Restriction map for
pFN21AE2440
(Circular) (Six-base) MAPSORT of: pFN21AE2440.seq Check: 9289 from: 1 to: 13930
pFN21AE2440 13930 bp
With 182 enzymes: SgfI *
August 14, 2013 14:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4427 6275 7353 10028 11143 4427
Size: 1848 1078 2675 1115 7214
Fragments arranged by size:
7214 2675 1848 1115 1078
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3834 5297 9908 11740
Size: 53 83 186 936 2298 1463 4611 1832
Cuts at: 11740 278
Size: 2468
Fragments arranged by size:
4611 2468 2298 1832 1463 936 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3804 5156 7599 7608 10008 10443 11024 11273
Size: 1867 1352 2443 9 2400 435 581 249
Cuts at: 11273 1937
Size: 4594
Fragments arranged by size:
4594 2443 2400 1867 1352 581 435 249
9
Acc65I G'GTAC_C
Cuts at: 2925 6141 9731 11251 2925
Size: 3216 3590 1520 5604
Fragments arranged by size:
5604 3590 3216 1520
AclI AA'CG_TT
Cuts at: 1563 3432 12057 12430 1563
Size: 1869 8625 373 3063
Fragments arranged by size:
8625 3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4637 5735 7064 7200 8028 11155 11993 13377
Size: 3005 1098 1329 136 828 3127 838 1384
Cuts at: 13377 13645 1632
Size: 268 1917
Fragments arranged by size:
3127 3005 1917 1384 1329 1098 838 828
268 136
AfeI AGC'GCT
Cuts at: 2626 2626
Size: 13930
AflII C'TTAA_G
Cuts at: 820 1017 2341 820
Size: 197 1324 12409
Fragments arranged by size:
12409 1324 197
AflIII A'CryG_T
Cuts at: 1203 2972 7396 7578 9625 9673 12829 13806 1203
Size: 1769 4424 182 2047 48 3156 977 1327
Fragments arranged by size:
4424 3156 2047 1769 1327 977 182 48
AgeI A'CCGG_T
Cuts at: 12761 12761
Size: 13930
AhdI GACnn_n'nnGTC
Cuts at: 12659 12659
Size: 13930
AleI CACnn'nnGTG
Cuts at: 1166 5640 1166
Size: 4474 9456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 5658 7170 7361 10091 1184
Size: 1291 3183 1512 191 2730 5023
Fragments arranged by size:
5023 3183 2730 1512 1291 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10493 10525 10493
Size: 32 13898
AlwNI CAG_nnn'CTG
Cuts at: 2448 2916 3691 4529 5036 7481 7738 8110 8173
Size: 468 775 838 507 2445 257 372 63
Cuts at: 8173 11673 13245 2448
Size: 3500 1572 3133
Fragments arranged by size:
3500 3133 2445 1572 838 775 507 468
372 257 63
ApaI G_GGCC'C
Cuts at: 3414 3414
Size: 13930
ApaLI G'TGCA_C
Cuts at: 11987 13143 11987
Size: 1156 12774
ApoI r'AATT_y
Cuts at: 1493 2654 4215 5207 5878 6771 7197 7376 8229
Size: 1161 1561 992 671 893 426 179 853
Cuts at: 8229 9421 9885 11239 11465 11617 13726 1493
Size: 1192 464 1354 226 152 2109 1697
Fragments arranged by size:
2109 1697 1561 1354 1192 1161 992 893
853 671 464 426 226 179 152
AseI AT'TA_AT
Cuts at: 160 1032 12484 160
Size: 872 11452 1606
Fragments arranged by size:
11452 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13930
AvaI C'yCGr_G
Cuts at: 1870 1943 3495 3719 4347 4465 6607 10086 11255
Size: 73 1552 224 628 118 2142 3479 1169
Cuts at: 11255 1870
Size: 4545
Fragments arranged by size:
4545 3479 2142 1552 1169 628 224 118
73
AvrII C'CTAG_G
Cuts at: 10516 10516
Size: 13930
BamHI G'GATC_C
Cuts at: 2752 11260 11627 2752
Size: 8508 367 5055
Fragments arranged by size:
8508 5055 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2267 2432 2572 2925 3648
Size: 325 217 627 480 165 140 353 723
Cuts at: 3648 4640 5124 5408 5753 6141 7551 9731 11251
Size: 992 484 284 345 388 1410 2180 1520
Cuts at: 11251 12706 618
Size: 1455 1842
Fragments arranged by size:
2180 1842 1520 1455 1410 992 723 627
484 480 388 353 345 325 284 217
165 140
BanII G_rGCy'C
Cuts at: 1391 3402 3414 4764 5525 6615 7431 8555 8669
Size: 2011 12 1350 761 1090 816 1124 114
Cuts at: 8669 9459 10086 10902 11150 11249 1391
Size: 790 627 816 248 99 4072
Fragments arranged by size:
4072 2011 1350 1124 1090 816 816 790
761 627 248 114 99 12
BbeI G_GCGC'C
Cuts at: 3652 4644 3652
Size: 992 12938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 8922 928
Size: 971 7023 5936
Fragments arranged by size:
7023 5936 971
BbvCI CC'TCA_GC
Cuts at: 3873 5945 7296 7317 7617 7877 8081 3873
Size: 2072 1351 21 300 260 204 9722
Fragments arranged by size:
9722 2072 1351 300 260 204 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3950 3984 11164 11198 12121
Size: 34 50 34 1929 34 7180 34 923
Cuts at: 12121 12155 1903
Size: 34 3678
Fragments arranged by size:
7180 3678 1929 923 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8624 11822 13032 8624
Size: 3198 1210 9522
Fragments arranged by size:
9522 3198 1210
BclI T'GATC_A
Cuts at: 1548 3603 4014 8319 1548
Size: 2055 411 4305 7159
Fragments arranged by size:
7159 4305 2055 411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2179 2944 3333
Size: 107 122 71 631 605 507 765 389
Cuts at: 3333 3722 4179 12541 136
Size: 389 457 8362 1525
Fragments arranged by size:
8362 1525 765 631 605 507 457 389
389 122 107 71
BglII A'GATC_T
Cuts at: 2713 13925 2713
Size: 11212 2718
BlpI GC'TnA_GC
Cuts at: 6337 8099 10226 11347 6337
Size: 1762 2127 1121 8920
Fragments arranged by size:
8920 2127 1762 1121
Bme1580I G_kGCm'C
Cuts at: 1790 3287 3414 4465 4957 5100 5411 5573 9633
Size: 1497 127 1051 492 143 311 162 4060
Cuts at: 9633 9836 11991 13147 1790
Size: 203 2155 1156 2573
Fragments arranged by size:
4060 2573 2155 1497 1156 1051 492 311
203 162 143 127
BmgBI CAC'GTC
Cuts at: 1318 7261 8052 8089 8224 1318
Size: 5943 791 37 135 7024
Fragments arranged by size:
7024 5943 791 135 37
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3371 3718
Size: 416 53 83 399 24 912 1042 347
Cuts at: 3718 5110 5504 10528 11014 11661 12619 442
Size: 1392 394 5024 486 647 958 1753
Fragments arranged by size:
5024 1753 1392 1042 958 912 647 486
416 399 394 347 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 9209 11135 1056
Size: 8153 1926 3851
Fragments arranged by size:
8153 3851 1926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3711 4084 5708 6173 9726 3711
Size: 373 1624 465 3553 7915
Fragments arranged by size:
7915 3553 1624 465 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2962 5058 5336 5510 6791 7834 9473 10145
Size: 1723 2096 278 174 1281 1043 1639 672
Cuts at: 10145 10231 10286 10778 12590 1239
Size: 86 55 492 1812 2579
Fragments arranged by size:
2579 2096 1812 1723 1639 1281 1043 672
492 278 174 86 55
Bpu10I CC'TnA_GC
Cuts at: 2726 3873 5945 7296 7317 7617 7877 8081 10041
Size: 1147 2072 1351 21 300 260 204 1960
Cuts at: 10041 2726
Size: 6615
Fragments arranged by size:
6615 2072 1960 1351 1147 300 260 204
21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 4772 5584 5757 6466 7154 12055 12920 13218
Size: 2172 812 173 709 688 4901 865 298
Cuts at: 13218 2600
Size: 3312
Fragments arranged by size:
4901 3312 2172 865 812 709 688 298
173
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 5330 5679 10160 10366 12593 882
Size: 617 3249 582 349 4481 206 2227 2219
Fragments arranged by size:
4481 3249 2227 2219 617 582 349 206
BsaAI yAC'GTr
Cuts at: 493 1204 5500 9628 11721 493
Size: 711 4296 4128 2093 2702
Fragments arranged by size:
4296 4128 2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 7004 13924 7004
Size: 6920 7010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3649 3831 4302 4641
Size: 53 83 186 936 2116 182 471 339
Cuts at: 4641 5294 5304 5748 6435 9905 10785 11737 12119
Size: 653 10 444 687 3470 880 952 382
Cuts at: 12119 12754 275
Size: 635 1451
Fragments arranged by size:
3470 2116 1451 952 936 880 687 653
635 471 444 382 339 186 182 83
53 10
BsaWI w'CCGG_w
Cuts at: 3790 6615 9189 9462 12363 12761 13035 13182 13593
Size: 2825 2574 273 2901 398 274 147 411
Cuts at: 13593 3790
Size: 4127
Fragments arranged by size:
4127 2901 2825 2574 411 398 274 273
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3066 3096 4428 4458 5031
Size: 30 165 30 1663 30 1332 30 573
Cuts at: 5031 5061 5108 5138 8537 8567 10493 10523 1178
Size: 30 47 30 3399 30 1926 30 4585
Fragments arranged by size:
4585 3399 1926 1663 1332 573 165 47
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3087 3090 6469 8294 10762 1186
Size: 1901 3 3379 1825 2468 4354
Fragments arranged by size:
4354 3379 2468 1901 1825 3
BseYI C'CCAG_C
Cuts at: 2291 5518 6160 6598 13133 13629 2291
Size: 3227 642 438 6535 496 2592
Fragments arranged by size:
6535 3227 2592 642 496 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2533 2649 3388 4518 4876 5664 6035
Size: 573 797 116 739 1130 358 788 371
Cuts at: 6035 7452 7728 7859 9701 10008 10292 10356 11174
Size: 1417 276 131 1842 307 284 64 818
Cuts at: 11174 11177 1163
Size: 3 3916
Fragments arranged by size:
3916 1842 1417 1130 818 797 788 739
573 371 358 307 284 276 131 116
64 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5577 7836 10450 11380 12141 13169
Size: 275 186 3576 2259 2614 930 761 1028
Cuts at: 13169 1540
Size: 2301
Fragments arranged by size:
3576 2614 2301 2259 1028 930 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2022 4128 6355 6479 7431 7503 8078 8669 10302
Size: 2106 2227 124 952 72 575 591 1633
Cuts at: 10302 10920 11991 12076 13147 2022
Size: 618 1071 85 1071 2805
Fragments arranged by size:
2805 2227 2106 1633 1071 1071 952 618
591 575 124 85 72
BsmI GAATG_Cn'
Cuts at: 2295 4238 4718 7702 7954 9754 11441 11534 2295
Size: 1943 480 2984 252 1800 1687 93 4691
Fragments arranged by size:
4691 2984 1943 1800 1687 480 252 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3630 3791 4072 7784 3630
Size: 161 281 3712 9776
Fragments arranged by size:
9776 3712 281 161
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 3287 3402 3414 4128 4465 4764
Size: 399 232 1265 115 12 714 337 299
Cuts at: 4764 4957 5100 5411 5525 5573 6355 6479 6615
Size: 193 143 311 114 48 782 124 136
Cuts at: 6615 7431 7503 8078 8555 8669 9459 9633 9836
Size: 816 72 575 477 114 790 174 203
Cuts at: 9836 10086 10302 10902 10920 11150 11249 11991 12076
Size: 250 216 600 18 230 99 742 85
Cuts at: 12076 13147 1391
Size: 1071 2174
Fragments arranged by size:
2174 1265 1071 816 790 782 742 714
600 575 477 399 337 311 299 250
232 230 216 203 193 174 143 136
124 115 114 114 99 85 72 48
18 12
BspEI T'CCGG_A
Cuts at: 9462 13593 9462
Size: 4131 9799
BspHI T'CATG_A
Cuts at: 2718 11819 13549 2718
Size: 9101 1730 3099
Fragments arranged by size:
9101 3099 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4427 5030 6275 6897 7353 9743 10028 11143
Size: 3583 603 1245 622 456 2390 285 1115
Cuts at: 11143 11285 844
Size: 142 3489
Fragments arranged by size:
3583 3489 2390 1245 1115 622 603 456
285 142
BsrBI CCG'CTC
Cuts at: 1394 1823 11817 1394
Size: 429 9994 3507
Fragments arranged by size:
9994 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 3884 4275 5188 5874 12425 12599
Size: 1183 856 1780 391 913 686 6551 174
Cuts at: 12599 65
Size: 1396
Fragments arranged by size:
6551 1780 1396 1183 913 856 686 391
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 4298 4848 5183 12574 12761 1700
Size: 110 245 2243 550 335 7391 187 2869
Fragments arranged by size:
7391 2869 2243 550 335 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 4988 7483 7659 96
Size: 4892 2495 176 6367
Fragments arranged by size:
6367 4892 2495 176
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13930
BssSI C'ACGA_G
Cuts at: 5147 7304 11990 13002 5147
Size: 2157 4686 1012 6075
Fragments arranged by size:
6075 4686 2157 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3093 4321 5451 9202 794
Size: 2299 1228 1130 3751 5522
Fragments arranged by size:
5522 3751 2299 1228 1130
BstBI TT'CG_AA
Cuts at: 10765 11184 12734 10765
Size: 419 1550 11961
Fragments arranged by size:
11961 1550 419
BstEII G'GTnAC_C
Cuts at: 1184 3592 5179 6287 1184
Size: 2408 1587 1108 8827
Fragments arranged by size:
8827 2408 1587 1108
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 4102 8044 8200 8569 9766 2650
Size: 1452 3942 156 369 1197 6814
Fragments arranged by size:
6814 3942 1452 1197 369 156
BstYI r'GATC_y
Cuts at: 1972 2205 2713 2752 3618 11260 11627 12012 12029
Size: 233 508 39 866 7642 367 385 17
Cuts at: 12029 13481 13567 13579 13925 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
7642 1977 1452 866 508 385 367 346
233 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 5285 5285
Size: 13930
BtgI C'CryG_G
Cuts at: 513 1065 2843 3108 3675 4237 4840 4957 6556
Size: 552 1778 265 567 562 603 117 1599
Cuts at: 6556 8187 8887 9726 9934 10461 11113 513
Size: 1631 700 839 208 527 652 3330
Fragments arranged by size:
3330 1778 1631 1599 839 700 652 603
567 562 552 527 265 208 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5187 6636 487
Size: 683 56 3961 1449 7781
Fragments arranged by size:
7781 3961 1449 683 56
BtsI GCAGTG_nn'
Cuts at: 2057 2876 3391 4322 4802 6590 8514 9568 9745
Size: 819 515 931 480 1788 1924 1054 177
Cuts at: 9745 9908 10373 10671 10803 11446 12240 12260 2057
Size: 163 465 298 132 643 794 20 3727
Fragments arranged by size:
3727 1924 1788 1054 931 819 794 643
515 480 465 298 177 163 132 20
ClaI AT'CG_AT
Cuts at: 1554 9216 1554
Size: 7662 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8869 8904 529
Size: 35 8305 35 5555
Fragments arranged by size:
8305 5555 35 35
DraI TTT'AAA
Cuts at: 7035 8809 11234 7035
Size: 1774 2425 9731
Fragments arranged by size:
9731 2425 1774
DraIII CAC_nnn'GTG
Cuts at: 3160 4997 5069 6512 8651 3160
Size: 1837 72 1443 2139 8439
Fragments arranged by size:
8439 2139 1837 1443 72
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10847 12937 809
Size: 507 9531 2090 1802
Fragments arranged by size:
9531 2090 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 4110 4240
Size: 54 1425 50 275 18 994 1286 130
Cuts at: 4240 5185 5215 6975 7761 7833 8046 8190 9994
Size: 945 30 1760 786 72 213 144 1804
Cuts at: 9994 10447 11377 12266 13895 8
Size: 453 930 889 1629 43
Fragments arranged by size:
1804 1760 1629 1425 1286 994 945 930
889 786 453 275 213 144 130 72
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 7833 10447 11377 1537
Size: 275 6021 2614 930 4090
Fragments arranged by size:
6021 4090 2614 930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3894 5541 6057 7083 7438 8474 10947 11860
Size: 2541 1647 516 1026 355 1036 2473 913
Cuts at: 11860 1353
Size: 3423
Fragments arranged by size:
3423 2541 2473 1647 1036 1026 913 516
355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3771 12517 12891 13037 387
Size: 1064 138 2182 8746 374 146 1280
Fragments arranged by size:
8746 2182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7429 8667 7429
Size: 1238 12692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2962 4637 5058 5336 5510 5735 6791
Size: 393 1330 1675 421 278 174 225 1056
Cuts at: 6791 7064 7200 7834 8028 9473 10145 10231 10286
Size: 273 136 634 194 1445 672 86 55
Cuts at: 10286 10778 11155 11993 12590 13377 13645 1239
Size: 492 377 838 597 787 268 1524
Fragments arranged by size:
1675 1524 1445 1330 1056 838 787 672
634 597 492 421 393 377 278 273
268 225 194 174 136 86 55
EcoNI CCTnn'n_nnAGG
Cuts at: 4347 5318 5969 9293 9769 4347
Size: 971 651 3324 476 8508
Fragments arranged by size:
8508 3324 971 651 476
EcoO109I rG'GnC_Cy
Cuts at: 3213 3410 3411 4155 5352 5505 5596 6603 6841
Size: 197 1 744 1197 153 91 1007 238
Cuts at: 6841 8545 9119 9298 3213
Size: 1704 574 179 7845
Fragments arranged by size:
7845 1704 1197 1007 744 574 238 197
179 153 91 1
EcoRI G'AATT_C
Cuts at: 2654 7376 9885 11239 11617 2654
Size: 4722 2509 1354 378 4967
Fragments arranged by size:
4967 4722 2509 1354 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8258 8258
Size: 13930
FspI TGC'GCA
Cuts at: 4287 11096 12436 4287
Size: 6809 1340 5781
Fragments arranged by size:
6809 5781 1340
FspAI rTGC'GCAy
Cuts at: 4287 4287
Size: 13930
HaeII r_GCGC'y
Cuts at: 1678 2628 3652 4644 7304 10143 11004 13077 1678
Size: 950 1024 992 2660 2839 861 2073 2531
Fragments arranged by size:
2839 2660 2531 2073 1024 992 950 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2299 2331 2468 2500 3610
Size: 32 135 32 727 32 137 32 1110
Cuts at: 3610 3642 3866 3898 5031 5063 5149 5181 5921
Size: 32 224 32 1133 32 86 32 740
Cuts at: 5921 5953 6056 6088 6560 6592 7175 7207 8367
Size: 32 103 32 472 32 583 32 1160
Cuts at: 8367 8399 8537 8569 8656 8688 8757 8789 9015
Size: 32 138 32 87 32 69 32 226
Cuts at: 9015 9047 9050 9082 9890 9922 12571 12603 12645
Size: 32 3 32 808 32 2649 32 42
Cuts at: 12645 12677 1373
Size: 32 2626
Fragments arranged by size:
2649 2626 1160 1133 1110 808 740 727
583 472 226 224 138 137 135 103
87 86 69 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4223 5157 7600 8143 10009 10444
Size: 988 281 2285 934 2443 543 1866 435
Cuts at: 10444 10603 10864 10984 11274 11520 669
Size: 159 261 120 290 246 3079
Fragments arranged by size:
3079 2443 2285 1866 988 934 543 435
290 281 261 246 159 120
HindIII A'AGCT_T
Cuts at: 748 6857 7886 9361 748
Size: 6109 1029 1475 5317
Fragments arranged by size:
6109 5317 1475 1029
HpaI GTT'AAC
Cuts at: 10603 11520 10603
Size: 917 13013
KasI G'GCGC_C
Cuts at: 3648 4640 3648
Size: 992 12938
KpnI G_GTAC'C
Cuts at: 2929 6145 9735 11255 2929
Size: 3216 3590 1520 5604
Fragments arranged by size:
5604 3590 3216 1520
MfeI C'AATT_G
Cuts at: 5137 9797 11529 5137
Size: 4660 1732 7538
Fragments arranged by size:
7538 4660 1732
MluI A'CGCG_T
Cuts at: 13806 13806
Size: 13930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 2782 4038 4425 5774 8734 13044 13228
Size: 1326 151 1256 387 1349 2960 4310 184
Cuts at: 13228 1305
Size: 2007
Fragments arranged by size:
4310 2960 2007 1349 1326 1256 387 184
151
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2826 6977 7763 8048 8192
Size: 54 1425 343 994 4151 786 285 144
Cuts at: 8192 9996 10
Size: 1804 3944
Fragments arranged by size:
4151 3944 1804 1425 994 786 343 285
144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4242 4428 5640 5816 6020 6297 6647
Size: 648 3076 186 1212 176 204 277 350
Cuts at: 6647 6908 7152 7278 7560 7577 11888 12247 12406
Size: 261 244 126 282 17 4311 359 159
Cuts at: 12406 518
Size: 2042
Fragments arranged by size:
4311 3076 2042 1212 648 359 350 282
277 261 244 204 186 176 159 126
17
MspA1I CmG'CkG
Cuts at: 1606 3677 3979 4115 4250 4560 7222 7631 10251
Size: 2071 302 136 135 310 2662 409 2620
Cuts at: 10251 10306 10615 10889 11115 11346 12023 13171 13416
Size: 55 309 274 226 231 677 1148 245
Cuts at: 13416 1606
Size: 2120
Fragments arranged by size:
2662 2620 2120 2071 1148 677 409 310
309 302 274 245 231 226 136 135
55
NaeI GCC'GGC
Cuts at: 2057 4850 2057
Size: 2793 11137
NarI GG'CG_CC
Cuts at: 3649 4641 3649
Size: 992 12938
NcoI C'CATG_G
Cuts at: 513 1065 2843 3108 4840 4957 8187 9934 10461
Size: 552 1778 265 1732 117 3230 1747 527
Cuts at: 10461 513
Size: 3982
Fragments arranged by size:
3982 3230 1778 1747 1732 552 527 265
117
NdeI CA'TA_TG
Cuts at: 387 2365 6766 10894 387
Size: 1978 4401 4128 3423
Fragments arranged by size:
4401 4128 3423 1978
NgoMIV G'CCGG_C
Cuts at: 2055 4848 2055
Size: 2793 11137
NheI G'CTAG_C
Cuts at: 1052 9205 11131 1052
Size: 8153 1926 3851
Fragments arranged by size:
8153 3851 1926
NotI GC'GGCC_GC
Cuts at: 11377 11377
Size: 13930
NspI r_CATG'y
Cuts at: 1132 1229 2171 2258 2812 2976 2985 3827 7090
Size: 97 942 87 554 164 9 842 3263
Cuts at: 7090 7400 7582 9677 11288 12833 1132
Size: 310 182 2095 1611 1545 2229
Fragments arranged by size:
3263 2229 2095 1611 1545 942 842 554
310 182 164 97 87 9
PasI CC'CwG_GG
Cuts at: 1348 5437 6137 8705 1348
Size: 4089 700 2568 6573
Fragments arranged by size:
6573 4089 2568 700
PciI A'CATG_T
Cuts at: 2972 7396 7578 9673 12829 2972
Size: 4424 182 2095 3156 4073
Fragments arranged by size:
4424 4073 3156 2095 182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2277 5036 5695 5840 1108
Size: 1169 2759 659 145 9198
Fragments arranged by size:
9198 2759 1169 659 145
PfoI T'CCnGG_A
Cuts at: 6807 9262 6807
Size: 2455 11475
PmeI GTTT'AAAC
Cuts at: 11234 11234
Size: 13930
PmlI CAC'GTG
Cuts at: 9628 9628
Size: 13930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10493 10525 11999 12031 13512 13544 10493
Size: 32 1474 32 1481 32 10879
Fragments arranged by size:
10879 1481 1474 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3213 4155 5505 6841 8545 9119 9298 3213
Size: 942 1350 1336 1704 574 179 7845
Fragments arranged by size:
7845 1704 1350 1336 942 574 179
PshAI GACnn'nnGTC
Cuts at: 1613 3356 6438 1613
Size: 1743 3082 9105
Fragments arranged by size:
9105 3082 1743
PsiI TTA'TAA
Cuts at: 9150 11500 9150
Size: 2350 11580
PspOMI G'GGCC_C
Cuts at: 3410 3410
Size: 13930
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 10643 10675 10643
Size: 32 13898
PstI C_TGCA'G
Cuts at: 830 2871 3542 4559 4865 5447 6215 6999 8178
Size: 2041 671 1017 306 582 768 784 1179
Cuts at: 8178 8371 9740 11282 830
Size: 193 1369 1542 3478
Fragments arranged by size:
3478 2041 1542 1369 1179 1017 784 768
671 582 306 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13930
PvuII CAG'CTG
Cuts at: 3979 4250 10306 10615 10889 3979
Size: 271 6056 309 274 7020
Fragments arranged by size:
7020 6056 309 274 271
SacI G_AGCT'C
Cuts at: 7431 8669 7431
Size: 1238 12692
SacII CC_GC'GG
Cuts at: 3678 11116 3678
Size: 7438 6492
SalI G'TCGA_C
Cuts at: 1936 5155 7598 10007 10442 11272 1936
Size: 3219 2443 2409 435 830 4594
Fragments arranged by size:
4594 3219 2443 2409 830 435
SanDI GG'GwC_CC
Cuts at: 3213 3213
Size: 13930
SbfI CC_TGCA'GG
Cuts at: 11282 11282
Size: 13930
ScaI AGT'ACT
Cuts at: 3704 12178 13890 3704
Size: 8474 1712 3744
Fragments arranged by size:
8474 3744 1712
SexAI A'CCwGG_T
Cuts at: 4101 6508 7566 7602 9161 4101
Size: 2407 1058 36 1559 8870
Fragments arranged by size:
8870 2407 1559 1058 36
SfcI C'TryA_G
Cuts at: 826 1045 2524 2867 3538 4555 4861 5443 6211
Size: 219 1479 343 671 1017 306 582 768
Cuts at: 6211 6995 7609 8174 8367 9736 11278 12413 13094
Size: 784 614 565 193 1369 1542 1135 681
Cuts at: 13094 13285 826
Size: 191 1471
Fragments arranged by size:
1542 1479 1471 1369 1135 1017 784 768
681 671 614 582 565 343 306 219
193 191
SfoI GGC'GCC
Cuts at: 3650 4642 3650
Size: 992 12938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13930
SgrAI Cr'CCGG_yG
Cuts at: 1700 4298 1700
Size: 2598 11332
SmaI CCC'GGG
Cuts at: 1872 3497 4467 10088 11257 1872
Size: 1625 970 5621 1169 4545
Fragments arranged by size:
5621 4545 1625 1169 970
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2341 2579 4751 5563 5772 6481
Size: 197 926 398 238 2172 812 209 709
Cuts at: 6481 7169 12034 12935 13197 820
Size: 688 4865 901 262 1553
Fragments arranged by size:
4865 2172 1553 926 901 812 709 688
398 262 238 209 197
SnaBI TAC'GTA
Cuts at: 493 11721 493
Size: 11228 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13930
SphI G_CATG'C
Cuts at: 1132 3827 11288 1132
Size: 2695 7461 3774
Fragments arranged by size:
7461 3774 2695
SspI AAT'ATT
Cuts at: 5 52 5891 11854 5
Size: 47 5839 5963 2081
Fragments arranged by size:
5963 5839 2081 47
StuI AGG'CCT
Cuts at: 1517 2067 3446 3725 5132 8828 8853 9699 10039
Size: 550 1379 279 1407 3696 25 846 340
Cuts at: 10039 10524 1517
Size: 485 4923
Fragments arranged by size:
4923 3696 1407 1379 846 550 485 340
279 25
StyI C'CwwG_G
Cuts at: 513 1065 2843 3108 3730 4840 4957 5238 5680
Size: 552 1778 265 622 1110 117 281 442
Cuts at: 5680 7438 8187 9323 9765 9934 10339 10461 10516
Size: 1758 749 1136 442 169 405 122 55
Cuts at: 10516 11369 513
Size: 853 3074
Fragments arranged by size:
3074 1778 1758 1136 1110 853 749 622
552 442 442 405 281 265 169 122
117 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2533 2656 3357 5591 6069 8880 9302
Size: 1255 953 123 701 2234 478 2811 422
Cuts at: 9302 9520 10580 11033 11237 11957 11974 12127 12312
Size: 218 1060 453 204 720 17 153 185
Cuts at: 12312 12757 325
Size: 445 1498
Fragments arranged by size:
2811 2234 1498 1255 1060 953 720 701
478 453 445 422 218 204 185 153
123 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3702 4988 5718
Size: 274 80 33 51 1443 1725 1286 730
Cuts at: 5718 5958 6305 7483 7659 12176 13888 96
Size: 240 347 1178 176 4517 1712 138
Fragments arranged by size:
4517 1725 1712 1443 1286 1178 730 347
274 240 176 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3433 3465 4147 4179 4428 4460 4927 4959 5108
Size: 32 682 32 249 32 467 32 149
Cuts at: 5108 5140 8867 8899 11029 11061 13890 13922 3433
Size: 32 3727 32 2130 32 2829 32 3441
Fragments arranged by size:
3727 3441 2829 2130 682 467 249 149
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4598 7568 7604 11224 1316
Size: 3282 2970 36 3620 4022
Fragments arranged by size:
4022 3620 3282 2970 36
XbaI T'CTAG_A
Cuts at: 7495 11266 7495
Size: 3771 10159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4102 4430 4811 6403 9364 9528 10468 10495 10865
Size: 328 381 1592 2961 164 940 27 370
Cuts at: 10865 4102
Size: 7167
Fragments arranged by size:
7167 2961 1592 940 381 370 328 164
27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 13930
XmaI C'CCGG_G
Cuts at: 1870 3495 4465 10086 11255 1870
Size: 1625 970 5621 1169 4545
Fragments arranged by size:
5621 4545 1625 1169 970
XmnI GAAnn'nnTTC
Cuts at: 12059 12059
Size: 13930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3832 5295 9906 11738
Size: 53 83 186 936 2298 1463 4611 1832
Cuts at: 11738 276
Size: 2468
Fragments arranged by size:
4611 2468 2298 1832 1463 936 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI
NsiI PacI PspXI RsrII SapI SfiI SrfI SwaI