Restriction map for
pFN21AE2418
(Circular) (Six-base) MAPSORT of: pFN21AE2418.seq Check: 1908 from: 1 to: 7222
pFN21AE2418 7222 bp
With 182 enzymes: SgfI *
October 12, 2018 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2072 3182 3914 4177 2072
Size: 1110 732 263 5117
Fragments arranged by size:
5117 1110 732 263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2688 5032 278
Size: 53 83 186 936 1152 2344 2468
Fragments arranged by size:
2468 2344 1152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3327 3462 3753 4565 1937
Size: 1390 135 291 812 4594
Fragments arranged by size:
4594 1390 812 291 135
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 5349 5722 1563
Size: 3786 373 3063
Fragments arranged by size:
3786 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 2996 3580 4216 4490 5285 6669 6937
Size: 953 411 584 636 274 795 1384 268
Cuts at: 6937 1632
Size: 1917
Fragments arranged by size:
1917 1384 953 795 636 584 411 274
268
AflII C'TTAA_G
Cuts at: 820 1017 4145 820
Size: 197 3128 3897
Fragments arranged by size:
3897 3128 197
AflIII A'CryG_T
Cuts at: 1203 6121 7098 1203
Size: 4918 977 1327
Fragments arranged by size:
4918 1327 977
AgeI A'CCGG_T
Cuts at: 2279 4221 6053 2279
Size: 1942 1832 3448
Fragments arranged by size:
3448 1942 1832
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 3584 3785 4271 1166
Size: 2418 201 486 4117
Fragments arranged by size:
4117 2418 486 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2882 4199 1184
Size: 1698 1317 4207
Fragments arranged by size:
4207 1698 1317
AlwNI CAG_nnn'CTG
Cuts at: 2392 3565 4365 4965 6537 2392
Size: 1173 800 600 1572 3077
Fragments arranged by size:
3077 1572 1173 800 600
ApaI G_GGCC'C
Cuts at: 3135 3135
Size: 7222
ApaLI G'TGCA_C
Cuts at: 2776 3137 5279 6435 2776
Size: 361 2142 1156 3563
Fragments arranged by size:
3563 2142 1156 361
ApoI r'AATT_y
Cuts at: 1493 4531 4757 4909 7018 1493
Size: 3038 226 152 2109 1697
Fragments arranged by size:
3038 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 4294 4410 4433 4547 1870
Size: 73 1143 1208 116 23 114 4545
Fragments arranged by size:
4545 1208 1143 116 114 73 23
BamHI G'GATC_C
Cuts at: 4552 4919 4552
Size: 367 6855
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 3096 4543 5998 618
Size: 325 217 627 495 814 1447 1455 1842
Fragments arranged by size:
1842 1455 1447 814 627 495 325 217
BanII G_rGCy'C
Cuts at: 1391 2991 3087 3135 4541 1391
Size: 1600 96 48 1406 4072
Fragments arranged by size:
4072 1600 1406 96 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2333 2477 3521 3754 3810 3973 4296
Size: 971 434 144 1044 233 56 163 323
Cuts at: 4296 4483 928
Size: 187 3667
Fragments arranged by size:
3667 1044 971 434 323 233 187 163
144 56
BbvCI CC'TCA_GC
Cuts at: 4485 4485
Size: 7222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5114 6324 5114
Size: 1210 6012
BclI T'GATC_A
Cuts at: 1548 3342 1548
Size: 1794 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5833 136
Size: 107 122 71 631 605 4161 1525
Fragments arranged by size:
4161 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7217 7217
Size: 7222
BlpI GC'TnA_GC
Cuts at: 4428 4639 4428
Size: 211 7011
Bme1580I G_kGCm'C
Cuts at: 1790 2780 3135 3141 5283 6439 1790
Size: 990 355 6 2142 1156 2573
Fragments arranged by size:
2573 2142 1156 990 355 6
BmgBI CAC'GTC
Cuts at: 1318 3840 1318
Size: 2522 4700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4953 5911 442
Size: 416 53 83 399 24 3536 958 1753
Fragments arranged by size:
3536 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 3202 2233
Size: 969 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2091 2414 2558 3002 3050 3215 3453
Size: 841 11 323 144 444 48 165 238
Cuts at: 3453 3932 3968 5882 1239
Size: 479 36 1914 2579
Fragments arranged by size:
2579 1914 841 479 444 323 238 165
144 48 36 11
Bpu10I CC'TnA_GC
Cuts at: 4171 4325 4485 4171
Size: 154 160 6908
Fragments arranged by size:
6908 160 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3590 5347 6212 6510 3590
Size: 1757 865 298 4302
Fragments arranged by size:
4302 1757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 3215 3723 4470 5885 882
Size: 617 580 1136 508 747 1415 2219
Fragments arranged by size:
2219 1415 1136 747 617 580 508
BsaAI yAC'GTr
Cuts at: 493 1204 5013 493
Size: 711 3809 2702
Fragments arranged by size:
3809 2702 711
BsaBI GATnn'nnATC
Cuts at: 2945 3692 7216 2945
Size: 747 3524 2951
Fragments arranged by size:
3524 2951 747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 3011 4311 5029
Size: 53 83 186 936 1152 326 1300 718
Cuts at: 5029 5411 6046 275
Size: 382 635 1451
Fragments arranged by size:
1451 1300 1152 936 718 635 382 326
186 83 53
BsaWI w'CCGG_w
Cuts at: 2279 4221 5655 6053 6327 6474 6885 2279
Size: 1942 1434 398 274 147 411 2616
Fragments arranged by size:
2616 1942 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2670 2700 3074
Size: 30 165 30 675 30 562 30 374
Cuts at: 3074 3104 1178
Size: 30 5296
Fragments arranged by size:
5296 675 562 374 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2999 3133 3471 1186
Size: 1000 813 134 338 4937
Fragments arranged by size:
4937 1000 813 338 134
BseYI C'CCAG_C
Cuts at: 2118 2788 3029 6425 6921 2118
Size: 670 241 3396 496 2419
Fragments arranged by size:
3396 2419 670 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2606 2855 2948 3112 1163
Size: 573 870 249 93 164 5273
Fragments arranged by size:
5273 870 573 249 164 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2832 2904 3506 4672 5433 6461
Size: 275 186 831 72 602 1166 761 1028
Cuts at: 6461 1540
Size: 2301
Fragments arranged by size:
2301 1166 1028 831 761 602 275 186
72
BsiHKAI G_wGCw'C
Cuts at: 2780 2991 3141 4411 5283 5368 6439 2780
Size: 211 150 1270 872 85 1071 3563
Fragments arranged by size:
3563 1270 1071 872 211 150 85
BsmI GAATG_Cn'
Cuts at: 4733 4826 4733
Size: 93 7129
BsmBI CGTCTCn'nnnn_
Cuts at: 2262 2632 2262
Size: 370 6852
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2780 2991 3087 3135 3141 4411 4541
Size: 399 990 211 96 48 6 1270 130
Cuts at: 4541 5283 5368 6439 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1270 1071 990 742 399 211 130
96 85 48 6
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 3705 5111 6841 3705
Size: 1406 1730 4086
Fragments arranged by size:
4086 1730 1406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2508 3182 3914 4177 4577 844
Size: 1228 436 674 732 263 400 3489
Fragments arranged by size:
3489 1228 732 674 436 400 263
BsrBI CCG'CTC
Cuts at: 1394 1823 4170 5109 1394
Size: 429 2347 939 3507
Fragments arranged by size:
3507 2347 939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5717 5891 65
Size: 1183 4469 174 1396
Fragments arranged by size:
4469 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 4221 5866 6053 1700
Size: 110 469 1942 1645 187 2869
Fragments arranged by size:
2869 1942 1645 469 187 110
BsrGI T'GTAC_A
Cuts at: 96 2931 3336 96
Size: 2835 405 3982
Fragments arranged by size:
3982 2835 405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 2198 5282 6294 2198
Size: 3084 1012 3126
Fragments arranged by size:
3126 3084 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7222
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 2656 1184
Size: 1472 5750
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 3787 3910 3937 2788
Size: 999 123 27 6073
Fragments arranged by size:
6073 999 123 27
BstYI r'GATC_y
Cuts at: 1972 2389 3075 3438 4552 4919 5304 5321 6773
Size: 417 686 363 1114 367 385 17 1452
Cuts at: 6773 6859 6871 7217 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1114 686 417 385 367 363
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2333 2333
Size: 7222
BtgI C'CryG_G
Cuts at: 513 1065 2127 2701 3354 3375 3582 3624 3673
Size: 552 1062 574 653 21 207 42 49
Cuts at: 3673 513
Size: 4062
Fragments arranged by size:
4062 1062 653 574 552 207 49 42
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2611 4027 487
Size: 683 56 1385 1416 3682
Fragments arranged by size:
3682 1416 1385 683 56
BtsI GCAGTG_nn'
Cuts at: 2618 4738 5532 5552 2618
Size: 2120 794 20 4288
Fragments arranged by size:
4288 2120 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7187
DraI TTT'AAA
Cuts at: 4526 4526
Size: 7222
DraIII CAC_nnn'GTG
Cuts at: 3120 3120
Size: 7222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3293 4487 6229 809
Size: 507 1977 1194 1742 1802
Fragments arranged by size:
1977 1802 1742 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2769 2829
Size: 54 1425 50 275 18 300 639 60
Cuts at: 2829 2889 2901 3503 3576 4669 5558 7187 8
Size: 60 12 602 73 1093 889 1629 43
Fragments arranged by size:
1629 1425 1093 889 639 602 300 275
73 60 60 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2829 2901 3503 4669 1537
Size: 275 1017 72 602 1166 4090
Fragments arranged by size:
4090 1166 1017 602 275 72
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 4130 5152 1353
Size: 1061 1716 1022 3423
Fragments arranged by size:
3423 1716 1061 1022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5809 6183 6329 387
Size: 1064 138 4220 374 146 1280
Fragments arranged by size:
4220 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2989 2989
Size: 7222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2091 2414 2558 2585 2996 3002
Size: 393 448 11 323 144 27 411 6
Cuts at: 3002 3050 3215 3453 3580 3932 3968 4216 4490
Size: 48 165 238 127 352 36 248 274
Cuts at: 4490 5285 5882 6669 6937 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 448 411 393 352
323 274 268 248 238 165 144 127
48 36 27 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2463 2882 3367 2463
Size: 419 485 6318
Fragments arranged by size:
6318 485 419
EcoO109I rG'GnC_Cy
Cuts at: 3857 3870 3857
Size: 13 7209
EcoRI G'AATT_C
Cuts at: 4531 4909 4531
Size: 378 6844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3322 3997 3322
Size: 675 6547
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 2844 6369 1678
Size: 1166 3525 2531
Fragments arranged by size:
3525 2531 1166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2670 2702 3214
Size: 32 135 32 506 32 560 32 512
Cuts at: 3214 3246 3428 3460 3824 3856 4406 4438 5863
Size: 32 182 32 364 32 550 32 1425
Cuts at: 5863 5895 5937 5969 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 560 550 512 506 364 182
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 4066 4566 4812 669
Size: 988 281 640 1488 500 246 3079
Fragments arranged by size:
3079 1488 988 640 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7222
HpaI GTT'AAC
Cuts at: 4812 4812
Size: 7222
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 4821 4821
Size: 7222
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6336 6520 1305
Size: 5031 184 2007
Fragments arranged by size:
5031 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3578 10
Size: 54 1425 343 1746 3654
Fragments arranged by size:
3654 1746 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3584 3785 3935 4271 5180 5539 5698
Size: 648 2418 201 150 336 909 359 159
Cuts at: 5698 518
Size: 2042
Fragments arranged by size:
2418 2042 909 648 359 336 201 159
150
MspA1I CmG'CkG
Cuts at: 1606 2039 2788 3016 3029 3070 3259 3675 3785
Size: 433 749 228 13 41 189 416 110
Cuts at: 3785 4638 5315 6463 6708 1606
Size: 853 677 1148 245 2120
Fragments arranged by size:
2120 1148 853 749 677 433 416 245
228 189 110 41 13
NcoI C'CATG_G
Cuts at: 513 1065 2701 3354 3624 513
Size: 552 1636 653 270 4111
Fragments arranged by size:
4111 1636 653 552 270
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 2932 3244 3661 4039 4580 6125 1132
Size: 97 1703 312 417 378 541 1545 2229
Fragments arranged by size:
2229 1703 1545 541 417 378 312 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7222
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 3688 1108
Size: 1284 1296 4642
Fragments arranged by size:
4642 1296 1284
PfoI T'CCnGG_A
Cuts at: 2535 3078 3104 4122 2535
Size: 543 26 1018 5635
Fragments arranged by size:
5635 1018 543 26
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5291 5323 6804 6836 5291
Size: 32 1481 32 5677
Fragments arranged by size:
5677 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 4792 4792
Size: 7222
PspOMI G'GGCC_C
Cuts at: 3131 3131
Size: 7222
PstI C_TGCA'G
Cuts at: 830 2418 2814 3276 3498 3561 3869 4107 4188
Size: 1588 396 462 222 63 308 238 81
Cuts at: 4188 4574 830
Size: 386 3478
Fragments arranged by size:
3478 1588 462 396 386 308 238 222
81 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
PvuII CAG'CTG
Cuts at: 2039 2788 3029 2039
Size: 749 241 6232
Fragments arranged by size:
6232 749 241
SacI G_AGCT'C
Cuts at: 2991 2991
Size: 7222
SacII CC_GC'GG
Cuts at: 3676 3676
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SapI GCTCTTCn'nnn_
Cuts at: 2414 4130 2414
Size: 1716 5506
SbfI CC_TGCA'GG
Cuts at: 3276 3869 4574 3276
Size: 593 705 5924
Fragments arranged by size:
5924 705 593
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SfcI C'TryA_G
Cuts at: 826 1045 2414 2810 3208 3272 3367 3494 3535
Size: 219 1369 396 398 64 95 127 41
Cuts at: 3535 3557 3865 4103 4184 4570 5705 6386 6577
Size: 22 308 238 81 386 1135 681 191
Cuts at: 6577 826
Size: 1471
Fragments arranged by size:
1471 1369 1135 681 398 396 386 308
238 219 191 127 95 81 64 41
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 2279 1700
Size: 579 6643
SmaI CCC'GGG
Cuts at: 1872 4296 4549 1872
Size: 2424 253 4545
Fragments arranged by size:
4545 2424 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3605 4145 4410 5326 6227 6489
Size: 197 926 1662 540 265 916 901 262
Cuts at: 6489 820
Size: 1553
Fragments arranged by size:
1662 1553 926 916 901 540 265 262
197
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 4580 1132
Size: 3448 3774
SspI AAT'ATT
Cuts at: 5 52 5146 5
Size: 47 5094 2081
Fragments arranged by size:
5094 2081 47
StuI AGG'CCT
Cuts at: 1517 4163 1517
Size: 2646 4576
StyI C'CwwG_G
Cuts at: 513 1065 2701 3354 3624 3642 4661 513
Size: 552 1636 653 270 18 1019 3074
Fragments arranged by size:
3074 1636 1019 653 552 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4043 5249 5266 5419 5604 6049 325
Size: 1255 2463 1206 17 153 185 445 1498
Fragments arranged by size:
2463 1498 1255 1206 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 3336 3591
Size: 274 80 33 51 1443 954 405 255
Cuts at: 3591 3897 5468 7180 96
Size: 306 1571 1712 138
Fragments arranged by size:
1712 1571 1443 954 405 306 274 255
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 7182 7214 3074
Size: 32 4076 32 3082
Fragments arranged by size:
4076 3082 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2306 1316
Size: 990 6232
XbaI T'CTAG_A
Cuts at: 4558 4558
Size: 7222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 4120 2851
Size: 1269 5953
XhoI C'TCGA_G
Cuts at: 1943 4410 1943
Size: 2467 4755
XmaI C'CCGG_G
Cuts at: 1870 4294 4547 1870
Size: 2424 253 4545
Fragments arranged by size:
4545 2424 253
XmnI GAAnn'nnTTC
Cuts at: 4139 4163 5351 4139
Size: 24 1188 6010
Fragments arranged by size:
6010 1188 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2686 5030 276
Size: 53 83 186 936 1152 2344 2468
Fragments arranged by size:
2468 2344 1152 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI