Restriction map for
pFN21AE2503
(Circular) (Six-base) MAPSORT of: pFN21AE2503.seq Check: 3702 from: 1 to: 6598
pFN21AE2503 6598 bp
With 182 enzymes: SgfI *
February 16, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2347 3941 1937
Size: 410 1594 4594
Fragments arranged by size:
4594 1594 410
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 2578 4725 5098 1563
Size: 1015 2147 373 3063
Fragments arranged by size:
3063 2147 1015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 3098 3140 3215 4661 6045 6313 1632
Size: 485 981 42 75 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 981 485 268 75 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 2731 5497 6474 1203
Size: 1528 2766 977 1327
Fragments arranged by size:
2766 1528 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 6566
AlwNI CAG_nnn'CTG
Cuts at: 2051 2719 3163 3326 3849 4341 5913 2051
Size: 668 444 163 523 492 1572 2736
Fragments arranged by size:
2736 1572 668 523 492 444 163
ApaI G_GGCC'C
Cuts at: 2815 2815
Size: 6598
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 3608 3907 4133 4285 6394 1493
Size: 2115 299 226 152 2109 1697
Fragments arranged by size:
2115 2109 1697 299 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 2326 2659 3674 3923 1870
Size: 73 383 333 1015 249 4545
Fragments arranged by size:
4545 1015 383 333 249 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 3919 5374 618
Size: 325 217 627 396 1736 1455 1842
Fragments arranged by size:
1842 1736 1455 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2297 2815 3030 3658 3675 3917 1391
Size: 654 252 518 215 628 17 242 4072
Fragments arranged by size:
4072 654 628 518 252 242 215 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4490 5700 4490
Size: 1210 5388
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5209 136
Size: 107 122 71 631 605 3537 1525
Fragments arranged by size:
3537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 2940 4015 2940
Size: 1075 5523
Bme1580I G_kGCm'C
Cuts at: 1790 2188 2815 3603 4659 5815 1790
Size: 398 627 788 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 788 627 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 2809 3336
Size: 416 53 83 399 24 772 620 527
Cuts at: 3336 3639 4329 5287 442
Size: 303 690 958 1753
Fragments arranged by size:
1753 958 772 690 620 527 416 399
303 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2792 2924 3014 3029 3380 3573 5258
Size: 887 666 132 90 15 351 193 1685
Cuts at: 5258 1239
Size: 2579
Fragments arranged by size:
2579 1685 887 666 351 193 132 90
15
Bpu10I CC'TnA_GC
Cuts at: 3036 3036
Size: 6598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 4723 5588 5886 2666
Size: 2057 865 298 3378
Fragments arranged by size:
3378 2057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2616 5261 882
Size: 617 1117 2645 2219
Fragments arranged by size:
2645 2219 1117 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 4389 493
Size: 711 1530 1655 2702
Fragments arranged by size:
2702 1655 1530 711
BsaBI GATnn'nnATC
Cuts at: 2886 6592 2886
Size: 3706 2892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3420 5031 5429 5703 5850 6261 3420
Size: 1611 398 274 147 411 3757
Fragments arranged by size:
3757 1611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2119 2149 3555 3585 1178
Size: 30 165 30 716 30 1406 30 4191
Fragments arranged by size:
4191 1406 716 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2251 2260 2263 2882 2912 2923 3119
Size: 967 98 9 3 619 30 11 196
Cuts at: 3119 3203 3377 3513 3546 1186
Size: 84 174 136 33 4238
Fragments arranged by size:
4238 967 619 196 174 136 98 84
33 30 11 9 3
BseYI C'CCAG_C
Cuts at: 2219 2962 3256 3313 3658 3870 5801 6297 2219
Size: 743 294 57 345 212 1931 496 2520
Fragments arranged by size:
2520 1931 743 496 345 294 212 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2097 2152 3720 3765 3815 1163
Size: 573 361 55 1568 45 50 3946
Fragments arranged by size:
3946 1568 573 361 55 50 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3288 4048 4809 5837 1540
Size: 275 186 568 719 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 719 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 3030 3675 3831 4659 4744 5815 3030
Size: 645 156 828 85 1071 3813
Fragments arranged by size:
3813 1071 828 645 156 85
BsmI GAATG_Cn'
Cuts at: 4109 4202 4109
Size: 93 6505
BsmBI CGTCTCn'nnnn_
Cuts at: 3266 3266
Size: 6598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2188 2297 2815 3030 3603 3658
Size: 399 255 143 109 518 215 573 55
Cuts at: 3658 3675 3831 3917 4659 4744 5815 1391
Size: 17 156 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 518 399 255 215
156 143 109 86 85 55 17
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3953 844
Size: 3109 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5093 5267 65
Size: 1183 3845 174 1396
Fragments arranged by size:
3845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2564 5242 5429 1700
Size: 110 754 2678 187 2869
Fragments arranged by size:
2869 2678 754 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 3200 4658 5670 3200
Size: 1458 1012 4128
Fragments arranged by size:
4128 1458 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2719 3849 794
Size: 1925 1130 3543
Fragments arranged by size:
3543 1925 1130
BstBI TT'CG_AA
Cuts at: 5402 5402
Size: 6598
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 3727 2560
Size: 1167 5431
BstYI r'GATC_y
Cuts at: 1972 2101 2557 3928 4295 4680 4697 6149 6235
Size: 129 456 1371 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1371 456 385 367 346 129
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2996 3319 3377 2996
Size: 323 58 6217
Fragments arranged by size:
6217 323 58
BtgI C'CryG_G
Cuts at: 513 1065 3073 513
Size: 552 2008 4038
Fragments arranged by size:
4038 2008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2568 3434 487
Size: 683 56 1342 866 3651
Fragments arranged by size:
3651 1342 866 683 56
BtsI GCAGTG_nn'
Cuts at: 2140 2945 2973 3215 4114 4908 4928 2140
Size: 805 28 242 899 794 20 3810
Fragments arranged by size:
3810 899 805 794 242 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 2566 3165
Size: 54 1425 50 275 18 732 4 599
Cuts at: 3165 3285 3615 4045 4934 6563 8
Size: 120 330 430 889 1629 43
Fragments arranged by size:
1629 1425 889 732 599 430 330 275
120 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2566 3285 4045 1537
Size: 275 754 719 760 4090
Fragments arranged by size:
4090 760 754 719 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2541 3143 4528 1353
Size: 1188 602 1385 3423
Fragments arranged by size:
3423 1385 1188 602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2140 2254 3386 5185 5559 5705
Size: 1064 138 551 114 1132 1799 374 146
Cuts at: 5705 387
Size: 1280
Fragments arranged by size:
1799 1280 1132 1064 551 374 146 138
114
EcoICRI GAG'CTC
Cuts at: 3028 3673 3028
Size: 645 5953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2126 2792 2924 3014 3029 3098
Size: 393 485 9 666 132 90 15 69
Cuts at: 3098 3140 3215 3380 3573 4661 5258 6045 6313
Size: 42 75 165 193 1088 597 787 268
Cuts at: 6313 1239
Size: 1524
Fragments arranged by size:
1524 1088 787 666 597 485 393 268
193 165 132 90 75 69 42 15
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2999 3359 3738 2999
Size: 360 379 5859
Fragments arranged by size:
5859 379 360
EcoO109I rG'GnC_Cy
Cuts at: 2811 2811
Size: 6598
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FseI GG_CCGG'CC
Cuts at: 2568 2568
Size: 6598
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 5745 1678
Size: 4067 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2642 2674 3421
Size: 32 135 32 479 32 559 32 747
Cuts at: 3421 3453 5239 5271 5313 5345 1373
Size: 32 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 747 559 479 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2348 3942 4188 669
Size: 988 281 410 1594 246 3079
Fragments arranged by size:
3079 1594 988 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 2181 3204 5712 5896 1305
Size: 711 165 1023 2508 184 2007
Fragments arranged by size:
2508 2007 1023 711 184 165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4776
Fragments arranged by size:
4776 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2071 4556 4915 5074 518
Size: 648 905 2485 359 159 2042
Fragments arranged by size:
2485 2042 905 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 2198 2404 2770 3163 3620 4014 4691
Size: 567 25 206 366 393 457 394 677
Cuts at: 4691 5839 6084 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 457 394 393 366
245 206 25
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 6598
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6046
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NruI TCG'CGA
Cuts at: 3096 3096
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2285 3389 3956 5501 1132
Size: 97 1056 1104 567 1545 2229
Fragments arranged by size:
2229 1545 1104 1056 567 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2018 3268 1108
Size: 910 1250 4438
Fragments arranged by size:
4438 1250 910
PfoI T'CCnGG_A
Cuts at: 3357 3649 3357
Size: 292 6306
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2811 2811
Size: 6598
PspXI vC'TCGA_Gb
Cuts at: 2326 2326
Size: 6598
PstI C_TGCA'G
Cuts at: 830 3222 3249 3562 3950 830
Size: 2392 27 313 388 3478
Fragments arranged by size:
3478 2392 388 313 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2198 2404 2770 3163 2198
Size: 206 366 393 5633
Fragments arranged by size:
5633 393 366 206
SacI G_AGCT'C
Cuts at: 3030 3675 3030
Size: 645 5953
SalI G'TCGA_C
Cuts at: 1936 2346 3940 1936
Size: 410 1594 4594
Fragments arranged by size:
4594 1594 410
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 3218 3245 3558 3946 5081 5762 5953
Size: 219 2173 27 313 388 1135 681 191
Cuts at: 5953 826
Size: 1471
Fragments arranged by size:
2173 1471 1135 681 388 313 219 191
27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 2659 2681 4702 5603 5865
Size: 197 926 383 333 22 2021 901 262
Cuts at: 5865 820
Size: 1553
Fragments arranged by size:
2021 1553 926 901 383 333 262 197
22
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 2285 3956 1132
Size: 1153 1671 3774
Fragments arranged by size:
3774 1671 1153
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6598
StyI C'CwwG_G
Cuts at: 513 1065 2553 3802 4037 513
Size: 552 1488 1249 235 3074
Fragments arranged by size:
3074 1488 1249 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 3846 4625 4642 4795 4980 5425
Size: 1255 591 1675 779 17 153 185 445
Cuts at: 5425 325
Size: 1498
Fragments arranged by size:
1675 1498 1255 779 591 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4844 6556 96
Size: 274 80 33 51 1443 2867 1712 138
Fragments arranged by size:
2867 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2119 2151 6558 6590 2119
Size: 32 4407 32 2127
Fragments arranged by size:
4407 2127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3512 3867 3512
Size: 355 6243
XhoI C'TCGA_G
Cuts at: 1943 2326 2659 1943
Size: 383 333 5882
Fragments arranged by size:
5882 383 333
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI KasI
NarI NsiI PacI PpuMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI