Restriction map for
pFN21AE2482
(Circular) (Six-base) MAPSORT of: pFN21AE2482.seq Check: 8461 from: 1 to: 6109
pFN21AE2482 6109 bp
With 182 enzymes: SgfI *
September 20, 2013 17:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3009 3919 278
Size: 53 83 186 936 1473 910 2468
Fragments arranged by size:
2468 1473 936 910 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3263 3452 1937
Size: 1326 189 4594
Fragments arranged by size:
4594 1326 189
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3108 3360 4172 5556 5824 1632
Size: 1476 252 812 1384 268 1917
Fragments arranged by size:
1917 1476 1384 812 268 252
AflII C'TTAA_G
Cuts at: 820 1017 3268 820
Size: 197 2251 3661
Fragments arranged by size:
3661 2251 197
AflIII A'CryG_T
Cuts at: 1203 2105 3202 5008 5985 1203
Size: 902 1097 1806 977 1327
Fragments arranged by size:
1806 1327 1097 977 902
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2166 2823 1184
Size: 982 657 4470
Fragments arranged by size:
4470 982 657
AlwNI CAG_nnn'CTG
Cuts at: 3852 5424 3852
Size: 1572 4537
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2270 3053 3183 3380 3418 3644 3796 5905
Size: 777 783 130 197 38 226 152 2109
Cuts at: 5905 1493
Size: 1697
Fragments arranged by size:
2109 1697 783 777 226 197 152 130
38
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 3434 1870
Size: 73 313 1178 4545
Fragments arranged by size:
4545 1178 313 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4885 618
Size: 325 217 627 1643 1455 1842
Fragments arranged by size:
1842 1643 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2724 3428 1391
Size: 1333 704 4072
Fragments arranged by size:
4072 1333 704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3001 3036 928
Size: 971 1102 35 4001
Fragments arranged by size:
4001 1102 971 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 2890 1548
Size: 1342 4767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2061 6104 2061
Size: 4043 2066
BlpI GC'TnA_GC
Cuts at: 2376 3526 2376
Size: 1150 4959
Bme1580I G_kGCm'C
Cuts at: 1790 2460 4170 5326 1790
Size: 670 1710 1156 2573
Fragments arranged by size:
2573 1710 1156 670
BmgBI CAC'GTC
Cuts at: 1318 3205 1318
Size: 1887 4222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3840 4798 442
Size: 416 53 83 399 24 2423 958 1753
Fragments arranged by size:
2423 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3194 1056
Size: 2138 3971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2708 4769 1239
Size: 1149 320 2061 2579
Fragments arranged by size:
2579 2061 1149 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2731 4234 5099 5397 2731
Size: 1503 865 298 3443
Fragments arranged by size:
3443 1503 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4772 882
Size: 617 3273 2219
Fragments arranged by size:
3273 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2387 3006 3916 4298
Size: 53 83 186 936 854 619 910 382
Cuts at: 4298 4933 275
Size: 635 1451
Fragments arranged by size:
1451 936 910 854 635 619 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2328 4542 4940 5214 5361 5772 2328
Size: 2214 398 274 147 411 2665
Fragments arranged by size:
2665 2214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4477
Fragments arranged by size:
4477 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6109
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5536
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 4170 4255 5326 2724
Size: 1446 85 1071 3507
Fragments arranged by size:
3507 1446 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2460 2724 3428 4170 4255 5326 1391
Size: 399 670 264 704 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 670 399 264 85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2708 2903 3464 844
Size: 1864 195 561 3489
Fragments arranged by size:
3489 1864 561 195
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2155 3399 4604 4778 65
Size: 1183 907 1244 1205 174 1396
Fragments arranged by size:
1396 1244 1205 1183 907 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2061 3439 3806 4191 4208 5660 5746 5758
Size: 89 1378 367 385 17 1452 86 12
Cuts at: 5758 6104 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1378 385 367 346 89 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2466 3019 2466
Size: 553 5556
BtgI C'CryG_G
Cuts at: 513 1065 2646 513
Size: 552 1581 3976
Fragments arranged by size:
3976 1581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2042 487
Size: 683 56 816 4554
Fragments arranged by size:
4554 816 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 4419 4439 3625
Size: 794 20 5295
Fragments arranged by size:
5295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2047 2082 529
Size: 35 1483 35 4556
Fragments arranged by size:
4556 1483 35 35
DraI TTT'AAA
Cuts at: 3313 3413 3313
Size: 100 6009
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2489 5116 809
Size: 507 1173 2627 1802
Fragments arranged by size:
2627 1802 1173 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2406 3556
Size: 54 1425 50 275 18 445 131 1150
Cuts at: 3556 4445 6074 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1150 889 445 275 131 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2712 3201 4039 1353
Size: 1359 489 838 3423
Fragments arranged by size:
3423 1359 838 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2476 4696 5070 5216 387
Size: 1064 138 887 2220 374 146 1280
Fragments arranged by size:
2220 1280 1064 887 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2708 3108 3360 4172 4769 5556
Size: 393 756 320 400 252 812 597 787
Cuts at: 5556 5824 1239
Size: 268 1524
Fragments arranged by size:
1524 812 787 756 597 400 393 320
268 252
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 5256 1678
Size: 3578 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2063 2095 4750
Size: 32 135 32 448 32 11 32 2655
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2655 2626 448 135 42 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 2801 3708 2801
Size: 907 5202
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5223 5407 1305
Size: 3918 184 2007
Fragments arranged by size:
3918 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 4067 4426 4585 518
Size: 648 928 1973 359 159 2042
Fragments arranged by size:
2042 1973 928 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2211 2383 2785 3525 4202 5350 5595 1606
Size: 605 172 402 740 677 1148 245 2120
Fragments arranged by size:
2120 1148 740 677 605 402 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2646 513
Size: 552 1581 3976
Fragments arranged by size:
3976 1581 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 3190 1052
Size: 2138 3971
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 2109 3467 5012 1132
Size: 97 880 1358 1545 2229
Fragments arranged by size:
2229 1545 1358 880 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 2105 5008 2105
Size: 2903 3206
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3087 4178 4210 5691 5723 3055
Size: 32 1091 32 1481 32 3441
Fragments arranged by size:
3441 1481 1091 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SapI GCTCTTCn'nnn_
Cuts at: 2712 3201 2712
Size: 489 5620
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 3209 3457 4592 5273 5464 826
Size: 219 2164 248 1135 681 191 1471
Fragments arranged by size:
2164 1471 1135 681 248 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2256 2710 3268 4213 5114 5376
Size: 197 926 313 454 558 945 901 262
Cuts at: 5376 820
Size: 1553
Fragments arranged by size:
1553 945 926 901 558 454 313 262
197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2646 3548 513
Size: 552 1581 902 3074
Fragments arranged by size:
3074 1581 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4136 4153 4306 4491 4936 325
Size: 1255 2556 17 153 185 445 1498
Fragments arranged by size:
2556 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2317 2976 3118
Size: 274 80 33 51 1443 340 659 142
Cuts at: 3118 4355 6067 96
Size: 1237 1712 138
Fragments arranged by size:
1712 1443 1237 659 340 274 142 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 6069 6101 2780
Size: 32 3257 32 2788
Fragments arranged by size:
3257 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 2920 3445 2920
Size: 525 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2363 2363
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 2256 1943
Size: 313 5796
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 2049 4238 2049
Size: 2189 3920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3007 3917 276
Size: 53 83 186 936 1473 910 2468
Fragments arranged by size:
2468 1473 936 910 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI