Restriction map for
pFN21AE2441
(Circular) (Six-base) MAPSORT of: pFN21AE2441.seq Check: 1973 from: 1 to: 10213
pFN21AE2441 10213 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2338 5710 7236 2338
Size: 3372 1526 5315
Fragments arranged by size:
5315 3372 1526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8023 278
Size: 53 83 186 936 6487 2468
Fragments arranged by size:
6487 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 7556 1937
Size: 616 5003 4594
Fragments arranged by size:
5003 4594 616
Acc65I G'GTAC_C
Cuts at: 7534 7534
Size: 10213
AclI AA'CG_TT
Cuts at: 1563 8340 8713 1563
Size: 6777 373 3063
Fragments arranged by size:
6777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2338 2581 2732 3163 3719 3905 4402 4685
Size: 706 243 151 431 556 186 497 283
Cuts at: 4685 5509 8276 9660 9928 1632
Size: 824 2767 1384 268 1917
Fragments arranged by size:
2767 1917 1384 824 706 556 497 431
283 268 243 186 151
AfeI AGC'GCT
Cuts at: 5006 7238 5006
Size: 2232 7981
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10016
AflIII A'CryG_T
Cuts at: 1203 2898 2970 5814 6777 7260 9112 10089 1203
Size: 1695 72 2844 963 483 1852 977 1327
Fragments arranged by size:
2844 1852 1695 1327 977 963 483 72
AgeI A'CCGG_T
Cuts at: 9044 9044
Size: 10213
AhdI GACnn_n'nnGTC
Cuts at: 8942 8942
Size: 10213
AleI CACnn'nnGTG
Cuts at: 1166 2030 3643 1166
Size: 864 1613 7736
Fragments arranged by size:
7736 1613 864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2048 1184
Size: 864 9349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7100 7132 7100
Size: 32 10181
AlwNI CAG_nnn'CTG
Cuts at: 3997 4844 5073 7448 7956 9528 3997
Size: 847 229 2375 508 1572 4682
Fragments arranged by size:
4682 2375 1572 847 508 229
ApaI G_GGCC'C
Cuts at: 2045 2683 2731 5404 6654 2045
Size: 638 48 2673 1250 5604
Fragments arranged by size:
5604 2673 1250 638 48
ApaLI G'TGCA_C
Cuts at: 2023 8270 9426 2023
Size: 6247 1156 2810
Fragments arranged by size:
6247 2810 1156
ApoI r'AATT_y
Cuts at: 1493 7522 7748 7900 10009 1493
Size: 6029 226 152 2109 1697
Fragments arranged by size:
6029 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8767 160
Size: 872 7735 1606
Fragments arranged by size:
7735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10213
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2538 3562 4577 4628 4652 4724
Size: 73 124 471 1024 1015 51 24 72
Cuts at: 4724 4922 4958 5772 6682 7538 1870
Size: 198 36 814 910 856 4545
Fragments arranged by size:
4545 1024 1015 910 856 814 471 198
124 73 72 51 36 24
AvrII C'CTAG_G
Cuts at: 5464 5464
Size: 10213
BamHI G'GATC_C
Cuts at: 2254 7543 7910 2254
Size: 5289 367 4557
Fragments arranged by size:
5289 4557 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 4774 5468 6686 7009
Size: 325 217 627 245 2742 694 1218 323
Cuts at: 7009 7224 7534 8989 618
Size: 215 310 1455 1842
Fragments arranged by size:
2742 1842 1455 1218 694 627 325 323
310 245 217 215
BanII G_rGCy'C
Cuts at: 1391 2045 2154 2351 2683 2731 3065 3308 3562
Size: 654 109 197 332 48 334 243 254
Cuts at: 3562 4415 4556 5146 5404 6627 6654 6837 7155
Size: 853 141 590 258 1223 27 183 318
Cuts at: 7155 7532 1391
Size: 377 4072
Fragments arranged by size:
4072 1223 853 654 590 377 334 332
318 258 254 243 197 183 141 109
48 27
BbeI G_GCGC'C
Cuts at: 5472 5472
Size: 10213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 2720 2770 3844 4024 4258 4339
Size: 971 383 438 50 1074 180 234 81
Cuts at: 4339 5110 5209 928
Size: 771 99 5932
Fragments arranged by size:
5932 1074 971 771 438 383 234 180
99 81 50
BbvCI CC'TCA_GC
Cuts at: 3959 6956 3959
Size: 2997 7216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8404 8438 1903
Size: 34 6467 34 3678
Fragments arranged by size:
6467 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8105 9315 8105
Size: 1210 9003
BclI T'GATC_A
Cuts at: 1548 2418 1548
Size: 870 9343
BfrBI ATG'CAT
Cuts at: 4237 4237
Size: 10213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3194 3341 3377
Size: 107 122 71 631 605 1522 147 36
Cuts at: 3377 4228 4601 7281 8824 136
Size: 851 373 2680 1543 1525
Fragments arranged by size:
2680 1543 1525 1522 851 631 605 373
147 122 107 71 36
BglII A'GATC_T
Cuts at: 10208 10208
Size: 10213
BlpI GC'TnA_GC
Cuts at: 2345 4409 4571 5132 7630 2345
Size: 2064 162 561 2498 4928
Fragments arranged by size:
4928 2498 2064 561 162
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2045 2683 2731 3108 3638 4779 5404
Size: 237 18 638 48 377 530 1141 625
Cuts at: 5404 5422 6654 6689 8274 9430 1790
Size: 18 1232 35 1585 1156 2573
Fragments arranged by size:
2573 1585 1232 1156 1141 638 625 530
377 237 48 35 18 18
BmgBI CAC'GTC
Cuts at: 1318 6606 1318
Size: 5288 4925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6895 7944 8902
Size: 416 53 83 399 24 5478 1049 958
Cuts at: 8902 442
Size: 1753
Fragments arranged by size:
5478 1753 1049 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 3077 3470 4626 4823 6695 6818 8873
Size: 1025 813 393 1156 197 1872 123 2055
Cuts at: 8873 1239
Size: 2579
Fragments arranged by size:
2579 2055 1872 1156 1025 813 393 197
123
Bpu10I CC'TnA_GC
Cuts at: 2786 3959 4550 6956 2786
Size: 1173 591 2406 6043
Fragments arranged by size:
6043 2406 1173 591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3440 6128 6194 7079 7154 8338 9203 9501 3440
Size: 2688 66 885 75 1184 865 298 4152
Fragments arranged by size:
4152 2688 1184 885 865 298 75 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 4289 5099 6557 8876 882
Size: 617 706 2084 810 1458 2319 2219
Fragments arranged by size:
2319 2219 2084 1458 810 706 617
BsaAI yAC'GTr
Cuts at: 493 1204 2883 2901 2973 5023 8004 493
Size: 711 1679 18 72 2050 2981 2702
Fragments arranged by size:
2981 2702 2050 1679 711 72 18
BsaBI GATnn'nnATC
Cuts at: 2423 4478 5747 6089 6179 7430 10207 2423
Size: 2055 1269 342 90 1251 2777 2429
Fragments arranged by size:
2777 2429 2055 1269 1251 342 90
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3021 3600 5469 5880
Size: 53 83 186 936 1488 579 1869 411
Cuts at: 5880 6894 7089 7296 7404 8020 8402 9037 275
Size: 1014 195 207 108 616 382 635 1451
Fragments arranged by size:
1869 1488 1451 1014 936 635 616 579
411 382 207 195 186 108 83 53
BsaWI w'CCGG_w
Cuts at: 6408 8646 9044 9318 9465 9876 6408
Size: 2238 398 274 147 411 6745
Fragments arranged by size:
6745 2238 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4178 4208 7100 7130 1178
Size: 30 165 30 2775 30 2892 30 4261
Fragments arranged by size:
4261 2892 2775 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2123 2162 3413 3416 3458 4670 4805
Size: 934 3 39 1251 3 42 1212 135
Cuts at: 4805 4832 4940 4952 4964 4991 5798 6455 6458
Size: 27 108 12 12 27 807 657 3
Cuts at: 6458 6617 6836 7124 7199 7397 1186
Size: 159 219 288 75 198 4002
Fragments arranged by size:
4002 1251 1212 934 807 657 288 219
198 159 135 108 75 42 39 27
27 12 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2991 3101 3183 3502 4102 5353 5368 6615 9416
Size: 110 82 319 600 1251 15 1247 2801
Cuts at: 9416 9912 2991
Size: 496 3292
Fragments arranged by size:
3292 2801 1251 1247 600 496 319 110
82 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5021 1163
Size: 573 3285 6355
Fragments arranged by size:
6355 3285 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2721 6612 7663 8424 9452 1540
Size: 275 186 720 3891 1051 761 1028 2301
Fragments arranged by size:
3891 2301 1051 1028 761 720 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 2388 3672 3774 3819 3834 4415 5094 6210
Size: 361 1284 102 45 15 581 679 1116
Cuts at: 6210 8274 8359 9430 2027
Size: 2064 85 1071 2810
Fragments arranged by size:
2810 2064 1284 1116 1071 679 581 361
102 85 45 15
BsmI GAATG_Cn'
Cuts at: 7724 7817 7724
Size: 93 10120
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2045 2154 2351 2388 2683 2731
Size: 399 237 18 109 197 37 295 48
Cuts at: 2731 3065 3108 3308 3562 3638 3672 3774 3819
Size: 334 43 200 254 76 34 102 45
Cuts at: 3819 3834 4415 4556 4779 5094 5146 5404 5422
Size: 15 581 141 223 315 52 258 18
Cuts at: 5422 6210 6627 6654 6689 6837 7155 7532 8274
Size: 788 417 27 35 148 318 377 742
Cuts at: 8274 8359 9430 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 788 742 581 417 399 377
334 318 315 295 258 254 237 223
200 197 148 141 109 102 85 76
52 48 45 43 37 35 34 27
18 18 15
BspEI T'CCGG_A
Cuts at: 6408 9876 6408
Size: 3468 6745
BspHI T'CATG_A
Cuts at: 2415 2445 2607 3210 5121 5484 6081 6429 8102
Size: 30 162 603 1911 363 597 348 1673
Cuts at: 8102 9832 2415
Size: 1730 2796
Fragments arranged by size:
2796 1911 1730 1673 603 597 363 348
162 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2349 4227 4476 5710 7236 7568 844
Size: 1494 11 1878 249 1234 1526 332 3489
Fragments arranged by size:
3489 1878 1526 1494 1234 332 249 11
BsrBI CCG'CTC
Cuts at: 1394 1823 8100 1394
Size: 429 6277 3507
Fragments arranged by size:
6277 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2899 2917 2935 2953 3529 8708 8882
Size: 1183 1651 18 18 18 576 5179 174
Cuts at: 8882 65
Size: 1396
Fragments arranged by size:
5179 1651 1396 1183 576 174 18 18
18
BsrFI r'CCGG_y
Cuts at: 1700 1810 4322 5552 6611 7221 8857 9044 1700
Size: 110 2512 1230 1059 610 1636 187 2869
Fragments arranged by size:
2869 2512 1636 1230 1059 610 187 110
BsrGI T'GTAC_A
Cuts at: 96 6159 6780 96
Size: 6063 621 3529
Fragments arranged by size:
6063 3529 621
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10213
BssSI C'ACGA_G
Cuts at: 6050 7181 7349 8273 9285 6050
Size: 1131 168 924 1012 6978
Fragments arranged by size:
6978 1131 1012 924 168
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3519 4278 5073 6585 7483 794
Size: 2725 759 795 1512 898 3524
Fragments arranged by size:
3524 2725 1512 898 795 759
BstBI TT'CG_AA
Cuts at: 9017 9017
Size: 10213
BstEII G'GTnAC_C
Cuts at: 1184 4106 6106 1184
Size: 2922 2000 5291
Fragments arranged by size:
5291 2922 2000
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 3645 5042 5353 6118 6796 7355 2482
Size: 1163 1397 311 765 678 559 5340
Fragments arranged by size:
5340 1397 1163 765 678 559 311
BstYI r'GATC_y
Cuts at: 1972 2254 2502 4545 6027 7023 7203 7543 7910
Size: 282 248 2043 1482 996 180 340 367
Cuts at: 7910 8295 8312 9764 9850 9862 10208 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2043 1977 1482 1452 996 385 367 346
340 282 248 180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2657 2831 3114 4845 4935 4974 5414 2657
Size: 174 283 1731 90 39 440 7456
Fragments arranged by size:
7456 1731 440 283 174 90 39
BtgI C'CryG_G
Cuts at: 513 1065 2004 3336 3345 3508 3603 3641 3692
Size: 552 939 1332 9 163 95 38 51
Cuts at: 3692 3792 3897 4149 5041 5763 6391 7005 7104
Size: 100 105 252 892 722 628 614 99
Cuts at: 7104 513
Size: 3622
Fragments arranged by size:
3622 1332 939 892 722 628 614 552
252 163 105 100 99 95 51 38
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3525 4765 6769 6892 487
Size: 683 56 2299 1240 2004 123 3808
Fragments arranged by size:
3808 2299 2004 1240 683 123 56
BtsI GCAGTG_nn'
Cuts at: 3100 3106 5143 5393 7027 7446 7729 8523 8543
Size: 6 2037 250 1634 419 283 794 20
Cuts at: 8543 3100
Size: 4770
Fragments arranged by size:
4770 2037 1634 794 419 283 250 20
6
ClaI AT'CG_AT
Cuts at: 1554 6543 1554
Size: 4989 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10178
DraI TTT'AAA
Cuts at: 7517 7517
Size: 10213
DraIII CAC_nnn'GTG
Cuts at: 2171 2883 3667 2171
Size: 712 784 8717
Fragments arranged by size:
8717 784 712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 6269 9220 809
Size: 507 1284 3669 2951 1802
Fragments arranged by size:
3669 2951 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3333 3342
Size: 54 1425 50 275 18 177 1326 9
Cuts at: 3342 3378 3789 3981 4041 4052 4320 4324 5343
Size: 36 411 192 60 11 268 4 1019
Cuts at: 5343 5724 6609 6813 7345 7660 8549 10178 8
Size: 381 885 204 532 315 889 1629 43
Fragments arranged by size:
1629 1425 1326 1019 889 885 532 411
381 315 275 268 204 192 177 60
54 50 43 36 18 11 9 4
EagI C'GGCC_G
Cuts at: 1537 1812 6609 7660 1537
Size: 275 4797 1051 4090
Fragments arranged by size:
4797 4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 3206 3242 3612 3788 5876 6455 6476
Size: 1469 384 36 370 176 2088 579 21
Cuts at: 6476 8143 1353
Size: 1667 3423
Fragments arranged by size:
3423 2088 1667 1469 579 384 370 176
36 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2311 3157 3194 6344 8800 9174
Size: 1064 138 722 846 37 3150 2456 374
Cuts at: 9174 9320 387
Size: 146 1280
Fragments arranged by size:
3150 2456 1280 1064 846 722 374 146
138 37
EcoICRI GAG'CTC
Cuts at: 4413 4413
Size: 10213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2338 2581 2732 3077 3163 3470
Size: 393 632 74 243 151 345 86 307
Cuts at: 3470 3719 3905 4402 4626 4685 4823 5509 6695
Size: 249 186 497 224 59 138 686 1186
Cuts at: 6695 6818 8276 8873 9660 9928 1239
Size: 123 1458 597 787 268 1524
Fragments arranged by size:
1524 1458 1186 787 686 632 597 497
393 345 307 268 249 243 224 186
151 138 123 86 74 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3380 4580 4667 5590 5717 7470 3380
Size: 1200 87 923 127 1753 6123
Fragments arranged by size:
6123 1753 1200 923 127 87
EcoO109I rG'GnC_Cy
Cuts at: 2042 2248 2575 2661 2680 2727 2728 3499 4660
Size: 206 327 86 19 47 1 771 1161
Cuts at: 4660 4930 5070 5372 5401 6651 7063 7212 2042
Size: 270 140 302 29 1250 412 149 5043
Fragments arranged by size:
5043 1250 1161 771 412 327 302 270
206 149 140 86 47 29 19 1
EcoRI G'AATT_C
Cuts at: 7522 7900 7522
Size: 378 9835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2326 4222 5296 6082 2296
Size: 30 1896 1074 786 6427
Fragments arranged by size:
6427 1896 1074 786 30
FseI GG_CCGG'CC
Cuts at: 4326 4326
Size: 10213
FspI TGC'GCA
Cuts at: 5066 8719 5066
Size: 3653 6560
HaeII r_GCGC'y
Cuts at: 1678 4103 4425 5008 5472 7240 9360 1678
Size: 2425 322 583 464 1768 2120 2531
Fragments arranged by size:
2531 2425 2120 1768 583 464 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2799 2831 4820 4852 4871
Size: 32 135 32 1227 32 1989 32 19
Cuts at: 4871 4903 8854 8886 8928 8960 1373
Size: 32 3951 32 42 32 2626
Fragments arranged by size:
3951 2626 1989 1227 135 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 5542 5614 5680 6775 7557
Size: 988 281 616 2988 72 66 1095 782
Cuts at: 7557 7803 669
Size: 246 3079
Fragments arranged by size:
3079 2988 1095 988 782 616 281 246
72 66
HindIII A'AGCT_T
Cuts at: 748 5978 748
Size: 5230 4983
HpaI GTT'AAC
Cuts at: 7803 7803
Size: 10213
KasI G'GCGC_C
Cuts at: 5468 5468
Size: 10213
KpnI G_GTAC'C
Cuts at: 7538 7538
Size: 10213
MfeI C'AATT_G
Cuts at: 7812 7812
Size: 10213
MluI A'CGCG_T
Cuts at: 10089 10089
Size: 10213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2736 3090 3621 3908 4821 5720 6534 9327
Size: 1431 354 531 287 913 899 814 2793
Cuts at: 9327 9511 1305
Size: 184 2007
Fragments arranged by size:
2793 2007 1431 913 899 814 531 354
287 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3344 3791 3983 4043
Size: 54 1425 343 177 1335 447 192 60
Cuts at: 4043 4054 5345 5726 6815 7347 10
Size: 11 1291 381 1089 532 2876
Fragments arranged by size:
2876 1425 1335 1291 1089 532 447 381
343 192 177 60 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 2020 2030 2381 2450 2609 3643 3791
Size: 648 854 10 351 69 159 1034 148
Cuts at: 3791 4236 4316 4358 4772 5240 5444 5489 5936
Size: 445 80 42 414 468 204 45 447
Cuts at: 5936 6080 6113 6116 6170 7353 8171 8530 8689
Size: 144 33 3 54 1183 818 359 159
Cuts at: 8689 518
Size: 2042
Fragments arranged by size:
2042 1183 1034 854 818 648 468 447
445 414 359 351 204 159 159 148
144 80 69 54 45 42 33 10
3
MspA1I CmG'CkG
Cuts at: 1606 2095 2125 3183 3244 3260 3579 3588 4165
Size: 489 30 1058 61 16 319 9 577
Cuts at: 4165 4907 5176 5661 6393 6648 6955 7287 7629
Size: 742 269 485 732 255 307 332 342
Cuts at: 7629 8306 9454 9699 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1058 742 732 677 577 489
485 342 332 319 307 269 255 245
61 30 16 9
NaeI GCC'GGC
Cuts at: 4324 5554 6613 7223 4324
Size: 1230 1059 610 7314
Fragments arranged by size:
7314 1230 1059 610
NarI GG'CG_CC
Cuts at: 5469 5469
Size: 10213
NcoI C'CATG_G
Cuts at: 513 1065 2004 3345 3508 3603 3641 3792 3897
Size: 552 939 1341 163 95 38 151 105
Cuts at: 3897 7005 7104 513
Size: 3108 99 3622
Fragments arranged by size:
3622 3108 1341 939 552 163 151 105
99 95 38
NdeI CA'TA_TG
Cuts at: 387 3352 3674 387
Size: 2965 322 6926
Fragments arranged by size:
6926 2965 322
NgoMIV G'CCGG_C
Cuts at: 4322 5552 6611 7221 4322
Size: 1230 1059 610 7314
Fragments arranged by size:
7314 1230 1059 610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10213
NotI GC'GGCC_GC
Cuts at: 7660 7660
Size: 10213
NsiI A_TGCA'T
Cuts at: 4239 4239
Size: 10213
NspI r_CATG'y
Cuts at: 1132 1229 2410 4069 4162 4237 5818 6508 6781
Size: 97 1181 1659 93 75 1581 690 273
Cuts at: 6781 7264 7571 9116 1132
Size: 483 307 1545 2229
Fragments arranged by size:
2229 1659 1581 1545 1181 690 483 307
273 97 93 75
PasI CC'CwG_GG
Cuts at: 1348 2705 5035 7472 1348
Size: 1357 2330 2437 4089
Fragments arranged by size:
4089 2437 2330 1357
PciI A'CATG_T
Cuts at: 5814 6777 7260 9112 5814
Size: 963 483 1852 6915
Fragments arranged by size:
6915 1852 963 483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3475 3509 4050 5292 6037 7033 1108
Size: 2367 34 541 1242 745 996 4288
Fragments arranged by size:
4288 2367 1242 996 745 541 34
PfoI T'CCnGG_A
Cuts at: 2571 3054 6795 2571
Size: 483 3741 5989
Fragments arranged by size:
5989 3741 483
PmeI GTTT'AAAC
Cuts at: 7517 7517
Size: 10213
PmlI CAC'GTG
Cuts at: 2883 2901 2973 2883
Size: 18 72 10123
Fragments arranged by size:
10123 72 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7100 7132 7352 7384 8282 8314 9795 9827 7100
Size: 32 220 32 898 32 1481 32 7486
Fragments arranged by size:
7486 1481 898 220 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2575 2661 3499 4660 4930 5372 7063 2248
Size: 327 86 838 1161 270 442 1691 5398
Fragments arranged by size:
5398 1691 1161 838 442 327 270 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10213
PsiI TTA'TAA
Cuts at: 7783 7783
Size: 10213
PspOMI G'GGCC_C
Cuts at: 2041 2679 2727 5400 6650 2041
Size: 638 48 2673 1250 5604
Fragments arranged by size:
5604 2673 1250 638 48
PspXI vC'TCGA_Gb
Cuts at: 4724 4724
Size: 10213
PstI C_TGCA'G
Cuts at: 830 2124 2814 4473 4901 5721 6743 6948 7565
Size: 1294 690 1659 428 820 1022 205 617
Cuts at: 7565 830
Size: 3478
Fragments arranged by size:
3478 1659 1294 1022 820 690 617 428
205
PvuI CG_AT'CG
Cuts at: 2001 2721 2001
Size: 720 9493
PvuII CAG'CTG
Cuts at: 3183 3244 3260 4165 4907 3183
Size: 61 16 905 742 8489
Fragments arranged by size:
8489 905 742 61 16
SacI G_AGCT'C
Cuts at: 4415 4415
Size: 10213
SacII CC_GC'GG
Cuts at: 6394 6394
Size: 10213
SalI G'TCGA_C
Cuts at: 1936 2552 7555 1936
Size: 616 5003 4594
Fragments arranged by size:
5003 4594 616
SanDI GG'GwC_CC
Cuts at: 2575 3499 5372 2575
Size: 924 1873 7416
Fragments arranged by size:
7416 1873 924
SapI GCTCTTCn'nnn_
Cuts at: 2822 2822
Size: 10213
SbfI CC_TGCA'GG
Cuts at: 5721 6948 7565 5721
Size: 1227 617 8369
Fragments arranged by size:
8369 1227 617
ScaI AGT'ACT
Cuts at: 6233 6323 8461 10173 6233
Size: 90 2138 1712 6273
Fragments arranged by size:
6273 2138 1712 90
SexAI A'CCwGG_T
Cuts at: 3846 3846
Size: 10213
SfcI C'TryA_G
Cuts at: 826 1045 2120 2263 2810 4469 4897 5717 6739
Size: 219 1075 143 547 1659 428 820 1022
Cuts at: 6739 6913 6944 7232 7561 8696 9377 9568 826
Size: 174 31 288 329 1135 681 191 1471
Fragments arranged by size:
1659 1471 1135 1075 1022 820 681 547
428 329 288 219 191 174 143 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 3341 3377 3341
Size: 36 10177
SfoI GGC'GCC
Cuts at: 5470 5470
Size: 10213
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10213
SmaI CCC'GGG
Cuts at: 1872 2069 5774 6684 7540 1872
Size: 197 3705 910 856 4545
Fragments arranged by size:
4545 3705 910 856 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3419 4724 6143 6209 7094 7169
Size: 197 926 1476 1305 1419 66 885 75
Cuts at: 7169 8317 9218 9480 820
Size: 1148 901 262 1553
Fragments arranged by size:
1553 1476 1419 1305 1148 926 901 885
262 197 75 66
SnaBI TAC'GTA
Cuts at: 493 8004 493
Size: 7511 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10213
SphI G_CATG'C
Cuts at: 1132 4162 7571 1132
Size: 3030 3409 3774
Fragments arranged by size:
3774 3409 3030
SspI AAT'ATT
Cuts at: 5 52 8137 5
Size: 47 8085 2081
Fragments arranged by size:
8085 2081 47
StuI AGG'CCT
Cuts at: 1517 3978 4517 4994 6065 1517
Size: 2461 539 477 1071 5665
Fragments arranged by size:
5665 2461 1071 539 477
StyI C'CwwG_G
Cuts at: 513 1065 2004 3345 3459 3508 3603 3641 3792
Size: 552 939 1341 114 49 95 38 151
Cuts at: 3792 3897 4206 4512 4557 5464 6399 6838 7005
Size: 105 309 306 45 907 935 439 167
Cuts at: 7005 7104 7137 7652 513
Size: 99 33 515 3074
Fragments arranged by size:
3074 1341 939 935 907 552 515 439
309 306 167 151 114 105 99 95
49 45 38 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5245 5525 6349 6914 7058 8240 8257
Size: 1255 3665 280 824 565 144 1182 17
Cuts at: 8257 8410 8595 9040 325
Size: 153 185 445 1498
Fragments arranged by size:
3665 1498 1255 1182 824 565 445 280
185 153 144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 5277 6159
Size: 274 80 33 51 1443 510 2790 882
Cuts at: 6159 6231 6321 6780 8459 10171 96
Size: 72 90 459 1679 1712 138
Fragments arranged by size:
2790 1712 1679 1443 882 510 459 274
138 90 80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3079 3111 3987 4019 4178 4210 6358 6390 10173
Size: 32 876 32 159 32 2148 32 3783
Cuts at: 10173 10205 3079
Size: 32 3087
Fragments arranged by size:
3783 3087 2148 876 159 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5213 1316
Size: 3897 6316
XbaI T'CTAG_A
Cuts at: 7549 7549
Size: 10213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3610 4726 5335 7063 3610
Size: 1116 609 1728 6760
Fragments arranged by size:
6760 1728 1116 609
XhoI C'TCGA_G
Cuts at: 1943 4724 1943
Size: 2781 7432
XmaI C'CCGG_G
Cuts at: 1870 2067 5772 6682 7538 1870
Size: 197 3705 910 856 4545
Fragments arranged by size:
4545 3705 910 856 197
XmnI GAAnn'nnTTC
Cuts at: 2279 3236 3935 4391 6323 8342 2279
Size: 957 699 456 1932 2019 4150
Fragments arranged by size:
4150 2019 1932 957 699 456
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8021 276
Size: 53 83 186 936 6487 2468
Fragments arranged by size:
6487 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BplI BsiWI BsmBI BstZ17I EcoRV FspAI
NruI PacI PsrI RsrII SrfI SwaI