Restriction map for
pFN21AE2466
(Circular) (Six-base) MAPSORT of: pFN21AE2466.seq Check: 5686 from: 1 to: 7663
pFN21AE2466 7663 bp
With 182 enzymes: SgfI *
August 29, 2014 11:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5473 278
Size: 53 83 186 936 3937 2468
Fragments arranged by size:
3937 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5006 1937
Size: 3069 4594
Acc65I G'GTAC_C
Cuts at: 4984 4984
Size: 7663
AclI AA'CG_TT
Cuts at: 1563 5790 6163 1563
Size: 4227 373 3063
Fragments arranged by size:
4227 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 2546 3425 3863 4103 4109 5726 7110
Size: 520 394 879 438 240 6 1617 1384
Cuts at: 7110 7378 1632
Size: 268 1917
Fragments arranged by size:
1917 1617 1384 879 520 438 394 268
240 6
AfeI AGC'GCT
Cuts at: 2378 2378
Size: 7663
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7466
AflIII A'CryG_T
Cuts at: 1203 2777 3921 4461 6562 7539 1203
Size: 1574 1144 540 2101 977 1327
Fragments arranged by size:
2101 1574 1327 1144 977 540
AgeI A'CCGG_T
Cuts at: 6494 6494
Size: 7663
AhdI GACnn_n'nnGTC
Cuts at: 6392 6392
Size: 7663
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4022 1184
Size: 2838 4825
AlwNI CAG_nnn'CTG
Cuts at: 3168 4702 4834 4837 5406 6978 3168
Size: 1534 132 3 569 1572 3853
Fragments arranged by size:
3853 1572 1534 569 132 3
ApaLI G'TGCA_C
Cuts at: 5720 6876 5720
Size: 1156 6507
ApoI r'AATT_y
Cuts at: 1493 3853 4972 5198 5350 7459 1493
Size: 2360 1119 226 152 2109 1697
Fragments arranged by size:
2360 2109 1697 1119 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2556 6217 160
Size: 872 1524 3661 1606
Fragments arranged by size:
3661 1606 1524 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7663
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2679 3910 4988 1870
Size: 73 281 455 1231 1078 4545
Fragments arranged by size:
4545 1231 1078 455 281 73
BamHI G'GATC_C
Cuts at: 4993 5360 4993
Size: 367 7296
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4984 6439 618
Size: 325 217 627 3197 1455 1842
Fragments arranged by size:
3197 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 2349 3182 4103 4167 4320 4982 1391
Size: 890 68 833 921 64 153 662 4072
Fragments arranged by size:
4072 921 890 833 662 153 68 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3052 3226 3238 4684 928
Size: 971 1153 174 12 1446 3907
Fragments arranged by size:
3907 1446 1153 971 174 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5854 5888 1903
Size: 34 3917 34 3678
Fragments arranged by size:
3917 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5555 6765 5555
Size: 1210 6453
BclI T'GATC_A
Cuts at: 1548 4200 1548
Size: 2652 5011
BfrBI ATG'CAT
Cuts at: 3987 3987
Size: 7663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3260 3647 4664
Size: 107 122 71 631 605 1588 387 1017
Cuts at: 4664 4676 6274 136
Size: 12 1598 1525
Fragments arranged by size:
1598 1588 1525 1017 631 605 387 122
107 71 12
BglII A'GATC_T
Cuts at: 2760 4584 7658 2760
Size: 1824 3074 2765
Fragments arranged by size:
3074 2765 1824
BlpI GC'TnA_GC
Cuts at: 2634 3181 3995 5080 2634
Size: 547 814 1085 5217
Fragments arranged by size:
5217 1085 814 547
Bme1580I G_kGCm'C
Cuts at: 1790 2111 2923 3143 5724 6880 1790
Size: 321 812 220 2581 1156 2573
Fragments arranged by size:
2581 2573 1156 812 321 220
BmgBI CAC'GTC
Cuts at: 1318 2778 4428 1318
Size: 1460 1650 4553
Fragments arranged by size:
4553 1650 1460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4741 5394 6352
Size: 416 53 83 399 24 3324 653 958
Cuts at: 6352 442
Size: 1753
Fragments arranged by size:
3324 1753 958 653 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7663
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 7663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2444 2450 2582 4181 6323 1239
Size: 1193 12 6 132 1599 2142 2579
Fragments arranged by size:
2579 2142 1599 1193 132 12 6
Bpu10I CC'TnA_GC
Cuts at: 2338 2338
Size: 7663
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5788 6653 6951 5788
Size: 865 298 6500
Fragments arranged by size:
6500 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 3571 6326 882
Size: 617 1056 1016 2755 2219
Fragments arranged by size:
2755 2219 1056 1016 617
BsaAI yAC'GTr
Cuts at: 493 1204 3877 3922 5454 493
Size: 711 2673 45 1532 2702
Fragments arranged by size:
2702 2673 1532 711 45
BsaBI GATnn'nnATC
Cuts at: 3029 4049 7657 3029
Size: 1020 3608 3035
Fragments arranged by size:
3608 3035 1020
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2321 3612 3907 4296
Size: 53 83 186 936 788 1291 295 389
Cuts at: 4296 4956 5470 5852 6487 275
Size: 660 514 382 635 1451
Fragments arranged by size:
1451 1291 936 788 660 635 514 389
382 295 186 83 53
BsaWI w'CCGG_w
Cuts at: 2232 6096 6494 6768 6915 7326 2232
Size: 3864 398 274 147 411 2569
Fragments arranged by size:
3864 2569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3551 3581 3614 3644 4493
Size: 30 165 30 2148 30 33 30 849
Cuts at: 4493 4523 4625 4655 1178
Size: 30 102 30 4186
Fragments arranged by size:
4186 2148 849 165 102 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 3359 3464 3649 3827 4130 4501 4829
Size: 1219 954 105 185 178 303 371 328
Cuts at: 4829 1186
Size: 4020
Fragments arranged by size:
4020 1219 954 371 328 303 185 178
105
BseYI C'CCAG_C
Cuts at: 3007 3098 3315 3544 4009 4927 4934 6866 7362
Size: 91 217 229 465 918 7 1932 496
Cuts at: 7362 3007
Size: 3308
Fragments arranged by size:
3308 1932 918 496 465 229 217 91
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 2521 2837 4601 1163
Size: 573 537 248 316 1764 4225
Fragments arranged by size:
4225 1764 573 537 316 248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2179 5113 5874 6902 1540
Size: 275 186 39 139 2934 761 1028 2301
Fragments arranged by size:
2934 2301 1028 761 275 186 139 39
BsiHKAI G_wGCw'C
Cuts at: 2598 2640 2747 3182 3282 4320 4791 5724 5809
Size: 42 107 435 100 1038 471 933 85
Cuts at: 5809 6880 2598
Size: 1071 3381
Fragments arranged by size:
3381 1071 1038 933 471 435 107 100
85 42
BsmI GAATG_Cn'
Cuts at: 5174 5267 5174
Size: 93 7570
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2281 2349 2598 2640 2747 2923
Size: 399 321 170 68 249 42 107 176
Cuts at: 2923 3143 3182 3282 4103 4167 4320 4791 4982
Size: 220 39 100 821 64 153 471 191
Cuts at: 4982 5724 5809 6880 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 821 742 471 399 321 249
220 191 176 170 153 107 100 85
68 64 42 39
BspEI T'CCGG_A
Cuts at: 7326 7326
Size: 7663
BspHI T'CATG_A
Cuts at: 3579 4713 5552 7282 3579
Size: 1134 839 1730 3960
Fragments arranged by size:
3960 1730 1134 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 3167 4581 5018 844
Size: 1259 1064 1414 437 3489
Fragments arranged by size:
3489 1414 1259 1064 437
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 4861 5550 1394
Size: 429 611 2427 689 3507
Fragments arranged by size:
3507 2427 689 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6158 6332 65
Size: 1183 4910 174 1396
Fragments arranged by size:
4910 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 3435 3894 4327 6307 6494 1700
Size: 110 368 1257 459 433 1980 187 2869
Fragments arranged by size:
2869 1980 1257 459 433 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 2780 3015 3924 4464 96
Size: 2684 235 909 540 3295
Fragments arranged by size:
3295 2684 909 540 235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7663
BssSI C'ACGA_G
Cuts at: 2363 2809 4112 5723 6735 2363
Size: 446 1303 1611 1012 3291
Fragments arranged by size:
3291 1611 1303 1012 446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4834 794
Size: 4040 3623
BstBI TT'CG_AA
Cuts at: 6467 6467
Size: 7663
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7663
BstXI CCAn_nnnn'nTGG
Cuts at: 2959 3274 3391 2959
Size: 315 117 7231
Fragments arranged by size:
7231 315 117
BstYI r'GATC_y
Cuts at: 1972 2611 2760 4284 4584 4770 4993 5360 5745
Size: 639 149 1524 300 186 223 367 385
Cuts at: 5745 5762 7214 7300 7312 7658 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1524 1452 639 385 367 346 300
223 186 149 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2455 2566 3081 3192 3228 3489 3633
Size: 552 1390 111 515 111 36 261 144
Cuts at: 3633 4616 4948 513
Size: 983 332 3228
Fragments arranged by size:
3228 1390 983 552 515 332 261 144
111 111 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6924
Fragments arranged by size:
6924 683 56
BtsI GCAGTG_nn'
Cuts at: 2849 3594 5179 5973 5993 2849
Size: 745 1585 794 20 4519
Fragments arranged by size:
4519 1585 794 745 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7663
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7628
DraI TTT'AAA
Cuts at: 4967 4967
Size: 7663
DraIII CAC_nnn'GTG
Cuts at: 3138 3138
Size: 7663
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2051 6670 809
Size: 507 735 4619 1802
Fragments arranged by size:
4619 1802 735 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2176 2820
Size: 54 1425 50 275 18 207 139 644
Cuts at: 2820 3636 3648 5110 5999 7628 8
Size: 816 12 1462 889 1629 43
Fragments arranged by size:
1629 1462 1425 889 816 644 275 207
139 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2176 5110 1537
Size: 275 225 139 2934 4090
Fragments arranged by size:
4090 2934 275 225 139
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 2762 2907 3218 3330 3336 3945 4121
Size: 1298 111 145 311 112 6 609 176
Cuts at: 4121 5593 1353
Size: 1472 3423
Fragments arranged by size:
3423 1472 1298 609 311 176 145 112
111 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4277 6250 6624 6770 387
Size: 1064 138 2688 1973 374 146 1280
Fragments arranged by size:
2688 1973 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3180 4318 3180
Size: 1138 6525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2432 2444 2450 2546 2582 3425
Size: 393 520 280 12 6 96 36 843
Cuts at: 3425 3863 4103 4109 4181 5726 6323 7110 7378
Size: 438 240 6 72 1545 597 787 268
Cuts at: 7378 1239
Size: 1524
Fragments arranged by size:
1545 1524 843 787 597 520 438 393
280 268 240 96 72 36 12 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 3743 2411
Size: 1332 6331
EcoO109I rG'GnC_Cy
Cuts at: 2220 2643 3319 3787 2220
Size: 423 676 468 6096
Fragments arranged by size:
6096 676 468 423
EcoRI G'AATT_C
Cuts at: 3853 4972 5350 3853
Size: 1119 378 6166
Fragments arranged by size:
6166 1119 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 7663
FspI TGC'GCA
Cuts at: 6169 6169
Size: 7663
HaeII r_GCGC'y
Cuts at: 1678 2380 3436 6810 1678
Size: 702 1056 3374 2531
Fragments arranged by size:
3374 2531 1056 702
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2321 2353 3114
Size: 32 135 32 683 32 34 32 761
Cuts at: 3114 3146 6304 6336 6378 6410 1373
Size: 32 3158 32 42 32 2626
Fragments arranged by size:
3158 2626 761 683 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2014 3456 4498 5007 5253 669
Size: 988 281 76 1442 1042 509 246 3079
Fragments arranged by size:
3079 1442 1042 988 509 281 246 76
HindIII A'AGCT_T
Cuts at: 748 3805 4388 748
Size: 3057 583 4023
Fragments arranged by size:
4023 3057 583
HpaI GTT'AAC
Cuts at: 5253 5253
Size: 7663
KpnI G_GTAC'C
Cuts at: 4988 4988
Size: 7663
MfeI C'AATT_G
Cuts at: 5262 5262
Size: 7663
MluI A'CGCG_T
Cuts at: 7539 7539
Size: 7663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3702 3883 4602 6777 6961 1305
Size: 2397 181 719 2175 184 2007
Fragments arranged by size:
2397 2175 2007 719 184 181
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 3650 10
Size: 54 1425 343 990 828 4023
Fragments arranged by size:
4023 1425 990 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2367 2556 3023 3107 3272 3578
Size: 648 1035 166 189 467 84 165 306
Cuts at: 3578 4004 4196 4712 5621 5980 6139 518
Size: 426 192 516 909 359 159 2042
Fragments arranged by size:
2042 1035 909 648 516 467 426 359
306 192 189 166 165 159 84
MspA1I CmG'CkG
Cuts at: 1606 3315 4021 4138 4834 5079 5756 6904 7149
Size: 1709 706 117 696 245 677 1148 245
Cuts at: 7149 1606
Size: 2120
Fragments arranged by size:
2120 1709 1148 706 696 677 245 245
117
NaeI GCC'GGC
Cuts at: 2180 3437 2180
Size: 1257 6406
NcoI C'CATG_G
Cuts at: 513 1065 3081 3489 3633 513
Size: 552 2016 408 144 4543
Fragments arranged by size:
4543 2016 552 408 144
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7663
NgoMIV G'CCGG_C
Cuts at: 2178 3435 2178
Size: 1257 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7663
NotI GC'GGCC_GC
Cuts at: 5110 5110
Size: 7663
NsiI A_TGCA'T
Cuts at: 3989 3989
Size: 7663
NspI r_CATG'y
Cuts at: 1132 1229 4465 5021 6566 1132
Size: 97 3236 556 1545 2229
Fragments arranged by size:
3236 2229 1545 556 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7663
PciI A'CATG_T
Cuts at: 4461 6562 4461
Size: 2101 5562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4702 1108
Size: 3594 4069
PfoI T'CCnGG_A
Cuts at: 2409 3738 4740 2409
Size: 1329 1002 5332
Fragments arranged by size:
5332 1329 1002
PmeI GTTT'AAAC
Cuts at: 4967 4967
Size: 7663
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5732 5764 7245 7277 5732
Size: 32 1481 32 6118
Fragments arranged by size:
6118 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3319 3787 3319
Size: 468 7195
PshAI GACnn'nnGTC
Cuts at: 1613 2240 1613
Size: 627 7036
PsiI TTA'TAA
Cuts at: 5233 5233
Size: 7663
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2764 2796 2764
Size: 32 7631
PstI C_TGCA'G
Cuts at: 830 3164 4592 5015 830
Size: 2334 1428 423 3478
Fragments arranged by size:
3478 2334 1428 423
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7663
PvuII CAG'CTG
Cuts at: 4021 4834 4021
Size: 813 6850
SacI G_AGCT'C
Cuts at: 3182 4320 3182
Size: 1138 6525
SalI G'TCGA_C
Cuts at: 1936 5005 1936
Size: 3069 4594
SbfI CC_TGCA'GG
Cuts at: 5015 5015
Size: 7663
ScaI AGT'ACT
Cuts at: 2024 5911 7623 2024
Size: 3887 1712 2064
Fragments arranged by size:
3887 2064 1712
SexAI A'CCwGG_T
Cuts at: 2958 4701 2958
Size: 1743 5920
SfcI C'TryA_G
Cuts at: 826 1045 3160 3622 4588 5011 6146 6827 7018
Size: 219 2115 462 966 423 1135 681 191
Cuts at: 7018 826
Size: 1471
Fragments arranged by size:
2115 1471 1135 966 681 462 423 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7663
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7663
SmaI CCC'GGG
Cuts at: 1872 2681 3912 4990 1872
Size: 809 1231 1078 4545
Fragments arranged by size:
4545 1231 1078 809
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5767 6668 6930 820
Size: 197 926 3824 901 262 1553
Fragments arranged by size:
3824 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5454 493
Size: 4961 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7663
SphI G_CATG'C
Cuts at: 1132 5021 1132
Size: 3889 3774
SrfI GCCC'GGGC
Cuts at: 3912 3912
Size: 7663
SspI AAT'ATT
Cuts at: 5 52 5587 5
Size: 47 5535 2081
Fragments arranged by size:
5535 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7663
StyI C'CwwG_G
Cuts at: 513 1065 3081 3489 3633 3645 4644 4752 5102
Size: 552 2016 408 144 12 999 108 350
Cuts at: 5102 513
Size: 3074
Fragments arranged by size:
3074 2016 999 552 408 350 144 108
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 5690 5707 5860 6045 6490 325
Size: 1255 1455 2655 17 153 185 445 1498
Fragments arranged by size:
2655 1498 1455 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2163 2780
Size: 274 80 33 51 1443 45 141 617
Cuts at: 2780 2793 2994 3015 3924 4188 4464 5909 7621
Size: 13 201 21 909 264 276 1445 1712
Cuts at: 7621 96
Size: 138
Fragments arranged by size:
1712 1445 1443 909 617 276 274 264
201 141 138 80 51 45 33 21
13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4493 4525 7623 7655 4493
Size: 32 3098 32 4501
Fragments arranged by size:
4501 3098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2960 4733 1316
Size: 1644 1773 4246
Fragments arranged by size:
4246 1773 1644
XbaI T'CTAG_A
Cuts at: 4999 4999
Size: 7663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 3874 4558 2797
Size: 1077 684 5902
Fragments arranged by size:
5902 1077 684
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7663
XmaI C'CCGG_G
Cuts at: 1870 2679 3910 4988 1870
Size: 809 1231 1078 4545
Fragments arranged by size:
4545 1231 1078 809
XmnI GAAnn'nnTTC
Cuts at: 5792 5792
Size: 7663
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5471 276
Size: 53 83 186 936 3937 2468
Fragments arranged by size:
3937 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI RsrII SacII
SanDI SapI SfiI SfoI SwaI