Restriction map for
pFN21AE2490
(Circular) (Six-base) MAPSORT of: pFN21AE2490.seq Check: 3691 from: 1 to: 5989
pFN21AE2490 5989 bp
With 182 enzymes: SgfI *
November 15, 2013 16:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 2479 3310 2479
Size: 831 5158
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2443 4052 5436 5704 1632
Size: 811 1609 1384 268 1917
Fragments arranged by size:
1917 1609 1384 811 268
AfeI AGC'GCT
Cuts at: 2062 2062
Size: 5989
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 2204 1166
Size: 1038 4951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 3274 3732 5304 3274
Size: 458 1572 3959
Fragments arranged by size:
3959 1572 458
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2187 2545 3298 3524 3676 5785 1493
Size: 694 358 753 226 152 2109 1697
Fragments arranged by size:
2109 1697 753 694 358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 3314 1870
Size: 73 1371 4545
Fragments arranged by size:
4545 1371 73
AvrII C'CTAG_G
Cuts at: 2602 2666 2602
Size: 64 5925
BamHI G'GATC_C
Cuts at: 2510 3319 3686 2510
Size: 809 367 4813
Fragments arranged by size:
4813 809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2479 3310 4765 618
Size: 325 217 627 692 831 1455 1842
Fragments arranged by size:
1842 1455 831 692 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2325 2776 2910 3308 1391
Size: 934 451 134 398 4072
Fragments arranged by size:
4072 934 451 398 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 928
Size: 971 204 4814
Fragments arranged by size:
4814 971 204
BbvCI CC'TCA_GC
Cuts at: 2927 2927
Size: 5989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2056 3881 5091 2056
Size: 1825 1210 2954
Fragments arranged by size:
2954 1825 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3248 4600 136
Size: 107 122 71 631 605 1576 1352 1525
Fragments arranged by size:
1576 1525 1352 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2454 5984 2454
Size: 3530 2459
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2123 2802 4050 5206 1790
Size: 234 99 679 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 679 234 99
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 2632 3720
Size: 416 53 83 399 24 952 263 1088
Cuts at: 3720 4678 442
Size: 958 1753
Fragments arranged by size:
1753 1088 958 952 416 399 263 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 4649 1239
Size: 1559 1851 2579
Fragments arranged by size:
2579 1851 1559
Bpu10I CC'TnA_GC
Cuts at: 2295 2927 2295
Size: 632 5357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4114 4979 5277 4114
Size: 865 298 4826
Fragments arranged by size:
4826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 4652 882
Size: 617 804 2349 2219
Fragments arranged by size:
2349 2219 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2808 4422 4820 5094 5241 5652 2808
Size: 1614 398 274 147 411 3145
Fragments arranged by size:
3145 1614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2803 2833 1178
Size: 30 165 30 1400 30 4334
Fragments arranged by size:
4334 1400 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2975 1186
Size: 1789 4200
BseYI C'CCAG_C
Cuts at: 3237 5192 5688 3237
Size: 1955 496 3538
Fragments arranged by size:
3538 1955 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 4924
Fragments arranged by size:
4924 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 4050 4135 5206 2177
Size: 1873 85 1071 2960
Fragments arranged by size:
2960 1873 1071 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
BsmBI CGTCTCn'nnnn_
Cuts at: 3224 3224
Size: 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2123 2177 2325 2776 2802 2910
Size: 399 234 99 54 148 451 26 108
Cuts at: 2910 3308 4050 4135 5206 1391
Size: 398 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 398 234 148
108 99 85 54 26
BspEI T'CCGG_A
Cuts at: 2808 5652 2808
Size: 2844 3145
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3344 844
Size: 2500 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 794
Size: 1421 4568
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstXI CCAn_nnnn'nTGG
Cuts at: 2876 2876
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2454 2510 2847 3139 3319 3686 4071 4088
Size: 482 56 337 292 180 367 385 17
Cuts at: 4088 5540 5626 5638 5984 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 482 385 367 346 337 292
180 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3115 3115
Size: 5989
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 2497 3505 4299 4319 2497
Size: 1008 794 20 4167
Fragments arranged by size:
4167 1008 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3054 3293 3054
Size: 239 5750
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3194 4996 809
Size: 507 1878 1802 1802
Fragments arranged by size:
1878 1802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2334 2569
Size: 54 1425 50 275 18 177 327 235
Cuts at: 2569 3436 4325 5954 8
Size: 867 889 1629 43
Fragments arranged by size:
1629 1425 889 867 327 275 235 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3919 1353
Size: 2566 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2443 2798 4052 4649 5436 5704 1239
Size: 393 811 355 1254 597 787 268 1524
Fragments arranged by size:
1524 1254 811 787 597 393 355 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3164 3164
Size: 5989
EcoO109I rG'GnC_Cy
Cuts at: 2592 2592
Size: 5989
EcoRI G'AATT_C
Cuts at: 2187 3298 3676 2187
Size: 1111 378 4500
Fragments arranged by size:
4500 1111 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 3190 2473
Size: 717 5272
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2064 2139 5136 1678
Size: 386 75 2997 2531
Fragments arranged by size:
2997 2531 386 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373
Size: 32 135 32 3058 32 42 32 2626
Fragments arranged by size:
3058 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3016 3333 3579 669
Size: 988 281 1078 317 246 3079
Fragments arranged by size:
3079 1078 988 317 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 2483 3314 2483
Size: 831 5158
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 2814 5103 5287 1305
Size: 1233 276 2289 184 2007
Fragments arranged by size:
2289 2007 1233 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2336 2571 10
Size: 54 1425 343 177 327 235 3428
Fragments arranged by size:
3428 1425 343 327 235 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2204 3023 3947 4306 4465 518
Size: 648 873 165 819 924 359 159 2042
Fragments arranged by size:
2042 924 873 819 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2563 3405 4082 5230 5475 1606
Size: 957 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 957 842 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 3347 4892 1132
Size: 97 2118 1545 2229
Fragments arranged by size:
2229 2118 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2767 1348
Size: 1419 4570
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2016 1108
Size: 908 5081
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2592 2592
Size: 5989
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 2709 3559 2709
Size: 850 5139
PstI C_TGCA'G
Cuts at: 830 3341 830
Size: 2511 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2563 2563
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 3337 4472 5153 5344 826
Size: 219 2292 1135 681 191 1471
Fragments arranged by size:
2292 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3248 3248
Size: 5989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4093 4994 5256 820
Size: 197 926 2150 901 262 1553
Fragments arranged by size:
2150 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2427 2595 2602 2666 3243 3428
Size: 552 939 423 168 7 64 577 185
Cuts at: 3428 513
Size: 3074
Fragments arranged by size:
3074 939 577 552 423 185 168 64
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 3021 4016 4033 4186 4371 4816
Size: 1255 1053 388 995 17 153 185 445
Cuts at: 4816 325
Size: 1498
Fragments arranged by size:
1498 1255 1053 995 445 388 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4235 5947 96
Size: 274 80 33 51 1443 2258 1712 138
Fragments arranged by size:
2258 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 5949 5981 2828
Size: 32 3089 32 2836
Fragments arranged by size:
3089 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 2236 3325 2236
Size: 1089 4900
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI XcmI