 7825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 3054 6939 7123 1305
Size: 1065 684 3885 184 2007
Fragments arranged by size:
3885 2007 1065 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 4938 10
Size: 54 1425 343 798 2308 2897
Fragments arranged by size:
2897 2308 1425 798 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3764 4724 5024 5030 5783 6142
Size: 648 1803 795 960 300 6 753 359
Cuts at: 6142 6301 518
Size: 159 2042
Fragments arranged by size:
2042 1803 960 795 753 648 359 300
159 6
MspA1I CmG'CkG
Cuts at: 1606 2272 2505 2864 3293 3300 3739 3885 4158
Size: 666 233 359 429 7 439 146 273
Cuts at: 4158 4386 4427 4583 4632 4741 5241 5918 7066
Size: 228 41 156 49 109 500 677 1148
Cuts at: 7066 7311 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 666 500 439 429 359
273 245 233 228 156 146 109 49
41 7
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 7825
NarI GG'CG_CC
Cuts at: 4299 4299
Size: 7825
NcoI C'CATG_G
Cuts at: 513 1065 2004 3615 3745 513
Size: 552 939 1611 130 4593
Fragments arranged by size:
4593 1611 939 552 130
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7825
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 7825
NheI G'CTAG_C
Cuts at: 1052 3232 1052
Size: 2180 5645
NotI GC'GGCC_GC
Cuts at: 5272 5272
Size: 7825
NspI r_CATG'y
Cuts at: 1132 1229 2187 3181 5183 6728 1132
Size: 97 958 994 2002 1545 2229
Fragments arranged by size:
2229 2002 1545 994 958 97
PasI CC'CwG_GG
Cuts at: 1348 2089 4440 1348
Size: 741 2351 4733
Fragments arranged by size:
4733 2351 741
PciI A'CATG_T
Cuts at: 6724 6724
Size: 7825
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 2338 3190 3778 4461 4945 5066 5089
Size: 1167 63 852 588 683 484 121 23
Cuts at: 5089 1108
Size: 3844
Fragments arranged by size:
3844 1167 852 683 588 484 121 63
23
PfoI T'CCnGG_A
Cuts at: 4763 4763
Size: 7825
PmeI GTTT'AAAC
Cuts at: 5129 5129
Size: 7825
PmlI CAC'GTG
Cuts at: 3762 3762
Size: 7825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5894 5926 7407 7439 5894
Size: 32 1481 32 6280
Fragments arranged by size:
6280 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3139 3830 4801 3139
Size: 691 971 6163
Fragments arranged by size:
6163 971 691
PshAI GACnn'nnGTC
Cuts at: 1613 2547 1613
Size: 934 6891
PsiI TTA'TAA
Cuts at: 5395 5395
Size: 7825
PspOMI G'GGCC_C
Cuts at: 4097 4394 4967 4097
Size: 297 573 6955
Fragments arranged by size:
6955 573 297
PstI C_TGCA'G
Cuts at: 830 2403 2880 3955 4834 4993 5177 830
Size: 1573 477 1075 879 159 184 3478
Fragments arranged by size:
3478 1573 1075 879 477 184 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7825
PvuII CAG'CTG
Cuts at: 2272 2864 3739 3885 4427 4583 2272
Size: 592 875 146 542 156 5514
Fragments arranged by size:
5514 875 592 542 156 146
RsrII CG'GwC_CG
Cuts at: 4588 4588
Size: 7825
SacI G_AGCT'C
Cuts at: 4185 5124 4185
Size: 939 6886
SacII CC_GC'GG
Cuts at: 3294 3301 4387 4742 3294
Size: 7 1086 355 6377
Fragments arranged by size:
6377 1086 355 7
SalI G'TCGA_C
Cuts at: 1936 5167 1936
Size: 3231 4594
SanDI GG'GwC_CC
Cuts at: 4801 4801
Size: 7825
SapI GCTCTTCn'nnn_
Cuts at: 4428 4428
Size: 7825
SbfI CC_TGCA'GG
Cuts at: 3955 5177 3955
Size: 1222 6603
ScaI AGT'ACT
Cuts at: 4313 6073 7785 4313
Size: 1760 1712 4353
Fragments arranged by size:
4353 1760 1712
SexAI A'CCwGG_T
Cuts at: 2286 2286
Size: 7825
SfcI C'TryA_G
Cuts at: 826 1045 2399 2876 3072 3587 3951 4321 4830
Size: 219 1354 477 196 515 364 370 509
Cuts at: 4830 4989 5173 6308 6989 7180 826
Size: 159 184 1135 681 191 1471
Fragments arranged by size:
1471 1354 1135 681 515 509 477 370
364 219 196 191 184 159
SfoI GGC'GCC
Cuts at: 4300 4300
Size: 7825
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7825
SmaI CCC'GGG
Cuts at: 1872 2195 3332 4289 5152 1872
Size: 323 1137 957 863 4545
Fragments arranged by size:
4545 1137 957 863 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3550 4774 5929 6830 7092 820
Size: 197 926 1607 1224 1155 901 262 1553
Fragments arranged by size:
1607 1553 1224 1155 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5616 493
Size: 5123 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7825
SphI G_CATG'C
Cuts at: 1132 2187 5183 1132
Size: 1055 2996 3774
Fragments arranged by size:
3774 2996 1055
SrfI GCCC'GGGC
Cuts at: 2195 2195
Size: 7825
SspI AAT'ATT
Cuts at: 5 52 5749 5
Size: 47 5697 2081
Fragments arranged by size:
5697 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7825
StyI C'CwwG_G
Cuts at: 513 1065 2004 2307 2412 2937 3615 3745 3795
Size: 552 939 303 105 525 678 130 50
Cuts at: 3795 4455 5264 513
Size: 660 809 3074
Fragments arranged by size:
3074 939 809 678 660 552 525 303
130 105 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2734 3560 4605 5852 5869 6022 6207
Size: 1255 1154 826 1045 1247 17 153 185
Cuts at: 6207 6652 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1247 1154 1045 826 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3756 3924
Size: 274 80 33 51 1443 603 1176 168
Cuts at: 3924 4311 4419 6071 7783 96
Size: 387 108 1652 1712 138
Fragments arranged by size:
1712 1652 1443 1176 603 387 274 168
138 108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 3831 3863 7785 7817 2543
Size: 32 1256 32 3922 32 2551
Fragments arranged by size:
3922 2551 1256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7825
XbaI T'CTAG_A
Cuts at: 5161 5161
Size: 7825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 3160 3743 4072 2638
Size: 522 583 329 6391
Fragments arranged by size:
6391 583 522 329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7825
XmaI C'CCGG_G
Cuts at: 1870 2193 3330 4287 5150 1870
Size: 323 1137 957 863 4545
Fragments arranged by size:
4545 1137 957 863 323
XmnI GAAnn'nnTTC
Cuts at: 5954 5954
Size: 7825
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5633 276
Size: 53 83 186 936 4099 2468
Fragments arranged by size:
4099 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PspXI PsrI SfiI
SwaI