Restriction map for
pFN21AE2485
(Circular) (Six-base) MAPSORT of: pFN21AE2485.seq Check: 6210 from: 1 to: 6853
pFN21AE2485 6853 bp
With 182 enzymes: SgfI *
October 10, 2013 11:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3730 3730
Size: 6853
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2436 2612 4663 278
Size: 53 83 186 936 900 176 2051 2468
Fragments arranged by size:
2468 2051 936 900 186 176 83 53
AccI GT'mk_AC
Cuts at: 1937 4196 1937
Size: 2259 4594
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 4916 6300 6568 1632
Size: 572 2712 1384 268 1917
Fragments arranged by size:
2712 1917 1384 572 268
AfeI AGC'GCT
Cuts at: 3773 3773
Size: 6853
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6656
AflIII A'CryG_T
Cuts at: 1203 2595 5752 6729 1203
Size: 1392 3157 977 1327
Fragments arranged by size:
3157 1392 1327 977
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AlwNI CAG_nnn'CTG
Cuts at: 3163 3787 4596 6168 3163
Size: 624 809 1572 3848
Fragments arranged by size:
3848 1572 809 624
ApaI G_GGCC'C
Cuts at: 2091 2295 2341 2091
Size: 204 46 6603
Fragments arranged by size:
6603 204 46
ApaLI G'TGCA_C
Cuts at: 3485 4910 6066 3485
Size: 1425 1156 4272
Fragments arranged by size:
4272 1425 1156
ApoI r'AATT_y
Cuts at: 1493 3964 4162 4388 4540 6649 1493
Size: 2471 198 226 152 2109 1697
Fragments arranged by size:
2471 2109 1697 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 2263 2654 4178 1870
Size: 73 320 391 1524 4545
Fragments arranged by size:
4545 1524 391 320 73
BamHI G'GATC_C
Cuts at: 4183 4550 4183
Size: 367 6486
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2762 3169 4174 5629
Size: 325 217 627 321 654 407 1005 1455
Cuts at: 5629 618
Size: 1842
Fragments arranged by size:
1842 1455 1005 654 627 407 325 321
217
BanII G_rGCy'C
Cuts at: 1391 2091 2295 2341 2522 3060 3233 3325 4172
Size: 700 204 46 181 538 173 92 847
Cuts at: 4172 1391
Size: 4072
Fragments arranged by size:
4072 847 700 538 204 181 173 92
46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2056 2436 3117 3509 928
Size: 971 157 380 681 392 4272
Fragments arranged by size:
4272 971 681 392 380 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5044 5078 1903
Size: 34 50 34 3023 34 3678
Fragments arranged by size:
3678 3023 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4745 5955 4745
Size: 1210 5643
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6853
BfrBI ATG'CAT
Cuts at: 2147 2147
Size: 6853
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2817 2942 3359
Size: 107 122 71 631 605 1145 125 417
Cuts at: 3359 5464 136
Size: 2105 1525
Fragments arranged by size:
2105 1525 1145 631 605 417 125 122
107 71
BglII A'GATC_T
Cuts at: 6848 6848
Size: 6853
BlpI GC'TnA_GC
Cuts at: 3775 4270 3775
Size: 495 6358
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2295 2341 2767 3489 4914 6070 1790
Size: 301 204 46 426 722 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 722 426 301 204 46
BmgBI CAC'GTC
Cuts at: 1318 3706 4024 1318
Size: 2388 318 4147
Fragments arranged by size:
4147 2388 318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2914 4584 5542
Size: 416 53 83 399 24 1497 1670 958
Cuts at: 5542 442
Size: 1753
Fragments arranged by size:
1753 1670 1497 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2581 2389
Size: 192 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2510 2758 3044 3575 5513 1239
Size: 1130 141 248 286 531 1938 2579
Fragments arranged by size:
2579 1938 1130 531 286 248 141
Bpu10I CC'TnA_GC
Cuts at: 2516 3780 2516
Size: 1264 5589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3032 4978 5843 6141 3032
Size: 1946 865 298 3744
Fragments arranged by size:
3744 1946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2496 5516 882
Size: 617 997 3020 2219
Fragments arranged by size:
3020 2219 997 617
BsaAI yAC'GTr
Cuts at: 493 1204 4644 493
Size: 711 3440 2702
Fragments arranged by size:
3440 2702 711
BsaBI GATnn'nnATC
Cuts at: 6847 6847
Size: 6853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 2609 2994 3362
Size: 53 83 186 936 900 176 385 368
Cuts at: 3362 4660 5042 5677 275
Size: 1298 382 635 1451
Fragments arranged by size:
1451 1298 936 900 635 385 382 368
186 176 83 53
BsaWI w'CCGG_w
Cuts at: 3823 5286 5684 5958 6105 6516 3823
Size: 1463 398 274 147 411 4160
Fragments arranged by size:
4160 1463 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 2702 2732 2979
Size: 30 165 30 891 30 378 30 247
Cuts at: 2979 3009 1178
Size: 30 5022
Fragments arranged by size:
5022 891 378 247 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2302 2554 2672 2761 2762 3109 1186
Size: 902 214 252 118 89 1 347 4930
Fragments arranged by size:
4930 902 347 252 214 118 89 1
BseYI C'CCAG_C
Cuts at: 2483 2677 2695 2758 3716 3768 6056 6552 2483
Size: 194 18 63 958 52 2288 496 2784
Fragments arranged by size:
2784 2288 958 496 194 63 52 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2671 3320 3407 1163
Size: 573 935 649 87 4609
Fragments arranged by size:
4609 935 649 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2821 3096 3369 3454 3724 3828
Size: 275 186 820 275 273 85 270 104
Cuts at: 3828 4303 5064 6092 1540
Size: 475 761 1028 2301
Fragments arranged by size:
2301 1028 820 761 475 275 275 273
270 186 104 85
BsiHKAI G_wGCw'C
Cuts at: 3489 3812 4914 4999 6070 3489
Size: 323 1102 85 1071 4272
Fragments arranged by size:
4272 1102 1071 323 85
BsiWI C'GTAC_G
Cuts at: 2592 2592
Size: 6853
BsmI GAATG_Cn'
Cuts at: 3767 4364 4457 3767
Size: 597 93 6163
Fragments arranged by size:
6163 597 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3326 3647 3326
Size: 321 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2295 2341 2522 2767 3060 3233
Size: 399 301 204 46 181 245 293 173
Cuts at: 3233 3325 3489 3812 4172 4914 4999 6070 1391
Size: 92 164 323 360 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 323 301 293
245 204 181 173 164 92 85 46
BspEI T'CCGG_A
Cuts at: 6516 6516
Size: 6853
BspHI T'CATG_A
Cuts at: 4742 6472 4742
Size: 1730 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3730 4208 844
Size: 2886 478 3489
Fragments arranged by size:
3489 2886 478
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 2769 4740 1394
Size: 429 353 593 1971 3507
Fragments arranged by size:
3507 1971 593 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 5348 5522 65
Size: 1183 4100 174 1396
Fragments arranged by size:
4100 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2200 2248 2820 5497 5684 1700
Size: 110 292 98 48 572 2677 187 2869
Fragments arranged by size:
2869 2677 572 292 187 110 98 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 2098 1925
Size: 173 6680
BssSI C'ACGA_G
Cuts at: 2374 2509 2899 3524 4913 5925 2374
Size: 135 390 625 1389 1012 3302
Fragments arranged by size:
3302 1389 1012 625 390 135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 5657 5657
Size: 6853
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6853
BstXI CCAn_nnnn'nTGG
Cuts at: 3553 3553
Size: 6853
BstYI r'GATC_y
Cuts at: 1972 3558 3981 4183 4550 4935 4952 6404 6490
Size: 1586 423 202 367 385 17 1452 86
Cuts at: 6490 6502 6848 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1586 1452 423 385 367 346 202
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2181 2295 2463 2615 2646 2671 2705
Size: 552 1116 114 168 152 31 25 34
Cuts at: 2705 2876 513
Size: 171 4490
Fragments arranged by size:
4490 1116 552 171 168 152 114 34
31 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6114
Fragments arranged by size:
6114 683 56
BtsI GCAGTG_nn'
Cuts at: 2690 4369 5163 5183 2690
Size: 1679 794 20 4360
Fragments arranged by size:
4360 1679 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6818
DraI TTT'AAA
Cuts at: 4157 4157
Size: 6853
DraIII CAC_nnn'GTG
Cuts at: 2419 2419
Size: 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3881 5860 809
Size: 507 2565 1979 1802
Fragments arranged by size:
2565 1979 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 2466 2523
Size: 54 1425 50 275 18 487 149 57
Cuts at: 2523 2818 2934 2943 3093 3366 3721 3856 3910
Size: 295 116 9 150 273 355 135 54
Cuts at: 3910 4300 5189 6818 8
Size: 390 889 1629 43
Fragments arranged by size:
1629 1425 889 487 390 355 295 275
273 150 149 135 116 57 54 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2818 3093 3366 3721 4300 1537
Size: 275 1006 275 273 355 579 4090
Fragments arranged by size:
4090 1006 579 355 275 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 4043 4783 1353
Size: 2690 740 3423
Fragments arranged by size:
3423 2690 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 3461 5440 5814 5960 387
Size: 1064 138 678 1194 1979 374 146 1280
Fragments arranged by size:
1979 1280 1194 1064 678 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2369 2510 2758 3044 3575 4916
Size: 393 572 165 141 248 286 531 1341
Cuts at: 4916 5513 6300 6568 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1341 787 597 572 531 393 286
268 248 165 141
EcoO109I rG'GnC_Cy
Cuts at: 2087 2337 2338 2682 3070 3949 2087
Size: 250 1 344 388 879 4991
Fragments arranged by size:
4991 879 388 344 250 1
EcoRI G'AATT_C
Cuts at: 3964 4162 4540 3964
Size: 198 378 6277
Fragments arranged by size:
6277 378 198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 6853
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 3047 3775 6000 1678
Size: 1369 728 2225 2531
Fragments arranged by size:
2531 2225 1369 728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2979 3011 3104
Size: 32 135 32 674 32 701 32 93
Cuts at: 3104 3136 3366 3398 5494 5526 5568 5600 1373
Size: 32 230 32 2096 32 42 32 2626
Fragments arranged by size:
2626 2096 701 674 230 135 93 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4197 4443 669
Size: 988 281 2259 246 3079
Fragments arranged by size:
3079 2259 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6853
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 4452 4452
Size: 6853
MluI A'CGCG_T
Cuts at: 2595 6729 2595
Size: 4134 2719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 2601 3564 3654 3774 5967 6151 1305
Size: 873 423 963 90 120 2193 184 2007
Fragments arranged by size:
2193 2007 963 873 423 184 120 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3858 3912 10
Size: 54 1425 343 2026 54 2951
Fragments arranged by size:
2951 2026 1425 343 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3890 4811 5170 5329 518
Size: 648 2724 921 359 159 2042
Fragments arranged by size:
2724 2042 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2123 2297 2673 2707 2878 3259 3391 3418
Size: 517 174 376 34 171 381 132 27
Cuts at: 3418 3720 4269 4946 6094 6339 1606
Size: 302 549 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 517 381 376 302
245 174 171 132 34 27
NaeI GCC'GGC
Cuts at: 2104 2202 2250 2822 2104
Size: 98 48 572 6135
Fragments arranged by size:
6135 572 98 48
NcoI C'CATG_G
Cuts at: 513 1065 2615 513
Size: 552 1550 4751
Fragments arranged by size:
4751 1550 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6853
NgoMIV G'CCGG_C
Cuts at: 2102 2200 2248 2820 2102
Size: 98 48 572 6135
Fragments arranged by size:
6135 572 98 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 3721 4300 3721
Size: 579 6274
NsiI A_TGCA'T
Cuts at: 2149 2149
Size: 6853
NspI r_CATG'y
Cuts at: 1132 1229 3586 4211 5756 1132
Size: 97 2357 625 1545 2229
Fragments arranged by size:
2357 2229 1545 625 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6853
PciI A'CATG_T
Cuts at: 5752 5752
Size: 6853
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 3163 1108
Size: 1311 744 4798
Fragments arranged by size:
4798 1311 744
PfoI T'CCnGG_A
Cuts at: 2540 3552 2540
Size: 1012 5841
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4922 4954 6435 6467 4922
Size: 32 1481 32 5308
Fragments arranged by size:
5308 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3070 3949 3070
Size: 879 5974
PshAI GACnn'nnGTC
Cuts at: 1613 2782 1613
Size: 1169 5684
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PspOMI G'GGCC_C
Cuts at: 2087 2291 2337 2087
Size: 204 46 6603
Fragments arranged by size:
6603 204 46
PstI C_TGCA'G
Cuts at: 830 3429 3741 3873 4205 830
Size: 2599 312 132 332 3478
Fragments arranged by size:
3478 2599 332 312 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
PvuII CAG'CTG
Cuts at: 3259 3391 3259
Size: 132 6721
SacII CC_GC'GG
Cuts at: 2298 2674 2708 2879 2298
Size: 376 34 171 6272
Fragments arranged by size:
6272 376 171 34
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SbfI CC_TGCA'GG
Cuts at: 3741 3873 4205 3741
Size: 132 332 6389
Fragments arranged by size:
6389 332 132
ScaI AGT'ACT
Cuts at: 5101 6813 5101
Size: 1712 5141
SexAI A'CCwGG_T
Cuts at: 3480 3480
Size: 6853
SfcI C'TryA_G
Cuts at: 826 1045 2497 3425 3737 3869 4201 5336 6017
Size: 219 1452 928 312 132 332 1135 681
Cuts at: 6017 6208 826
Size: 191 1471
Fragments arranged by size:
1471 1452 1135 928 681 332 312 219
191 132
SfiI GGCCn_nnn'nGGCC
Cuts at: 2942 2942
Size: 6853
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 2102 2248 1700
Size: 402 146 6305
Fragments arranged by size:
6305 402 146
SmaI CCC'GGG
Cuts at: 1872 4180 1872
Size: 2308 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3047 4957 5858 6120 820
Size: 197 926 1104 1910 901 262 1553
Fragments arranged by size:
1910 1553 1104 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4644 493
Size: 4151 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 3586 4211 1132
Size: 2454 625 3774
Fragments arranged by size:
3774 2454 625
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 2329 2495 2561 1517
Size: 812 166 66 5809
Fragments arranged by size:
5809 812 166 66
StyI C'CwwG_G
Cuts at: 513 1065 2615 3879 4292 513
Size: 552 1550 1264 413 3074
Fragments arranged by size:
3074 1550 1264 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2750 2906 2933 4880 4897 5050 5235
Size: 1255 1170 156 27 1947 17 153 185
Cuts at: 5235 5680 325
Size: 445 1498
Fragments arranged by size:
1947 1498 1255 1170 445 185 156 153
27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2805 2922 5099
Size: 274 80 33 51 1443 828 117 2177
Cuts at: 5099 6811 96
Size: 1712 138
Fragments arranged by size:
2177 1712 1443 828 274 138 117 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2005 2037 2702 2734 4069 4101 6813 6845 2005
Size: 32 665 32 1335 32 2712 32 2013
Fragments arranged by size:
2712 2013 1335 665 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3508 1316
Size: 2192 4661
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6853
XmaI C'CCGG_G
Cuts at: 1870 4178 1870
Size: 2308 4545
XmnI GAAnn'nnTTC
Cuts at: 4982 4982
Size: 6853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2434 2610 4661 276
Size: 53 83 186 936 900 176 2051 2468
Fragments arranged by size:
2468 2051 936 900 186 176 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SfoI SrfI SwaI XcmI