Restriction map for
pFN21AE2480
(Circular) (Six-base) MAPSORT of: pFN21AE2480.seq Check: 5593 from: 1 to: 9064
pFN21AE2480 9064 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5636 5636
Size: 9064
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6874 278
Size: 53 83 186 936 5338 2468
Fragments arranged by size:
5338 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 5695 6407 1937
Size: 960 2798 712 4594
Fragments arranged by size:
4594 2798 960 712
Acc65I G'GTAC_C
Cuts at: 6385 6385
Size: 9064
AclI AA'CG_TT
Cuts at: 1563 7191 7564 1563
Size: 5628 373 3063
Fragments arranged by size:
5628 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 2585 2748 3122 3699 4400 4583 4664
Size: 501 452 163 374 577 701 183 81
Cuts at: 4664 5127 5402 5649 7127 8511 8779 1632
Size: 463 275 247 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 701 577 501 463 452
374 275 268 247 183 163 81
AfeI AGC'GCT
Cuts at: 2428 2428
Size: 9064
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8867
AflIII A'CryG_T
Cuts at: 1203 3911 3941 4168 5118 5120 7963 8940 1203
Size: 2708 30 227 950 2 2843 977 1327
Fragments arranged by size:
2843 2708 1327 977 950 227 30 2
AgeI A'CCGG_T
Cuts at: 2892 4943 7895 2892
Size: 2051 2952 4061
Fragments arranged by size:
4061 2952 2051
AhdI GACnn_n'nnGTC
Cuts at: 3549 7793 3549
Size: 4244 4820
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9064
AlwNI CAG_nnn'CTG
Cuts at: 3446 3697 4823 6331 6807 8379 3446
Size: 251 1126 1508 476 1572 4131
Fragments arranged by size:
4131 1572 1508 1126 476 251
ApaLI G'TGCA_C
Cuts at: 7121 8277 7121
Size: 1156 7908
ApoI r'AATT_y
Cuts at: 1493 2328 2643 2875 2990 3095 3642 4705 5058
Size: 835 315 232 115 105 547 1063 353
Cuts at: 5058 5517 5867 5993 6373 6599 6751 8860 1493
Size: 459 350 126 380 226 152 2109 1697
Fragments arranged by size:
2109 1697 1063 835 547 459 380 353
350 315 232 226 152 126 115 105
AseI AT'TA_AT
Cuts at: 160 1032 2928 4202 7618 160
Size: 872 1896 1274 3416 1606
Fragments arranged by size:
3416 1896 1606 1274 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9064
AvaI C'yCGr_G
Cuts at: 1870 1943 5346 6389 1870
Size: 73 3403 1043 4545
Fragments arranged by size:
4545 3403 1043 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4302 4335 4302
Size: 33 9031
BamHI G'GATC_C
Cuts at: 6394 6761 6394
Size: 367 8697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2889 6385 7840 618
Size: 325 217 627 1102 3496 1455 1842
Fragments arranged by size:
3496 1842 1455 1102 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5347 6383 1391
Size: 3956 1036 4072
Fragments arranged by size:
4072 3956 1036
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2876 4346 5763 928
Size: 971 977 1470 1417 4229
Fragments arranged by size:
4229 1470 1417 977 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7255 7289 1903
Size: 34 5318 34 3678
Fragments arranged by size:
5318 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4303 5544 6956 8166 4303
Size: 1241 1412 1210 5201
Fragments arranged by size:
5201 1412 1241 1210
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 8605
BfrBI ATG'CAT
Cuts at: 2487 3317 2487
Size: 830 8234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7675 136
Size: 107 122 71 631 605 6003 1525
Fragments arranged by size:
6003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5854 5963 9059 5854
Size: 109 3096 5859
Fragments arranged by size:
5859 3096 109
BlpI GC'TnA_GC
Cuts at: 4238 5506 6481 4238
Size: 1268 975 6821
Fragments arranged by size:
6821 1268 975
Bme1580I G_kGCm'C
Cuts at: 1790 3919 7125 8281 1790
Size: 2129 3206 1156 2573
Fragments arranged by size:
3206 2573 2129 1156
BmgBI CAC'GTC
Cuts at: 1318 3944 1318
Size: 2626 6438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3010 5001 6795
Size: 416 53 83 399 24 1593 1991 1794
Cuts at: 6795 7753 442
Size: 958 1753
Fragments arranged by size:
1991 1794 1753 1593 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 3468 3945 5564 5723 6005 6032 7724
Size: 1283 946 477 1619 159 282 27 1692
Cuts at: 7724 1239
Size: 2579
Fragments arranged by size:
2579 1692 1619 1283 946 477 282 159
27
Bpu10I CC'TnA_GC
Cuts at: 6119 6119
Size: 9064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 4622 5297 7189 8054 8352 4264
Size: 358 675 1892 865 298 4976
Fragments arranged by size:
4976 1892 865 675 358 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5292 6187 7727 882
Size: 617 3793 895 1540 2219
Fragments arranged by size:
3793 2219 1540 895 617
BsaAI yAC'GTr
Cuts at: 493 1204 3914 5121 6855 493
Size: 711 2710 1207 1734 2702
Fragments arranged by size:
2710 2702 1734 1207 711
BsaBI GATnn'nnATC
Cuts at: 9058 9058
Size: 9064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6292 6871 7253 7888
Size: 53 83 186 936 4759 579 382 635
Cuts at: 7888 275
Size: 1451
Fragments arranged by size:
4759 1451 936 635 579 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2892 4943 5026 6029 7497 7895 8169 8316 8727
Size: 2051 83 1003 1468 398 274 147 411
Cuts at: 8727 2892
Size: 3229
Fragments arranged by size:
3229 2051 1468 1003 411 398 274 147
83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8839
Fragments arranged by size:
8839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2298 3178 3575 1186
Size: 1112 880 397 6675
Fragments arranged by size:
6675 1112 880 397
BseYI C'CCAG_C
Cuts at: 2429 8267 8763 2429
Size: 5838 496 2730
Fragments arranged by size:
5838 2730 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 4759 1163
Size: 573 1218 1805 5468
Fragments arranged by size:
5468 1805 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6514 7275 8303 1540
Size: 275 186 4513 761 1028 2301
Fragments arranged by size:
4513 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3736 5347 5621 6187 7125 7210 8281 3736
Size: 1611 274 566 938 85 1071 4519
Fragments arranged by size:
4519 1611 1071 938 566 274 85
BsmI GAATG_Cn'
Cuts at: 3021 6575 6668 3021
Size: 3554 93 5417
Fragments arranged by size:
5417 3554 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6299 6299
Size: 9064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3736 3919 5347 5621 6187 6383 7125
Size: 399 1946 183 1428 274 566 196 742
Cuts at: 7125 7210 8281 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1946 1428 1071 742 566 399 274
196 183 85
BspEI T'CCGG_A
Cuts at: 8727 8727
Size: 9064
BspHI T'CATG_A
Cuts at: 6953 8683 6953
Size: 1730 7334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5563 5636 5664 6419 844
Size: 4719 73 28 755 3489
Fragments arranged by size:
4719 3489 755 73 28
BsrBI CCG'CTC
Cuts at: 1394 1823 6951 1394
Size: 429 5128 3507
Fragments arranged by size:
5128 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5205 6047 7559 7733 65
Size: 1183 3957 842 1512 174 1396
Fragments arranged by size:
3957 1512 1396 1183 842 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2892 4943 7708 7895 1700
Size: 110 1082 2051 2765 187 2869
Fragments arranged by size:
2869 2765 2051 1082 187 110
BsrGI T'GTAC_A
Cuts at: 96 3324 96
Size: 3228 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9064
BssSI C'ACGA_G
Cuts at: 5660 7124 8136 5660
Size: 1464 1012 6588
Fragments arranged by size:
6588 1464 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2303 4737 6331 6343 794
Size: 1509 2434 1594 12 3515
Fragments arranged by size:
3515 2434 1594 1509 12
BstBI TT'CG_AA
Cuts at: 7868 7868
Size: 9064
BstEII G'GTnAC_C
Cuts at: 1184 5045 1184
Size: 3861 5203
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 9064
BstYI r'GATC_y
Cuts at: 1972 3574 4259 5854 5963 6394 6761 7146 7163
Size: 1602 685 1595 109 431 367 385 17
Cuts at: 7163 8615 8701 8713 9059 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1602 1595 1452 685 431 385 367
346 109 86 17 12
BstZ17I GTA'TAC
Cuts at: 5696 5696
Size: 9064
BtgI C'CryG_G
Cuts at: 513 1065 2592 6091 513
Size: 552 1527 3499 3486
Fragments arranged by size:
3499 3486 1527 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8325
Fragments arranged by size:
8325 683 56
BtsI GCAGTG_nn'
Cuts at: 2942 4747 5494 6580 7374 7394 2942
Size: 1805 747 1086 794 20 4612
Fragments arranged by size:
4612 1805 1086 794 747 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2106 2141 529
Size: 35 1542 35 7452
Fragments arranged by size:
7452 1542 35 35
DraI TTT'AAA
Cuts at: 2689 3048 3667 3886 6368 2689
Size: 359 619 219 2482 5385
Fragments arranged by size:
5385 2482 619 359 219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8071 809
Size: 507 6755 1802
Fragments arranged by size:
6755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2313 5491
Size: 54 1425 50 275 18 225 258 3178
Cuts at: 5491 6511 7400 9029 8
Size: 1020 889 1629 43
Fragments arranged by size:
3178 1629 1425 1020 889 275 258 225
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6511 1537
Size: 275 4699 4090
Fragments arranged by size:
4699 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3141 5103 5625 6994 1353
Size: 1788 1962 522 1369 3423
Fragments arranged by size:
3423 1962 1788 1369 522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7651 8025 8171 387
Size: 1064 138 6062 374 146 1280
Fragments arranged by size:
6062 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5345 5345
Size: 9064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2522 2585 2748 3122 3468 3699
Size: 393 501 389 63 163 374 346 231
Cuts at: 3699 3945 4400 4583 4664 5127 5402 5564 5649
Size: 246 455 183 81 463 275 162 85
Cuts at: 5649 5723 6005 6032 7127 7724 8511 8779 1239
Size: 74 282 27 1095 597 787 268 1524
Fragments arranged by size:
1524 1095 787 597 501 463 455 393
389 374 346 282 275 268 246 231
183 163 162 85 81 74 63 27
EcoRI G'AATT_C
Cuts at: 2875 3642 6373 6751 2875
Size: 767 2731 378 5188
Fragments arranged by size:
5188 2731 767 378
EcoRV GAT'ATC
Cuts at: 4899 4899
Size: 9064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4258 4411 4258
Size: 153 8911
FspI TGC'GCA
Cuts at: 7570 7570
Size: 9064
HaeII r_GCGC'y
Cuts at: 1678 2430 8211 1678
Size: 752 5781 2531
Fragments arranged by size:
5781 2531 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3535 3567 4534 4566 4595
Size: 32 135 32 1963 32 967 32 29
Cuts at: 4595 4627 4669 4701 4978 5010 5270 5302 7705
Size: 32 42 32 277 32 260 32 2403
Cuts at: 7705 7737 7779 7811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2403 1963 967 277 260 135 42
42 32 32 32 32 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 4460 4749 5179 5303 6408 6654
Size: 988 281 2522 289 430 124 1105 246
Cuts at: 6654 669
Size: 3079
Fragments arranged by size:
3079 2522 1105 988 430 289 281 246
124
HindIII A'AGCT_T
Cuts at: 748 2397 4240 748
Size: 1649 1843 5572
Fragments arranged by size:
5572 1843 1649
HpaI GTT'AAC
Cuts at: 4749 6654 4749
Size: 1905 7159
KpnI G_GTAC'C
Cuts at: 6389 6389
Size: 9064
MfeI C'AATT_G
Cuts at: 3429 3769 4007 6663 3429
Size: 340 238 2656 5830
Fragments arranged by size:
5830 2656 340 238
MluI A'CGCG_T
Cuts at: 8940 8940
Size: 9064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5735 8178 8362 1305
Size: 4430 2443 184 2007
Fragments arranged by size:
4430 2443 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2315 5493 10
Size: 54 1425 343 225 258 3178 3581
Fragments arranged by size:
3581 3178 1425 343 258 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4427 5148 6178 7022 7381 7540 518
Size: 648 3261 721 1030 844 359 159 2042
Fragments arranged by size:
3261 2042 1030 844 721 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 5140 6169 6480 7157 8305 8550 1606
Size: 3534 1029 311 677 1148 245 2120
Fragments arranged by size:
3534 2120 1148 1029 677 311 245
NcoI C'CATG_G
Cuts at: 513 1065 2592 6091 513
Size: 552 1527 3499 3486
Fragments arranged by size:
3499 3486 1527 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9064
NotI GC'GGCC_GC
Cuts at: 6511 6511
Size: 9064
NsiI A_TGCA'T
Cuts at: 2489 3319 2489
Size: 830 8234
NspI r_CATG'y
Cuts at: 1132 1229 3186 6422 7967 1132
Size: 97 1957 3236 1545 2229
Fragments arranged by size:
3236 2229 1957 1545 97
PasI CC'CwG_GG
Cuts at: 1348 5158 1348
Size: 3810 5254
PciI A'CATG_T
Cuts at: 7963 7963
Size: 9064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2852 1108
Size: 1744 7320
PfoI T'CCnGG_A
Cuts at: 3174 3477 3174
Size: 303 8761
PmeI GTTT'AAAC
Cuts at: 6368 6368
Size: 9064
PmlI CAC'GTG
Cuts at: 3914 5121 3914
Size: 1207 7857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2111 7133 7165 8646 8678 2079
Size: 32 5022 32 1481 32 2465
Fragments arranged by size:
5022 2465 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9064
PsiI TTA'TAA
Cuts at: 3830 6634 3830
Size: 2804 6260
PspXI vC'TCGA_Gb
Cuts at: 5346 5346
Size: 9064
PstI C_TGCA'G
Cuts at: 830 3256 6336 6416 830
Size: 2426 3080 80 3478
Fragments arranged by size:
3478 3080 2426 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9064
PvuII CAG'CTG
Cuts at: 6169 6169
Size: 9064
SacI G_AGCT'C
Cuts at: 5347 5347
Size: 9064
SalI G'TCGA_C
Cuts at: 1936 6406 1936
Size: 4470 4594
SbfI CC_TGCA'GG
Cuts at: 6416 6416
Size: 9064
ScaI AGT'ACT
Cuts at: 3598 7312 9024 3598
Size: 3714 1712 3638
Fragments arranged by size:
3714 3638 1712
SexAI A'CCwGG_T
Cuts at: 3901 3901
Size: 9064
SfcI C'TryA_G
Cuts at: 826 1045 3252 4065 6332 6412 7547 8228 8419
Size: 219 2207 813 2267 80 1135 681 191
Cuts at: 8419 826
Size: 1471
Fragments arranged by size:
2267 2207 1471 1135 813 681 219 191
80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9064
SmaI CCC'GGG
Cuts at: 1872 6391 1872
Size: 4519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 4601 5276 5346 7168 8069
Size: 197 926 2300 358 675 70 1822 901
Cuts at: 8069 8331 820
Size: 262 1553
Fragments arranged by size:
2300 1822 1553 926 901 675 358 262
197 70
SnaBI TAC'GTA
Cuts at: 493 6855 493
Size: 6362 2702
SpeI A'CTAG_T
Cuts at: 152 2776 5885 152
Size: 2624 3109 3331
Fragments arranged by size:
3331 3109 2624
SphI G_CATG'C
Cuts at: 1132 6422 1132
Size: 5290 3774
SspI AAT'ATT
Cuts at: 5 52 2302 3193 6988 5
Size: 47 2250 891 3795 2081
Fragments arranged by size:
3795 2250 2081 891 47
StuI AGG'CCT
Cuts at: 1517 2291 6287 1517
Size: 774 3996 4294
Fragments arranged by size:
4294 3996 774
StyI C'CwwG_G
Cuts at: 513 1065 2067 2264 2292 2592 4503 6040 6091
Size: 552 1002 197 28 300 1911 1537 51
Cuts at: 6091 6503 513
Size: 412 3074
Fragments arranged by size:
3074 1911 1537 1002 552 412 300 197
51 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7091 7108 7261 7446 7891 325
Size: 1255 5511 17 153 185 445 1498
Fragments arranged by size:
5511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2029 3324 3596
Size: 274 80 33 51 1443 52 1295 272
Cuts at: 3596 3724 3841 5237 5272 5292 7310 9022 96
Size: 128 117 1396 35 20 2018 1712 138
Fragments arranged by size:
2018 1712 1443 1396 1295 274 272 138
128 117 80 52 51 35 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 6045 6077 9024 9056 2176
Size: 32 3837 32 2947 32 2184
Fragments arranged by size:
3837 2947 2184 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3903 1316
Size: 2587 6477
XbaI T'CTAG_A
Cuts at: 3577 6400 3577
Size: 2823 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 3926 5983 6089 2065
Size: 1861 2057 106 5040
Fragments arranged by size:
5040 2057 1861 106
XhoI C'TCGA_G
Cuts at: 1943 5346 1943
Size: 3403 5661
XmaI C'CCGG_G
Cuts at: 1870 6389 1870
Size: 4519 4545
XmnI GAAnn'nnTTC
Cuts at: 5415 7193 5415
Size: 1778 7286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6872 276
Size: 53 83 186 936 5338 2468
Fragments arranged by size:
5338 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI
Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PpuMI PspOMI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI