Restriction map for
pFN21AE2442
(Circular) (Six-base) MAPSORT of: pFN21AE2442.seq Check: 4733 from: 1 to: 10573
pFN21AE2442 10573 bp
With 182 enzymes: SgfI *
February 19, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4726 5413 6079 4726
Size: 687 666 9220
Fragments arranged by size:
9220 687 666
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8383 278
Size: 53 83 186 936 6847 2468
Fragments arranged by size:
6847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7916 1937
Size: 5979 4594
Acc65I G'GTAC_C
Cuts at: 7894 7894
Size: 10573
AclI AA'CG_TT
Cuts at: 1563 8700 9073 1563
Size: 7137 373 3063
Fragments arranged by size:
7137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2720 4783 5748 5872 6703 7487 8636 10020
Size: 1088 2063 965 124 831 784 1149 1384
Cuts at: 10020 10288 1632
Size: 268 1917
Fragments arranged by size:
2063 1917 1384 1149 1088 965 831 784
268 124
AflII C'TTAA_G
Cuts at: 820 1017 5612 6683 820
Size: 197 4595 1071 4710
Fragments arranged by size:
4710 4595 1071 197
AflIII A'CryG_T
Cuts at: 1203 4563 6177 6183 6924 7149 7230 9472 10449
Size: 3360 1614 6 741 225 81 2242 977
Cuts at: 10449 1203
Size: 1327
Fragments arranged by size:
3360 2242 1614 1327 977 741 225 81
6
AgeI A'CCGG_T
Cuts at: 9404 9404
Size: 10573
AhdI GACnn_n'nnGTC
Cuts at: 9302 9302
Size: 10573
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2106 2207 1184
Size: 922 101 9550
Fragments arranged by size:
9550 922 101
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 3239 3271 2443
Size: 32 764 32 9745
Fragments arranged by size:
9745 764 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2684 3100 4809 4838 5153 7571 8316 9888 2684
Size: 416 1709 29 315 2418 745 1572 3369
Fragments arranged by size:
3369 2418 1709 1572 745 416 315 29
ApaI G_GGCC'C
Cuts at: 2332 2719 3826 7029 7872 2332
Size: 387 1107 3203 843 5033
Fragments arranged by size:
5033 3203 1107 843 387
ApaLI G'TGCA_C
Cuts at: 8630 9786 8630
Size: 1156 9417
ApoI r'AATT_y
Cuts at: 1493 2009 6522 7882 8108 8260 10369 1493
Size: 516 4513 1360 226 152 2109 1697
Fragments arranged by size:
4513 2109 1697 1360 516 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9127 160
Size: 872 8095 1606
Fragments arranged by size:
8095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10573
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 3269 3875 6141 7898 1870
Size: 73 382 944 606 2266 1757 4545
Fragments arranged by size:
4545 2266 1757 944 606 382 73
AvrII C'CTAG_G
Cuts at: 4429 7438 4429
Size: 3009 7564
BamHI G'GATC_C
Cuts at: 2021 3994 4752 6780 7903 8270 2021
Size: 1973 758 2028 1123 367 4324
Fragments arranged by size:
4324 2028 1973 1123 758 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5831 7064 7894 9349 618
Size: 325 217 627 4044 1233 830 1455 1842
Fragments arranged by size:
4044 1842 1455 1233 830 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2214 2238 2332 2719 3413 3611 3826 4011
Size: 823 24 94 387 694 198 215 185
Cuts at: 4011 4076 4916 4926 5147 5785 6356 7029 7088
Size: 65 840 10 221 638 571 673 59
Cuts at: 7088 7209 7872 7892 1391
Size: 121 663 20 4072
Fragments arranged by size:
4072 840 823 694 673 663 638 571
387 221 215 198 185 121 94 65
59 24 20 10
BbeI G_GCGC'C
Cuts at: 5835 5835
Size: 10573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2473 3426 3788 4135 4485 4522 5526
Size: 971 574 953 362 347 350 37 1004
Cuts at: 5526 5572 6550 7563 928
Size: 46 978 1013 3938
Fragments arranged by size:
3938 1013 1004 978 971 953 574 362
350 347 46 37
BbvCI CC'TCA_GC
Cuts at: 5004 5229 7004 7650 5004
Size: 225 1775 646 7927
Fragments arranged by size:
7927 1775 646 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8764 8798 1903
Size: 34 6827 34 3678
Fragments arranged by size:
6827 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4547 5383 8465 9675 4547
Size: 836 3082 1210 5445
Fragments arranged by size:
5445 3082 1210 836
BclI T'GATC_A
Cuts at: 1548 2358 6474 1548
Size: 810 4116 5647
Fragments arranged by size:
5647 4116 810
BfrBI ATG'CAT
Cuts at: 5361 5361
Size: 10573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2327 3606 3642
Size: 107 122 71 631 605 655 1279 36
Cuts at: 3642 3830 4492 9184 136
Size: 188 662 4692 1525
Fragments arranged by size:
4692 1525 1279 662 655 631 605 188
122 107 71 36
BglII A'GATC_T
Cuts at: 2623 10568 2623
Size: 7945 2628
BlpI GC'TnA_GC
Cuts at: 5495 7990 5495
Size: 2495 8078
Bme1580I G_kGCm'C
Cuts at: 1790 2332 2719 3826 3899 4363 4998 7029 7644
Size: 542 387 1107 73 464 635 2031 615
Cuts at: 7644 7872 8634 9790 1790
Size: 228 762 1156 2573
Fragments arranged by size:
2573 2031 1156 1107 762 635 615 542
464 387 228 73
BmgBI CAC'GTC
Cuts at: 1318 5196 6981 1318
Size: 3878 1785 4910
Fragments arranged by size:
4910 3878 1785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4373 8304 9262
Size: 416 53 83 399 24 2956 3931 958
Cuts at: 9262 442
Size: 1753
Fragments arranged by size:
3931 2956 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3302 1056
Size: 2246 8327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 3606 3673 3762 4079 4849 4938 5582
Size: 1022 1345 67 89 317 770 89 644
Cuts at: 5582 7190 7407 9233 1239
Size: 1608 217 1826 2579
Fragments arranged by size:
2579 1826 1608 1345 1022 770 644 317
217 89 89 67
Bpu10I CC'TnA_GC
Cuts at: 4839 5004 5229 5310 7004 7650 4839
Size: 165 225 81 1694 646 7762
Fragments arranged by size:
7762 1694 646 225 165 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3887 3950 8698 9563 9861 3887
Size: 63 4748 865 298 4599
Fragments arranged by size:
4748 4599 865 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3582 4993 7235 9236 882
Size: 617 2083 1411 2242 2001 2219
Fragments arranged by size:
2242 2219 2083 2001 1411 617
BsaAI yAC'GTr
Cuts at: 493 1204 2553 8364 493
Size: 711 1349 5811 2702
Fragments arranged by size:
5811 2702 1349 711
BsaBI GATnn'nnATC
Cuts at: 2250 10567 2250
Size: 8317 2256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5832 8380 8762 9397
Size: 53 83 186 936 4299 2548 382 635
Cuts at: 9397 275
Size: 1451
Fragments arranged by size:
4299 2548 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2056 2271 3279 3525 9006 9404 9678 9825 10236
Size: 215 1008 246 5481 398 274 147 411
Cuts at: 10236 2056
Size: 2393
Fragments arranged by size:
5481 2393 1008 411 398 274 246 215
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3239 3269 3671 3701 5234
Size: 30 165 30 1836 30 402 30 1533
Cuts at: 5234 5264 7472 7502 1178
Size: 30 2208 30 4249
Fragments arranged by size:
4249 2208 1836 1533 402 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3221 3893 5242 5849 6329 6992 7208 7496
Size: 2035 672 1349 607 480 663 216 288
Cuts at: 7496 7667 7670 1186
Size: 171 3 4089
Fragments arranged by size:
4089 2035 1349 672 663 607 480 288
216 171 3
BseYI C'CCAG_C
Cuts at: 2163 2215 3619 3674 4234 4821 6990 7087 9776
Size: 52 1404 55 560 587 2169 97 2689
Cuts at: 9776 10272 2163
Size: 496 2464
Fragments arranged by size:
2689 2464 2169 1404 587 560 496 97
55 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 7589 1163
Size: 573 5853 4147
Fragments arranged by size:
5853 4147 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6987 8023 8784 9812 1540
Size: 275 186 4986 1036 761 1028 2301
Fragments arranged by size:
4986 2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 3141 4011 4062 4916 4956 5457 7202 8634
Size: 927 870 51 854 40 501 1745 1432
Cuts at: 8634 8719 9790 2214
Size: 85 1071 2997
Fragments arranged by size:
2997 1745 1432 1071 927 870 854 501
85 51 40
BsmI GAATG_Cn'
Cuts at: 3761 5361 7691 8084 8177 3761
Size: 1600 2330 393 93 6157
Fragments arranged by size:
6157 2330 1600 393 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5186 5186
Size: 10573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2238 2332 2719 3141 3413 3611
Size: 399 424 24 94 387 422 272 198
Cuts at: 3611 3826 3899 4011 4062 4076 4363 4916 4926
Size: 215 73 112 51 14 287 553 10
Cuts at: 4926 4956 4998 5147 5457 5785 6356 7029 7088
Size: 30 42 149 310 328 571 673 59
Cuts at: 7088 7202 7209 7644 7872 7892 8634 8719 9790
Size: 114 7 435 228 20 742 85 1071
Cuts at: 9790 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 673 571 553 435 424
422 399 387 328 310 287 272 228
215 198 149 114 112 94 85 73
59 51 42 30 24 20 14 10
7
BspEI T'CCGG_A
Cuts at: 2056 3279 10236 2056
Size: 1223 6957 2393
Fragments arranged by size:
6957 2393 1223
BspHI T'CATG_A
Cuts at: 3060 5484 5847 6306 6576 6804 8462 10192 3060
Size: 2424 363 459 270 228 1658 1730 3441
Fragments arranged by size:
3441 2424 1730 1658 459 363 270 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4726 4740 4794 5413 6079 7380 7928 844
Size: 3882 14 54 619 666 1301 548 3489
Fragments arranged by size:
3882 3489 1301 666 619 548 54 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 6770 7611 8460 1394
Size: 429 1615 3332 841 849 3507
Fragments arranged by size:
3507 3332 1615 849 841 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2944 3840 4152 4887 4998 6072 6654
Size: 1183 1696 896 312 735 111 1074 582
Cuts at: 6654 7066 7155 9068 9242 65
Size: 412 89 1913 174 1396
Fragments arranged by size:
1913 1696 1396 1183 1074 896 735 582
412 312 174 111 89
BsrFI r'CCGG_y
Cuts at: 1700 1810 6986 9217 9404 1700
Size: 110 5176 2231 187 2869
Fragments arranged by size:
5176 2869 2231 187 110
BsrGI T'GTAC_A
Cuts at: 96 6180 7152 96
Size: 6084 972 3517
Fragments arranged by size:
6084 3517 972
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10573
BssSI C'ACGA_G
Cuts at: 2392 8633 9645 2392
Size: 6241 1012 3320
Fragments arranged by size:
6241 3320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3295 3767 4809 5436 6960 794
Size: 2501 472 1042 627 1524 4407
Fragments arranged by size:
4407 2501 1524 1042 627 472
BstBI TT'CG_AA
Cuts at: 4083 5752 9377 4083
Size: 1669 3625 5279
Fragments arranged by size:
5279 3625 1669
BstEII G'GTnAC_C
Cuts at: 1184 6071 1184
Size: 4887 5686
BstXI CCAn_nnnn'nTGG
Cuts at: 4048 4384 4763 6487 7849 4048
Size: 336 379 1724 1362 6772
Fragments arranged by size:
6772 1724 1362 379 336
BstYI r'GATC_y
Cuts at: 1972 2021 2245 2251 2563 2623 2634 2942 3155
Size: 49 224 6 312 60 11 308 213
Cuts at: 3155 3994 4752 5281 5304 5504 6780 7395 7903
Size: 839 758 529 23 200 1276 615 508
Cuts at: 7903 8270 8655 8672 10124 10210 10222 10568 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1276 839 758 615 529 508
385 367 346 312 308 224 213 200
86 60 49 23 17 12 11 6
Bsu36I CC'TnA_GG
Cuts at: 3101 3555 5217 5286 5297 3101
Size: 454 1662 69 11 8377
Fragments arranged by size:
8377 1662 454 69 11
BtgI C'CryG_G
Cuts at: 513 1065 2004 2629 3195 4053 4063 5405 5544
Size: 552 939 625 566 858 10 1342 139
Cuts at: 5544 6380 7188 7614 513
Size: 836 808 426 3472
Fragments arranged by size:
3472 1342 939 858 836 808 625 566
552 426 139 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4645 4951 487
Size: 683 56 3419 306 6109
Fragments arranged by size:
6109 3419 683 306 56
BtsI GCAGTG_nn'
Cuts at: 2956 3517 4899 5506 5774 6788 7167 7399 8089
Size: 561 1382 607 268 1014 379 232 690
Cuts at: 8089 8883 8903 2956
Size: 794 20 4626
Fragments arranged by size:
4626 1382 1014 794 690 607 561 379
268 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3888 3923 6146 6181 529
Size: 35 3324 35 2223 35 4921
Fragments arranged by size:
4921 3324 2223 35 35 35
DraI TTT'AAA
Cuts at: 6103 7877 6103
Size: 1774 8799
DraIII CAC_nnn'GTG
Cuts at: 2731 4023 2731
Size: 1292 9281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2849 3790 3916 9580 809
Size: 507 1533 941 126 5664 1802
Fragments arranged by size:
5664 1802 1533 941 507 126
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 3535 3634
Size: 54 1425 50 275 18 540 1165 99
Cuts at: 3634 4050 4242 4302 4386 4585 4765 5771 6552
Size: 416 192 60 84 199 180 1006 781
Cuts at: 6552 6745 6771 6984 7185 8020 8909 10538 8
Size: 193 26 213 201 835 889 1629 43
Fragments arranged by size:
1629 1425 1165 1006 889 835 781 540
416 275 213 201 199 193 192 180
99 84 60 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 6984 8020 1537
Size: 275 5172 1036 4090
Fragments arranged by size:
5172 4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2810 3669 5915 6722 6830 6971 8503
Size: 789 668 859 2246 807 108 141 1532
Cuts at: 8503 1353
Size: 3423
Fragments arranged by size:
3423 2246 1532 859 807 789 668 141
108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3044 6719 9160 9534 9680 387
Size: 1064 138 1455 3675 2441 374 146 1280
Fragments arranged by size:
3675 2441 1455 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 4009 4914 2212
Size: 1797 905 7871
Fragments arranged by size:
7871 1797 905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2720 3606 3673 3762 4079 4783
Size: 393 629 459 886 67 89 317 704
Cuts at: 4783 4849 4938 5582 5748 5872 6703 7190 7407
Size: 66 89 644 166 124 831 487 217
Cuts at: 7407 7487 8636 9233 10020 10288 1239
Size: 80 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 886 831 787 704 644 629
597 487 459 393 317 268 217 166
124 89 89 80 67 66
EcoNI CCTnn'n_nnAGG
Cuts at: 7214 7214
Size: 10573
EcoO109I rG'GnC_Cy
Cuts at: 2329 2715 2716 3265 3644 4806 4940 5170 5221
Size: 386 1 549 379 1162 134 230 51
Cuts at: 5221 7025 7026 2329
Size: 1804 1 5876
Fragments arranged by size:
5876 1804 1162 549 386 379 230 134
51 1 1
EcoRI G'AATT_C
Cuts at: 2009 7882 8260 2009
Size: 5873 378 4322
Fragments arranged by size:
5873 4322 378
EcoRV GAT'ATC
Cuts at: 2893 3544 2893
Size: 651 9922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3805 3805
Size: 10573
FspI TGC'GCA
Cuts at: 5429 9079 5429
Size: 3650 6923
HaeII r_GCGC'y
Cuts at: 1678 5835 9720 1678
Size: 4157 3885 2531
Fragments arranged by size:
4157 3885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3308 3340 3413 3445 4997
Size: 32 135 32 1736 32 73 32 1552
Cuts at: 4997 5029 5234 5266 7301 7333 7380 7412 7649
Size: 32 205 32 2035 32 47 32 237
Cuts at: 7649 7681 9214 9246 9288 9320 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 2035 1736 1552 1533 237 205 135
73 47 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 6049 6151 7147 7801 7917
Size: 988 281 1234 2877 102 996 654 116
Cuts at: 7917 8163 669
Size: 246 3079
Fragments arranged by size:
3079 2877 1234 996 988 654 281 246
116 102
HindIII A'AGCT_T
Cuts at: 748 5553 6432 748
Size: 4805 879 4889
Fragments arranged by size:
4889 4805 879
HpaI GTT'AAC
Cuts at: 8163 8163
Size: 10573
KasI G'GCGC_C
Cuts at: 5831 5831
Size: 10573
KpnI G_GTAC'C
Cuts at: 7898 7898
Size: 10573
MfeI C'AATT_G
Cuts at: 8172 8172
Size: 10573
MluI A'CGCG_T
Cuts at: 10449 10449
Size: 10573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5000 7437 9687 9871 1305
Size: 3695 2437 2250 184 2007
Fragments arranged by size:
3695 2437 2250 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3636 4052 4244 4304 4388
Size: 54 1425 343 1804 416 192 60 84
Cuts at: 4388 4587 4767 7187 10
Size: 199 180 2420 3396
Fragments arranged by size:
3396 2420 1804 1425 416 343 199 192
180 84 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3510 3956 4031 4046 4052 5483
Size: 648 1212 1132 446 75 15 6 1431
Cuts at: 5483 5807 5900 6305 6449 6482 6485 6581 6641
Size: 324 93 405 144 33 3 96 60
Cuts at: 6641 7160 7847 8531 8890 9049 518
Size: 519 687 684 359 159 2042
Fragments arranged by size:
2042 1431 1212 1132 687 684 648 519
446 405 359 324 159 144 96 93
75 60 33 15 6 3
MspA1I CmG'CkG
Cuts at: 1606 2167 2191 4491 5101 5460 6131 7989 8666
Size: 561 24 2300 610 359 671 1858 677
Cuts at: 8666 9814 10059 1606
Size: 1148 245 2120
Fragments arranged by size:
2300 2120 1858 1148 677 671 610 561
359 245 24
NaeI GCC'GGC
Cuts at: 6988 6988
Size: 10573
NarI GG'CG_CC
Cuts at: 5832 5832
Size: 10573
NcoI C'CATG_G
Cuts at: 513 1065 2004 3195 4053 4063 5405 7614 513
Size: 552 939 1191 858 10 1342 2209 3472
Fragments arranged by size:
3472 2209 1342 1191 939 858 552 10
NdeI CA'TA_TG
Cuts at: 387 2605 4598 387
Size: 2218 1993 6362
Fragments arranged by size:
6362 2218 1993
NgoMIV G'CCGG_C
Cuts at: 6986 6986
Size: 10573
NheI G'CTAG_C
Cuts at: 1052 3298 1052
Size: 2246 8327
NotI GC'GGCC_GC
Cuts at: 8020 8020
Size: 10573
NsiI A_TGCA'T
Cuts at: 5363 5363
Size: 10573
NspI r_CATG'y
Cuts at: 1132 1229 4501 4567 5806 6157 6187 6928 6958
Size: 97 3272 66 1239 351 30 741 30
Cuts at: 6958 7153 7234 7931 9476 1132
Size: 195 81 697 1545 2229
Fragments arranged by size:
3272 2229 1545 1239 741 697 351 195
97 81 66 30 30
PasI CC'CwG_GG
Cuts at: 1348 4944 5185 7865 1348
Size: 3596 241 2680 4056
Fragments arranged by size:
4056 3596 2680 241
PciI A'CATG_T
Cuts at: 4563 6183 6924 7149 7230 9472 4563
Size: 1620 741 225 81 2242 5664
Fragments arranged by size:
5664 2242 1620 741 225 81
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4311 5153 5655 7405 1108
Size: 3203 842 502 1750 4276
Fragments arranged by size:
4276 3203 1750 842 502
PfoI T'CCnGG_A
Cuts at: 3258 3583 3258
Size: 325 10248
PmeI GTTT'AAAC
Cuts at: 7877 7877
Size: 10573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 8642 8674 10155 10187 3239
Size: 32 5371 32 1481 32 3625
Fragments arranged by size:
5371 3625 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4806 5170 5221 4806
Size: 364 51 10158
Fragments arranged by size:
10158 364 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10573
PsiI TTA'TAA
Cuts at: 8143 8143
Size: 10573
PspOMI G'GGCC_C
Cuts at: 2328 2715 3822 7025 7868 2328
Size: 387 1107 3203 843 5033
Fragments arranged by size:
5033 3203 1107 843 387
PspXI vC'TCGA_Gb
Cuts at: 3875 3875
Size: 10573
PstI C_TGCA'G
Cuts at: 830 4737 5106 6090 7925 830
Size: 3907 369 984 1835 3478
Fragments arranged by size:
3907 3478 1835 984 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10573
PvuII CAG'CTG
Cuts at: 2167 2191 5101 5460 6131 2167
Size: 24 2910 359 671 6609
Fragments arranged by size:
6609 2910 671 359 24
SacI G_AGCT'C
Cuts at: 2214 4011 4916 2214
Size: 1797 905 7871
Fragments arranged by size:
7871 1797 905
SalI G'TCGA_C
Cuts at: 1936 7915 1936
Size: 5979 4594
SapI GCTCTTCn'nnn_
Cuts at: 6722 6722
Size: 10573
SbfI CC_TGCA'GG
Cuts at: 4737 7925 4737
Size: 3188 7385
ScaI AGT'ACT
Cuts at: 5642 6329 6602 8821 10533 5642
Size: 687 273 2219 1712 5682
Fragments arranged by size:
5682 2219 1712 687 273
SexAI A'CCwGG_T
Cuts at: 3165 4638 3165
Size: 1473 9100
SfcI C'TryA_G
Cuts at: 826 1045 2260 3186 4733 5102 6086 7921 9056
Size: 219 1215 926 1547 369 984 1835 1135
Cuts at: 9056 9737 9928 826
Size: 681 191 1471
Fragments arranged by size:
1835 1547 1471 1215 1135 984 926 681
369 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3642 3642
Size: 10573
SfoI GGC'GCC
Cuts at: 5833 5833
Size: 10573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10573
SmaI CCC'GGG
Cuts at: 1872 6143 7900 1872
Size: 4271 1757 4545
Fragments arranged by size:
4545 4271 1757
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3269 3866 3875 3965 5612 6683
Size: 197 926 1326 597 9 90 1647 1071
Cuts at: 6683 8677 9578 9840 820
Size: 1994 901 262 1553
Fragments arranged by size:
1994 1647 1553 1326 1071 926 901 597
262 197 90 9
SnaBI TAC'GTA
Cuts at: 493 8364 493
Size: 7871 2702
SpeI A'CTAG_T
Cuts at: 152 3725 152
Size: 3573 7000
SphI G_CATG'C
Cuts at: 1132 5806 6958 7931 1132
Size: 4674 1152 973 3774
Fragments arranged by size:
4674 3774 1152 973
SspI AAT'ATT
Cuts at: 5 52 5723 8497 5
Size: 47 5671 2774 2081
Fragments arranged by size:
5671 2774 2081 47
StuI AGG'CCT
Cuts at: 1517 2643 2697 3477 7586 7785 1517
Size: 1126 54 780 4109 199 4305
Fragments arranged by size:
4305 4109 1126 780 199 54
StyI C'CwwG_G
Cuts at: 513 1065 2004 2567 2787 3195 4053 4063 4429
Size: 552 939 563 220 408 858 10 366
Cuts at: 4429 4827 4872 5405 6774 7438 7614 8012 513
Size: 398 45 533 1369 664 176 398 3074
Fragments arranged by size:
3074 1369 939 858 664 563 552 533
408 398 398 366 220 176 45 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 3946 5152 5177 5608 5888 8600
Size: 1255 2031 335 1206 25 431 280 2712
Cuts at: 8600 8617 8770 8955 9400 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2712 2031 1498 1255 1206 445 431 335
280 185 153 25 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5565 5640 6180
Size: 274 80 33 51 1443 3588 75 540
Cuts at: 6180 6192 6327 6600 7152 7714 8819 10531 96
Size: 12 135 273 552 562 1105 1712 138
Fragments arranged by size:
3588 1712 1443 1105 562 552 540 274
273 138 135 80 75 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4338 4370 5386 5418 10533 10565 4338
Size: 32 1016 32 5115 32 4346
Fragments arranged by size:
5115 4346 1016 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5576 1316
Size: 4260 6313
XbaI T'CTAG_A
Cuts at: 2248 7909 2248
Size: 5661 4912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 3338 6346 2963
Size: 375 3008 7190
Fragments arranged by size:
7190 3008 375
XhoI C'TCGA_G
Cuts at: 1943 3269 3875 1943
Size: 1326 606 8641
Fragments arranged by size:
8641 1326 606
XmaI C'CCGG_G
Cuts at: 1870 6141 7898 1870
Size: 4271 1757 4545
Fragments arranged by size:
4545 4271 1757
XmnI GAAnn'nnTTC
Cuts at: 3254 3785 4679 5161 5909 6395 6584 8702 3254
Size: 531 894 482 748 486 189 2118 5125
Fragments arranged by size:
5125 2118 894 748 531 486 482 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8381 276
Size: 53 83 186 936 6847 2468
Fragments arranged by size:
6847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BplI BsiWI BstZ17I FseI FspAI
NruI PacI PmlI PsrI RsrII SacII SanDI SrfI
SwaI