Restriction map for
pFN21AE2461
(Circular) (Six-base) MAPSORT of: pFN21AE2461.seq Check: 302 from: 1 to: 6466
pFN21AE2461 6466 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2149
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3588 3809 1937
Size: 1651 221 4594
Fragments arranged by size:
4594 1651 221
Acc65I G'GTAC_C
Cuts at: 2301 3238 3787 2301
Size: 937 549 4980
Fragments arranged by size:
4980 937 549
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3517 4529 5913 6181 1632
Size: 1885 1012 1384 268 1917
Fragments arranged by size:
1917 1885 1384 1012 268
AfeI AGC'GCT
Cuts at: 3374 3374
Size: 6466
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 5365 6342 1203
Size: 4162 977 1327
Fragments arranged by size:
4162 1327 977
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 2912 3026 1166
Size: 1746 114 4606
Fragments arranged by size:
4606 1746 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3620 1184
Size: 2436 4030
AlwNI CAG_nnn'CTG
Cuts at: 2742 4209 5781 2742
Size: 1467 1572 3427
Fragments arranged by size:
3427 1572 1467
ApaLI G'TGCA_C
Cuts at: 3400 4523 5679 3400
Size: 1123 1156 4187
Fragments arranged by size:
4187 1156 1123
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 3791 1870
Size: 73 231 1617 4545
Fragments arranged by size:
4545 1617 231 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3222 3255 3222
Size: 33 6433
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 2494 2716 2754 2875
Size: 325 217 627 514 193 222 38 121
Cuts at: 2875 3012 3238 3787 5242 618
Size: 137 226 549 1455 1842
Fragments arranged by size:
1842 1455 627 549 514 325 226 222
217 193 137 121 38
BanII G_rGCy'C
Cuts at: 1391 2391 2486 3074 3785 1391
Size: 1000 95 588 711 4072
Fragments arranged by size:
4072 1000 711 588 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 928
Size: 971 180 5315
Fragments arranged by size:
5315 971 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3634 3668 4657 4691 1903
Size: 34 50 34 1613 34 989 34 3678
Fragments arranged by size:
3678 1613 989 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 3294 1548
Size: 1746 4720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2031 3102 5077
Size: 107 122 71 631 605 359 1071 1975
Cuts at: 5077 136
Size: 1525
Fragments arranged by size:
1975 1525 1071 631 605 359 122 107
71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 2480 3301 3632 3883 2480
Size: 821 331 251 5063
Fragments arranged by size:
5063 821 331 251
Bme1580I G_kGCm'C
Cuts at: 1790 2499 2757 2805 2878 3404 4527 5683 1790
Size: 709 258 48 73 526 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 709 526 258 73 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4197 5155 442
Size: 416 53 83 399 24 2780 958 1753
Fragments arranged by size:
2780 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2420 2594 2956 3430 2135
Size: 285 174 362 474 5171
Fragments arranged by size:
5171 474 362 285 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2643 5126 1239
Size: 1404 2483 2579
Fragments arranged by size:
2579 2483 1404
Bpu10I CC'TnA_GC
Cuts at: 3641 3641
Size: 6466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3416 4591 5456 5754 3416
Size: 1175 865 298 4128
Fragments arranged by size:
4128 1175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2391 2661 2675 3401 5129 882
Size: 617 892 270 14 726 1728 2219
Fragments arranged by size:
2219 1728 892 726 617 270 14
BsaAI yAC'GTr
Cuts at: 493 1204 3682 4257 493
Size: 711 2478 575 2702
Fragments arranged by size:
2702 2478 711 575
BsaBI GATnn'nnATC
Cuts at: 6460 6460
Size: 6466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4273 4655 5290 275
Size: 53 83 186 936 2740 382 635 1451
Fragments arranged by size:
2740 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4899 5297 5571 5718 6129 4899
Size: 398 274 147 411 5236
Fragments arranged by size:
5236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2641 2671 1178
Size: 30 165 30 1238 30 4973
Fragments arranged by size:
4973 1238 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 3443 1186
Size: 1084 1173 4209
Fragments arranged by size:
4209 1173 1084
BseYI C'CCAG_C
Cuts at: 2058 2813 3067 3309 3626 5669 6165 2058
Size: 755 254 242 317 2043 496 2359
Fragments arranged by size:
2359 2043 755 496 317 254 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1999 1163
Size: 573 263 5630
Fragments arranged by size:
5630 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4677 5705 1540
Size: 275 186 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2391 2937 3404 3720 4527 4612 5683 2361
Size: 30 546 467 316 807 85 1071 3144
Fragments arranged by size:
3144 1071 807 546 467 316 85 30
BsmI GAATG_Cn'
Cuts at: 3977 4070 3977
Size: 93 6373
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2391 2486 2499 2757 2805 2878
Size: 399 571 30 95 13 258 48 73
Cuts at: 2878 2937 3074 3404 3720 3785 4527 4612 5683
Size: 59 137 330 316 65 742 85 1071
Cuts at: 5683 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 571 399 330 316 258
137 95 85 73 65 59 48 30
13
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 2903 3074 3612 3821 844
Size: 1305 754 171 538 209 3489
Fragments arranged by size:
3489 1305 754 538 209 171
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 1394
Size: 429 2530 3507
Fragments arranged by size:
3507 2530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4961 5135 65
Size: 1183 3713 174 1396
Fragments arranged by size:
3713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5110 5297 1700
Size: 110 3300 187 2869
Fragments arranged by size:
3300 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 3745 4526 5538 3745
Size: 781 1012 4673
Fragments arranged by size:
4673 1012 781
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6466
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 2179 1184
Size: 995 5471
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2858 3278 3418 3622 2506
Size: 352 420 140 204 5350
Fragments arranged by size:
5350 420 352 204 140
BstYI r'GATC_y
Cuts at: 1972 2514 2572 3796 4163 4548 4565 6017 6103
Size: 542 58 1224 367 385 17 1452 86
Cuts at: 6103 6115 6461 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1224 542 385 367 346 86
58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2880 2880
Size: 6466
BtgI C'CryG_G
Cuts at: 513 1065 2271 3271 3576 513
Size: 552 1206 1000 305 3403
Fragments arranged by size:
3403 1206 1000 552 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5727
Fragments arranged by size:
5727 683 56
BtsI GCAGTG_nn'
Cuts at: 2436 2782 3350 3982 4776 4796 2436
Size: 346 568 632 794 20 4106
Fragments arranged by size:
4106 794 632 568 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DraIII CAC_nnn'GTG
Cuts at: 2524 2524
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5473 809
Size: 507 4157 1802
Fragments arranged by size:
4157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3579 3612 3913
Size: 54 1425 50 275 18 1749 33 301
Cuts at: 3913 4802 6431 8
Size: 889 1629 43
Fragments arranged by size:
1749 1629 1425 889 301 275 54 50
43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3913 1537
Size: 275 2101 4090
Fragments arranged by size:
4090 2101 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 2862 3548 4396 1353
Size: 881 628 686 848 3423
Fragments arranged by size:
3423 881 848 686 628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5053 5427 5573 387
Size: 1064 138 3464 374 146 1280
Fragments arranged by size:
3464 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2389 2389
Size: 6466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2643 3517 4529 5126 5913 6181 1239
Size: 393 1011 874 1012 597 787 268 1524
Fragments arranged by size:
1524 1012 1011 874 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 3488 2231
Size: 1257 5209
EcoO109I rG'GnC_Cy
Cuts at: 2817 3148 2817
Size: 331 6135
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2417 2644 2417
Size: 227 6239
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 3376 3754 5613 1678
Size: 1698 378 1859 2531
Fragments arranged by size:
2531 1859 1698 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5107 5139 5181 5213 1373
Size: 32 135 32 3535 32 42 32 2626
Fragments arranged by size:
3535 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3810 4056 669
Size: 988 281 1872 246 3079
Fragments arranged by size:
3079 1872 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KpnI G_GTAC'C
Cuts at: 2305 3242 3791 2305
Size: 937 549 4980
Fragments arranged by size:
4980 937 549
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3229 5580 5764 1305
Size: 1924 2351 184 2007
Fragments arranged by size:
2351 2007 1924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4644
Fragments arranged by size:
4644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2912 3026 4424 4783 4942 518
Size: 648 1746 114 1398 359 159 2042
Fragments arranged by size:
2042 1746 1398 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2308 3305 3313 3648 3882 4559 5707 5952
Size: 702 997 8 335 234 677 1148 245
Cuts at: 5952 1606
Size: 2120
Fragments arranged by size:
2120 1148 997 702 677 335 245 234
8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5914
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 3824 5369 1132
Size: 97 2595 1545 2229
Fragments arranged by size:
2595 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2296 3418 1348
Size: 948 1122 4396
Fragments arranged by size:
4396 1122 948
PciI A'CATG_T
Cuts at: 5365 5365
Size: 6466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 1108
Size: 1656 4810
PfoI T'CCnGG_A
Cuts at: 3557 3557
Size: 6466
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2817
Size: 6466
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PstI C_TGCA'G
Cuts at: 830 2040 2160 3479 3818 830
Size: 1210 120 1319 339 3478
Fragments arranged by size:
3478 1319 1210 339 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2308 3313 2308
Size: 1005 5461
SacI G_AGCT'C
Cuts at: 2391 2391
Size: 6466
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SapI GCTCTTCn'nnn_
Cuts at: 3548 3548
Size: 6466
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SexAI A'CCwGG_T
Cuts at: 2712 2712
Size: 6466
SfcI C'TryA_G
Cuts at: 826 1045 2036 2156 2842 3475 3814 4949 5630
Size: 219 991 120 686 633 339 1135 681
Cuts at: 5630 5821 826
Size: 191 1471
Fragments arranged by size:
1471 1135 991 686 681 633 339 219
191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 3793 1872
Size: 1921 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 4570 5471 5733 820
Size: 197 926 1452 1175 901 262 1553
Fragments arranged by size:
1553 1452 1175 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2130 3127 3728 1517
Size: 613 997 601 4255
Fragments arranged by size:
4255 997 613 601
StyI C'CwwG_G
Cuts at: 513 1065 2392 3160 3234 3905 513
Size: 552 1327 768 74 671 3074
Fragments arranged by size:
3074 1327 768 671 552 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2935 4493 4510 4663 4848 5293 325
Size: 1255 1355 1558 17 153 185 445 1498
Fragments arranged by size:
1558 1498 1355 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4712 6424 96
Size: 274 80 33 51 1443 2735 1712 138
Fragments arranged by size:
2735 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2641 2673 6426 6458 2641
Size: 32 3753 32 2649
Fragments arranged by size:
3753 2649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2293
Size: 6466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 3791 1870
Size: 1921 4545
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI