Restriction map for
pFN21AE2416
(Circular) (Six-base) MAPSORT of: pFN21AE2416.seq Check: 9386 from: 1 to: 6622
pFN21AE2416 6622 bp
With 182 enzymes: SgfI *
July 23, 2012 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2195 3104 3717 2195
Size: 909 613 5100
Fragments arranged by size:
5100 909 613
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3965 1937
Size: 2028 4594
Acc65I G'GTAC_C
Cuts at: 3468 3943 3468
Size: 475 6147
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 4685 6069 6337 1632
Size: 2150 903 1384 268 1917
Fragments arranged by size:
2150 1917 1384 903 268
AfeI AGC'GCT
Cuts at: 3412 3412
Size: 6622
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 5521 6498 1203
Size: 4318 977 1327
Fragments arranged by size:
4318 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 2606 1166
Size: 1440 5182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3506 1184
Size: 2322 4300
AlwNI CAG_nnn'CTG
Cuts at: 4365 5937 4365
Size: 1572 5050
ApaI G_GGCC'C
Cuts at: 2165 3655 2165
Size: 1490 5132
ApaLI G'TGCA_C
Cuts at: 3091 4679 5835 3091
Size: 1588 1156 3878
Fragments arranged by size:
3878 1588 1156
ApoI r'AATT_y
Cuts at: 1493 3931 4157 4309 6418 1493
Size: 2438 226 152 2109 1697
Fragments arranged by size:
2438 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 3451 3701 3947 1870
Size: 73 185 1323 250 246 4545
Fragments arranged by size:
4545 1323 250 246 185 73
BamHI G'GATC_C
Cuts at: 2124 2463 3074 3952 4319 2124
Size: 339 611 878 367 4427
Fragments arranged by size:
4427 878 611 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2314 2585 2919 3230
Size: 325 217 627 510 17 271 334 311
Cuts at: 3230 3353 3468 3475 3705 3943 5398 618
Size: 123 115 7 230 238 1455 1842
Fragments arranged by size:
1842 1455 627 510 334 325 311 271
238 230 217 123 115 17 7
BanII G_rGCy'C
Cuts at: 1391 2165 2492 2552 2576 2823 3060 3655 3819
Size: 774 327 60 24 247 237 595 164
Cuts at: 3819 3941 1391
Size: 122 4072
Fragments arranged by size:
4072 774 595 327 247 237 164 122
60 24
BbeI G_GCGC'C
Cuts at: 2301 3234 2301
Size: 933 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3838 928
Size: 971 1939 3712
Fragments arranged by size:
3712 1939 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3226 3260 3628 3662 4813 4847 1903
Size: 34 1289 34 368 34 1151 34 3678
Fragments arranged by size:
3678 1289 1151 368 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4514 5724 3467
Size: 1047 1210 4365
Fragments arranged by size:
4365 1210 1047
BclI T'GATC_A
Cuts at: 1548 3306 1548
Size: 1758 4864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 2295 3374
Size: 107 122 71 631 605 565 58 1079
Cuts at: 3374 3659 5233 136
Size: 285 1574 1525
Fragments arranged by size:
1574 1525 1079 631 605 565 285 122
107 71 58
BglII A'GATC_T
Cuts at: 2913 6617 2913
Size: 3704 2918
BlpI GC'TnA_GC
Cuts at: 2178 2366 4039 2178
Size: 188 1673 4761
Fragments arranged by size:
4761 1673 188
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2165 3095 3480 3641 3655 3708 4683
Size: 313 62 930 385 161 14 53 975
Cuts at: 4683 5839 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 975 930 385 313 161 62
53 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2048 3347 4353
Size: 416 53 83 399 24 631 1299 1006
Cuts at: 4353 5311 442
Size: 958 1753
Fragments arranged by size:
1753 1299 1006 958 631 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2989 3838 2566
Size: 423 849 5350
Fragments arranged by size:
5350 849 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2264 2409 2476 2561 2735 2959 3872
Size: 989 36 145 67 85 174 224 913
Cuts at: 3872 5282 1239
Size: 1410 2579
Fragments arranged by size:
2579 1410 989 913 224 174 145 85
67 36
Bpu10I CC'TnA_GC
Cuts at: 3813 3813
Size: 6622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2789 3119 4747 5612 5910 2294
Size: 495 330 1628 865 298 3006
Fragments arranged by size:
3006 1628 865 495 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2530 5285 882
Size: 617 1031 2755 2219
Fragments arranged by size:
2755 2219 1031 617
BsaAI yAC'GTr
Cuts at: 493 1204 2098 4413 493
Size: 711 894 2315 2702
Fragments arranged by size:
2702 2315 894 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 3231 4429 4811
Size: 53 83 186 936 765 933 1198 382
Cuts at: 4811 5446 275
Size: 635 1451
Fragments arranged by size:
1451 1198 936 933 765 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3040 5055 5453 5727 5874 6285 3040
Size: 2015 398 274 147 411 3377
Fragments arranged by size:
3377 2015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2382 2412 2915 2945 3389
Size: 30 165 30 979 30 503 30 444
Cuts at: 3389 3419 1178
Size: 30 4381
Fragments arranged by size:
4381 979 503 444 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2264 2518 3869 1186
Size: 1075 3 254 1351 3939
Fragments arranged by size:
3939 1351 1075 254 3
BseYI C'CCAG_C
Cuts at: 2026 2102 2153 3514 5825 6321 2026
Size: 76 51 1361 2311 496 2327
Fragments arranged by size:
2327 2311 1361 496 76 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 2720 3466 1163
Size: 573 768 216 746 4319
Fragments arranged by size:
4319 768 746 573 216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2492 2552 3095 3675 4683 4768 5839 2184
Size: 308 60 543 580 1008 85 1071 2967
Fragments arranged by size:
2967 1071 1008 580 543 308 85 60
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2165 2184 2492 2552 2576 2823
Size: 399 313 62 19 308 60 24 247
Cuts at: 2823 3060 3095 3480 3641 3655 3675 3708 3819
Size: 237 35 385 161 14 20 33 111
Cuts at: 3819 3941 4683 4768 5839 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 313 308 247
237 161 122 111 85 62 60 35
33 24 20 19 14
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 4511 6241 4511
Size: 1730 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2195 2742 2808 3104 3610 3717 3977 844
Size: 1351 547 66 296 506 107 260 3489
Fragments arranged by size:
3489 1351 547 506 296 260 107 66
BsrBI CCG'CTC
Cuts at: 1394 1823 3331 3822 4509 1394
Size: 429 1508 491 687 3507
Fragments arranged by size:
3507 1508 687 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2868 5117 5291 65
Size: 1183 1620 2249 174 1396
Fragments arranged by size:
2249 1620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5266 5453 1700
Size: 110 3456 187 2869
Fragments arranged by size:
3456 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 4682 5694 4682
Size: 1012 5610
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 3896 794
Size: 1280 1822 3520
Fragments arranged by size:
3520 1822 1280
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 2470 1184
Size: 1286 5336
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 2443 2569 2987 3226 3502 3514 2387
Size: 56 126 418 239 276 12 5495
Fragments arranged by size:
5495 418 276 239 126 56 12
BstYI r'GATC_y
Cuts at: 1972 2124 2463 2913 3074 3952 4319 4704 4721
Size: 152 339 450 161 878 367 385 17
Cuts at: 4721 6173 6259 6271 6617 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 878 450 385 367 346 339
161 152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2109 2891 2109
Size: 782 5840
BtgI C'CryG_G
Cuts at: 513 1065 2059 2664 2776 3000 3219 3363 3606
Size: 552 994 605 112 224 219 144 243
Cuts at: 3606 513
Size: 3529
Fragments arranged by size:
3529 994 605 552 243 224 219 144
112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 3890 4138 4932 4952 2222
Size: 1668 248 794 20 3892
Fragments arranged by size:
3892 1668 794 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2782 3147
Size: 54 1425 50 275 18 615 337 365
Cuts at: 3147 3276 3366 3384 3504 3528 4069 4958 6587
Size: 129 90 18 120 24 541 889 1629
Cuts at: 6587 8
Size: 43
Fragments arranged by size:
1629 1425 889 615 541 365 337 275
129 120 90 54 50 43 24 18
18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 4552 1353
Size: 995 2204 3423
Fragments arranged by size:
3423 2204 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5209 5583 5729 387
Size: 1064 138 3620 374 146 1280
Fragments arranged by size:
3620 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2490 2550 2490
Size: 60 6562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2264 2409 2476 2561 2735 2959
Size: 393 596 36 145 67 85 174 224
Cuts at: 2959 3782 3872 4685 5282 6069 6337 1239
Size: 823 90 813 597 787 268 1524
Fragments arranged by size:
1524 823 813 787 597 596 393 268
224 174 145 90 85 67 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2618 3134 2246
Size: 372 516 5734
Fragments arranged by size:
5734 516 372
EcoO109I rG'GnC_Cy
Cuts at: 2031 2113 2133 2162 2203 3321 3651 3652 2031
Size: 82 20 29 41 1118 330 1 5001
Fragments arranged by size:
5001 1118 330 82 41 29 20 1
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 2215 2301 3234 3414 3515 5769 1678
Size: 537 86 933 180 101 2254 2531
Fragments arranged by size:
2531 2254 933 537 180 101 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3389 3421 3682 3714 5263
Size: 32 135 32 1817 32 261 32 1549
Cuts at: 5263 5295 5337 5369 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1817 1549 261 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3966 4212 669
Size: 988 281 2028 246 3079
Fragments arranged by size:
3079 2028 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KasI G'GCGC_C
Cuts at: 2297 3230 2297
Size: 933 5689
KpnI G_GTAC'C
Cuts at: 3472 3947 3472
Size: 475 6147
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 2601 3369 3674 5736 5920 1305
Size: 1155 141 768 305 2062 184 2007
Fragments arranged by size:
2062 2007 1155 768 305 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3149 3386 3506 10
Size: 54 1425 343 1317 237 120 3126
Fragments arranged by size:
3126 1425 1317 343 237 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2567 2606 2951 3023 3224 3500 3605
Size: 648 1401 39 345 72 201 276 105
Cuts at: 3605 3906 4580 4939 5098 518
Size: 301 674 359 159 2042
Fragments arranged by size:
2042 1401 674 648 359 345 301 276
201 159 105 72 39
MspA1I CmG'CkG
Cuts at: 1606 2383 2845 3540 3832 4038 4715 5863 6108
Size: 777 462 695 292 206 677 1148 245
Cuts at: 6108 1606
Size: 2120
Fragments arranged by size:
2120 1148 777 695 677 462 292 245
206
NarI GG'CG_CC
Cuts at: 2298 3231 2298
Size: 933 5689
NcoI C'CATG_G
Cuts at: 513 1065 2776 3000 3219 3606 513
Size: 552 1711 224 219 387 3529
Fragments arranged by size:
3529 1711 552 387 224 219
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 2072 2365 3980 5525 1132
Size: 97 843 293 1615 1545 2229
Fragments arranged by size:
2229 1615 1545 843 293 97
PasI CC'CwG_GG
Cuts at: 1348 3393 3840 1348
Size: 2045 447 4130
Fragments arranged by size:
4130 2045 447
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2060 1108
Size: 952 5670
PfoI T'CCnGG_A
Cuts at: 2205 3849 2205
Size: 1644 4978
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2031 2113 2133 2203 2031
Size: 82 20 70 6450
Fragments arranged by size:
6450 82 70 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PspOMI G'GGCC_C
Cuts at: 2161 3651 2161
Size: 1490 5132
PstI C_TGCA'G
Cuts at: 830 3690 3974 830
Size: 2860 284 3478
Fragments arranged by size:
3478 2860 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2383 2845 2383
Size: 462 6160
SacI G_AGCT'C
Cuts at: 2492 2552 2492
Size: 60 6562
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SanDI GG'GwC_CC
Cuts at: 2031 2031
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 3686 3970 5105 5786 5977 826
Size: 219 2641 284 1135 681 191 1471
Fragments arranged by size:
2641 1471 1135 681 284 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3659 3659
Size: 6622
SfoI GGC'GCC
Cuts at: 2299 3232 2299
Size: 933 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 2130 3703 3949 1872
Size: 258 1573 246 4545
Fragments arranged by size:
4545 1573 258 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 2804 3134 4726 5627 5889
Size: 197 926 366 495 330 1592 901 262
Cuts at: 5889 820
Size: 1553
Fragments arranged by size:
1592 1553 926 901 495 366 330 262
197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 3811 1517
Size: 2294 4328
StyI C'CwwG_G
Cuts at: 513 1065 2776 2986 3000 3150 3219 3507 3606
Size: 552 1711 210 14 150 69 288 99
Cuts at: 3606 4061 513
Size: 455 3074
Fragments arranged by size:
3074 1711 552 455 288 210 150 99
69 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2940 3284 4649 4666 4819 5004 5449
Size: 1255 1360 344 1365 17 153 185 445
Cuts at: 5449 325
Size: 1498
Fragments arranged by size:
1498 1365 1360 1255 445 344 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4868 6580 96
Size: 274 80 33 51 1443 2891 1712 138
Fragments arranged by size:
2891 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2040 2072 2382 2414 2915 2947 3066 3098 6582
Size: 32 310 32 501 32 119 32 3484
Cuts at: 6582 6614 2040
Size: 32 2048
Fragments arranged by size:
3484 2048 501 310 119 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2605 3370 3847 2239
Size: 366 765 477 5014
Fragments arranged by size:
5014 765 477 366
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 2128 3701 3947 1870
Size: 258 1573 246 4545
Fragments arranged by size:
4545 1573 258 246
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SapI SexAI SrfI SwaI