Restriction map for
pFN21AE2468
(Circular) (Six-base) MAPSORT of: pFN21AE2468.seq Check: 5625 from: 1 to: 6409
pFN21AE2468 6409 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2224 2224
Size: 6409
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3752 1937
Size: 1815 4594
Acc65I G'GTAC_C
Cuts at: 3730 3730
Size: 6409
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4472 5856 6124 1632
Size: 2840 1384 268 1917
Fragments arranged by size:
2840 1917 1384 268
AfeI AGC'GCT
Cuts at: 2309 2309
Size: 6409
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 5308 6285 1203
Size: 4105 977 1327
Fragments arranged by size:
4105 1327 977
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3254 1184
Size: 2070 4339
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 6377
AlwNI CAG_nnn'CTG
Cuts at: 2221 4152 5724 2221
Size: 1931 1572 2906
Fragments arranged by size:
2906 1931 1572
ApaI G_GGCC'C
Cuts at: 2937 2937
Size: 6409
ApaLI G'TGCA_C
Cuts at: 2152 3388 4466 5622 2152
Size: 1236 1078 1156 2939
Fragments arranged by size:
2939 1236 1156 1078
ApoI r'AATT_y
Cuts at: 1493 3718 3944 4096 6205 1493
Size: 2225 226 152 2109 1697
Fragments arranged by size:
2225 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 3000 3283 3734 1870
Size: 73 1057 283 451 4545
Fragments arranged by size:
4545 1057 451 283 73
BamHI G'GATC_C
Cuts at: 3272 3739 4106 3272
Size: 467 367 5575
Fragments arranged by size:
5575 467 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2663 3558 3730 5185
Size: 325 217 627 662 214 895 172 1455
Cuts at: 5185 618
Size: 1842
Fragments arranged by size:
1842 1455 895 662 627 325 217 214
172
BanII G_rGCy'C
Cuts at: 1391 2937 3321 3728 1391
Size: 1546 384 407 4072
Fragments arranged by size:
4072 1546 407 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 3607 928
Size: 971 702 1006 3730
Fragments arranged by size:
3730 1006 971 702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2725 2759 2876 2910 4600 4634 1903
Size: 34 788 34 117 34 1690 34 3678
Fragments arranged by size:
3678 1690 788 117 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4301 5511 4301
Size: 1210 5199
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2371 2504 3025
Size: 107 122 71 631 605 699 133 521
Cuts at: 3025 3080 5020 136
Size: 55 1940 1525
Fragments arranged by size:
1940 1525 699 631 605 521 133 122
107 71 55
BglII A'GATC_T
Cuts at: 6404 6404
Size: 6409
BlpI GC'TnA_GC
Cuts at: 2018 3826 2018
Size: 1808 4601
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2454 2666 2937 3225 3336 3392 3561
Size: 366 298 212 271 288 111 56 169
Cuts at: 3561 4470 5626 1790
Size: 909 1156 2573
Fragments arranged by size:
2573 1156 909 366 298 288 271 212
169 111 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3691 4140 5098
Size: 416 53 83 399 24 2274 449 958
Cuts at: 5098 442
Size: 1753
Fragments arranged by size:
2274 1753 958 449 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 2534 2804 5069 1239
Size: 973 322 270 2265 2579
Fragments arranged by size:
2579 2265 973 322 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3548 4534 5399 5697 2885
Size: 663 986 865 298 3597
Fragments arranged by size:
3597 986 865 663 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2997 5072 882
Size: 617 1498 2075 2219
Fragments arranged by size:
2219 2075 1498 617
BsaAI yAC'GTr
Cuts at: 493 1204 4200 493
Size: 711 2996 2702
Fragments arranged by size:
2996 2702 711
BsaBI GATnn'nnATC
Cuts at: 6403 6403
Size: 6409
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2394 4216 4598 5233
Size: 53 83 186 936 861 1822 382 635
Cuts at: 5233 275
Size: 1451
Fragments arranged by size:
1822 1451 936 861 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2922 4842 5240 5514 5661 6072 2922
Size: 1920 398 274 147 411 3259
Fragments arranged by size:
3259 1920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6184
Fragments arranged by size:
6184 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2930 1186
Size: 1150 594 4665
Fragments arranged by size:
4665 1150 594
BseYI C'CCAG_C
Cuts at: 2380 2929 5612 6108 2380
Size: 549 2683 496 2681
Fragments arranged by size:
2683 2681 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2352 1163
Size: 573 567 49 5220
Fragments arranged by size:
5220 573 567 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3859 4620 5648 1540
Size: 275 186 1858 761 1028 2301
Fragments arranged by size:
2301 1858 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 2703 3392 4470 4555 5626 2156
Size: 547 689 1078 85 1071 2939
Fragments arranged by size:
2939 1078 1071 689 547 85
BsmI GAATG_Cn'
Cuts at: 3920 4013 3920
Size: 93 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2454 2666 2703 2937 3225 3321
Size: 399 366 298 212 37 234 288 96
Cuts at: 3321 3336 3392 3561 3728 4470 4555 5626 1391
Size: 15 56 169 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 298 288 234
212 169 167 96 85 56 37 15
BspEI T'CCGG_A
Cuts at: 6072 6072
Size: 6409
BspHI T'CATG_A
Cuts at: 4298 6028 4298
Size: 1730 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 2325 3764 844
Size: 1380 101 1439 3489
Fragments arranged by size:
3489 1439 1380 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2605 4296 1394
Size: 429 245 537 1691 3507
Fragments arranged by size:
3507 1691 537 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2507 5053 5240 1700
Size: 110 697 2546 187 2869
Fragments arranged by size:
2869 2546 697 187 110
BsrGI T'GTAC_A
Cuts at: 96 3468 96
Size: 3372 3037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 2744 4469 5481 2744
Size: 1725 1012 3672
Fragments arranged by size:
3672 1725 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3331 794
Size: 2537 3872
BstBI TT'CG_AA
Cuts at: 5213 5213
Size: 6409
BstEII G'GTnAC_C
Cuts at: 1184 3205 1184
Size: 2021 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 3091 2788
Size: 303 6106
BstYI r'GATC_y
Cuts at: 1972 3272 3739 4106 4491 4508 5960 6046 6058
Size: 1300 467 367 385 17 1452 86 12
Cuts at: 6058 6404 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1300 467 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 2436 513
Size: 552 939 291 141 4486
Fragments arranged by size:
4486 939 552 291 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5670
Fragments arranged by size:
5670 683 56
BtsI GCAGTG_nn'
Cuts at: 3336 3925 4719 4739 3336
Size: 589 794 20 5006
Fragments arranged by size:
5006 794 589 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3381 3416 529
Size: 35 2817 35 3522
Fragments arranged by size:
3522 2817 35 35
DraI TTT'AAA
Cuts at: 2848 3713 2848
Size: 865 5544
DraIII CAC_nnn'GTG
Cuts at: 3331 3387 3331
Size: 56 6353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5416 809
Size: 507 4100 1802
Fragments arranged by size:
4100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2292 2439
Size: 54 1425 50 275 18 309 153 147
Cuts at: 2439 2649 3081 3093 3402 3522 3856 4745 6374
Size: 210 432 12 309 120 334 889 1629
Cuts at: 6374 8
Size: 43
Fragments arranged by size:
1629 1425 889 432 334 309 309 275
210 153 147 120 54 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 3856 1537
Size: 275 2044 4090
Fragments arranged by size:
4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2603 4339 1353
Size: 863 387 1736 3423
Fragments arranged by size:
3423 1736 863 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3267 4996 5370 5516 387
Size: 1064 138 1678 1729 374 146 1280
Fragments arranged by size:
1729 1678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2534 2804 4472 5069 5856 6124
Size: 393 580 322 270 1668 597 787 268
Cuts at: 6124 1239
Size: 1524
Fragments arranged by size:
1668 1524 787 597 580 393 322 270
268
EcoO109I rG'GnC_Cy
Cuts at: 2573 2687 2740 2933 2934 2573
Size: 114 53 193 1 6048
Fragments arranged by size:
6048 193 114 53 1
EcoRI G'AATT_C
Cuts at: 3718 4096 3718
Size: 378 6031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 6409
FseI GG_CCGG'CC
Cuts at: 2511 2511
Size: 6409
FspI TGC'GCA
Cuts at: 2339 4915 2339
Size: 2576 3833
HaeII r_GCGC'y
Cuts at: 1678 2018 2311 5556 1678
Size: 340 293 3245 2531
Fragments arranged by size:
3245 2531 340 293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5050 5082 5124 5156 1373
Size: 32 135 32 3478 32 42 32 2626
Fragments arranged by size:
3478 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3637 3753 3999 669
Size: 988 281 1699 116 246 3079
Fragments arranged by size:
3079 1699 988 281 246 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 3734 3734
Size: 6409
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5523 5707 1305
Size: 4218 184 2007
Fragments arranged by size:
4218 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 3083 3095 3404 3524
Size: 54 1425 343 309 942 12 309 120
Cuts at: 3524 10
Size: 2895
Fragments arranged by size:
2895 1425 942 343 309 309 120 54
12
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2135 2264 2441 2447 2789 3089
Size: 648 927 42 129 177 6 342 300
Cuts at: 3089 4367 4726 4885 518
Size: 1278 359 159 2042
Fragments arranged by size:
2042 1278 927 648 359 342 300 177
159 129 42 6
MspA1I CmG'CkG
Cuts at: 1606 3466 3825 4502 5650 5895 1606
Size: 1860 359 677 1148 245 2120
Fragments arranged by size:
2120 1860 1148 677 359 245
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 6409
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4918
Fragments arranged by size:
4918 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 2269 2788 3767 5312 1132
Size: 97 1040 519 979 1545 2229
Fragments arranged by size:
2229 1545 1040 979 519 97
PasI CC'CwG_GG
Cuts at: 1348 3225 1348
Size: 1877 4532
PciI A'CATG_T
Cuts at: 5308 5308
Size: 6409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2148 2221 1108
Size: 1040 73 5296
Fragments arranged by size:
5296 1040 73
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2687 2740 2687
Size: 53 6356
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 3979 3979
Size: 6409
PspOMI G'GGCC_C
Cuts at: 2933 2933
Size: 6409
PstI C_TGCA'G
Cuts at: 830 2913 3761 830
Size: 2083 848 3478
Fragments arranged by size:
3478 2083 848
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SanDI GG'GwC_CC
Cuts at: 2687 2687
Size: 6409
SbfI CC_TGCA'GG
Cuts at: 3761 3761
Size: 6409
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SfcI C'TryA_G
Cuts at: 826 1045 2909 3757 4892 5573 5764 826
Size: 219 1864 848 1135 681 191 1471
Fragments arranged by size:
1864 1471 1135 848 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 3736 1872
Size: 1864 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 3563 4513 5414 5676 820
Size: 197 926 957 663 950 901 262 1553
Fragments arranged by size:
1553 957 950 926 901 663 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SspI AAT'ATT
Cuts at: 5 52 4333 5
Size: 47 4281 2081
Fragments arranged by size:
4281 2081 47
StuI AGG'CCT
Cuts at: 1517 3281 1517
Size: 1764 4645
StyI C'CwwG_G
Cuts at: 513 1065 2004 2538 3848 513
Size: 552 939 534 1310 3074
Fragments arranged by size:
3074 1310 939 552 534
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 3031 4436 4453 4606 4791 5236
Size: 1255 761 690 1405 17 153 185 445
Cuts at: 5236 325
Size: 1498
Fragments arranged by size:
1498 1405 1255 761 690 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 3468 4655
Size: 274 80 33 51 1443 270 1221 1187
Cuts at: 4655 6367 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1221 1187 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 6369 6401 2679
Size: 32 3658 32 2687
Fragments arranged by size:
3658 2687 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6409
XbaI T'CTAG_A
Cuts at: 3745 3745
Size: 6409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 3661 2443
Size: 1218 5191
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 3734 1870
Size: 1864 4545
XmnI GAAnn'nnTTC
Cuts at: 4538 4538
Size: 6409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI