Restriction map for
pFN21AE2417
(Circular) (Six-base) MAPSORT of: pFN21AE2417.seq Check: 7264 from: 1 to: 6046
pFN21AE2417 6046 bp
With 182 enzymes: SgfI *
July 23, 2012 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2491 2491
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 3093 4109 5493 5761 1632
Size: 788 673 1016 1384 268 1917
Fragments arranged by size:
1917 1384 1016 788 673 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 3218 1166
Size: 2052 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2936 3149 3152 3789 5361 2936
Size: 213 3 637 1572 3621
Fragments arranged by size:
3621 1572 637 213 3
ApaLI G'TGCA_C
Cuts at: 2970 4103 5259 2970
Size: 1133 1156 3757
Fragments arranged by size:
3757 1156 1133
ApoI r'AATT_y
Cuts at: 1493 2918 3355 3581 3733 5842 1493
Size: 1425 437 226 152 2109 1697
Fragments arranged by size:
2109 1697 1425 437 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 3371 1870
Size: 73 1365 63 4545
Fragments arranged by size:
4545 1365 73 63
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2506 3367 4822 618
Size: 325 217 627 719 861 1455 1842
Fragments arranged by size:
1842 1455 861 719 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2538 3063 3107 3309 3345 3365 1391
Size: 1147 525 44 202 36 20 4072
Fragments arranged by size:
4072 1147 525 202 44 36 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2820 928
Size: 971 921 4154
Fragments arranged by size:
4154 971 921
BbvCI CC'TCA_GC
Cuts at: 2097 2097
Size: 6046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3241 3938 5148 3241
Size: 697 1210 4139
Fragments arranged by size:
4139 1210 697
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BfrBI ATG'CAT
Cuts at: 3319 3319
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2974 4107 5263 1790
Size: 1184 1133 1156 2573
Fragments arranged by size:
2573 1184 1156 1133
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2924 3777 4735
Size: 416 53 83 399 24 1507 853 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1507 958 853 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 4706 1239
Size: 1130 2337 2579
Fragments arranged by size:
2579 2337 1130
Bpu10I CC'TnA_GC
Cuts at: 2097 2532 2097
Size: 435 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 4171 5036 5334 2156
Size: 2015 865 298 2868
Fragments arranged by size:
2868 2015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 4709 882
Size: 617 744 2466 2219
Fragments arranged by size:
2466 2219 744 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 3837 493
Size: 711 1530 1103 2702
Fragments arranged by size:
2702 1530 1103 711
BsaBI GATnn'nnATC
Cuts at: 2051 6040 2051
Size: 3989 2057
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3853 4235 4870 275
Size: 53 83 186 936 2320 382 635 1451
Fragments arranged by size:
2320 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 1178
Size: 30 165 30 964 30 4827
Fragments arranged by size:
4827 964 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 5001
BseYI C'CCAG_C
Cuts at: 2890 3019 3131 3142 5249 5745 2890
Size: 129 112 11 2107 496 3191
Fragments arranged by size:
3191 2107 496 129 112 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 2974 4107 4192 5263 2538
Size: 436 1133 85 1071 3321
Fragments arranged by size:
3321 1133 1071 436 85
BsmI GAATG_Cn'
Cuts at: 2764 2883 3557 3650 2764
Size: 119 674 93 5160
Fragments arranged by size:
5160 674 119 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2538 2974 3063 3107 3309 3345 3365
Size: 399 748 436 89 44 202 36 20
Cuts at: 3365 4107 4192 5263 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 748 742 436 399 202 89
85 44 36 20
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 2781 3935 5665 2781
Size: 1154 1730 3162
Fragments arranged by size:
3162 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2491 3052 3401 844
Size: 1647 561 349 3489
Fragments arranged by size:
3489 1647 561 349
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 3220 3220
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 3376 3743 4128 4145 5597 5683 5695 6041
Size: 1404 367 385 17 1452 86 12 346
Cuts at: 6041 1972
Size: 1977
Fragments arranged by size:
1977 1452 1404 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3075 3075
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2264 3219 3243 513
Size: 552 1199 955 24 3316
Fragments arranged by size:
3316 1199 955 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 3562 4356 4376 2088
Size: 1474 794 20 3758
Fragments arranged by size:
3758 1474 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3493 4382 6011
Size: 54 1425 50 275 18 1663 889 1629
Cuts at: 6011 8
Size: 43
Fragments arranged by size:
1663 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 3976 1353
Size: 912 1711 3423
Fragments arranged by size:
3423 1711 912
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 2536
Size: 6046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2420 3093 4109 4706 5493 5761
Size: 393 737 51 673 1016 597 787 268
Cuts at: 5761 1239
Size: 1524
Fragments arranged by size:
1524 1016 787 737 673 597 393 268
51
EcoO109I rG'GnC_Cy
Cuts at: 2041 2948 3192 2041
Size: 907 244 4895
Fragments arranged by size:
4895 907 244
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2450 3123 2450
Size: 673 5373
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 2763 2795 4687
Size: 32 135 32 649 32 510 32 1892
Cuts at: 4687 4719 4761 4793 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1892 649 510 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3390 3636 669
Size: 988 281 1452 246 3079
Fragments arranged by size:
3079 1452 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 2753 3645 2753
Size: 892 5154
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4224
Fragments arranged by size:
4224 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3218 4004 4363 4522 518
Size: 648 2052 786 359 159 2042
Fragments arranged by size:
2052 2042 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3149 3462 4139 5287 5532 1606
Size: 1543 313 677 1148 245 2120
Fragments arranged by size:
2120 1543 1148 677 313 245
NcoI C'CATG_G
Cuts at: 513 1065 2264 3243 513
Size: 552 1199 979 3316
Fragments arranged by size:
3316 1199 979 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NsiI A_TGCA'T
Cuts at: 3321 3321
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6046
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2849 1108
Size: 1741 4305
PfoI T'CCnGG_A
Cuts at: 2064 2719 2842 3228 2064
Size: 655 123 386 4882
Fragments arranged by size:
4882 655 386 123
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 3192 2041
Size: 1151 4895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2166 3398 830
Size: 1336 1232 3478
Fragments arranged by size:
3478 1336 1232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 3149 3149
Size: 6046
SacI G_AGCT'C
Cuts at: 2538 2538
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2162 3394 4529 5210 5401 826
Size: 219 1117 1232 1135 681 191 1471
Fragments arranged by size:
1471 1232 1135 1117 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 4150 5051 5313 820
Size: 197 926 192 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 2306 3970 5
Size: 47 2254 1664 2081
Fragments arranged by size:
2254 2081 1664 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6046
StyI C'CwwG_G
Cuts at: 513 1065 2264 3098 3243 3485 513
Size: 552 1199 834 145 242 3074
Fragments arranged by size:
3074 1199 834 552 242 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 2192 3010 3382 2192
Size: 818 372 4856
Fragments arranged by size:
4856 818 372
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 2433 4175 2433
Size: 1742 4304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI