Restriction map for
pFN21AE2504
(Circular) (Six-base) MAPSORT of: pFN21AE2504.seq Check: 6803 from: 1 to: 7747
pFN21AE2504 7747 bp
With 182 enzymes: SgfI *
February 16, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2949 3390 5557 278
Size: 53 83 186 936 1413 441 2167 2468
Fragments arranged by size:
2468 2167 1413 936 441 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4905 5090 1937
Size: 2968 185 4594
Fragments arranged by size:
4594 2968 185
Acc65I G'GTAC_C
Cuts at: 2157 2475 5068 2157
Size: 318 2593 4836
Fragments arranged by size:
4836 2593 318
AclI AA'CG_TT
Cuts at: 1563 5874 6247 1563
Size: 4311 373 3063
Fragments arranged by size:
4311 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2539 2609 2807 3141 3395 3646 4067 4449
Size: 907 70 198 334 254 251 421 382
Cuts at: 4449 4671 4820 4863 5021 5810 7194 7462 1632
Size: 222 149 43 158 789 1384 268 1917
Fragments arranged by size:
1917 1384 907 789 421 382 334 268
254 251 222 198 158 149 70 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7550
AflIII A'CryG_T
Cuts at: 1203 4671 4788 6646 7623 1203
Size: 3468 117 1858 977 1327
Fragments arranged by size:
3468 1858 1327 977 117
AgeI A'CCGG_T
Cuts at: 6578 6578
Size: 7747
AhdI GACnn_n'nnGTC
Cuts at: 6476 6476
Size: 7747
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7747
AlwNI CAG_nnn'CTG
Cuts at: 3470 3506 3727 3768 4192 5490 7062 3470
Size: 36 221 41 424 1298 1572 4155
Fragments arranged by size:
4155 1572 1298 424 221 41 36
ApaLI G'TGCA_C
Cuts at: 5804 6960 5804
Size: 1156 6591
ApoI r'AATT_y
Cuts at: 1493 3017 3892 5021 5056 5282 5434 7543 1493
Size: 1524 875 1129 35 226 152 2109 1697
Fragments arranged by size:
2109 1697 1524 1129 875 226 152 35
AscI GG'CGCG_CC
Cuts at: 2056 2056
Size: 7747
AseI AT'TA_AT
Cuts at: 160 1032 6301 160
Size: 872 5269 1606
Fragments arranged by size:
5269 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7747
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 2899 3197 3248 5072 1870
Size: 73 582 374 298 51 1824 4545
Fragments arranged by size:
4545 1824 582 374 298 73 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2141 2174 2141
Size: 33 7714
BamHI G'GATC_C
Cuts at: 3604 4366 4617 5077 5444 3604
Size: 762 251 460 367 5907
Fragments arranged by size:
5907 762 460 367 251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2157 2199 2475 2873 3983
Size: 325 217 627 370 42 276 398 1110
Cuts at: 3983 5068 6523 618
Size: 1085 1455 1842
Fragments arranged by size:
1842 1455 1110 1085 627 398 370 325
276 217 42
BanII G_rGCy'C
Cuts at: 1391 5066 1391
Size: 3675 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2007 2270 2333 2905 3652 4625 928
Size: 971 108 263 63 572 747 973 4050
Fragments arranged by size:
4050 973 971 747 572 263 108 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2516 2550 2851 2885 5938 5972 1903
Size: 34 579 34 301 34 3053 34 3678
Fragments arranged by size:
3678 3053 579 301 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3915 5639 6849 3915
Size: 1724 1210 4813
Fragments arranged by size:
4813 1724 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2720 3098 3278
Size: 107 122 71 631 605 1048 378 180
Cuts at: 3278 6358 136
Size: 3080 1525
Fragments arranged by size:
3080 1525 1048 631 605 378 180 122
107 71
BglII A'GATC_T
Cuts at: 7742 7742
Size: 7747
BlpI GC'TnA_GC
Cuts at: 4106 5164 4106
Size: 1058 6689
Bme1580I G_kGCm'C
Cuts at: 1790 2878 5808 6964 1790
Size: 1088 2930 1156 2573
Fragments arranged by size:
2930 2573 1156 1088
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7747
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2949 5478 6436
Size: 416 53 83 399 24 1532 2529 958
Cuts at: 6436 442
Size: 1753
Fragments arranged by size:
2529 1753 1532 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7747
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 7747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 3365 3662 3749 3947 6407 1239
Size: 1059 1067 297 87 198 2460 2579
Fragments arranged by size:
2579 2460 1067 1059 297 198 87
Bpu10I CC'TnA_GC
Cuts at: 4337 4989 4337
Size: 652 7095
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 3023 5872 6737 7035 2531
Size: 492 2849 865 298 3243
Fragments arranged by size:
3243 2849 865 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4711 4737 6410 882
Size: 617 3212 26 1673 2219
Fragments arranged by size:
3212 2219 1673 617 26
BsaAI yAC'GTr
Cuts at: 493 1204 2376 5538 493
Size: 711 1172 3162 2702
Fragments arranged by size:
3162 2702 1172 711
BsaBI GATnn'nnATC
Cuts at: 2111 7741 2111
Size: 5630 2117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2946 3387 5554 5936
Size: 53 83 186 936 1413 441 2167 382
Cuts at: 5936 6571 275
Size: 635 1451
Fragments arranged by size:
2167 1451 1413 936 635 441 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2103 2991 6180 6578 6852 6999 7410 2103
Size: 888 3189 398 274 147 411 2440
Fragments arranged by size:
3189 2440 888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 3585 3615 4691
Size: 30 165 30 747 30 1405 30 1076
Cuts at: 4691 4721 1178
Size: 30 4204
Fragments arranged by size:
4204 1405 1076 747 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2295 2522 3139 3606 1186
Size: 1109 227 617 467 5327
Fragments arranged by size:
5327 1109 617 467 227
BseYI C'CCAG_C
Cuts at: 2512 4613 6950 7446 2512
Size: 2101 2337 496 2813
Fragments arranged by size:
2813 2337 2101 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 2920 4298 1163
Size: 573 405 779 1378 4612
Fragments arranged by size:
4612 1378 779 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5197 5958 6986 1540
Size: 275 186 3196 761 1028 2301
Fragments arranged by size:
3196 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 5808 5893 6964 3843
Size: 1965 85 1071 4626
Fragments arranged by size:
4626 1965 1071 85
BsmI GAATG_Cn'
Cuts at: 2900 4541 5258 5351 2900
Size: 1641 717 93 5296
Fragments arranged by size:
5296 1641 717 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3394 4740 3394
Size: 1346 6401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2878 3843 5066 5808 5893 6964 1391
Size: 399 1088 965 1223 742 85 1071 2174
Fragments arranged by size:
2174 1223 1088 1071 965 742 399 85
BspEI T'CCGG_A
Cuts at: 2103 7410 2103
Size: 5307 2440
BspHI T'CATG_A
Cuts at: 3846 5636 7366 3846
Size: 1790 1730 4227
Fragments arranged by size:
4227 1790 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4314 5102 844
Size: 3470 788 3489
Fragments arranged by size:
3489 3470 788
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 4255 5634 1394
Size: 429 229 2203 1379 3507
Fragments arranged by size:
3507 2203 1379 429 229
BsrDI GCAATG_nn'
Cuts at: 65 1248 6242 6416 65
Size: 1183 4994 174 1396
Fragments arranged by size:
4994 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6391 6578 1700
Size: 110 4581 187 2869
Fragments arranged by size:
4581 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7747
BssHII G'CGCG_C
Cuts at: 1925 2056 1925
Size: 131 7616
BssSI C'ACGA_G
Cuts at: 5807 6819 5807
Size: 1012 6735
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2073 2354 2818 794
Size: 1279 281 464 5723
Fragments arranged by size:
5723 1279 464 281
BstBI TT'CG_AA
Cuts at: 6551 6551
Size: 7747
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 5927
BstXI CCAn_nnnn'nTGG
Cuts at: 2968 4235 2968
Size: 1267 6480
BstYI r'GATC_y
Cuts at: 1972 3186 3604 4119 4188 4195 4366 4617 4686
Size: 1214 418 515 69 7 171 251 69
Cuts at: 4686 5077 5444 5829 5846 7298 7384 7396 7742
Size: 391 367 385 17 1452 86 12 346
Cuts at: 7742 1972
Size: 1977
Fragments arranged by size:
1977 1452 1214 515 418 391 385 367
346 251 171 86 69 69 17 12
7
BtgI C'CryG_G
Cuts at: 513 1065 2004 3096 4059 513
Size: 552 939 1092 963 4201
Fragments arranged by size:
4201 1092 963 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2649 2937 487
Size: 683 56 1423 288 5297
Fragments arranged by size:
5297 1423 683 288 56
BtsI GCAGTG_nn'
Cuts at: 2949 3963 5263 6057 6077 2949
Size: 1014 1300 794 20 4619
Fragments arranged by size:
4619 1300 1014 794 20
ClaI AT'CG_AT
Cuts at: 1554 4374 4506 1554
Size: 2820 132 4795
Fragments arranged by size:
4795 2820 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4591 4626 529
Size: 35 4027 35 3650
Fragments arranged by size:
4027 3650 35 35
DraI TTT'AAA
Cuts at: 5051 5051
Size: 7747
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4499 6754 809
Size: 507 3183 2255 1802
Fragments arranged by size:
3183 2255 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 3102 3270
Size: 54 1425 50 275 18 279 993 168
Cuts at: 3270 3940 5194 6083 7712 8
Size: 670 1254 889 1629 43
Fragments arranged by size:
1629 1425 1254 993 889 670 279 275
168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5194 1537
Size: 275 3382 4090
Fragments arranged by size:
4090 3382 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3945 5677 1353
Size: 2592 1732 3423
Fragments arranged by size:
3423 2592 1732
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3102 3382 6334 6708 6854
Size: 1064 138 435 1078 280 2952 374 146
Cuts at: 6854 387
Size: 1280
Fragments arranged by size:
2952 1280 1078 1064 435 374 280 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2298 2539 2609 2807 3141 3365 3395
Size: 393 666 241 70 198 334 224 30
Cuts at: 3395 3646 3662 3749 3947 4067 4449 4671 4820
Size: 251 16 87 198 120 382 222 149
Cuts at: 4820 4863 5021 5810 6407 7194 7462 1239
Size: 43 158 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 666 597 393 382 334
268 251 241 224 222 198 198 158
149 120 87 70 43 30 16
EcoNI CCTnn'n_nnAGG
Cuts at: 4145 4625 4145
Size: 480 7267
EcoO109I rG'GnC_Cy
Cuts at: 3765 4015 3765
Size: 250 7497
EcoRI G'AATT_C
Cuts at: 3017 5056 5434 3017
Size: 2039 378 5330
Fragments arranged by size:
5330 2039 378
EcoRV GAT'ATC
Cuts at: 4510 4510
Size: 7747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4048 4111 4796 4048
Size: 63 685 6999
Fragments arranged by size:
6999 685 63
FspI TGC'GCA
Cuts at: 3633 6253 3633
Size: 2620 5127
HaeII r_GCGC'y
Cuts at: 1678 3093 4658 6894 1678
Size: 1415 1565 2236 2531
Fragments arranged by size:
2531 2236 1565 1415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 3178 3210 3961
Size: 32 135 32 638 32 936 32 751
Cuts at: 3961 3993 4180 4212 4691 4723 6388 6420 6462
Size: 32 187 32 479 32 1665 32 42
Cuts at: 6462 6494 1373
Size: 32 2626
Fragments arranged by size:
2626 1665 936 751 638 479 187 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 4381 4417 5091 5337 669
Size: 988 281 1323 1120 36 674 246 3079
Fragments arranged by size:
3079 1323 1120 988 674 281 246 36
HindIII A'AGCT_T
Cuts at: 748 2702 748
Size: 1954 5793
HpaI GTT'AAC
Cuts at: 5337 5337
Size: 7747
KpnI G_GTAC'C
Cuts at: 2161 2479 5072 2161
Size: 318 2593 4836
Fragments arranged by size:
4836 2593 318
MfeI C'AATT_G
Cuts at: 5346 5346
Size: 7747
MluI A'CGCG_T
Cuts at: 7623 7623
Size: 7747
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 3201 6861 7045 1305
Size: 1083 813 3660 184 2007
Fragments arranged by size:
3660 2007 1083 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 3942 10
Size: 54 1425 343 279 1831 3815
Fragments arranged by size:
3815 1831 1425 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 4025 4319 4409 5705 6064 6223
Size: 648 951 1908 294 90 1296 359 159
Cuts at: 6223 518
Size: 2042
Fragments arranged by size:
2042 1908 1296 951 648 359 294 159
90
MspA1I CmG'CkG
Cuts at: 1606 2270 2472 2908 3599 5163 5840 6988 7233
Size: 664 202 436 691 1564 677 1148 245
Cuts at: 7233 1606
Size: 2120
Fragments arranged by size:
2120 1564 1148 691 677 664 436 245
202
NcoI C'CATG_G
Cuts at: 513 1065 2004 4059 513
Size: 552 939 2055 4201
Fragments arranged by size:
4201 2055 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7747
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7747
NotI GC'GGCC_GC
Cuts at: 5194 5194
Size: 7747
NspI r_CATG'y
Cuts at: 1132 1229 3638 4675 4792 5105 6650 1132
Size: 97 2409 1037 117 313 1545 2229
Fragments arranged by size:
2409 2229 1545 1037 313 117 97
PasI CC'CwG_GG
Cuts at: 1348 4627 1348
Size: 3279 4468
PciI A'CATG_T
Cuts at: 4671 4788 6646 4671
Size: 117 1858 5772
Fragments arranged by size:
5772 1858 117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7747
PfoI T'CCnGG_A
Cuts at: 3209 3381 3639 4191 4595 3209
Size: 172 258 552 404 6361
Fragments arranged by size:
6361 552 404 258 172
PmeI GTTT'AAAC
Cuts at: 5051 5051
Size: 7747
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5816 5848 7329 7361 5816
Size: 32 1481 32 6202
Fragments arranged by size:
6202 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3765 4015 3765
Size: 250 7497
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7747
PsiI TTA'TAA
Cuts at: 5317 5317
Size: 7747
PstI C_TGCA'G
Cuts at: 830 3723 5099 830
Size: 2893 1376 3478
Fragments arranged by size:
3478 2893 1376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7747
PvuII CAG'CTG
Cuts at: 2908 3599 2908
Size: 691 7056
SalI G'TCGA_C
Cuts at: 1936 5089 1936
Size: 3153 4594
SbfI CC_TGCA'GG
Cuts at: 5099 5099
Size: 7747
ScaI AGT'ACT
Cuts at: 5995 7707 5995
Size: 1712 6035
SfcI C'TryA_G
Cuts at: 826 1045 2568 3719 4327 5095 6230 6911 7102
Size: 219 1523 1151 608 768 1135 681 191
Cuts at: 7102 826
Size: 1471
Fragments arranged by size:
1523 1471 1151 1135 768 681 608 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7747
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7747
SmaI CCC'GGG
Cuts at: 1872 5074 1872
Size: 3202 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 3038 5851 6752 7014 820
Size: 197 926 603 492 2813 901 262 1553
Fragments arranged by size:
2813 1553 926 901 603 492 262 197
SnaBI TAC'GTA
Cuts at: 493 5538 493
Size: 5045 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7747
SphI G_CATG'C
Cuts at: 1132 5105 1132
Size: 3973 3774
SspI AAT'ATT
Cuts at: 5 52 5671 5
Size: 47 5619 2081
Fragments arranged by size:
5619 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7747
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 3443 3673 3822 4059 4095
Size: 552 939 87 1352 230 149 237 36
Cuts at: 4095 4284 4780 4830 5186 513
Size: 189 496 50 356 3074
Fragments arranged by size:
3074 1352 939 552 496 356 237 230
189 149 87 50 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2324 2862 3001 5774 5791 5944 6129
Size: 1255 744 538 139 2773 17 153 185
Cuts at: 6129 6574 325
Size: 445 1498
Fragments arranged by size:
2773 1498 1255 744 538 445 185 153
139 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 4968 5993
Size: 274 80 33 51 1443 552 2439 1025
Cuts at: 5993 7705 96
Size: 1712 138
Fragments arranged by size:
2439 1712 1443 1025 552 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3585 3617 3889 3921 7707 7739 3422
Size: 32 131 32 272 32 3786 32 3430
Fragments arranged by size:
3786 3430 272 131 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4703 1316
Size: 3387 4360
XbaI T'CTAG_A
Cuts at: 5083 5083
Size: 7747
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 3094 3307 3676 2306
Size: 788 213 369 6377
Fragments arranged by size:
6377 788 369 213
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7747
XmaI C'CCGG_G
Cuts at: 1870 5072 1870
Size: 3202 4545
XmnI GAAnn'nnTTC
Cuts at: 3659 5876 3659
Size: 2217 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2947 3388 5555 276
Size: 53 83 186 936 1413 441 2167 2468
Fragments arranged by size:
2468 2167 1413 936 441 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI