Restriction map for
pFN21AE2459
(Circular) (Six-base) MAPSORT of: pFN21AE2459.seq Check: 4913 from: 1 to: 6220
pFN21AE2459 6220 bp
With 182 enzymes: SgfI *
February 8, 2013 19:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2607 3192 4283 5667 5935 1632
Size: 779 196 585 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 779 585 268 196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2135 5119 6096 1203
Size: 932 2984 977 1327
Fragments arranged by size:
2984 1327 977 932
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 3360 3963 5535 3360
Size: 603 1572 4045
Fragments arranged by size:
4045 1572 603
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2151 2184 2466 2499 2151
Size: 33 282 33 5872
Fragments arranged by size:
5872 282 33 33
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4996 618
Size: 325 217 627 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3539 1391
Size: 2148 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4411 4445 1903
Size: 34 50 34 2390 34 3678
Fragments arranged by size:
3678 2390 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 2212 2872 1548
Size: 664 660 4896
Fragments arranged by size:
4896 664 660
BfrBI ATG'CAT
Cuts at: 2707 2707
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2982 4831 136
Size: 107 122 71 631 605 1310 1849 1525
Fragments arranged by size:
1849 1525 1310 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3239 6215 3239
Size: 2976 3244
BlpI GC'TnA_GC
Cuts at: 2535 3637 2535
Size: 1102 5118
Bme1580I G_kGCm'C
Cuts at: 1790 4281 5437 1790
Size: 2491 1156 2573
Fragments arranged by size:
2573 2491 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3261 3261
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 4880 1239
Size: 894 2747 2579
Fragments arranged by size:
2747 2579 894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2136 4011 493
Size: 711 932 1875 2702
Fragments arranged by size:
2702 1875 932 711
BsaBI GATnn'nnATC
Cuts at: 2871 3097 6214 2871
Size: 226 3117 2877
Fragments arranged by size:
3117 2877 226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2035 4027 4409 5044
Size: 53 83 186 936 502 1992 382 635
Cuts at: 5044 275
Size: 1451
Fragments arranged by size:
1992 1451 936 635 502 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2754 2784 1178
Size: 30 165 30 1351 30 4614
Fragments arranged by size:
4614 1351 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 1186
Size: 1228 4992
BseYI C'CCAG_C
Cuts at: 3116 5423 5919 3116
Size: 2307 496 3417
Fragments arranged by size:
3417 2307 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5647
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3453 4281 4366 5437 3453
Size: 828 85 1071 4236
Fragments arranged by size:
4236 1071 828 85
BsmI GAATG_Cn'
Cuts at: 3469 3731 3824 3469
Size: 262 93 5865
Fragments arranged by size:
5865 262 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3453 3539 4281 4366 5437 1391
Size: 399 1663 86 742 85 1071 2174
Fragments arranged by size:
2174 1663 1071 742 399 86 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 3445 3478 4109 5839 3445
Size: 33 631 1730 3826
Fragments arranged by size:
3826 1730 631 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 844
Size: 2731 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 3212 3212
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 3239 3550 3917 4302 4319 5771 5857 5869
Size: 1267 311 367 385 17 1452 86 12
Cuts at: 5869 6215 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1267 385 367 346 311 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2487 3736 4530 4550 2487
Size: 1249 794 20 4157
Fragments arranged by size:
4157 1249 794 20
ClaI AT'CG_AT
Cuts at: 1554 3007 1554
Size: 1453 4767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 3457 3457
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2807 3496 3667
Size: 54 1425 50 275 18 977 689 171
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 977 889 689 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2583 4150 1353
Size: 1230 1567 3423
Fragments arranged by size:
3423 1567 1230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2963 4807 5181 5327 387
Size: 1064 138 1374 1844 374 146 1280
Fragments arranged by size:
1844 1374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2411 2607 3192 4283 4880 5667
Size: 393 501 278 196 585 1091 597 787
Cuts at: 5667 5935 1239
Size: 268 1524
Fragments arranged by size:
1524 1091 787 597 585 501 393 278
268 196
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2984 3396 2984
Size: 412 5808
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 5367 1678
Size: 3689 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2973 2989 3005
Size: 32 135 32 536 32 833 16 16
Cuts at: 3005 3021 3283 3315 4861 4893 4935 4967 1373
Size: 16 262 32 1546 32 42 32 2626
Fragments arranged by size:
2626 1546 833 536 262 135 42 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3368 3564 3810 669
Size: 988 281 1430 196 246 3079
Fragments arranged by size:
3079 1430 988 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 2486 3819 2486
Size: 1333 4887
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 5334 5518 1305
Size: 972 3057 184 2007
Fragments arranged by size:
3057 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3498 10
Size: 54 1425 343 1666 2732
Fragments arranged by size:
2732 1666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3192 4178 4537 4696 518
Size: 648 2026 986 359 159 2042
Fragments arranged by size:
2042 2026 986 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2111 3636 4313 5461 5706 1606
Size: 505 1525 677 1148 245 2120
Fragments arranged by size:
2120 1525 1148 677 505 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NsiI A_TGCA'T
Cuts at: 2709 2709
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2057 2711 2931 3159 3578 5123 1132
Size: 97 828 654 220 228 419 1545 2229
Fragments arranged by size:
2229 1545 828 654 419 228 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6220
PfoI T'CCnGG_A
Cuts at: 3313 3313
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PmlI CAC'GTG
Cuts at: 2136 2136
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3363 1613
Size: 1750 4470
PsiI TTA'TAA
Cuts at: 2499 3790 2499
Size: 1291 4929
PstI C_TGCA'G
Cuts at: 830 2110 3572 830
Size: 1280 1462 3478
Fragments arranged by size:
3478 1462 1280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2111 2111
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2106 2737 3568 4703 5384 5575 826
Size: 219 1061 631 831 1135 681 191 1471
Fragments arranged by size:
1471 1135 1061 831 681 631 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 5225 5487 820
Size: 197 926 2381 901 262 1553
Fragments arranged by size:
2381 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 2931 3578 1132
Size: 1799 647 3774
Fragments arranged by size:
3774 1799 647
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2850 3659 513
Size: 552 1785 809 3074
Fragments arranged by size:
3074 1785 809 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 4247 4264 4417 4602 5047 325
Size: 1255 448 2219 17 153 185 445 1498
Fragments arranged by size:
2219 1498 1255 448 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2086 2118 6180 6212 2086
Size: 32 4062 32 2094
Fragments arranged by size:
4062 2094 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3131 3556 3131
Size: 425 5795
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI