Restriction map for
pFN21AE2474
(Circular) (Six-base) MAPSORT of: pFN21AE2474.seq Check: 4242 from: 1 to: 8704
pFN21AE2474 8704 bp
With 182 enzymes: SgfI *
June 25, 2013 12:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6514 278
Size: 53 83 186 936 4978 2468
Fragments arranged by size:
4978 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4301 6047 1937
Size: 2364 1746 4594
Fragments arranged by size:
4594 2364 1746
Acc65I G'GTAC_C
Cuts at: 6025 6025
Size: 8704
AclI AA'CG_TT
Cuts at: 1563 6831 7204 1563
Size: 5268 373 3063
Fragments arranged by size:
5268 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4163 4582 5449 5508 6767 8151 8419
Size: 1928 603 419 867 59 1259 1384 268
Cuts at: 8419 1632
Size: 1917
Fragments arranged by size:
1928 1917 1384 1259 867 603 419 268
59
AfeI AGC'GCT
Cuts at: 5254 5254
Size: 8704
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8507
AflIII A'CryG_T
Cuts at: 1203 3459 7603 8580 1203
Size: 2256 4144 977 1327
Fragments arranged by size:
4144 2256 1327 977
AgeI A'CCGG_T
Cuts at: 7535 7535
Size: 8704
AhdI GACnn_n'nnGTC
Cuts at: 5273 7433 5273
Size: 2160 6544
AleI CACnn'nnGTG
Cuts at: 1166 4427 4439 5670 1166
Size: 3261 12 1231 4200
Fragments arranged by size:
4200 3261 1231 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2180 2268 1184
Size: 996 88 7620
Fragments arranged by size:
7620 996 88
AlwNI CAG_nnn'CTG
Cuts at: 3358 3583 4908 6447 8019 3358
Size: 225 1325 1539 1572 4043
Fragments arranged by size:
4043 1572 1539 1325 225
ApaI G_GGCC'C
Cuts at: 3847 4758 3847
Size: 911 7793
ApaLI G'TGCA_C
Cuts at: 3529 6761 7917 3529
Size: 3232 1156 4316
Fragments arranged by size:
4316 3232 1156
ApoI r'AATT_y
Cuts at: 1493 2866 3948 6013 6239 6391 8500 1493
Size: 1373 1082 2065 226 152 2109 1697
Fragments arranged by size:
2109 2065 1697 1373 1082 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7258 160
Size: 872 6226 1606
Fragments arranged by size:
6226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8704
AvaI C'yCGr_G
Cuts at: 1870 1943 2399 3840 5651 5850 6029 1870
Size: 73 456 1441 1811 199 179 4545
Fragments arranged by size:
4545 1811 1441 456 199 179 73
BamHI G'GATC_C
Cuts at: 2617 2853 6034 6401 2617
Size: 236 3181 367 4920
Fragments arranged by size:
4920 3181 367 236
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3137 3694 6025 7480 618
Size: 325 217 627 1350 557 2331 1455 1842
Fragments arranged by size:
2331 1842 1455 1350 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 2406 2634 3203 3847 4293 4758 5065 5167
Size: 1015 228 569 644 446 465 307 102
Cuts at: 5167 5857 6023 1391
Size: 690 166 4072
Fragments arranged by size:
4072 1015 690 644 569 465 446 307
228 166 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3609 4325 4568 5355 928
Size: 971 1710 716 243 787 4277
Fragments arranged by size:
4277 1710 971 787 716 243
BbvCI CC'TCA_GC
Cuts at: 2654 3542 2654
Size: 888 7816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6895 6929 1903
Size: 34 4958 34 3678
Fragments arranged by size:
4958 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2706 6596 7806 2706
Size: 3890 1210 3604
Fragments arranged by size:
3890 3604 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8704
BfrBI ATG'CAT
Cuts at: 4482 5771 4482
Size: 1289 7415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2225 3110 7315
Size: 107 122 71 631 605 553 885 4205
Cuts at: 7315 136
Size: 1525
Fragments arranged by size:
4205 1525 885 631 605 553 122 107
71
BglII A'GATC_T
Cuts at: 8699 8699
Size: 8704
BlpI GC'TnA_GC
Cuts at: 2754 2955 3197 5856 6121 2754
Size: 201 242 2659 265 5337
Fragments arranged by size:
5337 2659 265 242 201
Bme1580I G_kGCm'C
Cuts at: 1790 3533 3699 3847 4697 4758 5223 6765 7921
Size: 1743 166 148 850 61 465 1542 1156
Cuts at: 7921 1790
Size: 2573
Fragments arranged by size:
2573 1743 1542 1156 850 465 166 148
61
BmgBI CAC'GTC
Cuts at: 1318 2709 3460 1318
Size: 1391 751 6562
Fragments arranged by size:
6562 1391 751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2332 4575 4707
Size: 416 53 83 399 24 915 2243 132
Cuts at: 4707 5328 5362 5406 6435 7393 442
Size: 621 34 44 1029 958 1753
Fragments arranged by size:
2243 1753 1029 958 915 621 416 399
132 83 53 44 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8704
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3274 5458 3274
Size: 2184 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 3017 4259 5542 7364 1239
Size: 1220 558 1242 1283 1822 2579
Fragments arranged by size:
2579 1822 1283 1242 1220 558
Bpu10I CC'TnA_GC
Cuts at: 2654 2859 2918 3542 4137 2654
Size: 205 59 624 595 7221
Fragments arranged by size:
7221 624 595 205 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 2732 3389 4604 4904 6829 7694 7992 2714
Size: 18 657 1215 300 1925 865 298 3426
Fragments arranged by size:
3426 1925 1215 865 657 300 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 7367 882
Size: 617 2907 2961 2219
Fragments arranged by size:
2961 2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6495 493
Size: 711 5291 2702
Fragments arranged by size:
5291 2702 711
BsaBI GATnn'nnATC
Cuts at: 5192 8698 5192
Size: 3506 5198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2766 3041 6511 6893
Size: 53 83 186 936 1233 275 3470 382
Cuts at: 6893 7528 275
Size: 635 1451
Fragments arranged by size:
3470 1451 1233 936 635 382 275 186
83 53
BsaWI w'CCGG_w
Cuts at: 2388 2484 3525 4362 7137 7535 7809 7956 8367
Size: 96 1041 837 2775 398 274 147 411
Cuts at: 8367 2388
Size: 2725
Fragments arranged by size:
2775 2725 1041 837 411 398 274 147
96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2457 2487 2879 2909 3236
Size: 30 165 30 1054 30 392 30 327
Cuts at: 3236 3266 4709 4739 1178
Size: 30 1443 30 5143
Fragments arranged by size:
5143 1443 1054 392 327 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2532 2615 2661 3221 3458 3494 3728
Size: 1243 103 83 46 560 237 36 234
Cuts at: 3728 4407 4838 5648 1186
Size: 679 431 810 4242
Fragments arranged by size:
4242 1243 810 679 560 431 237 234
103 83 46 36
BseYI C'CCAG_C
Cuts at: 2845 3017 3583 3667 4908 7907 8403 2845
Size: 172 566 84 1241 2999 496 3146
Fragments arranged by size:
3146 2999 1241 566 496 172 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 2335 2437 3486 4234 5648 5865
Size: 573 591 8 102 1049 748 1414 217
Cuts at: 5865 1163
Size: 4002
Fragments arranged by size:
4002 1414 1049 748 591 573 217 102
8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4468 6154 6915 7943 1540
Size: 275 186 2467 1686 761 1028 2301
Fragments arranged by size:
2467 2301 1686 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3533 4230 4293 4424 5167 5857 6765 6850 7921
Size: 697 63 131 743 690 908 85 1071
Cuts at: 7921 3533
Size: 4316
Fragments arranged by size:
4316 1071 908 743 697 690 131 85
63
BsmI GAATG_Cn'
Cuts at: 2431 3263 4922 6215 6308 2431
Size: 832 1659 1293 93 4827
Fragments arranged by size:
4827 1659 1293 832 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2634 3203 3533 3699 3847 4230
Size: 399 616 228 569 330 166 148 383
Cuts at: 4230 4293 4424 4697 4758 5065 5167 5223 5857
Size: 63 131 273 61 307 102 56 634
Cuts at: 5857 6023 6765 6850 7921 1391
Size: 166 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 616 569 399 383
330 307 273 228 166 166 148 131
102 85 63 61 56
BspEI T'CCGG_A
Cuts at: 2388 2484 8367 2388
Size: 96 5883 2725
Fragments arranged by size:
5883 2725 96
BspHI T'CATG_A
Cuts at: 2367 4557 6593 8323 2367
Size: 2190 2036 1730 2748
Fragments arranged by size:
2748 2190 2036 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6059 844
Size: 5215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6591 1394
Size: 429 4768 3507
Fragments arranged by size:
4768 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 4254 4609 4770 5932 7199 7373
Size: 1183 2046 960 355 161 1162 1267 174
Cuts at: 7373 65
Size: 1396
Fragments arranged by size:
2046 1396 1267 1183 1162 960 355 174
161
BsrFI r'CCGG_y
Cuts at: 1700 1810 3729 4463 7348 7535 1700
Size: 110 1919 734 2885 187 2869
Fragments arranged by size:
2885 2869 1919 734 187 110
BsrGI T'GTAC_A
Cuts at: 96 2376 4845 5076 96
Size: 2280 2469 231 3724
Fragments arranged by size:
3724 2469 2280 231
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8704
BssSI C'ACGA_G
Cuts at: 6764 7776 6764
Size: 1012 7692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2305 5931 794
Size: 1511 3626 3567
Fragments arranged by size:
3626 3567 1511
BstBI TT'CG_AA
Cuts at: 7508 7508
Size: 8704
BstEII G'GTnAC_C
Cuts at: 1184 3304 3829 1184
Size: 2120 525 6059
Fragments arranged by size:
6059 2120 525
BstXI CCAn_nnnn'nTGG
Cuts at: 4441 5615 4441
Size: 1174 7530
BstYI r'GATC_y
Cuts at: 1972 2065 2617 2853 3228 4621 5691 5919 6034
Size: 93 552 236 375 1393 1070 228 115
Cuts at: 6034 6401 6786 6803 8255 8341 8353 8699 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1393 1070 552 385 375 367
346 236 228 115 93 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2329 2409 4434 5104 5235 5671 513
Size: 552 1264 80 2025 670 131 436 3546
Fragments arranged by size:
3546 2025 1264 670 552 436 131 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4492 487
Size: 683 56 3266 4699
Fragments arranged by size:
4699 3266 683 56
BtsI GCAGTG_nn'
Cuts at: 2262 2381 3063 4246 4446 4806 6220 7014 7034
Size: 119 682 1183 200 360 1414 794 20
Cuts at: 7034 2262
Size: 3932
Fragments arranged by size:
3932 1414 1183 794 682 360 200 119
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5002 5037 529
Size: 35 4438 35 4196
Fragments arranged by size:
4438 4196 35 35
DraI TTT'AAA
Cuts at: 6008 6008
Size: 8704
DraIII CAC_nnn'GTG
Cuts at: 5955 5955
Size: 8704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2567 3127 7711 809
Size: 507 1251 560 4584 1802
Fragments arranged by size:
4584 1802 1251 560 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3102 3381 4219
Size: 54 1425 50 275 18 1272 279 838
Cuts at: 4219 4465 4536 5154 6151 7040 8669 8
Size: 246 71 618 997 889 1629 43
Fragments arranged by size:
1629 1425 1272 997 889 838 618 279
275 246 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4465 6151 1537
Size: 275 2653 1686 4090
Fragments arranged by size:
4090 2653 1686 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3689 4376 4943 5627 5804 5891 6634 1353
Size: 2336 687 567 684 177 87 743 3423
Fragments arranged by size:
3423 2336 743 687 684 567 177 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7291 7665 7811 387
Size: 1064 138 5702 374 146 1280
Fragments arranged by size:
5702 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4291 5165 5855 4291
Size: 874 690 7140
Fragments arranged by size:
7140 874 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 3017 3560 4163 4259 4582 5449
Size: 393 827 558 543 603 96 323 867
Cuts at: 5449 5508 5542 6767 7364 8151 8419 1239
Size: 59 34 1225 597 787 268 1524
Fragments arranged by size:
1524 1225 867 827 787 603 597 558
543 393 323 268 96 59 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2494 3238 4166 2494
Size: 744 928 7032
Fragments arranged by size:
7032 928 744
EcoO109I rG'GnC_Cy
Cuts at: 2992 4159 4754 4755 4858 5611 2992
Size: 1167 595 1 103 753 6085
Fragments arranged by size:
6085 1167 753 595 103 1
EcoRI G'AATT_C
Cuts at: 2866 6013 6391 2866
Size: 3147 378 5179
Fragments arranged by size:
5179 3147 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4570 5902 4570
Size: 1332 7372
FseI GG_CCGG'CC
Cuts at: 3733 3733
Size: 8704
FspI TGC'GCA
Cuts at: 7210 7210
Size: 8704
HaeII r_GCGC'y
Cuts at: 1678 5256 7851 1678
Size: 3578 2595 2531
Fragments arranged by size:
3578 2595 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 2457 2489 2879
Size: 32 135 32 712 32 141 32 390
Cuts at: 2879 2911 3042 3074 5259 5291 5540 5572 7345
Size: 32 131 32 2185 32 249 32 1773
Cuts at: 7345 7377 7419 7451 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2185 1773 712 390 249 141 135
131 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4960 6048 6294 669
Size: 988 281 3022 1088 246 3079
Fragments arranged by size:
3079 3022 1088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2957 4880 5029 748
Size: 2209 1923 149 4423
Fragments arranged by size:
4423 2209 1923 149
HpaI GTT'AAC
Cuts at: 6294 6294
Size: 8704
KpnI G_GTAC'C
Cuts at: 6029 6029
Size: 8704
MfeI C'AATT_G
Cuts at: 4098 6303 4098
Size: 2205 6499
MluI A'CGCG_T
Cuts at: 8580 8580
Size: 8704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 3028 3105 4752 4964 5764 7818 8002
Size: 1340 383 77 1647 212 800 2054 184
Cuts at: 8002 1305
Size: 2007
Fragments arranged by size:
2054 2007 1647 1340 800 383 212 184
77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 3383 4221 4538 5156
Size: 54 1425 343 1272 279 838 317 618
Cuts at: 5156 10
Size: 3558
Fragments arranged by size:
3558 1425 1272 838 618 343 317 279
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2366 2372 2804 4427 4439 5670
Size: 648 996 204 6 432 1623 12 1231
Cuts at: 5670 6662 7021 7180 518
Size: 992 359 159 2042
Fragments arranged by size:
2042 1623 1231 996 992 648 432 359
204 159 12 6
MspA1I CmG'CkG
Cuts at: 1606 2451 2683 3004 3752 3917 5363 6120 6797
Size: 845 232 321 748 165 1446 757 677
Cuts at: 6797 7945 8190 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1446 1148 845 757 748 677 321
245 232 165
NaeI GCC'GGC
Cuts at: 3731 3731
Size: 8704
NcoI C'CATG_G
Cuts at: 513 1065 2409 513
Size: 552 1344 6808
Fragments arranged by size:
6808 1344 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8704
NgoMIV G'CCGG_C
Cuts at: 3729 3729
Size: 8704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8704
NotI GC'GGCC_GC
Cuts at: 6151 6151
Size: 8704
NsiI A_TGCA'T
Cuts at: 4484 5773 4484
Size: 1289 7415
NspI r_CATG'y
Cuts at: 1132 1229 3760 4486 4507 6062 7607 1132
Size: 97 2531 726 21 1555 1545 2229
Fragments arranged by size:
2531 2229 1555 1545 726 97 21
PasI CC'CwG_GG
Cuts at: 1348 4759 1348
Size: 3411 5293
PciI A'CATG_T
Cuts at: 7603 7603
Size: 8704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3435 4908 1108
Size: 2327 1473 4904
Fragments arranged by size:
4904 2327 1473
PfoI T'CCnGG_A
Cuts at: 2994 3278 2994
Size: 284 8420
PmeI GTTT'AAAC
Cuts at: 6008 6008
Size: 8704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 6773 6805 8286 8318 2795
Size: 32 3946 32 1481 32 3181
Fragments arranged by size:
3946 3181 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2992 4159 4858 2992
Size: 1167 699 6838
Fragments arranged by size:
6838 1167 699
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8704
PsiI TTA'TAA
Cuts at: 6274 6274
Size: 8704
PspOMI G'GGCC_C
Cuts at: 3843 4754 3843
Size: 911 7793
PspXI vC'TCGA_Gb
Cuts at: 5850 5850
Size: 8704
PstI C_TGCA'G
Cuts at: 830 2190 3456 4822 6056 830
Size: 1360 1266 1366 1234 3478
Fragments arranged by size:
3478 1366 1360 1266 1234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8704
PvuII CAG'CTG
Cuts at: 2683 3004 5363 2683
Size: 321 2359 6024
Fragments arranged by size:
6024 2359 321
SacI G_AGCT'C
Cuts at: 4293 5167 5857 4293
Size: 874 690 7140
Fragments arranged by size:
7140 874 690
SalI G'TCGA_C
Cuts at: 1936 6046 1936
Size: 4110 4594
SapI GCTCTTCn'nnn_
Cuts at: 3689 3689
Size: 8704
SbfI CC_TGCA'GG
Cuts at: 6056 6056
Size: 8704
ScaI AGT'ACT
Cuts at: 6952 8664 6952
Size: 1712 6992
SfcI C'TryA_G
Cuts at: 826 1045 2186 2461 3181 3235 3452 4818 5425
Size: 219 1141 275 720 54 217 1366 607
Cuts at: 5425 6052 7187 7868 8059 826
Size: 627 1135 681 191 1471
Fragments arranged by size:
1471 1366 1141 1135 720 681 627 607
275 219 217 191 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 3110 3110
Size: 8704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8704
SmaI CCC'GGG
Cuts at: 1872 3842 6031 1872
Size: 1970 2189 4545
Fragments arranged by size:
4545 2189 1970
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 2747 3404 4583 4883 5850
Size: 197 926 786 18 657 1179 300 967
Cuts at: 5850 6808 7709 7971 820
Size: 958 901 262 1553
Fragments arranged by size:
1553 1179 967 958 926 901 786 657
300 262 197 18
SnaBI TAC'GTA
Cuts at: 493 6495 493
Size: 6002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8704
SphI G_CATG'C
Cuts at: 1132 4486 6062 1132
Size: 3354 1576 3774
Fragments arranged by size:
3774 3354 1576
SspI AAT'ATT
Cuts at: 5 52 6628 5
Size: 47 6576 2081
Fragments arranged by size:
6576 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 3275 3764 5130 1517
Size: 1203 555 489 1366 5091
Fragments arranged by size:
5091 1366 1203 555 489
StyI C'CwwG_G
Cuts at: 513 1065 2409 2434 3300 3699 4054 5125 5614
Size: 552 1344 25 866 399 355 1071 489
Cuts at: 5614 6143 513
Size: 529 3074
Fragments arranged by size:
3074 1344 1071 866 552 529 489 399
355 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4631 5428 6731 6748 6901 7086 7531
Size: 1255 3051 797 1303 17 153 185 445
Cuts at: 7531 325
Size: 1498
Fragments arranged by size:
3051 1498 1303 1255 797 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 3780 4845
Size: 274 80 33 51 1443 399 1404 1065
Cuts at: 4845 5076 6950 8662 96
Size: 231 1874 1712 138
Fragments arranged by size:
1874 1712 1443 1404 1065 399 274 231
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3593 3625 8664 8696 3512
Size: 32 49 32 5039 32 3520
Fragments arranged by size:
5039 3520 49 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5354 1316
Size: 4038 4666
XbaI T'CTAG_A
Cuts at: 6040 6040
Size: 8704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2776 2221
Size: 555 8149
XhoI C'TCGA_G
Cuts at: 1943 5850 1943
Size: 3907 4797
XmaI C'CCGG_G
Cuts at: 1870 3840 6029 1870
Size: 1970 2189 4545
Fragments arranged by size:
4545 2189 1970
XmnI GAAnn'nnTTC
Cuts at: 2429 5453 6833 2429
Size: 3024 1380 4300
Fragments arranged by size:
4300 3024 1380
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6512 276
Size: 53 83 186 936 4978 2468
Fragments arranged by size:
4978 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI PacI
PmlI PsrI RsrII SacII SanDI SexAI SfoI SrfI
SwaI