Restriction map for
pFN21AE2419
(Circular) (Six-base) MAPSORT of: pFN21AE2419.seq Check: 7076 from: 1 to: 6775
pFN21AE2419 6775 bp
With 182 enzymes: SgfI *
August 14, 2012 13:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2602
Size: 6775
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4118 1937
Size: 2181 4594
Acc65I G'GTAC_C
Cuts at: 3510 4096 3510
Size: 586 6189
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2389 3824 4838 6222 6490 1632
Size: 581 176 1435 1014 1384 268 1917
Fragments arranged by size:
1917 1435 1384 1014 581 268 176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 3745 5674 6651 1203
Size: 2542 1929 977 1327
Fragments arranged by size:
2542 1929 1327 977
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 2198 1166
Size: 1032 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2100 3122 1184
Size: 916 1022 4837
Fragments arranged by size:
4837 1022 916
AlwNI CAG_nnn'CTG
Cuts at: 4518 6090 4518
Size: 1572 5203
ApaLI G'TGCA_C
Cuts at: 4832 5988 4832
Size: 1156 5619
ApoI r'AATT_y
Cuts at: 1493 3070 4084 4310 4462 6571 1493
Size: 1577 1014 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 1014 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5329 160
Size: 872 4297 1606
Fragments arranged by size:
4297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2726 4100 1870
Size: 73 293 490 1374 4545
Fragments arranged by size:
4545 1374 490 293 73
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 3098 3395 3510 4096
Size: 325 217 627 344 967 297 115 586
Cuts at: 4096 5551 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 967 627 586 344 325 297
217 115
BanII G_rGCy'C
Cuts at: 1391 2193 2483 3170 3386 4094 1391
Size: 802 290 687 216 708 4072
Fragments arranged by size:
4072 802 708 687 290 216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3212 3586 3685 928
Size: 971 1313 374 99 4018
Fragments arranged by size:
4018 1313 971 374 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5877 4667
Size: 1210 5565
BclI T'GATC_A
Cuts at: 1548 3144 3603 1548
Size: 1596 459 4720
Fragments arranged by size:
4720 1596 459
BfrBI ATG'CAT
Cuts at: 3443 3443
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3416 5386 136
Size: 107 122 71 631 605 1744 1970 1525
Fragments arranged by size:
1970 1744 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2788 6770 2788
Size: 3982 2793
BlpI GC'TnA_GC
Cuts at: 4192 4192
Size: 6775
Bme1580I G_kGCm'C
Cuts at: 1790 3101 4836 5992 1790
Size: 1311 1735 1156 2573
Fragments arranged by size:
2573 1735 1311 1156
BmgBI CAC'GTC
Cuts at: 1318 3315 1318
Size: 1997 4778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2467 3443 3749 5435 1239
Size: 1228 976 306 1686 2579
Fragments arranged by size:
2579 1686 1228 976 306
Bpu10I CC'TnA_GC
Cuts at: 3424 4026 3424
Size: 602 6173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3161 4900 5765 6063 3161
Size: 1739 865 298 3873
Fragments arranged by size:
3873 1739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 3986 5438 882
Size: 617 600 1887 1452 2219
Fragments arranged by size:
2219 1887 1452 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 4566 493
Size: 711 3362 2702
Fragments arranged by size:
3362 2702 711
BsaBI GATnn'nnATC
Cuts at: 3338 6769 3338
Size: 3431 3344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5208 5606 5880 6027 6438 5208
Size: 398 274 147 411 5545
Fragments arranged by size:
5545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2643 2673 2957 2987 3702
Size: 30 165 30 1240 30 284 30 715
Cuts at: 3702 3732 1178
Size: 30 4221
Fragments arranged by size:
4221 1240 715 284 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3463 3965 1186
Size: 2277 502 3996
Fragments arranged by size:
3996 2277 502
BseYI C'CCAG_C
Cuts at: 2034 2739 3379 3556 5978 6474 2034
Size: 705 640 177 2422 496 2335
Fragments arranged by size:
2422 2335 705 640 496 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 2953 1163
Size: 573 759 458 4985
Fragments arranged by size:
4985 759 573 458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 2483 2697 3177 4836 4921 5992 2345
Size: 138 214 480 1659 85 1071 3128
Fragments arranged by size:
3128 1659 1071 480 214 138 85
BsmI GAATG_Cn'
Cuts at: 4067 4286 4379 4067
Size: 219 93 6463
Fragments arranged by size:
6463 219 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3829 3829
Size: 6775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2345 2483 2697 3101 3170 3177
Size: 399 403 152 138 214 404 69 7
Cuts at: 3177 3386 4094 4836 4921 5992 1391
Size: 209 708 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 708 404 403 399 214
209 152 138 85 69 7
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 3147 4664 6394 3147
Size: 1517 1730 3528
Fragments arranged by size:
3528 1730 1517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2060 2602 3175 4130 844
Size: 1216 542 573 955 3489
Fragments arranged by size:
3489 1216 955 573 542
BsrBI CCG'CTC
Cuts at: 1394 1823 4662 1394
Size: 429 2839 3507
Fragments arranged by size:
3507 2839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2525 2871 3445 5270 5444 65
Size: 1183 1277 346 574 1825 174 1396
Fragments arranged by size:
1825 1396 1277 1183 574 346 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3653 3836 5419 5606 1700
Size: 110 1843 183 1583 187 2869
Fragments arranged by size:
2869 1843 1583 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6775
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 4835 5847 4835
Size: 1012 5763
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3933 3936 794
Size: 3139 3 3633
Fragments arranged by size:
3633 3139 3
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 2461 1184
Size: 1277 5498
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2297 2378 2560 3544 3556 2200
Size: 97 81 182 984 12 5419
Fragments arranged by size:
5419 984 182 97 81 12
BstYI r'GATC_y
Cuts at: 1972 2788 3085 3159 4105 4472 4857 4874 6326
Size: 816 297 74 946 367 385 17 1452
Cuts at: 6326 6412 6424 6770 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 946 816 385 367 346 297
86 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2703 513
Size: 552 939 165 534 4585
Fragments arranged by size:
4585 939 552 534 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3028 487
Size: 683 56 1802 4234
Fragments arranged by size:
4234 1802 683 56
BtsI GCAGTG_nn'
Cuts at: 2082 4291 5085 5105 2082
Size: 2209 794 20 3752
Fragments arranged by size:
3752 2209 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 4079 4079
Size: 6775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 809
Size: 507 4466 1802
Fragments arranged by size:
4466 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 3258 3417
Size: 54 1425 50 275 18 882 546 159
Cuts at: 3417 3546 3651 4222 5111 6740 8
Size: 129 105 571 889 1629 43
Fragments arranged by size:
1629 1425 889 882 571 546 275 159
129 105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 2527 2632 4705 1353
Size: 879 295 105 2073 3423
Fragments arranged by size:
3423 2073 879 295 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5362 5736 5882 387
Size: 1064 138 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2481 2481
Size: 6775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2389 2467 3443 3749 3824 4838
Size: 393 581 176 78 976 306 75 1014
Cuts at: 4838 5435 6222 6490 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1014 976 787 597 581 393 306
268 176 78 75
EcoO109I rG'GnC_Cy
Cuts at: 4036 4036
Size: 6775
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 2367 5922 1678
Size: 689 3555 2531
Fragments arranged by size:
3555 2531 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2643 2675 3352 3384 5416
Size: 32 135 32 1071 32 677 32 2032
Cuts at: 5416 5448 5490 5522 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2032 1071 677 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 4119 4365 669
Size: 988 281 1308 873 246 3079
Fragments arranged by size:
3079 1308 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 3514 4100 3514
Size: 586 6189
MfeI C'AATT_G
Cuts at: 4374 4374
Size: 6775
MluI A'CGCG_T
Cuts at: 3745 6651 3745
Size: 2906 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2359 2451 2969 3411 3745 5889 6073 1305
Size: 1054 92 518 442 334 2144 184 2007
Fragments arranged by size:
2144 2007 1054 518 442 334 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 3260 3548 10
Size: 54 1425 343 882 546 288 3237
Fragments arranged by size:
3237 1425 882 546 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2558 2612 3542 4733 5092 5251
Size: 648 1032 360 54 930 1191 359 159
Cuts at: 5251 518
Size: 2042
Fragments arranged by size:
2042 1191 1032 930 648 360 359 159
54
MspA1I CmG'CkG
Cuts at: 1606 2374 2836 3628 3787 3924 4191 4868 6016
Size: 768 462 792 159 137 267 677 1148
Cuts at: 6016 6261 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 792 768 677 462 267 245
159 137
NaeI GCC'GGC
Cuts at: 3655 3655
Size: 6775
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5284
Fragments arranged by size:
5284 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NgoMIV G'CCGG_C
Cuts at: 3653 3653
Size: 6775
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NsiI A_TGCA'T
Cuts at: 3445 3445
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 4133 5678 1132
Size: 97 2904 1545 2229
Fragments arranged by size:
2904 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3810 1348
Size: 2462 4313
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6775
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 6775
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4036 4036
Size: 6775
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 4345 4345
Size: 6775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 6743
PstI C_TGCA'G
Cuts at: 830 3732 3929 4127 830
Size: 2902 197 198 3478
Fragments arranged by size:
3478 2902 198 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
PvuII CAG'CTG
Cuts at: 2374 2836 2374
Size: 462 6313
SacI G_AGCT'C
Cuts at: 2483 2483
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 2632 2632
Size: 6775
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SfcI C'TryA_G
Cuts at: 826 1045 3728 3925 4123 5258 5939 6130 826
Size: 219 2683 197 198 1135 681 191 1471
Fragments arranged by size:
2683 1471 1135 681 219 198 197 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3176 4879 5780 6042 820
Size: 197 926 783 450 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 783 450 262 197
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 4133 1132
Size: 3001 3774
SspI AAT'ATT
Cuts at: 5 52 4699 5
Size: 47 4647 2081
Fragments arranged by size:
4647 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2004 2290 3549 4071 4214 513
Size: 552 939 286 1259 522 143 3074
Fragments arranged by size:
3074 1259 939 552 522 286 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2732 4073 4802 4819 4972 5157 5602
Size: 1255 1152 1341 729 17 153 185 445
Cuts at: 5602 325
Size: 1498
Fragments arranged by size:
1498 1341 1255 1152 729 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5021 6733 96
Size: 274 80 33 51 1443 3044 1712 138
Fragments arranged by size:
3044 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3330 3362 6735 6767 3330
Size: 32 3373 32 3338
Fragments arranged by size:
3373 3338 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 4111 4111
Size: 6775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2768 3412 2434
Size: 334 644 5797
Fragments arranged by size:
5797 644 334
XhoI C'TCGA_G
Cuts at: 1943 2726 1943
Size: 783 5992
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 4904 4904
Size: 6775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI PacI PmlI PspOMI
PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI