Restriction map for
pFN21AE2489
(Circular) (Six-base) MAPSORT of: pFN21AE2489.seq Check: 921 from: 1 to: 6445
pFN21AE2489 6445 bp
With 182 enzymes: SgfI *
November 15, 2013 16:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2965 3788 1937
Size: 1028 823 4594
Fragments arranged by size:
4594 1028 823
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2537 2739 4508 5892 6160 1632
Size: 797 108 202 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 797 268 202 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 2611 5174 2611
Size: 2563 3882
AleI CACnn'nnGTG
Cuts at: 1166 2985 3119 3716 1166
Size: 1819 134 597 3895
Fragments arranged by size:
3895 1819 597 134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AlwNI CAG_nnn'CTG
Cuts at: 3217 4188 5760 3217
Size: 971 1572 3902
Fragments arranged by size:
3902 1572 971
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 2303 2449 3314 3754 3980 4132 6241 1493
Size: 810 146 865 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 865 810 440 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 2766 4999 160
Size: 872 1734 2233 1606
Fragments arranged by size:
2233 1734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 3450 3770 1870
Size: 73 1507 320 4545
Fragments arranged by size:
4545 1507 320 73
AvrII C'CTAG_G
Cuts at: 2375 2375
Size: 6445
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3766 5221 618
Size: 325 217 627 1979 1455 1842
Fragments arranged by size:
1979 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3636 3764 1391
Size: 2245 128 4072
Fragments arranged by size:
4072 2245 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3173 928
Size: 971 1274 4200
Fragments arranged by size:
4200 1274 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2694 2887 3256 6440 2694
Size: 193 369 3184 2699
Fragments arranged by size:
3184 2699 369 193
BlpI GC'TnA_GC
Cuts at: 3630 3862 3630
Size: 232 6213
Bme1580I G_kGCm'C
Cuts at: 1790 2982 4506 5662 1790
Size: 1192 1524 1156 2573
Fragments arranged by size:
2573 1524 1192 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3583 4176
Size: 416 53 83 399 24 732 1434 593
Cuts at: 4176 5134 442
Size: 958 1753
Fragments arranged by size:
1753 1434 958 732 593 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2880 3620 3699 5105 1239
Size: 1345 296 740 79 1406 2579
Fragments arranged by size:
2579 1406 1345 740 296 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2253 3074 4570 5435 5733 2253
Size: 821 1496 865 298 2965
Fragments arranged by size:
2965 1496 865 821 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5108 882
Size: 617 3609 2219
Fragments arranged by size:
3609 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3601 4236 493
Size: 711 2397 635 2702
Fragments arranged by size:
2702 2397 711 635
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4252 4634 5269 275
Size: 53 83 186 936 2719 382 635 1451
Fragments arranged by size:
2719 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6220
Fragments arranged by size:
6220 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2505 3165 3509 1186
Size: 856 463 660 344 4122
Fragments arranged by size:
4122 856 660 463 344
BseYI C'CCAG_C
Cuts at: 5648 6144 5648
Size: 496 5949
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2221 1163
Size: 573 485 5387
Fragments arranged by size:
5387 573 485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3413 3636 4506 4591 5662 3413
Size: 223 870 85 1071 4196
Fragments arranged by size:
4196 1071 870 223 85
BsmI GAATG_Cn'
Cuts at: 2352 2833 3956 4049 2352
Size: 481 1123 93 4748
Fragments arranged by size:
4748 1123 481 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2153 2153
Size: 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2982 3413 3636 3764 4506 4591 5662
Size: 399 1192 431 223 128 742 85 1071
Cuts at: 5662 1391
Size: 2174
Fragments arranged by size:
2174 1192 1071 742 431 399 223 128
85
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 2188 4334 6064 2188
Size: 2146 1730 2569
Fragments arranged by size:
2569 2146 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3800 844
Size: 2956 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4332 1394
Size: 429 2509 3507
Fragments arranged by size:
3507 2509 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5089 5276 1700
Size: 110 3279 187 2869
Fragments arranged by size:
3279 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 4505 5517 4505
Size: 1012 5433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2424 794
Size: 1630 4815
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 3494 1184
Size: 2310 4135
BstYI r'GATC_y
Cuts at: 1972 2546 2694 2707 2794 2887 3256 3775 4142
Size: 574 148 13 87 93 369 519 367
Cuts at: 4142 4527 4544 5996 6082 6094 6440 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 574 519 385 369 367 346
148 93 87 86 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5706
Fragments arranged by size:
5706 683 56
BtsI GCAGTG_nn'
Cuts at: 3114 3126 3961 4755 4775 3114
Size: 12 835 794 20 4784
Fragments arranged by size:
4784 835 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3204 5452 809
Size: 507 1888 2248 1802
Fragments arranged by size:
2248 1888 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3507 3892 4781
Size: 54 1425 50 275 18 1677 385 889
Cuts at: 4781 6410 8
Size: 1629 43
Fragments arranged by size:
1677 1629 1425 889 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2032 2451 3428 4375 1353
Size: 679 419 977 947 3423
Fragments arranged by size:
3423 977 947 679 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5032 5406 5552 387
Size: 1064 138 3443 374 146 1280
Fragments arranged by size:
3443 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3634 3634
Size: 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2537 2584 2739 2880 3620 3699
Size: 393 797 108 47 155 141 740 79
Cuts at: 3699 4508 5105 5892 6160 1239
Size: 809 597 787 268 1524
Fragments arranged by size:
1524 809 797 787 740 597 393 268
155 141 108 79 47
EcoO109I rG'GnC_Cy
Cuts at: 2396 3214 3444 2396
Size: 818 230 5397
Fragments arranged by size:
5397 818 230
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2829 2404
Size: 425 6020
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 5592 1678
Size: 3914 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 2858 2890 3160
Size: 32 135 32 1025 32 229 32 270
Cuts at: 3160 3192 3206 3225 3238 3257 5086 5118 5160
Size: 32 14 19 13 19 1829 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 1829 1025 270 229 135 42 32
32 32 32 32 32 32 19 19
14 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2993 3789 4035 669
Size: 988 281 1055 796 246 3079
Fragments arranged by size:
3079 1055 988 796 281 246
HindIII A'AGCT_T
Cuts at: 748 2748 2850 748
Size: 2000 102 4343
Fragments arranged by size:
4343 2000 102
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 3062 3539 4044 3062
Size: 477 505 5463
Fragments arranged by size:
5463 505 477
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3050 5559 5743 1305
Size: 1745 2509 184 2007
Fragments arranged by size:
2509 2007 1745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 10
Size: 54 1425 343 1677 2946
Fragments arranged by size:
2946 1677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2985 3119 3716 4403 4762 4921 518
Size: 648 1819 134 597 687 359 159 2042
Fragments arranged by size:
2042 1819 687 648 597 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 3629 3861 4538 5686 5931 1606
Size: 2023 232 677 1148 245 2120
Fragments arranged by size:
2120 2023 1148 677 245 232
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3803 5348 1132
Size: 97 2574 1545 2229
Fragments arranged by size:
2574 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6445
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3505 1108
Size: 2397 4048
PfoI T'CCnGG_A
Cuts at: 3636 3636
Size: 6445
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3214 3214
Size: 6445
PshAI GACnn'nnGTC
Cuts at: 1613 2054 1613
Size: 441 6004
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2832 3121 3797 830
Size: 2002 289 676 3478
Fragments arranged by size:
3478 2002 676 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 3629 3629
Size: 6445
SacI G_AGCT'C
Cuts at: 3636 3636
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SapI GCTCTTCn'nnn_
Cuts at: 3428 3428
Size: 6445
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 3049 4693 6405 3049
Size: 1644 1712 3089
Fragments arranged by size:
3089 1712 1644
SfcI C'TryA_G
Cuts at: 826 1045 2828 3117 3793 4928 5609 5800 826
Size: 219 1783 289 676 1135 681 191 1471
Fragments arranged by size:
1783 1471 1135 681 676 289 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2268 3053 4549 5450 5712 820
Size: 197 926 325 785 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 785 325 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SspI AAT'ATT
Cuts at: 5 52 2176 3330 4369 5
Size: 47 2124 1154 1039 2081
Fragments arranged by size:
2124 2081 1154 1039 47
StuI AGG'CCT
Cuts at: 1517 2125 1517
Size: 608 5837
StyI C'CwwG_G
Cuts at: 513 1065 2004 2375 2937 3588 3884 513
Size: 552 939 371 562 651 296 3074
Fragments arranged by size:
3074 939 651 562 552 371 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4472 4489 4642 4827 5272 325
Size: 1255 2892 17 153 185 445 1498
Fragments arranged by size:
2892 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2105 3047 4691
Size: 274 80 33 51 1443 128 942 1644
Cuts at: 4691 6403 96
Size: 1712 138
Fragments arranged by size:
1712 1644 1443 942 274 138 128 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6405 6437 6405
Size: 32 6413
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 4574 4574
Size: 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI