Restriction map for
pFN21AE2451
(Circular) (Six-base) MAPSORT of: pFN21AE2451.seq Check: 1100 from: 1 to: 5839
pFN21AE2451 5839 bp
With 182 enzymes: SgfI *
February 5, 2013 11:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 3182 1937
Size: 792 453 4594
Fragments arranged by size:
4594 792 453
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 2246 3902 5286 5554 1632
Size: 426 188 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 426 268 188
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2589 2799 4738 5715 1203
Size: 1386 210 1939 977 1327
Fragments arranged by size:
1939 1386 1327 977 210
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2366 2608 1184
Size: 1182 242 4415
Fragments arranged by size:
4415 1182 242
AlwNI CAG_nnn'CTG
Cuts at: 2081 2693 2867 3582 5154 2081
Size: 612 174 715 1572 2766
Fragments arranged by size:
2766 1572 715 612 174
ApaI G_GGCC'C
Cuts at: 2670 2670
Size: 5839
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 3148 3374 3526 5635 1493
Size: 1655 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 2275 2648 3164 1870
Size: 73 332 373 516 4545
Fragments arranged by size:
4545 516 373 332 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2847 2880 2847
Size: 33 5806
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2670 3158 1391
Size: 1279 488 4072
Fragments arranged by size:
4072 1279 488
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2566 2630 2823 3120 3126 928
Size: 971 667 64 193 297 6 3641
Fragments arranged by size:
3641 971 667 297 193 64 6
BbvCI CC'TCA_GC
Cuts at: 2838 2838
Size: 5839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 2955 1548
Size: 1407 4432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2183 2670 3900 5056 1790
Size: 393 487 1230 1156 2573
Fragments arranged by size:
2573 1230 1156 487 393
BmgBI CAC'GTC
Cuts at: 1318 3097 1318
Size: 1779 4060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2304 2697 3570
Size: 416 53 83 399 24 887 393 873
Cuts at: 3570 4528 442
Size: 958 1753
Fragments arranged by size:
1753 958 887 873 416 399 393 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3038
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2734 2865 4499 1239
Size: 1298 197 131 1634 2579
Fragments arranged by size:
2579 1634 1298 197 131
Bpu10I CC'TnA_GC
Cuts at: 2838 2868 2838
Size: 30 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 3964 4829 5127 2221
Size: 1743 865 298 2933
Fragments arranged by size:
2933 1743 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 4502 882
Size: 617 646 2357 2219
Fragments arranged by size:
2357 2219 646 617
BsaAI yAC'GTr
Cuts at: 493 1204 2592 2800 3630 493
Size: 711 1388 208 830 2702
Fragments arranged by size:
2702 1388 830 711 208
BsaBI GATnn'nnATC
Cuts at: 2957 5833 2957
Size: 2876 2963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2189 2540 2620 4272 4670 4944 5091 5502 2189
Size: 351 80 1652 398 274 147 411 2526
Fragments arranged by size:
2526 1652 411 398 351 274 147 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2826 1186
Size: 1640 4199
BseYI C'CCAG_C
Cuts at: 2163 2494 5042 5538 2163
Size: 331 2548 496 2464
Fragments arranged by size:
2548 2464 496 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2484 3119 1163
Size: 573 748 635 3883
Fragments arranged by size:
3883 748 635 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2985 3900 3985 5056 2985
Size: 915 85 1071 3768
Fragments arranged by size:
3768 1071 915 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2670 2985 3158 3900 3985 5056
Size: 399 393 487 315 173 742 85 1071
Cuts at: 5056 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 487 399 393 315 173
85
BspEI T'CCGG_A
Cuts at: 2620 5502 2620
Size: 2882 2957
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 4334 4508 65
Size: 1183 1317 1769 174 1396
Fragments arranged by size:
1769 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 2102 2230 1184
Size: 918 128 4793
Fragments arranged by size:
4793 918 128
BstYI r'GATC_y
Cuts at: 1972 2779 3169 3536 3921 3938 5390 5476 5488
Size: 807 390 367 385 17 1452 86 12
Cuts at: 5488 5834 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 807 390 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2944 487
Size: 683 56 1718 3382
Fragments arranged by size:
3382 1718 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 3355 4149 4169 2676
Size: 679 794 20 4346
Fragments arranged by size:
4346 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DraIII CAC_nnn'GTG
Cuts at: 2110 2110
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804
Size: 54 1425 50 275 18 1456 889 1629
Cuts at: 5804 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2990 3769 1353
Size: 1637 779 3423
Fragments arranged by size:
3423 1637 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2246 2537 2734 2865 3902 4499
Size: 393 426 188 291 197 131 1037 597
Cuts at: 4499 5286 5554 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1037 787 597 426 393 291 268
197 188 131
EcoNI CCTnn'n_nnAGG
Cuts at: 2621 2621
Size: 5839
EcoO109I rG'GnC_Cy
Cuts at: 2666 2666
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3143 3143
Size: 5839
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2207 4986 1678
Size: 529 2779 2531
Fragments arranged by size:
2779 2531 529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 2771 2803 2813
Size: 32 135 32 699 32 468 32 10
Cuts at: 2813 2845 2967 2999 4480 4512 4554 4586 1373
Size: 32 122 32 1481 32 42 32 2626
Fragments arranged by size:
2626 1481 699 468 135 122 42 32
32 32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2531 4953 5137 1305
Size: 1226 2422 184 2007
Fragments arranged by size:
2422 2007 1226 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2918 3797 4156 4315 518
Size: 648 1182 570 879 359 159 2042
Fragments arranged by size:
2042 1182 879 648 570 359 159
MspA1I CmG'CkG
Cuts at: 1606 2325 2498 3255 3932 5080 5325 1606
Size: 719 173 757 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 719 677 245 173
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5287
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2161 3197 4742 1132
Size: 97 932 1036 1545 2229
Fragments arranged by size:
2229 1545 1036 932 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2775 1108
Size: 1667 4172
PfoI T'CCnGG_A
Cuts at: 2711 2711
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PmlI CAC'GTG
Cuts at: 2592 2800 2592
Size: 208 5631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2066 1613
Size: 453 5386
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PspOMI G'GGCC_C
Cuts at: 2666 2666
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2683 3191 830
Size: 1853 508 3478
Fragments arranged by size:
3478 1853 508
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2498 2498
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2990 2990
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2396 2679 3187 4322 5003 5194 826
Size: 219 1351 283 508 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 508 283 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 3943 4844 5106 820
Size: 197 926 293 1707 901 262 1553
Fragments arranged by size:
1707 1553 926 901 293 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2970 3278 513
Size: 552 1905 308 3074
Fragments arranged by size:
3074 1905 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 2331 4085
Size: 274 80 33 51 1443 150 204 1754
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1754 1712 1443 274 204 150 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2214 2246 2803 2835 5799 5831 2214
Size: 32 557 32 2964 32 2222
Fragments arranged by size:
2964 2222 557 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2744 1316
Size: 1428 4411
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI