Restriction map for
pFN21AE2413
(Circular) (Six-base) MAPSORT of: pFN21AE2413.seq Check: 4552 from: 1 to: 10636
pFN21AE2413 10636 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 5096 7399 2380
Size: 2716 2303 5617
Fragments arranged by size:
5617 2716 2303
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 8446 278
Size: 53 83 186 936 1819 5091 2468
Fragments arranged by size:
5091 2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5142 5361 7979 1937
Size: 3205 219 2618 4594
Fragments arranged by size:
4594 3205 2618 219
Acc65I G'GTAC_C
Cuts at: 4531 7360 7593 7957 4531
Size: 2829 233 364 7210
Fragments arranged by size:
7210 2829 364 233
AclI AA'CG_TT
Cuts at: 1563 8763 9136 1563
Size: 7200 373 3063
Fragments arranged by size:
7200 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 3312 5841 6050 6629 6839 8699 10083
Size: 405 1275 2529 209 579 210 1860 1384
Cuts at: 10083 10351 1632
Size: 268 1917
Fragments arranged by size:
2529 1917 1860 1384 1275 579 405 268
210 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10439
AflIII A'CryG_T
Cuts at: 1203 3630 4089 4257 5157 5769 9535 10512 1203
Size: 2427 459 168 900 612 3766 977 1327
Fragments arranged by size:
3766 2427 1327 977 900 612 459 168
AgeI A'CCGG_T
Cuts at: 3428 9467 3428
Size: 6039 4597
AhdI GACnn_n'nnGTC
Cuts at: 7870 9365 7870
Size: 1495 9141
AleI CACnn'nnGTG
Cuts at: 1166 3833 1166
Size: 2667 7969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2239 3887 5075 7794 1184
Size: 1055 1648 1188 2719 4026
Fragments arranged by size:
4026 2719 1648 1188 1055
AlwNI CAG_nnn'CTG
Cuts at: 2069 3525 3918 4126 4307 6652 6697 7426 8379
Size: 1456 393 208 181 2345 45 729 953
Cuts at: 8379 9951 2069
Size: 1572 2754
Fragments arranged by size:
2754 2345 1572 1456 953 729 393 208
181 45
ApaI G_GGCC'C
Cuts at: 2100 2555 2922 3758 6371 7089 7497 2100
Size: 455 367 836 2613 718 408 5239
Fragments arranged by size:
5239 2613 836 718 455 408 367
ApaLI G'TGCA_C
Cuts at: 2878 8693 9849 2878
Size: 5815 1156 3665
Fragments arranged by size:
5815 3665 1156
ApoI r'AATT_y
Cuts at: 1493 5078 6386 6749 6788 7204 7486 7945 8171
Size: 3585 1308 363 39 416 282 459 226
Cuts at: 8171 8323 10432 1493
Size: 152 2109 1697
Fragments arranged by size:
3585 2109 1697 1308 459 416 363 282
226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 9190 160
Size: 872 8158 1606
Fragments arranged by size:
8158 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10636
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2211 2665 2771 7740 7961 1870
Size: 73 150 118 454 106 4969 221 4545
Fragments arranged by size:
4969 4545 454 221 150 118 106 73
AvrII C'CTAG_G
Cuts at: 2137 2137
Size: 10636
BamHI G'GATC_C
Cuts at: 2816 5940 7145 7966 8333 2816
Size: 3124 1205 821 367 5119
Fragments arranged by size:
5119 3124 1205 821 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2707 3845 4372 4531 7353
Size: 325 217 627 920 1138 527 159 2822
Cuts at: 7353 7360 7466 7593 7957 9412 618
Size: 7 106 127 364 1455 1842
Fragments arranged by size:
2822 1842 1455 1138 920 627 527 364
325 217 159 127 106 7
BanII G_rGCy'C
Cuts at: 1391 2046 2100 2555 2772 2922 3478 3758 4693
Size: 655 54 455 217 150 556 280 935
Cuts at: 4693 5132 5855 6371 7028 7089 7177 7336 7497
Size: 439 723 516 657 61 88 159 161
Cuts at: 7497 7507 7955 1391
Size: 10 448 4072
Fragments arranged by size:
4072 935 723 657 655 556 516 455
448 439 280 217 161 159 150 88
61 54 10
BbeI G_GCGC'C
Cuts at: 3849 3849
Size: 10636
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 2965 3207 3613 3765 4612 5680
Size: 971 951 115 242 406 152 847 1068
Cuts at: 5680 928
Size: 5884
Fragments arranged by size:
5884 1068 971 951 847 406 242 152
115
BbvCI CC'TCA_GC
Cuts at: 4565 4583 4652 4674 7012 7530 4565
Size: 18 69 22 2338 518 7671
Fragments arranged by size:
7671 2338 518 69 22 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2238 2248 2272 2282 5282 5316 5528
Size: 34 301 10 24 10 3000 34 212
Cuts at: 5528 5562 6498 6532 8827 8861 1903
Size: 34 936 34 2295 34 3678
Fragments arranged by size:
3678 3000 2295 936 301 212 34 34
34 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 6479 8528 9738 6479
Size: 2049 1210 7377
Fragments arranged by size:
7377 2049 1210
BclI T'GATC_A
Cuts at: 1548 6297 6321 1548
Size: 4749 24 5863
Fragments arranged by size:
5863 4749 24
BfrBI ATG'CAT
Cuts at: 5297 5297
Size: 10636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2647 3306
Size: 107 122 71 631 605 388 587 659
Cuts at: 3306 3670 5234 5501 5844 6857 9247 136
Size: 364 1564 267 343 1013 2390 1525
Fragments arranged by size:
2390 1564 1525 1013 659 631 605 587
388 364 343 267 122 107 71
BglII A'GATC_T
Cuts at: 5340 6672 10631 5340
Size: 1332 3959 5345
Fragments arranged by size:
5345 3959 1332
BlpI GC'TnA_GC
Cuts at: 8053 8053
Size: 10636
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2100 2200 2218 2555 2882 2922 3565
Size: 303 7 100 18 337 327 40 643
Cuts at: 3565 3758 5431 6371 6503 7089 7137 7356 7497
Size: 193 1673 940 132 586 48 219 141
Cuts at: 7497 8697 9853 1790
Size: 1200 1156 2573
Fragments arranged by size:
2573 1673 1200 1156 940 643 586 337
327 303 219 193 141 132 100 48
40 18 7
BmgBI CAC'GTC
Cuts at: 1318 2802 5158 1318
Size: 1484 2356 6796
Fragments arranged by size:
6796 2356 1484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2040 3610 3928
Size: 416 53 83 399 24 623 1570 318
Cuts at: 3928 7083 7122 8367 9325 442
Size: 3155 39 1245 958 1753
Fragments arranged by size:
3155 1753 1570 1245 958 623 416 399
318 83 53 39 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 3277 5881 2377
Size: 900 2604 7132
Fragments arranged by size:
7132 2604 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2564 4864 6653 7102 9296 1239
Size: 1166 159 2300 1789 449 2194 2579
Fragments arranged by size:
2579 2300 2194 1789 1166 449 159
Bpu10I CC'TnA_GC
Cuts at: 2148 4565 4583 4652 4674 7012 7506 7530 2148
Size: 2417 18 69 22 2338 494 24 5254
Fragments arranged by size:
5254 2417 2338 494 69 24 22 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3216 4946 5100 5483 8761 9626 9924 2420
Size: 796 1730 154 383 3278 865 298 3132
Fragments arranged by size:
3278 3132 1730 865 796 383 298 154
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3437 3818 7006 7737 9299 882
Size: 617 1938 381 3188 731 1562 2219
Fragments arranged by size:
3188 2219 1938 1562 731 617 381
BsaAI yAC'GTr
Cuts at: 493 1204 2401 7642 8427 493
Size: 711 1197 5241 785 2702
Fragments arranged by size:
5241 2702 1197 785 711
BsaBI GATnn'nnATC
Cuts at: 5393 6662 10630 5393
Size: 1269 3968 5399
Fragments arranged by size:
5399 3968 1269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2571 3111 3352 3846
Size: 53 83 186 936 1038 540 241 494
Cuts at: 3846 6828 8443 8825 9460 275
Size: 2982 1615 382 635 1451
Fragments arranged by size:
2982 1615 1451 1038 936 635 540 494
382 241 186 83 53
BsaWI w'CCGG_w
Cuts at: 3428 4995 6597 9069 9467 9741 9888 10299 3428
Size: 1567 1602 2472 398 274 147 411 3765
Fragments arranged by size:
3765 2472 1602 1567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4787 4817 1178
Size: 30 165 30 3384 30 6997
Fragments arranged by size:
6997 3384 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2849 3479 3482 3881 3980 4097 4412 4445
Size: 1663 630 3 399 99 117 315 33
Cuts at: 4445 4448 4451 4454 4457 6743 6746 6938 6941
Size: 3 3 3 3 2286 3 192 3
Cuts at: 6941 6971 7883 7910 1186
Size: 30 912 27 3912
Fragments arranged by size:
3912 2286 1663 912 630 399 315 192
117 99 33 30 27 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2101 2199 2468 3415 5505 7005 7664 9839 10335
Size: 98 269 947 2090 1500 659 2175 496
Cuts at: 10335 2101
Size: 2402
Fragments arranged by size:
2402 2175 2090 1500 947 659 496 269
98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 2702 4503 4778 4994 5074 5081
Size: 573 921 45 1801 275 216 80 7
Cuts at: 5081 5256 7622 1163
Size: 175 2366 4177
Fragments arranged by size:
4177 2366 1801 921 573 275 216 175
80 45 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8086 8847 9875 1540
Size: 275 186 6085 761 1028 2301
Fragments arranged by size:
6085 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 2463 2685 2739 2882 3012 3654 3876 5721
Size: 27 222 54 143 130 642 222 1845
Cuts at: 5721 5855 7310 7336 8697 8782 9853 2436
Size: 134 1455 26 1361 85 1071 3219
Fragments arranged by size:
3219 1845 1455 1361 1071 642 222 222
143 134 130 85 54 27 26
BsmI GAATG_Cn'
Cuts at: 2796 5126 8147 8240 2796
Size: 2330 3021 93 5192
Fragments arranged by size:
5192 3021 2330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2420 5148 6457 7902 2420
Size: 2728 1309 1445 5154
Fragments arranged by size:
5154 2728 1445 1309
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2093 2100 2200 2218 2436 2463
Size: 399 256 47 7 100 18 218 27
Cuts at: 2463 2555 2685 2739 2772 2882 2922 3012 3478
Size: 92 130 54 33 110 40 90 466
Cuts at: 3478 3565 3654 3758 3876 4693 5132 5431 5721
Size: 87 89 104 118 817 439 299 290
Cuts at: 5721 5855 6371 6503 7028 7089 7137 7177 7310
Size: 134 516 132 525 61 48 40 133
Cuts at: 7310 7336 7356 7497 7507 7955 8697 8782 9853
Size: 26 20 141 10 448 742 85 1071
Cuts at: 9853 1391
Size: 2174
Fragments arranged by size:
2174 1071 817 742 525 516 466 448
439 399 299 290 256 218 141 134
133 132 130 118 110 104 100 92
90 89 87 85 61 54 48 47
40 40 33 27 26 20 18 10
7
BspEI T'CCGG_A
Cuts at: 4995 6597 10299 4995
Size: 1602 3702 5332
Fragments arranged by size:
5332 3702 1602
BspHI T'CATG_A
Cuts at: 2694 3510 5013 5385 8525 10255 2694
Size: 816 1503 372 3140 1730 3075
Fragments arranged by size:
3140 3075 1730 1503 816 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2380 3213 5096 7385 7399 7839 7991 844
Size: 1536 833 1883 2289 14 440 152 3489
Fragments arranged by size:
3489 2289 1883 1536 833 440 152 14
BsrBI CCG'CTC
Cuts at: 1394 1823 5182 8523 1394
Size: 429 3359 3341 3507
Fragments arranged by size:
3507 3359 3341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 2721 4269 4342 5373 5948 6015
Size: 1183 1243 230 1548 73 1031 575 67
Cuts at: 6015 6540 9131 9305 65
Size: 525 2591 174 1396
Fragments arranged by size:
2591 1548 1396 1243 1183 1031 575 525
230 174 73 67
BsrFI r'CCGG_y
Cuts at: 1700 1810 3428 9280 9467 1700
Size: 110 1618 5852 187 2869
Fragments arranged by size:
5852 2869 1618 187 110
BsrGI T'GTAC_A
Cuts at: 96 3488 96
Size: 3392 7244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10636
BssSI C'ACGA_G
Cuts at: 2732 8696 9708 2732
Size: 5964 1012 3660
Fragments arranged by size:
5964 3660 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2680 4307 4972 794
Size: 1886 1627 665 6458
Fragments arranged by size:
6458 1886 1627 665
BstBI TT'CG_AA
Cuts at: 9440 9440
Size: 10636
BstEII G'GTnAC_C
Cuts at: 1184 2205 3907 6736 1184
Size: 1021 1702 2829 5084
Fragments arranged by size:
5084 2829 1702 1021
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 3835 4276 4894 2323
Size: 1512 441 618 8065
Fragments arranged by size:
8065 1512 618 441
BstYI r'GATC_y
Cuts at: 1972 2816 3681 3897 4122 4345 4834 5340 5940
Size: 844 865 216 225 223 489 506 600
Cuts at: 5940 6396 6672 7145 7604 7966 8333 8718 8735
Size: 456 276 473 459 362 367 385 17
Cuts at: 8735 10187 10273 10285 10631 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 865 844 600 506 489 473
459 456 385 367 362 346 276 225
223 216 86 17 12
BstZ17I GTA'TAC
Cuts at: 5143 5143
Size: 10636
Bsu36I CC'TnA_GG
Cuts at: 2352 3893 4542 2352
Size: 1541 649 8446
Fragments arranged by size:
8446 1541 649
BtgI C'CryG_G
Cuts at: 513 1065 2902 2988 3030 3655 4798 5311 5667
Size: 552 1837 86 42 625 1143 513 356
Cuts at: 5667 6741 7669 513
Size: 1074 928 3480
Fragments arranged by size:
3480 1837 1143 1074 928 625 552 513
356 86 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2829 3468 3625 3955 5713 5989
Size: 683 56 1603 639 157 330 1758 276
Cuts at: 5989 6241 487
Size: 252 4882
Fragments arranged by size:
4882 1758 1603 683 639 330 276 252
157 56
BtsI GCAGTG_nn'
Cuts at: 2128 2193 2367 2726 3585 4305 4663 4982 5273
Size: 65 174 359 859 720 358 319 291
Cuts at: 5273 7616 7690 8152 8946 8966 2128
Size: 2343 74 462 794 20 3798
Fragments arranged by size:
3798 2343 859 794 720 462 359 358
319 291 174 74 65 20
ClaI AT'CG_AT
Cuts at: 1554 5292 1554
Size: 3738 6898
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5815 5850 6568 6603 529
Size: 35 5251 35 718 35 4562
Fragments arranged by size:
5251 4562 718 35 35 35
DraI TTT'AAA
Cuts at: 7940 7940
Size: 10636
DraIII CAC_nnn'GTG
Cuts at: 2680 6511 2680
Size: 3831 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9643 809
Size: 507 8327 1802
Fragments arranged by size:
8327 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2235 2561
Size: 54 1425 50 275 18 360 45 326
Cuts at: 2561 2639 2912 3314 3324 3480 4287 4668 4989
Size: 78 273 402 10 156 807 381 321
Cuts at: 4989 5836 7764 8083 8972 10601 8
Size: 847 1928 319 889 1629 43
Fragments arranged by size:
1928 1629 1425 889 847 807 402 381
360 326 321 319 275 273 156 78
54 50 45 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 8083 1537
Size: 275 6271 4090
Fragments arranged by size:
6271 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 2744 3234 3288 3998 4004 4232 4325
Size: 872 519 490 54 710 6 228 93
Cuts at: 4325 4389 4446 4455 4464 4470 5072 5705 5900
Size: 64 57 9 9 6 602 633 195
Cuts at: 5900 6003 6069 6222 6850 6930 7566 7670 8566
Size: 103 66 153 628 80 636 104 896
Cuts at: 8566 1353
Size: 3423
Fragments arranged by size:
3423 896 872 710 636 633 628 602
519 490 228 195 153 104 103 93
80 66 64 57 54 9 9 6
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3253 3527 5504 6811 6838 9223
Size: 1064 138 1664 274 1977 1307 27 2385
Cuts at: 9223 9597 9743 387
Size: 374 146 1280
Fragments arranged by size:
2385 1977 1664 1307 1280 1064 374 274
146 138 27
EcoICRI GAG'CTC
Cuts at: 5853 7334 5853
Size: 1481 9155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2405 2564 3312 4864 5841 6050
Size: 393 405 368 159 748 1552 977 209
Cuts at: 6050 6629 6653 6839 7102 8699 9296 10083 10351
Size: 579 24 186 263 1597 597 787 268
Cuts at: 10351 1239
Size: 1524
Fragments arranged by size:
1597 1552 1524 977 787 748 597 579
405 393 368 268 263 209 186 159
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2387 2387
Size: 10636
EcoO109I rG'GnC_Cy
Cuts at: 2066 2097 2142 2551 2552 2662 2839 2918 3081
Size: 31 45 409 1 110 177 79 163
Cuts at: 3081 3256 3553 3755 4102 4678 4785 6368 6887
Size: 175 297 202 347 576 107 1583 519
Cuts at: 6887 6904 7493 2066
Size: 17 589 5209
Fragments arranged by size:
5209 1583 589 576 519 409 347 297
202 177 175 163 110 107 79 45
31 17 1
EcoRI G'AATT_C
Cuts at: 6386 7945 8323 6386
Size: 1559 378 8699
Fragments arranged by size:
8699 1559 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4069 5149 5767 7426 4069
Size: 1080 618 1659 7279
Fragments arranged by size:
7279 1659 1080 618
FspI TGC'GCA
Cuts at: 7622 9142 7622
Size: 1520 9116
HaeII r_GCGC'y
Cuts at: 1678 2475 2911 3849 4554 4887 7435 9783 1678
Size: 797 436 938 705 333 2548 2348 2531
Fragments arranged by size:
2548 2531 2348 938 797 705 436 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2325 2357 2828 2860 6191
Size: 32 135 32 753 32 471 32 3331
Cuts at: 6191 6223 6289 6321 7061 7093 7856 7888 9277
Size: 32 66 32 740 32 763 32 1389
Cuts at: 9277 9309 9351 9383 1373
Size: 32 42 32 2626
Fragments arranged by size:
3331 2626 1389 763 753 740 471 135
66 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 5194 7980 8226 669
Size: 988 281 1698 1558 2786 246 3079
Fragments arranged by size:
3079 2786 1698 1558 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5918 748
Size: 5170 5466
HpaI GTT'AAC
Cuts at: 8226 8226
Size: 10636
KasI G'GCGC_C
Cuts at: 3845 3845
Size: 10636
KpnI G_GTAC'C
Cuts at: 4535 7364 7597 7961 4535
Size: 2829 233 364 7210
Fragments arranged by size:
7210 2829 364 233
MfeI C'AATT_G
Cuts at: 8235 8235
Size: 10636
MluI A'CGCG_T
Cuts at: 10512 10512
Size: 10636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2388 2844 4812 7173 7379 9750 9934
Size: 684 399 456 1968 2361 206 2371 184
Cuts at: 9934 1305
Size: 2007
Fragments arranged by size:
2371 2361 2007 1968 684 456 399 206
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2237 2914 3482 4289
Size: 54 1425 343 360 45 677 568 807
Cuts at: 4289 4991 5838 7766 10
Size: 702 847 1928 2880
Fragments arranged by size:
2880 1928 1425 847 807 702 677 568
360 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 3833 3869 4274 4614 4892 5057
Size: 648 1350 1317 36 405 340 278 165
Cuts at: 5057 5399 6008 6038 6044 6381 6444 8594 8953
Size: 342 609 30 6 337 63 2150 359
Cuts at: 8953 9112 518
Size: 159 2042
Fragments arranged by size:
2150 2042 1350 1317 648 609 405 359
342 340 337 278 165 159 63 36
30 6
MspA1I CmG'CkG
Cuts at: 1606 2105 2203 2713 2904 3178 3322 3486 4033
Size: 499 98 510 191 274 144 164 547
Cuts at: 4033 4582 4673 4800 7534 7558 7583 8052 8729
Size: 549 91 127 2734 24 25 469 677
Cuts at: 8729 9877 10122 1606
Size: 1148 245 2120
Fragments arranged by size:
2734 2120 1148 677 549 547 510 499
469 274 245 191 164 144 127 98
91 25 24
NarI GG'CG_CC
Cuts at: 3846 3846
Size: 10636
NcoI C'CATG_G
Cuts at: 513 1065 2988 3030 5667 7669 513
Size: 552 1923 42 2637 2002 3480
Fragments arranged by size:
3480 2637 2002 1923 552 42
NdeI CA'TA_TG
Cuts at: 387 4174 5299 387
Size: 3787 1125 5724
Fragments arranged by size:
5724 3787 1125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10636
NotI GC'GGCC_GC
Cuts at: 8083 8083
Size: 10636
NsiI A_TGCA'T
Cuts at: 5299 5299
Size: 10636
NspI r_CATG'y
Cuts at: 1132 1229 4030 4970 6043 6544 7994 9539 1132
Size: 97 2801 940 1073 501 1450 1545 2229
Fragments arranged by size:
2801 2229 1545 1450 1073 940 501 97
PasI CC'CwG_GG
Cuts at: 1348 2329 3048 5431 6416 1348
Size: 981 719 2383 985 5568
Fragments arranged by size:
5568 2383 985 981 719
PciI A'CATG_T
Cuts at: 9535 9535
Size: 10636
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2057 2383 3918 4714 6354 1108
Size: 949 326 1535 796 1640 5390
Fragments arranged by size:
5390 1640 1535 949 796 326
PfoI T'CCnGG_A
Cuts at: 3684 5175 6184 6771 7028 3684
Size: 1491 1009 587 257 7292
Fragments arranged by size:
7292 1491 1009 587 257
PmeI GTTT'AAAC
Cuts at: 7940 7940
Size: 10636
PmlI CAC'GTG
Cuts at: 7642 7642
Size: 10636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7837 7869 8705 8737 10218 10250 7837
Size: 32 836 32 1481 32 8223
Fragments arranged by size:
8223 1481 836 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2142 2662 2839 3081 3256 3553 4678 6887 2142
Size: 520 177 242 175 297 1125 2209 5891
Fragments arranged by size:
5891 2209 1125 520 297 242 177 175
PshAI GACnn'nnGTC
Cuts at: 1613 6885 1613
Size: 5272 5364
PsiI TTA'TAA
Cuts at: 8206 8206
Size: 10636
PspOMI G'GGCC_C
Cuts at: 2096 2551 2918 3754 6367 7085 7493 2096
Size: 455 367 836 2613 718 408 5239
Fragments arranged by size:
5239 2613 836 718 455 408 367
PspXI vC'TCGA_Gb
Cuts at: 2771 2771
Size: 10636
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5576 5608 5576
Size: 32 10604
PstI C_TGCA'G
Cuts at: 830 4506 6879 7396 7431 7988 830
Size: 3676 2373 517 35 557 3478
Fragments arranged by size:
3676 3478 2373 557 517 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10636
PvuII CAG'CTG
Cuts at: 2105 3322 3486 4033 2105
Size: 1217 164 547 8708
Fragments arranged by size:
8708 1217 547 164
SacI G_AGCT'C
Cuts at: 5855 7336 5855
Size: 1481 9155
SacII CC_GC'GG
Cuts at: 2905 4801 2905
Size: 1896 8740
SalI G'TCGA_C
Cuts at: 1936 7978 1936
Size: 6042 4594
SanDI GG'GwC_CC
Cuts at: 2142 2662 2142
Size: 520 10116
SapI GCTCTTCn'nnn_
Cuts at: 2744 3234 4325 5072 6003 6069 2744
Size: 490 1091 747 931 66 7311
Fragments arranged by size:
7311 1091 931 747 490 66
SbfI CC_TGCA'GG
Cuts at: 4506 6879 7396 7988 4506
Size: 2373 517 592 7154
Fragments arranged by size:
7154 2373 592 517
ScaI AGT'ACT
Cuts at: 4914 4923 8884 10596 4914
Size: 9 3961 1712 4954
Fragments arranged by size:
4954 3961 1712 9
SexAI A'CCwGG_T
Cuts at: 7356 7356
Size: 10636
SfcI C'TryA_G
Cuts at: 826 1045 4502 6875 7392 7427 7858 7984 9119
Size: 219 3457 2373 517 35 431 126 1135
Cuts at: 9119 9800 9991 826
Size: 681 191 1471
Fragments arranged by size:
3457 2373 1471 1135 681 517 431 219
191 126 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 3306 3306
Size: 10636
SfoI GGC'GCC
Cuts at: 3847 3847
Size: 10636
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10636
SmaI CCC'GGG
Cuts at: 1872 2095 7963 1872
Size: 223 5868 4545
Fragments arranged by size:
5868 4545 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 2771 3231 4961 5115 5498
Size: 197 926 492 336 460 1730 154 383
Cuts at: 5498 7740 8740 9641 9903 820
Size: 2242 1000 901 262 1553
Fragments arranged by size:
2242 1730 1553 1000 926 901 492 460
383 336 262 197 154
SnaBI TAC'GTA
Cuts at: 493 2401 8427 493
Size: 1908 6026 2702
Fragments arranged by size:
6026 2702 1908
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10636
SphI G_CATG'C
Cuts at: 1132 4030 6043 7994 1132
Size: 2898 2013 1951 3774
Fragments arranged by size:
3774 2898 2013 1951
SspI AAT'ATT
Cuts at: 5 52 8560 5
Size: 47 8508 2081
Fragments arranged by size:
8508 2081 47
StuI AGG'CCT
Cuts at: 1517 2136 4368 5981 1517
Size: 619 2232 1613 6172
Fragments arranged by size:
6172 2232 1613 619
StyI C'CwwG_G
Cuts at: 513 1065 2137 2782 2988 3030 3132 3277 3448
Size: 552 1072 645 206 42 102 145 171
Cuts at: 3448 3831 3858 4311 4518 4953 5667 5842 6423
Size: 383 27 453 207 435 714 175 581
Cuts at: 6423 7215 7669 8075 513
Size: 792 454 406 3074
Fragments arranged by size:
3074 1072 792 714 645 581 552 454
453 435 406 383 207 206 175 171
145 102 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3040 3692 7169 8663 8680 8833
Size: 1255 1437 23 652 3477 1494 17 153
Cuts at: 8833 9018 9463 325
Size: 185 445 1498
Fragments arranged by size:
3477 1498 1494 1437 1255 652 445 185
153 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3054 3488 4912
Size: 274 80 33 51 1443 1077 434 1424
Cuts at: 4912 4921 8882 10594 96
Size: 9 3961 1712 138
Fragments arranged by size:
3961 1712 1443 1424 1077 434 274 138
80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4188 4220 4787 4819 10596 10628 4188
Size: 32 567 32 5777 32 4196
Fragments arranged by size:
5777 4196 567 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5216 1316
Size: 3900 6736
XbaI T'CTAG_A
Cuts at: 7972 7972
Size: 10636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 3856 4525 4633 4750 7831 2380
Size: 1476 669 108 117 3081 5185
Fragments arranged by size:
5185 3081 1476 669 117 108
XhoI C'TCGA_G
Cuts at: 1943 2771 7740 1943
Size: 828 4969 4839
Fragments arranged by size:
4969 4839 828
XmaI C'CCGG_G
Cuts at: 1870 2093 7961 1870
Size: 223 5868 4545
Fragments arranged by size:
5868 4545 223
XmnI GAAnn'nnTTC
Cuts at: 2282 5396 8765 2282
Size: 3114 3369 4153
Fragments arranged by size:
4153 3369 3114
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 8444 276
Size: 53 83 186 936 1819 5091 2468
Fragments arranged by size:
5091 2468 1819 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI RsrII SrfI SwaI