Restriction map for
pFN21AE2498
(Circular) (Six-base) MAPSORT of: pFN21AE2498.seq Check: 9896 from: 1 to: 17869
pFN21AE2498 17869 bp
With 182 enzymes: SgfI *
March 20, 2014 17:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2890 6262 8623 14959 2890
Size: 3372 2361 6336 5800
Fragments arranged by size:
6336 5800 3372 2361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11775 15679 278
Size: 53 83 186 936 10239 3904 2468
Fragments arranged by size:
10239 3904 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 3766 5477 10569 15212 1937
Size: 1533 296 1711 5092 4643 4594
Fragments arranged by size:
5092 4643 4594 1711 1533 296
Acc65I G'GTAC_C
Cuts at: 4949 13658 14412 15190 4949
Size: 8709 754 778 7628
Fragments arranged by size:
8709 7628 778 754
AclI AA'CG_TT
Cuts at: 1563 3010 4563 5691 15996 16369 1563
Size: 1447 1553 1128 10305 373 3063
Fragments arranged by size:
10305 3063 1553 1447 1128 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 3426 4035 4064 4299 4584 5007 5288
Size: 554 1240 609 29 235 285 423 281
Cuts at: 5288 5437 5826 5882 6366 6869 7589 8217 8378
Size: 149 389 56 484 503 720 628 161
Cuts at: 8378 10064 10199 10257 10394 11875 12648 13251 14271
Size: 1686 135 58 137 1481 773 603 1020
Cuts at: 14271 15932 17316 17584 1632
Size: 1661 1384 268 1917
Fragments arranged by size:
1917 1686 1661 1481 1384 1240 1020 773
720 628 609 603 554 503 484 423
389 285 281 268 235 161 149 137
135 58 56 29
AfeI AGC'GCT
Cuts at: 8057 8057
Size: 17869
AflII C'TTAA_G
Cuts at: 820 1017 14030 820
Size: 197 13013 4659
Fragments arranged by size:
13013 4659 197
AflIII A'CryG_T
Cuts at: 1203 3465 4543 16768 17745 1203
Size: 2262 1078 12225 977 1327
Fragments arranged by size:
12225 2262 1327 1078 977
AgeI A'CCGG_T
Cuts at: 16700 16700
Size: 17869
AhdI GACnn_n'nnGTC
Cuts at: 10495 16598 10495
Size: 6103 11766
AleI CACnn'nnGTG
Cuts at: 1166 9488 10421 1166
Size: 8322 933 8614
Fragments arranged by size:
8614 8322 933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3305 9506 9813 1184
Size: 2121 6201 307 9240
Fragments arranged by size:
9240 6201 2121 307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 12271 12303 12603 12635 3688
Size: 32 8551 32 300 32 8922
Fragments arranged by size:
8922 8551 300 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4277 6744 9946 10088 10961 11918 13076 14719 14829
Size: 2467 3202 142 873 957 1158 1643 110
Cuts at: 14829 15612 17184 4277
Size: 783 1572 4962
Fragments arranged by size:
4962 3202 2467 1643 1572 1158 957 873
783 142 110
ApaI G_GGCC'C
Cuts at: 5125 6922 14712 5125
Size: 1797 7790 8282
Fragments arranged by size:
8282 7790 1797
ApaLI G'TGCA_C
Cuts at: 10696 11127 13580 13767 15926 17082 10696
Size: 431 2453 187 2159 1156 11483
Fragments arranged by size:
11483 2453 2159 1156 431 187
ApoI r'AATT_y
Cuts at: 1493 2457 2609 2867 3502 3634 4178 4801 4877
Size: 964 152 258 635 132 544 623 76
Cuts at: 4877 5214 5287 5722 5941 6229 6587 6962 7340
Size: 337 73 435 219 288 358 375 378
Cuts at: 7340 7397 8146 8644 8786 8820 9615 9637 10207
Size: 57 749 498 142 34 795 22 570
Cuts at: 10207 10859 11008 11698 12260 12775 14795 15178 15404
Size: 652 149 690 562 515 2020 383 226
Cuts at: 15404 15556 17665 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 2020 1697 964 795 749 690 652
635 623 570 562 544 515 498 435
383 378 375 358 337 288 258 226
219 152 152 149 142 132 76 73
57 34 22
AseI AT'TA_AT
Cuts at: 160 1032 4662 11613 13299 13530 14088 16423 160
Size: 872 3630 6951 1686 231 558 2335 1606
Fragments arranged by size:
6951 3630 2335 1686 1606 872 558 231
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17869
AvaI C'yCGr_G
Cuts at: 1870 1943 3587 4346 6051 7077 14106 15194 1870
Size: 73 1644 759 1705 1026 7029 1088 4545
Fragments arranged by size:
7029 4545 1705 1644 1088 1026 759 73
AvrII C'CTAG_G
Cuts at: 8347 8347
Size: 17869
BamHI G'GATC_C
Cuts at: 7609 9604 10534 11825 15199 15566 7609
Size: 1995 930 1291 3374 367 9912
Fragments arranged by size:
9912 3374 1995 1291 930 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4949 5603 8007 8198 8536
Size: 325 217 627 3162 654 2404 191 338
Cuts at: 8536 9390 9482 13658 14412 14600 15190 16645 618
Size: 854 92 4176 754 188 590 1455 1842
Fragments arranged by size:
4176 3162 2404 1842 1455 854 754 654
627 590 338 325 217 191 188 92
BanII G_rGCy'C
Cuts at: 1391 2275 3265 5125 6922 7087 7317 8295 10526
Size: 884 990 1860 1797 165 230 978 2231
Cuts at: 10526 10955 12873 12986 14712 15188 1391
Size: 429 1918 113 1726 476 4072
Fragments arranged by size:
4072 2231 1918 1860 1797 1726 990 978
884 476 429 230 165 113
BbeI G_GCGC'C
Cuts at: 8011 14604 8011
Size: 6593 11276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3709 4033 6053 7201 8451 9059 9357
Size: 971 1810 324 2020 1148 1250 608 298
Cuts at: 9357 10518 11372 14082 14375 928
Size: 1161 854 2710 293 4422
Fragments arranged by size:
4422 2710 2020 1810 1250 1161 1148 971
854 608 324 298 293
BbvCI CC'TCA_GC
Cuts at: 13015 14177 13015
Size: 1162 16707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3023 3057 3435 3469 9446 9480 13022
Size: 34 1086 34 378 34 5977 34 3542
Cuts at: 13022 13056 16060 16094 1903
Size: 34 3004 34 3678
Fragments arranged by size:
5977 3678 3542 3004 1086 378 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5017 6696 10339 12448 15761 16971 5017
Size: 1679 3643 2109 3313 1210 5915
Fragments arranged by size:
5915 3643 3313 2109 1679 1210
BclI T'GATC_A
Cuts at: 1548 3793 7464 12355 13797 1548
Size: 2245 3671 4891 1442 5620
Fragments arranged by size:
5620 4891 3671 2245 1442
BfrBI ATG'CAT
Cuts at: 9674 9674
Size: 17869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8620 10239 14716
Size: 107 122 71 631 605 6948 1619 4477
Cuts at: 14716 16480 136
Size: 1764 1525
Fragments arranged by size:
6948 4477 1764 1619 1525 631 605 122
107 71
BglII A'GATC_T
Cuts at: 5834 6673 6904 8557 9152 10617 11835 17864 5834
Size: 839 231 1653 595 1465 1218 6029 5839
Fragments arranged by size:
6029 5839 1653 1465 1218 839 595 231
BlpI GC'TnA_GC
Cuts at: 13926 15286 13926
Size: 1360 16509
Bme1580I G_kGCm'C
Cuts at: 1790 3422 5125 5279 6922 8376 10700 11131 13584
Size: 1632 1703 154 1643 1454 2324 431 2453
Cuts at: 13584 13771 14712 15930 17086 1790
Size: 187 941 1218 1156 2573
Fragments arranged by size:
2573 2453 2324 1703 1643 1632 1454 1218
1156 941 431 187 154
BmgBI CAC'GTC
Cuts at: 1318 4531 13140 1318
Size: 3213 8609 6047
Fragments arranged by size:
8609 6047 3213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 4282 5119
Size: 416 53 83 399 24 1237 1628 837
Cuts at: 5119 6310 8370 9249 10949 13752 15600 16558 442
Size: 1191 2060 879 1700 2803 1848 958 1753
Fragments arranged by size:
2803 2060 1848 1753 1700 1628 1237 1191
958 879 837 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 17869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4471 4643 7560 7785 8618 9850 10760 11419 13003
Size: 172 2917 225 833 1232 910 659 1584
Cuts at: 13003 15181 4471
Size: 2178 7159
Fragments arranged by size:
7159 2917 2178 1584 1232 910 833 659
225 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2375 2741 2837 3228 3287 4231 4410
Size: 997 139 366 96 391 59 944 179
Cuts at: 4410 4460 6515 6778 8468 8912 9416 9725 9845
Size: 50 2055 263 1690 444 504 309 120
Cuts at: 9845 10284 11202 11714 12426 13355 15176 16529 1239
Size: 439 918 512 712 929 1821 1353 2579
Fragments arranged by size:
2579 2055 1821 1690 1353 997 944 929
918 712 512 504 444 439 391 366
309 263 179 139 120 96 59 50
Bpu10I CC'TnA_GC
Cuts at: 7089 13015 13694 14177 14362 7089
Size: 5926 679 483 185 10596
Fragments arranged by size:
10596 5926 679 483 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2858 4019 4922 5101 7619 11198 13829 14655 15994
Size: 1161 903 179 2518 3579 2631 826 1339
Cuts at: 15994 16859 17157 2858
Size: 865 298 3570
Fragments arranged by size:
3579 3570 2631 2518 1339 1161 903 865
826 298 179
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2784 2813 3672 4277 7179 7728 13067
Size: 617 1285 29 859 605 2902 549 5339
Cuts at: 13067 13306 13712 14117 16532 882
Size: 239 406 405 2415 2219
Fragments arranged by size:
5339 2902 2415 2219 1285 859 617 605
549 406 405 239 29
BsaAI yAC'GTr
Cuts at: 493 1204 3468 12510 14881 15660 493
Size: 711 2264 9042 2371 779 2702
Fragments arranged by size:
9042 2702 2371 2264 779 711
BsaBI GATnn'nnATC
Cuts at: 2246 6221 12014 17863 2246
Size: 3975 5793 5849 2252
Fragments arranged by size:
5849 5793 3975 2252
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8008 11772 14601 15676
Size: 53 83 186 936 6475 3764 2829 1075
Cuts at: 15676 16058 16693 275
Size: 382 635 1451
Fragments arranged by size:
6475 3764 2829 1451 1075 936 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3654 7425 7881 7911 10905 11148 11802 12621 13392
Size: 3771 456 30 2994 243 654 819 771
Cuts at: 13392 16302 16700 16974 17121 17532 3654
Size: 2910 398 274 147 411 3991
Fragments arranged by size:
3991 3771 2994 2910 819 771 654 456
411 398 274 243 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2714 2744 3107
Size: 30 165 30 897 30 384 30 363
Cuts at: 3107 3137 3218 3248 4243 4273 4327 4357 5900
Size: 30 81 30 995 30 54 30 1543
Cuts at: 5900 5930 7276 7306 8055 8085 9698 9728 10542
Size: 30 1346 30 749 30 1613 30 814
Cuts at: 10542 10572 12603 12633 13218 13248 14865 14895 1178
Size: 30 2031 30 585 30 1617 30 4152
Fragments arranged by size:
4152 2031 1617 1613 1543 1346 995 897
814 749 585 384 363 165 81 54
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 3440 3546 4124 4694 4781 4856 6694
Size: 1186 1068 106 578 570 87 75 1838
Cuts at: 6694 6714 7095 8303 8692 10356 11948 12516 12537
Size: 20 381 1208 389 1664 1592 568 21
Cuts at: 12537 12976 13832 13844 14165 14833 1186
Size: 439 856 12 321 668 4222
Fragments arranged by size:
4222 1838 1664 1592 1208 1186 1068 856
668 578 570 568 439 389 381 321
106 87 75 21 20 12
BseYI C'CCAG_C
Cuts at: 3802 8052 8924 9044 9946 10293 10938 11680 13726
Size: 4250 872 120 902 347 645 742 2046
Cuts at: 13726 17072 17568 3802
Size: 3346 496 4103
Fragments arranged by size:
4250 4103 3346 2046 902 872 742 645
496 347 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4665 7544 7946 10725 11989 14686 14987
Size: 573 2929 2879 402 2779 1264 2697 301
Cuts at: 14987 1163
Size: 4045
Fragments arranged by size:
4045 2929 2879 2779 2697 1264 573 402
301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5185 15319 16080 17108 1540
Size: 275 186 3184 10134 761 1028 2301
Fragments arranged by size:
10134 3184 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4436 4629 4938 8095 9495 10285 10614 10700 11131
Size: 193 309 3157 1400 790 329 86 431
Cuts at: 11131 12873 13369 13584 13771 14838 14993 15930 16015
Size: 1742 496 215 187 1067 155 937 85
Cuts at: 16015 17086 4436
Size: 1071 5219
Fragments arranged by size:
5219 3157 1742 1400 1071 1067 937 790
496 431 329 309 215 193 187 155
86 85
BsmI GAATG_Cn'
Cuts at: 2980 4224 7009 7554 9674 10036 11610 11763 12972
Size: 1244 2785 545 2120 362 1574 153 1209
Cuts at: 12972 13977 15380 15473 2980
Size: 1005 1403 93 5376
Fragments arranged by size:
5376 2785 2120 1574 1403 1244 1209 1005
545 362 153 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5348 6111 10361 5348
Size: 763 4250 12856
Fragments arranged by size:
12856 4250 763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 3265 3422 4436 4629 4938 5125
Size: 399 485 990 157 1014 193 309 187
Cuts at: 5125 5279 6922 7087 7317 8095 8295 8376 9495
Size: 154 1643 165 230 778 200 81 1119
Cuts at: 9495 10285 10526 10614 10700 10955 11131 12873 12986
Size: 790 241 88 86 255 176 1742 113
Cuts at: 12986 13369 13584 13771 14712 14838 14993 15188 15930
Size: 383 215 187 941 126 155 195 742
Cuts at: 15930 16015 17086 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1742 1643 1119 1071 1014 990 941
790 778 742 485 399 383 309 255
241 230 215 200 195 193 187 187
176 165 157 155 154 126 113 88
86 85 81
BspEI T'CCGG_A
Cuts at: 7881 7911 10905 11802 12621 17532 7881
Size: 30 2994 897 819 4911 8218
Fragments arranged by size:
8218 4911 2994 897 819 30
BspHI T'CATG_A
Cuts at: 3181 6570 15758 17488 3181
Size: 3389 9188 1730 3562
Fragments arranged by size:
9188 3562 3389 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2890 6262 6314 6625 8623 12194 14959 15224
Size: 2046 3372 52 311 1998 3571 2765 265
Cuts at: 15224 844
Size: 3489
Fragments arranged by size:
3571 3489 3372 2765 2046 1998 311 265
52
BsrBI CCG'CTC
Cuts at: 1394 1823 13026 14398 15756 1394
Size: 429 11203 1372 1358 3507
Fragments arranged by size:
11203 3507 1372 1358 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4921 7539 9768 12786 13705 16364 16538
Size: 1183 3673 2618 2229 3018 919 2659 174
Cuts at: 16538 65
Size: 1396
Fragments arranged by size:
3673 3018 2659 2618 2229 1396 1183 919
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2853 4920 10653 13721 14447 16513 16700
Size: 110 1043 2067 5733 3068 726 2066 187
Cuts at: 16700 1700
Size: 2869
Fragments arranged by size:
5733 3068 2869 2067 2066 1043 726 187
110
BsrGI T'GTAC_A
Cuts at: 96 2569 6663 8847 13633 96
Size: 2473 4094 2184 4786 4332
Fragments arranged by size:
4786 4332 4094 2473 2184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 17869
BssSI C'ACGA_G
Cuts at: 3042 3454 6659 14992 15929 16941 3042
Size: 412 3205 8333 937 1012 3970
Fragments arranged by size:
8333 3970 3205 1012 937 412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 7015 7933 794
Size: 6221 918 10730
Fragments arranged by size:
10730 6221 918
BstBI TT'CG_AA
Cuts at: 16673 16673
Size: 17869
BstEII G'GTnAC_C
Cuts at: 1184 14971 1184
Size: 13787 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 2692 9004 11317 14593 2692
Size: 6312 2313 3276 5968
Fragments arranged by size:
6312 5968 3276 2313
BstYI r'GATC_y
Cuts at: 1972 3411 3811 4207 4603 5834 6501 6673 6904
Size: 1439 400 396 396 1231 667 172 231
Cuts at: 6904 7609 7630 8479 8557 8722 8812 8902 9152
Size: 705 21 849 78 165 90 90 250
Cuts at: 9152 9604 9907 10534 10617 11825 11835 12516 14026
Size: 452 303 627 83 1208 10 681 1510
Cuts at: 14026 14095 15199 15566 15951 15968 17420 17506 17518
Size: 69 1104 367 385 17 1452 86 12
Cuts at: 17518 17864 1972
Size: 346 1977
Fragments arranged by size:
1977 1510 1452 1439 1231 1208 1104 849
705 681 667 627 452 400 396 396
385 367 346 303 250 231 172 165
90 90 86 83 78 69 21 17
12 10
BstZ17I GTA'TAC
Cuts at: 3471 3471
Size: 17869
Bsu36I CC'TnA_GG
Cuts at: 3973 4152 7548 3973
Size: 179 3396 14294
Fragments arranged by size:
14294 3396 179
BtgI C'CryG_G
Cuts at: 513 1065 2679 5922 7590 7789 8689 8746 13257
Size: 552 1614 3243 1668 199 900 57 4511
Cuts at: 13257 14455 14950 15036 513
Size: 1198 495 86 3346
Fragments arranged by size:
4511 3346 3243 1668 1614 1198 900 552
495 199 86 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 14788 487
Size: 683 56 13562 3568
Fragments arranged by size:
13562 3568 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 3401 3441 5511 6391 7469 7515 8710 9810
Size: 112 40 2070 880 1078 46 1195 1100
Cuts at: 9810 11115 11131 13077 13752 14929 15004 15385 16179
Size: 1305 16 1946 675 1177 75 381 794
Cuts at: 16179 16199 3289
Size: 20 4959
Fragments arranged by size:
4959 2070 1946 1305 1195 1177 1100 1078
880 794 675 381 112 75 46 40
20 16
ClaI AT'CG_AT
Cuts at: 1554 3187 5282 10152 1554
Size: 1633 2095 4870 9271
Fragments arranged by size:
9271 4870 2095 1633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2418 2453 4527 4562 529
Size: 35 1854 35 2074 35 13836
Fragments arranged by size:
13836 2074 1854 35 35 35
DraI TTT'AAA
Cuts at: 4882 7855 12912 15173 4882
Size: 2973 5057 2261 7578
Fragments arranged by size:
7578 5057 2973 2261
DraIII CAC_nnn'GTG
Cuts at: 4966 7567 10695 10704 4966
Size: 2601 3128 9 12131
Fragments arranged by size:
12131 3128 2601 9
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 16876 809
Size: 507 15560 1802
Fragments arranged by size:
15560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 4320 4918
Size: 54 1425 50 275 18 852 1638 598
Cuts at: 4918 8422 10316 15316 16205 17834 8
Size: 3504 1894 5000 889 1629 43
Fragments arranged by size:
5000 3504 1894 1638 1629 1425 889 852
598 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 15316 1537
Size: 275 13504 4090
Fragments arranged by size:
13504 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 3129 3402 3627 3714 4275 4964 5307
Size: 1391 385 273 225 87 561 689 343
Cuts at: 5307 7408 8354 9335 10531 10839 11083 14771 15799
Size: 2101 946 981 1196 308 244 3688 1028
Cuts at: 15799 1353
Size: 3423
Fragments arranged by size:
3688 3423 2101 1391 1196 1028 981 946
689 561 385 343 308 273 244 225
87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4889 14762 16456 16830 16976 387
Size: 1064 138 3300 9873 1694 374 146 1280
Fragments arranged by size:
9873 3300 1694 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 12871 12871
Size: 17869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2236 2375 2741 2837 3228 3287
Size: 393 554 50 139 366 96 391 59
Cuts at: 3287 3426 4035 4064 4231 4299 4410 4460 4584
Size: 139 609 29 167 68 111 50 124
Cuts at: 4584 5007 5288 5437 5826 5882 6366 6515 6778
Size: 423 281 149 389 56 484 149 263
Cuts at: 6778 6869 7589 8217 8378 8468 8912 9416 9725
Size: 91 720 628 161 90 444 504 309
Cuts at: 9725 9845 10064 10199 10257 10284 10394 11202 11714
Size: 120 219 135 58 27 110 808 512
Cuts at: 11714 11875 12426 12648 13251 13355 14271 15176 15932
Size: 161 551 222 603 104 916 905 756
Cuts at: 15932 16529 17316 17584 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 916 905 808 787 756 720 628
609 603 597 554 551 512 504 484
444 423 393 391 389 366 309 281
268 263 222 219 167 161 161 149
149 139 139 135 124 120 111 110
104 96 91 90 68 59 58 56
50 50 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 14180 14180
Size: 17869
EcoO109I rG'GnC_Cy
Cuts at: 3897 5121 6918 8928 10977 12577 12714 14035 14290
Size: 1224 1797 2010 2049 1600 137 1321 255
Cuts at: 14290 14708 14709 15058 3897
Size: 418 1 349 6708
Fragments arranged by size:
6708 2049 2010 1797 1600 1321 1224 418
349 255 137 1
EcoRI G'AATT_C
Cuts at: 2867 5722 6962 7397 8820 9637 14795 15178 15556
Size: 2855 1240 435 1423 817 5158 383 378
Cuts at: 15556 2867
Size: 5180
Fragments arranged by size:
5180 5158 2855 1423 1240 817 435 383
378
EcoRV GAT'ATC
Cuts at: 2937 9730 10408 14149 2937
Size: 6793 678 3741 6657
Fragments arranged by size:
6793 6657 3741 678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2216 2360 4430 4711 5255 6148 7136 13223 13256
Size: 144 2070 281 544 893 988 6087 33
Cuts at: 13256 2216
Size: 6829
Fragments arranged by size:
6829 6087 2070 988 893 544 281 144
33
FspI TGC'GCA
Cuts at: 16375 16375
Size: 17869
HaeII r_GCGC'y
Cuts at: 1678 8011 8059 10100 10654 14604 17016 1678
Size: 6333 48 2041 554 3950 2412 2531
Fragments arranged by size:
6333 3950 2531 2412 2041 554 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 3038 3070 3359
Size: 32 135 32 494 32 940 32 289
Cuts at: 3359 3391 3471 3503 4661 4693 5219 5251 5264
Size: 32 80 32 1158 32 526 32 13
Cuts at: 5264 5296 6008 6040 6236 6268 6503 6520 6535
Size: 32 712 32 196 32 235 17 15
Cuts at: 6535 6552 7060 7092 7276 7308 8207 8239 8547
Size: 17 508 32 184 32 899 32 308
Cuts at: 8547 8579 9864 9896 10481 10513 10542 10574 11195
Size: 32 1285 32 585 32 29 32 621
Cuts at: 11195 11227 11797 11829 12212 12244 13789 13821 14365
Size: 32 570 32 383 32 1545 32 544
Cuts at: 14365 14397 14600 14632 14994 15026 16510 16542 16584
Size: 32 203 32 362 32 1484 32 42
Cuts at: 16584 16616 1373
Size: 32 2626
Fragments arranged by size:
2626 1545 1484 1285 1158 940 899 712
621 585 570 544 526 508 494 383
362 308 289 235 203 196 184 135
80 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 29 17 17
15 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5913 7423 11776 12421 13741 15213
Size: 988 281 3975 1510 4353 645 1320 1472
Cuts at: 15213 15459 669
Size: 246 3079
Fragments arranged by size:
4353 3975 3079 1510 1472 1320 988 645
281 246
HindIII A'AGCT_T
Cuts at: 748 5449 5808 6453 12239 748
Size: 4701 359 645 5786 6378
Fragments arranged by size:
6378 5786 4701 645 359
HpaI GTT'AAC
Cuts at: 7423 15459 7423
Size: 8036 9833
KasI G'GCGC_C
Cuts at: 8007 14600 8007
Size: 6593 11276
KpnI G_GTAC'C
Cuts at: 4953 13662 14416 15194 4953
Size: 8709 754 778 7628
Fragments arranged by size:
8709 7628 778 754
MfeI C'AATT_G
Cuts at: 4504 9597 9998 12391 15468 4504
Size: 5093 401 2393 3077 6905
Fragments arranged by size:
6905 5093 3077 2393 401
MluI A'CGCG_T
Cuts at: 17745 17745
Size: 17869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2298 3107 4468 4806 5120 5229 7309
Size: 684 309 809 1361 338 314 109 2080
Cuts at: 7309 7325 7554 9006 9850 12870 14034 16983 17167
Size: 16 229 1452 844 3020 1164 2949 184
Cuts at: 17167 1305
Size: 2007
Fragments arranged by size:
3020 2949 2080 2007 1452 1361 1164 844
809 684 338 314 309 229 184 109
16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2684 4322 8424 10318 10
Size: 54 1425 343 852 1638 4102 1894 7561
Fragments arranged by size:
7561 4102 1894 1638 1425 852 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4439 4662 4931 6627 7901 8855 8873
Size: 648 3273 223 269 1696 1274 954 18
Cuts at: 8873 9488 9551 10421 10896 13256 14118 14807 15827
Size: 615 63 870 475 2360 862 689 1020
Cuts at: 15827 16186 16345 518
Size: 359 159 2042
Fragments arranged by size:
3273 2360 2042 1696 1274 1020 954 870
862 689 648 615 475 359 269 223
159 63 18
MspA1I CmG'CkG
Cuts at: 1606 2815 2845 3806 4774 7990 8748 9084 9757
Size: 1209 30 961 968 3216 758 336 673
Cuts at: 9757 9928 11657 11788 12943 13594 13730 13925 14541
Size: 171 1729 131 1155 651 136 195 616
Cuts at: 14541 14658 15154 15285 15962 17110 17355 1606
Size: 117 496 131 677 1148 245 2120
Fragments arranged by size:
3216 2120 1729 1209 1155 1148 968 961
758 677 673 651 616 496 336 245
195 171 136 131 131 117 30
NaeI GCC'GGC
Cuts at: 10655 14449 10655
Size: 3794 14075
NarI GG'CG_CC
Cuts at: 8008 14601 8008
Size: 6593 11276
NcoI C'CATG_G
Cuts at: 513 1065 2679 5922 7590 7789 8689 13257 14455
Size: 552 1614 3243 1668 199 900 4568 1198
Cuts at: 14455 14950 15036 513
Size: 495 86 3346
Fragments arranged by size:
4568 3346 3243 1668 1614 1198 900 552
495 199 86
NdeI CA'TA_TG
Cuts at: 387 5038 5482 13411 387
Size: 4651 444 7929 4845
Fragments arranged by size:
7929 4845 4651 444
NgoMIV G'CCGG_C
Cuts at: 10653 14447 10653
Size: 3794 14075
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 17869
NotI GC'GGCC_GC
Cuts at: 15316 15316
Size: 17869
NsiI A_TGCA'T
Cuts at: 9676 9676
Size: 17869
NspI r_CATG'y
Cuts at: 1132 1229 4517 4547 6626 7586 7788 12046 13612
Size: 97 3288 30 2079 960 202 4258 1566
Cuts at: 13612 15227 16772 1132
Size: 1615 1545 2229
Fragments arranged by size:
4258 3288 2229 2079 1615 1566 1545 960
202 97 30
PacI TTA_AT'TAA
Cuts at: 13530 13530
Size: 17869
PasI CC'CwG_GG
Cuts at: 1348 8295 8311 10082 12125 14713 1348
Size: 6947 16 1771 2043 2588 4504
Fragments arranged by size:
6947 4504 2588 2043 1771 16
PciI A'CATG_T
Cuts at: 4543 16768 4543
Size: 12225 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4483 8491 10547 11349 11407 11615 14461 1108
Size: 3375 4008 2056 802 58 208 2846 4516
Fragments arranged by size:
4516 4008 3375 2846 2056 802 208 58
PfoI T'CCnGG_A
Cuts at: 2718 3244 3256 3316 4247 4518 4615 7241 8073
Size: 526 12 60 931 271 97 2626 832
Cuts at: 8073 8484 9702 12747 14163 2718
Size: 411 1218 3045 1416 6424
Fragments arranged by size:
6424 3045 2626 1416 1218 931 832 526
411 271 97 60 12
PmeI GTTT'AAAC
Cuts at: 15173 15173
Size: 17869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12603 12635 13303 13335 15938 15970 17451 17483 12603
Size: 32 668 32 2603 32 1481 32 12989
Fragments arranged by size:
12989 2603 1481 668 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 8928 10977 12577 12714 15058 8928
Size: 2049 1600 137 2344 11739
Fragments arranged by size:
11739 2344 2049 1600 137
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 17869
PsiI TTA'TAA
Cuts at: 5883 10275 14498 15439 5883
Size: 4392 4223 941 8313
Fragments arranged by size:
8313 4392 4223 941
PspOMI G'GGCC_C
Cuts at: 5121 6918 14708 5121
Size: 1797 7790 8282
Fragments arranged by size:
8282 7790 1797
PspXI vC'TCGA_Gb
Cuts at: 3587 3587
Size: 17869
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2013 2045 3896 3928 10570 10602 13608 13640 2013
Size: 32 1851 32 6642 32 3006 32 6242
Fragments arranged by size:
6642 6242 3006 1851 32 32 32 32
PstI C_TGCA'G
Cuts at: 830 3297 3448 3784 3811 6273 7522 7995 8509
Size: 2467 151 336 27 2462 1249 473 514
Cuts at: 8509 9805 9942 11374 11923 13072 13452 15221 830
Size: 1296 137 1432 549 1149 380 1769 3478
Fragments arranged by size:
3478 2467 2462 1769 1432 1296 1249 1149
549 514 473 380 336 151 137 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 17869
PvuII CAG'CTG
Cuts at: 2815 2845 3806 7990 9084 9757 9928 11657 11788
Size: 30 961 4184 1094 673 171 1729 131
Cuts at: 11788 12943 13594 13730 13925 15154 2815
Size: 1155 651 136 195 1229 5530
Fragments arranged by size:
5530 4184 1729 1229 1155 1094 961 673
651 195 171 136 131 30
SacI G_AGCT'C
Cuts at: 12873 12873
Size: 17869
SacII CC_GC'GG
Cuts at: 8749 8749
Size: 17869
SalI G'TCGA_C
Cuts at: 1936 15211 1936
Size: 13275 4594
SanDI GG'GwC_CC
Cuts at: 8928 8928
Size: 17869
SapI GCTCTTCn'nnn_
Cuts at: 10531 14771 10531
Size: 4240 13629
SbfI CC_TGCA'GG
Cuts at: 15221 15221
Size: 17869
ScaI AGT'ACT
Cuts at: 5299 5988 16117 17829 5299
Size: 689 10129 1712 5339
Fragments arranged by size:
10129 5339 1712 689
SexAI A'CCwGG_T
Cuts at: 13315 13315
Size: 17869
SfcI C'TryA_G
Cuts at: 826 1045 3293 3444 3687 3780 3807 4571 5318
Size: 219 2248 151 243 93 27 764 747
Cuts at: 5318 5727 6269 6507 7518 7801 7991 8505 9321
Size: 409 542 238 1011 283 190 514 816
Cuts at: 9321 9801 9938 11297 11370 11919 13068 13448 15217
Size: 480 137 1359 73 549 1149 380 1769
Cuts at: 15217 16352 17033 17224 826
Size: 1135 681 191 1471
Fragments arranged by size:
2248 1769 1471 1359 1149 1135 1011 816
764 747 681 549 542 514 480 409
380 283 243 238 219 191 190 151
137 93 73 27
SfoI GGC'GCC
Cuts at: 8009 14602 8009
Size: 6593 11276
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 17869
SmaI CCC'GGG
Cuts at: 1872 15196 1872
Size: 13324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 3587 3998 4937 5080 7077
Size: 197 926 930 714 411 939 143 1997
Cuts at: 7077 7634 11177 13844 14030 14670 15973 16874 17136
Size: 557 3543 2667 186 640 1303 901 262
Cuts at: 17136 820
Size: 1553
Fragments arranged by size:
3543 2667 1997 1553 1303 939 930 926
901 714 640 557 411 262 197 186
143
SnaBI TAC'GTA
Cuts at: 493 12510 15660 493
Size: 12017 3150 2702
Fragments arranged by size:
12017 3150 2702
SpeI A'CTAG_T
Cuts at: 152 5195 152
Size: 5043 12826
SphI G_CATG'C
Cuts at: 1132 12046 15227 1132
Size: 10914 3181 3774
Fragments arranged by size:
10914 3774 3181
SspI AAT'ATT
Cuts at: 5 52 2446 3071 11053 15793 5
Size: 47 2394 625 7982 4740 2081
Fragments arranged by size:
7982 4740 2394 2081 625 47
StuI AGG'CCT
Cuts at: 1517 2016 3093 4975 12835 1517
Size: 499 1077 1882 7860 6551
Fragments arranged by size:
7860 6551 1882 1077 499
StyI C'CwwG_G
Cuts at: 513 1065 2234 2679 2925 4056 5397 5922 6041
Size: 552 1169 445 246 1131 1341 525 119
Cuts at: 6041 6922 7590 7789 8347 8689 9121 9159 10056
Size: 881 668 199 558 342 432 38 897
Cuts at: 10056 10313 10386 10449 12459 13257 14049 14127 14455
Size: 257 73 63 2010 798 792 78 328
Cuts at: 14455 14557 14950 15036 15308 513
Size: 102 393 86 272 3074
Fragments arranged by size:
3074 2010 1341 1169 1131 897 881 798
792 668 558 552 525 445 432 393
342 328 272 257 246 199 119 102
86 78 73 63 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3650 4085 4250 5158 5199 6329
Size: 1255 647 1423 435 165 908 41 1130
Cuts at: 6329 7161 9134 10744 10910 11309 12904 15896 15913
Size: 832 1973 1610 166 399 1595 2992 17
Cuts at: 15913 16066 16251 16696 325
Size: 153 185 445 1498
Fragments arranged by size:
2992 1973 1610 1595 1498 1423 1255 1130
908 832 647 445 435 399 185 166
165 153 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 5297 5986
Size: 274 80 33 51 1443 592 2728 689
Cuts at: 5986 6663 8847 10586 13633 14403 16115 17827 96
Size: 677 2184 1739 3047 770 1712 1712 138
Fragments arranged by size:
3047 2728 2184 1739 1712 1712 1443 770
689 677 592 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 4327 4359 5133 5165 7827 7859 8055
Size: 32 1188 32 774 32 2662 32 196
Cuts at: 8055 8087 9835 9867 9892 9924 13563 13595 14865
Size: 32 1748 32 25 32 3639 32 1270
Cuts at: 14865 14897 17829 17861 3107
Size: 32 2932 32 3115
Fragments arranged by size:
3639 3115 2932 2662 1748 1270 1188 774
196 32 32 32 32 32 32 32
32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 1316 8450 10970 1316
Size: 7134 2520 8215
Fragments arranged by size:
8215 7134 2520
XbaI T'CTAG_A
Cuts at: 14092 15205 14092
Size: 1113 16756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4484 5162 5929 10399 12646 13264 4484
Size: 678 767 4470 2247 618 9089
Fragments arranged by size:
9089 4470 2247 767 678 618
XhoI C'TCGA_G
Cuts at: 1943 3587 7077 1943
Size: 1644 3490 12735
Fragments arranged by size:
12735 3490 1644
XmaI C'CCGG_G
Cuts at: 1870 15194 1870
Size: 13324 4545
XmnI GAAnn'nnTTC
Cuts at: 3177 3634 4413 5943 8305 9077 10848 12743 12934
Size: 457 779 1530 2362 772 1771 1895 191
Cuts at: 12934 13236 15998 3177
Size: 302 2762 5048
Fragments arranged by size:
5048 2762 2362 1895 1771 1530 779 772
457 302 191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11773 15677 276
Size: 53 83 186 936 10239 3904 2468
Fragments arranged by size:
10239 3904 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BaeI BsiWI FseI FspAI NruI PmlI RsrII
SfiI SrfI SwaI